##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934767.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	728403
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.927207878056514	33.0	31.0	34.0	30.0	34.0
2	32.12242398781993	33.0	31.0	34.0	30.0	34.0
3	32.133008787717785	34.0	31.0	34.0	30.0	34.0
4	35.74206860762517	37.0	35.0	37.0	33.0	37.0
5	35.60724900913368	37.0	35.0	37.0	33.0	37.0
6	35.5345241576435	37.0	35.0	37.0	33.0	37.0
7	35.94093654199667	37.0	35.0	37.0	35.0	37.0
8	35.795488211882706	37.0	35.0	37.0	35.0	37.0
9	37.56231509205755	39.0	37.0	39.0	35.0	39.0
10	37.184654648594254	39.0	37.0	39.0	34.0	39.0
11	37.06400989562097	39.0	37.0	39.0	33.0	39.0
12	36.96722144197649	39.0	35.0	39.0	33.0	39.0
13	36.79646020128967	39.0	35.0	39.0	33.0	39.0
14	37.80771221425502	40.0	37.0	41.0	33.0	41.0
15	37.89177556929337	40.0	37.0	41.0	33.0	41.0
16	38.022516381728245	40.0	37.0	41.0	33.0	41.0
17	38.034475420886515	40.0	37.0	41.0	34.0	41.0
18	38.02726512658515	39.0	37.0	41.0	34.0	41.0
19	38.003445894648976	40.0	37.0	41.0	34.0	41.0
20	37.91179882565009	39.0	36.0	41.0	34.0	41.0
21	37.83698035290904	39.0	36.0	41.0	34.0	41.0
22	37.77201082367865	39.0	36.0	41.0	33.0	41.0
23	37.570489138567524	39.0	35.0	41.0	33.0	41.0
24	37.30374119821033	39.0	35.0	41.0	32.0	41.0
25	37.21773798295724	39.0	35.0	40.0	32.0	41.0
26	37.381050050590126	39.0	35.0	41.0	33.0	41.0
27	37.51032738744898	39.0	35.0	41.0	33.0	41.0
28	37.519566778280705	39.0	36.0	41.0	33.0	41.0
29	37.526896511958356	39.0	36.0	41.0	33.0	41.0
30	37.37953440609113	39.0	36.0	41.0	33.0	41.0
31	37.28435769759323	39.0	35.0	41.0	33.0	41.0
32	37.135872587015704	39.0	35.0	41.0	32.0	41.0
33	36.96937272361591	39.0	35.0	41.0	32.0	41.0
34	36.9368454001425	39.0	35.0	41.0	32.0	41.0
35	36.85369911985536	39.0	35.0	41.0	31.0	41.0
36	36.736224315385854	39.0	35.0	41.0	31.0	41.0
37	36.69256990978895	39.0	35.0	41.0	31.0	41.0
38	36.62475854712295	39.0	35.0	41.0	31.0	41.0
39	36.654862761410925	39.0	35.0	41.0	31.0	41.0
40	36.561778301297494	39.0	35.0	40.0	31.0	41.0
41	36.51248278768759	39.0	35.0	40.0	31.0	41.0
42	36.40450410006549	39.0	35.0	40.0	30.0	41.0
43	36.35189585984681	39.0	35.0	40.0	30.0	41.0
44	36.30007427207192	39.0	35.0	40.0	30.0	41.0
45	36.29652678530978	39.0	35.0	40.0	30.0	41.0
46	36.24228620694863	39.0	35.0	40.0	30.0	41.0
47	36.09874753398874	39.0	35.0	40.0	30.0	41.0
48	36.09816955723686	39.0	35.0	40.0	30.0	41.0
49	36.09887246483059	39.0	35.0	40.0	30.0	41.0
50	35.9939429134696	39.0	35.0	40.0	29.0	41.0
51	35.0596908579454	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	6.0
10	3.0
11	5.0
12	3.0
13	4.0
14	10.0
15	19.0
16	36.0
17	82.0
18	133.0
19	297.0
20	592.0
21	930.0
22	1481.0
23	2152.0
24	3253.0
25	4852.0
26	6299.0
27	7473.0
28	8088.0
29	9173.0
30	11059.0
31	14370.0
32	18926.0
33	26542.0
34	47275.0
35	65402.0
36	58931.0
37	91146.0
38	165500.0
39	184281.0
40	77.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.79447091788474	24.235759600111475	25.94470368738185	14.025065794621932
2	29.1496602842108	25.137183674421987	26.752223700341705	18.960932341025504
3	29.709652486329684	25.00085804149626	28.839667052442124	16.449822419731934
4	27.428634972673095	25.113707659084326	29.37426122627172	18.08339614197086
5	22.566765924906953	28.471464285567194	29.819344511211515	19.14242527831434
6	23.063606272901126	32.44083289058392	28.320037122307294	16.175523714207657
7	74.33687121003071	5.655660396785845	13.158787099998214	6.848681293185228
8	73.08152217934303	4.025381553892557	13.408099637151413	9.484996629613002
9	68.94658588720804	5.276200125479988	16.606741048567894	9.170472938744075
10	35.695899110794436	22.74482669621075	24.521864956624288	17.037409236370525
11	25.670816841775775	25.960491650913024	27.332259751813215	21.036431755497986
12	24.577740618860712	21.40394808917591	33.876439278805826	20.141872013157553
13	20.144892319224386	26.60656257593667	35.04392486027652	18.20462024456242
14	15.996364649788648	30.285707225258545	31.89347105928998	21.824457065662827
15	13.921002521955566	26.17040292255798	40.9431317553607	18.965462800125753
16	15.167977067639754	25.77034965534189	35.88604110636557	23.175632170652786
17	15.152326390747978	25.87866881382971	30.046553899421063	28.92245089600125
18	16.116353172625594	27.02624783258718	34.95098180540168	21.906417189385547
19	19.187592582677446	27.699501512212333	29.625907636294745	23.486998268815476
20	19.603021953506506	28.222975468250404	31.15280963971867	21.021192938524415
21	18.444048143678703	29.371790066762493	32.5459944563655	19.6381673331933
22	16.767229130028294	26.701015783844934	28.297659400084846	28.234095686041933
23	16.20394204856378	27.70183538508216	30.798884683341505	25.295337883012564
24	20.340113920453376	23.530243560226964	31.35764130570577	24.77200121361389
25	16.154656145018624	28.021164108330144	28.934806693547387	26.889373053103842
26	16.342189694441124	30.57071428865614	29.728598042567096	23.358497974335634
27	22.24016100977069	28.525280648212597	27.760182206827817	21.474376135188898
28	15.274511499815349	26.419029026514167	31.536113936927773	26.770345536742713
29	17.21615644087133	26.06428035030059	31.789270499984212	24.93029270884387
30	22.142550209156195	27.963503719781496	26.968862017317335	22.925084053744975
31	18.773398791603	28.384149982907815	23.411627903784034	29.43082332170516
32	18.748000763313716	29.601333327841868	25.670816841775775	25.979849067068645
33	19.955985903407868	29.806302280468365	24.324858629083078	25.912853187040692
34	15.309519592862742	26.37303800231465	27.650078322027777	30.66736408279483
35	16.719178806237757	26.28380168670365	26.016504599788853	30.98051490726974
36	21.16850150260227	30.090073764111352	24.176863631808214	24.564561101478166
37	17.885840667872046	25.172329054108783	29.914209579037976	27.0276206989812
38	15.772175567645933	28.321135415422503	27.431929852018733	28.47475916491283
39	18.449539609254767	27.96830875216055	29.144855251831746	24.437296386752937
40	20.797415716299906	23.81538791026396	28.940572732402252	26.446623641033877
41	17.309236782385575	26.82471104594572	23.787106862547244	32.07894530912146
42	20.422211330815497	25.715709572860078	26.8855290272006	26.97655006912382
43	23.19842175279344	24.170411159756345	28.19826387315813	24.432903214292086
44	18.717523129366572	24.290125109314488	27.852713401784452	29.139638359534487
45	19.262825661069492	22.613855242221682	27.469134531296547	30.65418456541228
46	22.550154241539367	23.851357009787165	27.804937651272716	25.79355109740075
47	16.40328224897481	23.759786821306335	31.858051106324382	27.978879823394465
48	16.943779748298677	22.603284170987763	29.941392333639484	30.51154374707408
49	19.897364508383408	20.149971924882244	33.837861733134	26.114801833600353
50	19.104396879200113	19.650934990657646	32.001790217777796	29.242877912364445
51	17.298802997791057	19.374165125624142	26.588852599453872	36.738179277130925
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1026.0
1	1021.0
2	1016.0
3	3329.5
4	5643.0
5	3766.5
6	1890.0
7	1983.5
8	2077.0
9	2218.5
10	2360.0
11	2426.5
12	2493.0
13	2595.0
14	2697.0
15	2679.0
16	2661.0
17	2509.5
18	2358.0
19	2330.0
20	2302.0
21	2547.0
22	2792.0
23	2655.0
24	2518.0
25	2540.5
26	3128.5
27	3694.0
28	4527.0
29	5360.0
30	5546.0
31	5732.0
32	7592.0
33	9452.0
34	10383.5
35	11315.0
36	12963.0
37	14611.0
38	16754.0
39	18897.0
40	23622.5
41	28348.0
42	35350.0
43	42352.0
44	51782.5
45	61213.0
46	78305.0
47	95397.0
48	109829.5
49	124262.0
50	117365.5
51	110469.0
52	85953.5
53	61438.0
54	48655.0
55	35872.0
56	28279.0
57	20686.0
58	18395.5
59	16105.0
60	13639.0
61	11173.0
62	9536.5
63	7900.0
64	6029.5
65	4159.0
66	3165.5
67	2172.0
68	1791.5
69	1411.0
70	1106.0
71	801.0
72	725.5
73	650.0
74	472.5
75	229.0
76	163.0
77	102.0
78	41.0
79	35.5
80	30.0
81	16.5
82	3.0
83	2.5
84	2.0
85	3.0
86	4.0
87	2.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	728403.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.82506154075527
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.37491033474318	16.624860210753454
2	12.719016161822422	6.5693875023385
3	6.222289972744863	4.820730644116817
4	3.735104419691363	3.8583720599870586
5	2.4431133914177243	3.154677684220303
6	1.65058123809326	2.5575817231064684
7	1.2360392089869428	2.2344552047211987
8	0.9501738776444211	1.9630639091668196
9	0.6664859341027642	1.5490836237826484
>10	4.95658427129539	26.88141556205381
>50	0.8707019144254254	15.20780874505639
>100	0.15889504726857087	7.626223945975672
>500	0.010199344250347455	1.7755844522014945
>1k	0.0042944607369884025	2.37641073388804
>5k	0.0016104227763706505	2.800343998631339
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC	7629	1.0473597719943493	No Hit
CCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC	7290	1.0008196012372272	No Hit
CTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGCT	5281	0.7250107426795331	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3764	0.5167469107073969	No Hit
TCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC	3547	0.48695570995726273	No Hit
ACTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC	2790	0.38302972393029683	No Hit
CGCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTG	2374	0.32591848193925615	No Hit
GCCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTG	1236	0.16968628630030355	No Hit
ACCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTG	1195	0.1640575340848404	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCC	1121	0.15389832276912643	No Hit
AAAACTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTC	1115	0.15307460293271719	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTC	979	0.13440361997410774	No Hit
GGCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTG	975	0.13385447341650158	No Hit
AGCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTG	941	0.12918672767684922	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	894	0.12273425562497682	No Hit
AGCCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCT	762	0.10461241922397355	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3728663940154007E-4	0.0	0.0	0.7613917021209413	0.0
2	1.3728663940154007E-4	0.0	0.0	3.8443004765219255	0.0
3	1.3728663940154007E-4	0.0	0.0	5.2771611319558	0.0
4	1.3728663940154007E-4	0.0	0.0	6.812712193662025	0.0
5	1.3728663940154007E-4	0.0	0.0	10.819560051235374	0.0
6	1.3728663940154007E-4	0.0	0.0	12.871446163730791	0.0
7	1.3728663940154007E-4	0.0	0.0	15.04290893914495	0.0
8	1.3728663940154007E-4	0.0	0.0	18.47205461811662	0.0
9	1.3728663940154007E-4	0.0	0.0	19.547283577909482	0.0
10	1.3728663940154007E-4	0.0	0.0	22.0540003267422	0.0
11	1.3728663940154007E-4	0.0	0.0	25.721475611714943	0.0
12	1.3728663940154007E-4	0.0	0.0	28.543539771253002	0.0
13	1.3728663940154007E-4	0.0	0.0	29.643754899416944	0.0
14	1.3728663940154007E-4	0.0	0.0	30.029804929414073	0.0
15	1.3728663940154007E-4	0.0	0.0	30.77266293521581	0.0
16	1.3728663940154007E-4	0.0	0.0	32.41927888819788	0.0
17	1.3728663940154007E-4	0.0	0.0	34.39455905590724	0.0
18	1.3728663940154007E-4	0.0	0.0	36.742297876312975	0.0
19	1.3728663940154007E-4	0.0	0.0	37.895093787367706	0.0
20	1.3728663940154007E-4	0.0	0.0	39.00931215275061	0.0
21	1.3728663940154007E-4	0.0	0.0	40.56174947110322	0.0
22	1.3728663940154007E-4	0.0	0.0	42.00133717186777	0.0
23	1.3728663940154007E-4	0.0	0.0	43.52700359553709	0.0
24	1.3728663940154007E-4	0.0	0.0	44.51876227857381	0.0
25	1.3728663940154007E-4	0.0	0.0	45.41908805976911	0.0
26	1.3728663940154007E-4	0.0	0.0	46.20326934403071	0.0
27	1.3728663940154007E-4	0.0	0.0	46.88956525439901	0.0
28	1.3728663940154007E-4	0.0	0.0	47.707930911871586	0.0
29	1.3728663940154007E-4	0.0	0.0	48.49966296130027	0.0
30	1.3728663940154007E-4	0.0	0.0	49.41261911332051	0.0
31	1.3728663940154007E-4	0.0	0.0	50.32530069206195	0.0
32	2.7457327880308014E-4	0.0	0.0	51.0608825059754	0.0
33	2.7457327880308014E-4	0.0	0.0	51.813625149814044	0.0
34	2.7457327880308014E-4	0.0	0.0	52.53506643986914	0.0
35	2.7457327880308014E-4	0.0	0.0	53.32652391601901	0.0
36	2.7457327880308014E-4	0.0	0.0	53.9935996968711	0.0
37	2.7457327880308014E-4	0.0	0.0	54.67509057486035	0.0
38	2.7457327880308014E-4	0.0	0.0	55.34985440751891	0.0
39	2.7457327880308014E-4	0.0	0.0	56.03134528550816	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGTT	35	1.2111013E-7	45.000004	8
ATTACCG	35	1.2111013E-7	45.000004	1
CACGACA	35	1.2111013E-7	45.000004	12
CTGTTAT	35	1.2111013E-7	45.000004	13
CTCGCGC	35	1.2111013E-7	45.000004	33
TGCGTCG	35	1.2111013E-7	45.000004	1
TGATCGG	40	6.8084773E-9	45.0	2
CTTCGTC	20	7.031583E-4	45.0	12
AACGTTT	20	7.031583E-4	45.0	11
CTTAACG	25	3.8893944E-5	45.0	1
TCGTTTG	20	7.031583E-4	45.0	16
TGCCCGA	25	3.8893944E-5	45.0	9
TTAATCG	20	7.031583E-4	45.0	1
GTCGACT	20	7.031583E-4	45.0	9
CGTGCTA	20	7.031583E-4	45.0	16
CGAAAAG	20	7.031583E-4	45.0	2
CGACGGC	20	7.031583E-4	45.0	16
CGCGGAG	25	3.8893944E-5	45.0	30
GGGTCGA	50	2.1827873E-11	45.0	7
TCCGACA	20	7.031583E-4	45.0	18
>>END_MODULE