##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934768.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1109976
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13445876307235	33.0	31.0	34.0	30.0	34.0
2	32.305386783137656	34.0	31.0	34.0	30.0	34.0
3	32.305429126395524	34.0	31.0	34.0	30.0	34.0
4	35.88055057046279	37.0	35.0	37.0	35.0	37.0
5	35.7672201921483	37.0	35.0	37.0	35.0	37.0
6	35.7017124694588	37.0	35.0	37.0	33.0	37.0
7	36.03671881193828	37.0	35.0	37.0	35.0	37.0
8	35.87611173574924	37.0	35.0	37.0	35.0	37.0
9	37.52557983235673	39.0	38.0	39.0	35.0	39.0
10	37.28745756665009	39.0	37.0	39.0	34.0	39.0
11	37.1554330904452	39.0	37.0	39.0	34.0	39.0
12	37.02306716541619	39.0	37.0	39.0	33.0	39.0
13	36.941774416744146	39.0	37.0	39.0	33.0	39.0
14	38.04752355005874	40.0	37.0	41.0	33.0	41.0
15	38.133701089032556	40.0	37.0	41.0	33.0	41.0
16	38.222317419475736	40.0	37.0	41.0	34.0	41.0
17	38.19525377125271	40.0	37.0	41.0	34.0	41.0
18	38.171571277216806	40.0	37.0	41.0	34.0	41.0
19	38.14438059922016	40.0	37.0	41.0	34.0	41.0
20	38.07308626492825	40.0	36.0	41.0	34.0	41.0
21	38.025668122554	40.0	36.0	41.0	34.0	41.0
22	37.9692245598103	40.0	36.0	41.0	34.0	41.0
23	37.78881975826504	40.0	36.0	41.0	33.0	41.0
24	37.52031305181373	39.0	35.0	41.0	33.0	41.0
25	37.435405810576086	39.0	35.0	41.0	33.0	41.0
26	37.59397410394459	39.0	35.0	41.0	33.0	41.0
27	37.706410769241856	39.0	36.0	41.0	33.0	41.0
28	37.6665144111224	39.0	36.0	41.0	33.0	41.0
29	37.65203662061162	39.0	36.0	41.0	33.0	41.0
30	37.56789155801567	39.0	36.0	41.0	33.0	41.0
31	37.4767463440651	39.0	35.0	41.0	33.0	41.0
32	37.38391550808306	39.0	35.0	41.0	33.0	41.0
33	37.292603623862135	39.0	35.0	41.0	33.0	41.0
34	37.212033413335064	39.0	35.0	41.0	32.0	41.0
35	37.12080621562989	39.0	35.0	41.0	32.0	41.0
36	37.043978428362415	39.0	35.0	41.0	32.0	41.0
37	37.00954435050848	39.0	35.0	41.0	32.0	41.0
38	36.942260913749486	39.0	35.0	41.0	32.0	41.0
39	36.94749345931804	39.0	35.0	41.0	32.0	41.0
40	36.865482677102925	39.0	35.0	41.0	32.0	41.0
41	36.768904913259384	39.0	35.0	41.0	31.0	41.0
42	36.65721330911659	39.0	35.0	41.0	31.0	41.0
43	36.60310583291891	39.0	35.0	41.0	31.0	41.0
44	36.57098892228301	39.0	35.0	41.0	31.0	41.0
45	36.53248899075295	39.0	35.0	41.0	31.0	41.0
46	36.47853377009953	39.0	35.0	41.0	31.0	41.0
47	36.337950550282166	39.0	35.0	40.0	31.0	41.0
48	36.30575616049356	39.0	35.0	40.0	31.0	41.0
49	36.28869272849143	39.0	35.0	40.0	31.0	41.0
50	36.18971851643639	38.0	35.0	40.0	31.0	41.0
51	35.221184061637366	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	10.0
10	16.0
11	17.0
12	24.0
13	18.0
14	25.0
15	40.0
16	58.0
17	110.0
18	233.0
19	432.0
20	750.0
21	1263.0
22	1847.0
23	2801.0
24	4178.0
25	5913.0
26	8091.0
27	9827.0
28	11089.0
29	12974.0
30	16038.0
31	20166.0
32	26857.0
33	38198.0
34	71350.0
35	96275.0
36	86301.0
37	133045.0
38	237745.0
39	324117.0
40	164.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.449454762985866	22.42625065767188	24.881619062033774	11.242675517308482
2	32.20790359431195	24.519719345283143	25.791098185906726	17.481278874498187
3	30.833459462186568	24.50287213417227	28.11286009787599	16.55080830576517
4	28.102499513502995	25.879928935400404	27.678526382552416	18.339045168544185
5	24.034573720512874	30.415252221669657	26.10849243587249	19.44168162194498
6	26.104258110085265	33.048822677247074	25.58289548602853	15.264023726639135
7	79.5911803498454	5.887154316850094	9.18884732642868	5.332818006875825
8	79.74812068008677	4.704245857568091	9.360833027020405	6.186800435324727
9	73.64618694458258	7.167632453314305	11.743407064657253	7.442773537445854
10	40.43573915111678	25.401269937368014	18.739234001455888	15.423756910059316
11	29.443429407482686	25.335682933685057	24.70873244106179	20.512155217770474
12	27.53230700483614	21.884977693211386	29.648208609915887	20.934506692036585
13	24.079079187297744	25.162886404751095	30.959858591537113	19.798175816414048
14	19.634838951472826	28.709269389608423	29.810824738552906	21.845066920365845
15	18.74265749890088	25.80326061104024	34.994270146381545	20.459811743677342
16	22.16002868530491	25.100002162208913	31.05049118179132	21.689477970694863
17	21.53433948121401	25.624698191672614	27.471224603054477	25.3697377240589
18	22.551478590528085	25.9356058149005	30.121732361780793	21.39118323279062
19	23.275998760333557	27.416899104124777	27.215993859326687	22.09110827621498
20	23.577897179758843	27.929612892531008	27.328518814821223	21.163971112888927
21	22.58138914715273	27.928621880112726	28.814586982060874	20.675401990673674
22	21.37631804651632	25.239014176883103	27.149235659149383	26.23543211745119
23	21.128835218058768	26.38129112701536	28.057633678566024	24.43223997635985
24	22.91004490187175	24.467736239342113	28.679719201135878	23.942499657650256
25	19.841960546894708	27.432304842627225	27.304013780478137	25.421720829999927
26	19.487268193186157	29.34414798157798	28.069255551471382	23.099328273764478
27	22.253994680966073	27.243471930924635	27.79447483549194	22.708058552617356
28	18.423641592250643	26.95472694905115	29.582261238080825	25.039370220617386
29	20.195571796146943	26.459130647869866	29.15720700267393	24.18809055330926
30	22.821304244416098	26.222909324165567	27.335275717673174	23.620510713745162
31	22.384628136103842	27.009953368361117	25.38811649981621	25.217301995718827
32	23.433479642803086	27.27779699741256	26.260657888098482	23.028065471685874
33	23.136806561583313	24.973603032858367	26.514357067179834	25.37523333837849
34	19.592856061752688	25.6409147585173	28.9564819419519	25.80974723777811
35	22.017142712995597	25.711546916329723	27.81033103418452	24.46097933649016
36	22.976352641858924	26.32399259083079	27.415097263364252	23.284557503946033
37	22.0603868912481	24.54890916560358	30.6232747374718	22.76742920567652
38	21.195233050083967	25.583526130294707	27.510594823671862	25.71064599594946
39	21.121537762978658	23.402307797646074	30.715078524220345	24.761075915154922
40	22.66553511066906	23.022569857366285	30.453090877640598	23.858804154324055
41	21.336227089594733	24.721075050271356	28.680349845402063	25.26234801473185
42	22.5895875226131	23.565824846663354	29.220181337254136	24.62440629346941
43	23.013290377449604	22.68967977686004	29.94037708923436	24.356652756455997
44	20.09674083043237	24.08250268474273	28.00096578664764	27.81979069817726
45	21.249288272899594	23.467984893367063	27.966730812197742	27.3159960215356
46	23.701052995740447	23.891958024317642	27.983217655156505	24.423771324785402
47	20.29755598319243	23.787090892055325	31.832670255933458	24.082682868818786
48	21.641368822388955	21.645873424290254	30.83255854180631	25.880199211514483
49	21.789840501055878	20.685942759122717	32.243129581180135	25.28108715864127
50	20.916488284431374	20.861802417349566	31.289144990522317	26.932564307696744
51	20.531254729831996	20.85873928805668	27.995289988251997	30.614715993859328
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	361.0
1	807.5
2	1254.0
3	3812.0
4	6370.0
5	4402.5
6	2435.0
7	2384.5
8	2334.0
9	2417.0
10	2500.0
11	2499.5
12	2499.0
13	2541.0
14	2583.0
15	2558.5
16	2534.0
17	2612.5
18	2691.0
19	2755.0
20	2819.0
21	2775.5
22	2732.0
23	2725.0
24	2718.0
25	3678.0
26	5621.0
27	6604.0
28	8194.5
29	9785.0
30	10756.0
31	11727.0
32	14113.5
33	16500.0
34	18282.5
35	20065.0
36	23184.0
37	26303.0
38	29416.0
39	32529.0
40	38789.5
41	45050.0
42	52575.0
43	60100.0
44	72548.5
45	84997.0
46	99191.0
47	113385.0
48	127479.5
49	141574.0
50	137836.0
51	134098.0
52	112645.0
53	91192.0
54	76795.5
55	62399.0
56	54892.5
57	47386.0
58	44515.5
59	41645.0
60	37343.0
61	33041.0
62	30762.5
63	28484.0
64	25256.5
65	22029.0
66	17999.0
67	13969.0
68	12146.0
69	10323.0
70	8402.0
71	6481.0
72	5774.5
73	5068.0
74	3938.5
75	2365.5
76	1922.0
77	1394.0
78	866.0
79	819.0
80	772.0
81	508.5
82	245.0
83	168.5
84	92.0
85	60.0
86	28.0
87	23.0
88	18.0
89	15.0
90	12.0
91	8.0
92	4.0
93	4.0
94	4.0
95	2.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1109976.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.48293681447524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.49859361087553	20.785055809388112
2	12.542649694897259	7.395882968815062
3	5.175595517590378	4.577752668671952
4	2.6920670329993217	3.174801689370034
5	1.616304192294239	2.3826697187191246
6	1.0347152344397121	1.8303866326776583
7	0.7157315232936751	1.4771307094176875
8	0.5183684881266698	1.2226420305642935
9	0.39095941997753547	1.0373968688599413
>10	3.574163321989395	23.733488933840547
>50	1.0742534565254802	22.210062880670407
>100	0.16073670767082646	7.434484158908315
>500	0.0033936732904808146	0.7230371030208566
>1k	0.0024681260294405925	2.015207827075994
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC	4945	0.44550512803880443	No Hit
CCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC	4222	0.38036858454597217	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3703	0.33361081681045357	No Hit
CTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGCT	2846	0.256401940222131	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC	2109	0.19000410819693397	No Hit
CGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTG	1727	0.15558894967098388	No Hit
ACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC	1573	0.14171477581497258	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.009203802604742E-5	0.0	0.0	0.2694652857359078	0.0
2	9.009203802604742E-5	0.0	0.0	1.4925547939775274	0.0
3	9.009203802604742E-5	0.0	0.0	2.202750329736859	0.0
4	9.009203802604742E-5	0.0	0.0	3.006461400967228	0.0
5	9.009203802604742E-5	0.0	0.0	5.17335509956972	0.0
6	9.009203802604742E-5	0.0	0.0	6.387435404008735	0.0
7	9.009203802604742E-5	0.0	0.0	7.633318197870945	0.0
8	9.009203802604742E-5	0.0	0.0	9.62444233028462	0.0
9	9.009203802604742E-5	0.0	0.0	10.327430503001867	0.0
10	9.009203802604742E-5	0.0	0.0	12.010800233518562	0.0
11	9.009203802604742E-5	0.0	0.0	14.417879305498497	0.0
12	9.009203802604742E-5	0.0	0.0	16.227738257403765	0.0
13	9.009203802604742E-5	0.0	0.0	16.973700332259437	0.0
14	9.009203802604742E-5	0.0	0.0	17.23938175239825	0.0
15	9.009203802604742E-5	0.0	0.0	17.748311675207393	0.0
16	9.009203802604742E-5	0.0	0.0	18.898606816723966	0.0
17	9.009203802604742E-5	0.0	0.0	20.329628748729704	0.0
18	9.009203802604742E-5	0.0	0.0	21.890022847340845	0.0
19	9.009203802604742E-5	0.0	0.0	22.798781234909583	0.0
20	9.009203802604742E-5	0.0	0.0	23.64952034998955	0.0
21	9.009203802604742E-5	0.0	0.0	24.736390696735786	0.0
22	9.009203802604742E-5	0.0	0.0	25.870829639559773	0.0
23	9.009203802604742E-5	0.0	0.0	27.058963437047286	0.0
24	9.009203802604742E-5	0.0	0.0	27.923396541907213	0.0
25	9.009203802604742E-5	0.0	0.0	28.725485956453113	0.0
26	9.009203802604742E-5	0.0	0.0	29.433429191261794	0.0
27	1.8018407605209483E-4	0.0	0.0	30.106596899392418	0.0
28	2.7027611407814224E-4	0.0	0.0	30.833639646262622	0.0
29	2.7027611407814224E-4	0.0	0.0	31.56464644280597	0.0
30	3.6036815210418967E-4	0.0	0.0	32.35142021088744	0.0
31	4.5046019013023704E-4	0.0	0.0	33.10585093731756	0.0
32	4.5046019013023704E-4	0.0	0.0	33.831362119541325	0.0
33	4.5046019013023704E-4	0.0	0.0	34.54056664288237	0.0
34	4.5046019013023704E-4	0.0	0.0	35.17382357816746	0.0
35	4.5046019013023704E-4	0.0	0.0	35.86527997001737	0.0
36	4.5046019013023704E-4	0.0	0.0	36.5841243414272	0.0
37	4.5046019013023704E-4	0.0	0.0	37.286662053954316	0.0
38	4.5046019013023704E-4	0.0	0.0	37.976947249309895	0.0
39	4.5046019013023704E-4	0.0	0.0	38.65092578578275	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	75	0.0	45.000004	25
TTTAGCG	85	0.0	45.000004	1
CGTAGTC	35	1.2118835E-7	45.000004	27
TCTGCGA	20	7.033184E-4	45.0	22
AACCGTG	20	7.033184E-4	45.0	33
ATTAGCG	40	6.8139343E-9	45.0	1
CAACGAT	20	7.033184E-4	45.0	36
CGTGAGA	25	3.8907187E-5	45.0	2
ATTCGCG	40	6.8139343E-9	45.0	1
CGTCTCA	20	7.033184E-4	45.0	13
CGTCGAA	20	7.033184E-4	45.0	41
CCGGTTA	25	3.8907187E-5	45.0	5
ACCGTCC	20	7.033184E-4	45.0	15
TGTACGA	20	7.033184E-4	45.0	34
CGTCCGA	20	7.033184E-4	45.0	2
TGCGTAA	40	6.8139343E-9	45.0	20
CTATCGG	30	2.1654341E-6	44.999996	2
GGTCCGT	30	2.1654341E-6	44.999996	8
ATCGGTA	30	2.1654341E-6	44.999996	14
TACGTTG	100	0.0	42.75	10
>>END_MODULE