##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934766.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1065210
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13209132471531	33.0	31.0	34.0	30.0	34.0
2	32.31639676683471	34.0	31.0	34.0	30.0	34.0
3	32.3189643356709	34.0	31.0	34.0	30.0	34.0
4	35.891894556003045	37.0	35.0	37.0	35.0	37.0
5	35.7760357112682	37.0	35.0	37.0	35.0	37.0
6	35.70850630392129	37.0	35.0	37.0	33.0	37.0
7	36.06984819894669	37.0	35.0	37.0	35.0	37.0
8	35.917946695956665	37.0	35.0	37.0	35.0	37.0
9	37.627843336055804	39.0	38.0	39.0	35.0	39.0
10	37.32182480449865	39.0	37.0	39.0	34.0	39.0
11	37.23066531482055	39.0	37.0	39.0	34.0	39.0
12	37.13714009444147	39.0	37.0	39.0	34.0	39.0
13	37.04858948000864	39.0	37.0	39.0	33.0	39.0
14	38.17467917124323	40.0	37.0	41.0	33.0	41.0
15	38.266105275016194	40.0	37.0	41.0	34.0	41.0
16	38.345449254137684	40.0	37.0	41.0	34.0	41.0
17	38.357188723350326	40.0	37.0	41.0	34.0	41.0
18	38.31829780043372	40.0	37.0	41.0	34.0	41.0
19	38.2977656987824	40.0	37.0	41.0	34.0	41.0
20	38.24285164427671	40.0	37.0	41.0	34.0	41.0
21	38.16187887834324	40.0	37.0	41.0	34.0	41.0
22	38.10495770786981	40.0	37.0	41.0	34.0	41.0
23	37.938878718750296	40.0	36.0	41.0	34.0	41.0
24	37.678609851578564	39.0	36.0	41.0	33.0	41.0
25	37.601551806686004	39.0	36.0	41.0	33.0	41.0
26	37.78400784821772	39.0	36.0	41.0	33.0	41.0
27	37.85403253818496	40.0	36.0	41.0	34.0	41.0
28	37.82498098966401	40.0	36.0	41.0	33.0	41.0
29	37.83290806507637	40.0	36.0	41.0	34.0	41.0
30	37.71410895504173	40.0	36.0	41.0	33.0	41.0
31	37.60177054289765	40.0	36.0	41.0	33.0	41.0
32	37.50672355685733	40.0	36.0	41.0	33.0	41.0
33	37.384066052703226	40.0	36.0	41.0	33.0	41.0
34	37.32614789572009	40.0	36.0	41.0	33.0	41.0
35	37.2388402286873	40.0	35.0	41.0	32.0	41.0
36	37.173118915519005	40.0	35.0	41.0	32.0	41.0
37	37.07788698941992	39.0	35.0	41.0	32.0	41.0
38	36.998607786258106	39.0	35.0	41.0	32.0	41.0
39	37.07847372818505	39.0	35.0	41.0	32.0	41.0
40	36.994748453356614	39.0	35.0	41.0	32.0	41.0
41	36.92697684024746	39.0	35.0	41.0	32.0	41.0
42	36.84278311318895	39.0	35.0	41.0	31.0	41.0
43	36.80817773021282	39.0	35.0	41.0	31.0	41.0
44	36.74991973413693	39.0	35.0	41.0	31.0	41.0
45	36.71055660386215	39.0	35.0	41.0	31.0	41.0
46	36.66639911378977	39.0	35.0	41.0	31.0	41.0
47	36.532736267965944	39.0	35.0	41.0	31.0	41.0
48	36.49835994780372	39.0	35.0	41.0	31.0	41.0
49	36.506839965828334	39.0	35.0	41.0	31.0	41.0
50	36.414869368481334	39.0	35.0	40.0	31.0	41.0
51	35.509523004853506	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	7.0
10	15.0
11	11.0
12	13.0
13	13.0
14	20.0
15	15.0
16	53.0
17	73.0
18	201.0
19	352.0
20	589.0
21	1068.0
22	1695.0
23	2506.0
24	3681.0
25	5695.0
26	7624.0
27	9026.0
28	10055.0
29	11741.0
30	14340.0
31	18504.0
32	24713.0
33	34390.0
34	61485.0
35	87128.0
36	81045.0
37	125715.0
38	235741.0
39	327485.0
40	203.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.166455440711225	22.015940518770947	26.706189389885566	12.11141465063227
2	31.975666769932687	24.517512978661486	25.98135578899935	17.52546446240647
3	31.08363609053614	23.270716572319074	28.614263854075723	17.031383483069064
4	27.668534842894825	26.09551168314229	28.291135081345466	17.944818392617417
5	24.48765971029187	29.233578355441647	26.89206823067752	19.386693703588964
6	25.31341237877977	33.079205039381904	25.84814261976512	15.759239962073208
7	80.17414406548944	4.987091747167225	9.772063724523802	5.066700462819538
8	80.9326799410445	3.758507712094329	9.24897438063856	6.059837966222623
9	75.17043587649384	6.281108889326988	11.797673698144028	6.750781536035149
10	37.49166830953521	31.597243735976942	17.504060232254673	13.407027722233176
11	26.129589470620818	25.33322067949043	28.665896865406822	19.871292984481933
12	25.069704565296984	22.255893204156926	32.50739290844059	20.1670093221055
13	22.64436120577163	25.523887308605815	32.73805165178697	19.093699833835583
14	18.95166211357385	29.327174923254567	30.133025412829394	21.588137550342186
15	16.69896076829921	27.399386036556173	35.79538307000497	20.106270125139645
16	19.419175561626346	26.889533519212172	32.19308868673782	21.498202232423655
17	19.731789975685547	25.982388449225972	28.91983740295341	25.36598417213507
18	19.79609654434337	25.5782427878071	32.40365749476629	22.22200317308324
19	21.44412838782963	27.599628242318413	29.46771059227758	21.488532777574378
20	22.885064916776972	27.61765285718309	29.02404220763981	20.473240018400126
21	21.73637123196365	27.914214098628438	30.620722674402227	19.72869199500568
22	19.861060260418135	24.923630082331183	29.354868992968523	25.86044066428216
23	19.873358304935177	26.07166661972757	30.122792688765593	23.93218238657166
24	21.57856197369533	24.376883431436056	30.899165422780484	23.145389172088134
25	19.157161498671623	27.57193417260446	29.17715755578712	24.093746772936793
26	19.22334563137785	27.76870288487716	30.35335755390956	22.654593929835432
27	21.53256165450944	27.327756968109572	29.75863914157772	21.381042235803267
28	18.199322199378525	27.137653608208712	31.397470921226798	23.26555327118596
29	20.663155621896152	24.511035382694494	31.634607260540175	23.19120173486918
30	24.448512499882654	25.575895832746593	28.974380638559534	21.00121102881122
31	22.76846818937111	28.260155274546804	25.87602444588391	23.095352090198176
32	24.179363693543994	27.615212023920165	26.22872485237653	21.97669943015931
33	24.5664235221224	26.18516536645356	27.018991560349605	22.229419551074436
34	20.9806517024812	25.669867913369192	28.73752593385342	24.611954450296185
35	22.131504585950186	25.142460172172626	28.446127993541182	24.27990724833601
36	23.815773415570636	25.67240262483454	29.670675265910006	20.841148693684815
37	21.79363693543996	27.74410679584307	29.046760732625494	21.415495536091473
38	21.552463833422518	28.921433332394553	26.842969930811766	22.683132903371167
39	23.72320950798434	24.037513729687106	29.284929732165487	22.954347030163067
40	23.17552407506501	23.959688699880775	29.197623003914718	23.667164221139494
41	19.37871405638325	25.718778456830112	28.599994367307858	26.30251311947879
42	21.452765182452286	25.03619004703298	30.593967386712478	22.917077383802255
43	23.74874437904263	24.528872241154325	29.320321814477897	22.402061565325145
44	22.328930445639827	24.521268106758292	28.127317618122248	25.022483829479636
45	22.948338825208175	23.7323156936191	27.187502933693825	26.1318425474789
46	24.398663174397537	24.11871837478056	28.26813492175252	23.214483529069383
47	20.310079702593857	24.607448296580017	31.13780381333258	23.944668187493544
48	20.841618084696915	23.75756893007013	30.558669182602493	24.842143802630467
49	22.155725162174594	21.771012288656696	32.40731874466068	23.66594380450803
50	21.71515475821669	21.764722449094545	30.826128181297584	25.69399461139118
51	20.648604500521024	21.515475821668968	28.531275523136284	29.304644154673724
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	511.0
1	744.0
2	977.0
3	4059.0
4	7141.0
5	4806.5
6	2472.0
7	2530.0
8	2588.0
9	2653.0
10	2718.0
11	2757.5
12	2797.0
13	2766.5
14	2736.0
15	2732.5
16	2729.0
17	2596.0
18	2463.0
19	2505.0
20	2547.0
21	2787.5
22	3028.0
23	3764.5
24	4501.0
25	4767.5
26	6277.0
27	7520.0
28	8957.5
29	10395.0
30	12985.5
31	15576.0
32	17247.5
33	18919.0
34	21208.5
35	23498.0
36	24801.5
37	26105.0
38	30244.5
39	34384.0
40	40276.5
41	46169.0
42	55015.5
43	63862.0
44	74285.0
45	84708.0
46	98064.0
47	111420.0
48	129140.5
49	146861.0
50	139710.0
51	132559.0
52	108638.0
53	84717.0
54	71831.0
55	58945.0
56	50179.5
57	41414.0
58	36817.5
59	32221.0
60	29958.5
61	27696.0
62	23753.5
63	19811.0
64	16513.0
65	13215.0
66	10772.0
67	8329.0
68	6980.0
69	5631.0
70	4592.5
71	3554.0
72	2997.5
73	2441.0
74	2007.0
75	1168.0
76	763.0
77	542.0
78	321.0
79	245.0
80	169.0
81	101.0
82	33.0
83	44.0
84	55.0
85	32.0
86	9.0
87	47.5
88	86.0
89	45.5
90	5.0
91	4.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1065210.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.3372772127329
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.35774361535886	17.74019566021896
2	12.735835642391443	6.708544676989353
3	5.791584163399912	4.576036728370116
4	3.165110313936371	3.33441550988089
5	1.9771489636280184	2.6036360172969344
6	1.2898930851881847	2.038336305563311
7	0.9332882224562663	1.7206189443946591
8	0.687731176592661	1.4490373316607903
9	0.5135171336736858	1.217217879274669
>10	4.0207079334265785	23.815882651414864
>50	1.3492685280619279	24.751657520275696
>100	0.17095781209319458	6.824708060035968
>500	0.0043280458757754756	0.7777845613612504
>1k	0.0025246934275356945	1.9144443148112655
>5k	3.606704896479563E-4	0.527483838451273
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC	5553	0.5213056580392599	No Hit
CCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC	4761	0.4469541217224772	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4482	0.420762103247247	No Hit
CTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGCT	3923	0.36828418809436636	No Hit
TCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC	2437	0.2287811792979788	No Hit
ACTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC	1839	0.17264201425071113	No Hit
CGCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTG	1629	0.1529275917424733	No Hit
ACCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTG	1083	0.101670093221055	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3876230977929235	0.0
2	0.0	0.0	0.0	1.8408576712573108	0.0
3	0.0	0.0	0.0	2.602773162099492	0.0
4	0.0	0.0	0.0	3.494803841496043	0.0
5	0.0	0.0	0.0	5.712113104458276	0.0
6	0.0	0.0	0.0	6.904741788004244	0.0
7	0.0	0.0	0.0	8.166464828531463	0.0
8	0.0	0.0	0.0	10.215825987363994	0.0
9	0.0	0.0	0.0	10.953802536589029	0.0
10	0.0	0.0	0.0	12.541189061311854	0.0
11	0.0	0.0	0.0	14.858103097041898	0.0
12	0.0	0.0	0.0	16.68412801231682	0.0
13	0.0	0.0	0.0	17.379108344833412	0.0
14	0.0	0.0	0.0	17.631640709343696	0.0
15	0.0	0.0	0.0	18.115582842819727	0.0
16	0.0	0.0	0.0	19.179880023657308	0.0
17	0.0	0.0	0.0	20.58251424601722	0.0
18	0.0	0.0	0.0	22.16426807859483	0.0
19	0.0	0.0	0.0	23.03376798941054	0.0
20	0.0	0.0	0.0	23.907210784727894	0.0
21	0.0	0.0	0.0	24.940903671576496	0.0
22	0.0	0.0	0.0	26.066127805784774	0.0
23	0.0	0.0	0.0	27.21538475981262	0.0
24	0.0	0.0	0.0	28.078031561851652	0.0
25	0.0	0.0	0.0	28.813661156016185	0.0
26	0.0	0.0	0.0	29.503384309197248	0.0
27	0.0	0.0	0.0	30.15705823264896	0.0
28	0.0	0.0	0.0	30.884426545000515	0.0
29	0.0	0.0	0.0	31.64671754865238	0.0
30	0.0	0.0	0.0	32.45491499328771	0.0
31	0.0	0.0	0.0	33.2024671191596	0.0
32	1.8775640484036012E-4	0.0	0.0	33.90664751551337	0.0
33	1.8775640484036012E-4	0.0	0.0	34.60435031590015	0.0
34	1.8775640484036012E-4	0.0	0.0	35.28975507176989	0.0
35	1.8775640484036012E-4	0.0	0.0	36.03862149247566	0.0
36	1.8775640484036012E-4	0.0	0.0	36.71266698585256	0.0
37	1.8775640484036012E-4	0.0	0.0	37.41140244646596	0.0
38	1.8775640484036012E-4	0.0	0.0	38.09896640099135	0.0
39	1.8775640484036012E-4	0.0	0.0	38.77554660583359	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAACGG	70	0.0	45.000004	2
AACGCGA	30	2.1653523E-6	45.000004	24
ACTCGAC	30	2.1653523E-6	45.000004	1
CGCGTTG	30	2.1653523E-6	45.000004	25
ACGCGAA	25	3.8906124E-5	45.0	35
TCGCCTA	20	7.033055E-4	45.0	41
TAATACG	25	3.8906124E-5	45.0	1
TCGCAAA	20	7.033055E-4	45.0	30
GCGGTTA	20	7.033055E-4	45.0	20
GATTTCG	20	7.033055E-4	45.0	9
TGAAACG	20	7.033055E-4	45.0	1
TCTAGCG	40	6.8139343E-9	45.0	1
CCCGTTG	20	7.033055E-4	45.0	1
CCGTACG	25	3.8906124E-5	45.0	23
CCGTACA	25	3.8906124E-5	45.0	31
TCTACGA	20	7.033055E-4	45.0	11
GTTCGGT	20	7.033055E-4	45.0	11
TTAAGCG	40	6.8139343E-9	45.0	1
GAACGTA	25	3.8906124E-5	45.0	9
CTACCGT	20	7.033055E-4	45.0	21
>>END_MODULE