SRR2934763.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934763.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	598447
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	3495	0.5840116167346482	Illumina PCR Primer Index 7 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	3183	0.5318766741248598	Illumina PCR Primer Index 7 (96% over 27bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2778	0.46420150823715384	No Hit
CTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGCT	2507	0.41891763180365177	Illumina PCR Primer Index 7 (96% over 28bp)
TCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	1425	0.23811632441970634	Illumina PCR Primer Index 7 (96% over 27bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC	1186	0.19817962158720825	No Hit
ACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	1146	0.1914956545859533	Illumina PCR Primer Index 7 (96% over 27bp)
CGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTG	1144	0.19116145623589056	Illumina PCR Primer Index 7 (96% over 26bp)
GAATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC	1133	0.18932336531054547	Illumina PCR Primer Index 7 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG	1126	0.18815367108532585	No Hit
AGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTG	600	0.10025950501882372	Illumina PCR Primer Index 7 (96% over 26bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTA	30	2.1637516E-6	45.000004	38
GCGATCA	30	2.1637516E-6	45.000004	9
GTATCGG	60	0.0	45.000004	2
TCACGCG	20	7.0305733E-4	45.0	1
TCACGAC	55	1.8189894E-12	45.0	25
TCGCCAG	35	1.2106284E-7	45.0	25
TTGGCGT	25	3.888556E-5	45.0	19
GGTACGT	20	7.0305733E-4	45.0	9
CCCCTCG	20	7.0305733E-4	45.0	43
CCCTCGT	20	7.0305733E-4	45.0	14
CTCGTAG	25	3.888556E-5	45.0	1
CGACGGT	55	1.8189894E-12	45.0	28
GCGAAGA	20	7.0305733E-4	45.0	25
GCGAACC	20	7.0305733E-4	45.0	13
CGGTTTG	20	7.0305733E-4	45.0	44
TAGGTCG	20	7.0305733E-4	45.0	2
CGGTTAT	20	7.0305733E-4	45.0	18
GGCGTAG	35	1.2106284E-7	45.0	1
TAACGTG	20	7.0305733E-4	45.0	22
TCGATTT	25	3.888556E-5	45.0	12