##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934763.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	598447
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16528614898228	33.0	31.0	34.0	30.0	34.0
2	32.34704827662266	34.0	31.0	34.0	30.0	34.0
3	32.35173707947404	34.0	31.0	34.0	30.0	34.0
4	35.90721317008858	37.0	35.0	37.0	35.0	37.0
5	35.788901941191114	37.0	35.0	37.0	35.0	37.0
6	35.732223572012224	37.0	35.0	37.0	35.0	37.0
7	36.03566731891045	37.0	35.0	37.0	35.0	37.0
8	35.85107453124504	37.0	35.0	37.0	35.0	37.0
9	37.56952746024293	39.0	38.0	39.0	35.0	39.0
10	37.32626281024051	39.0	37.0	39.0	34.0	39.0
11	37.18465127237667	39.0	37.0	39.0	34.0	39.0
12	37.03208137061427	39.0	37.0	39.0	33.0	39.0
13	36.89915564786857	39.0	35.0	39.0	33.0	39.0
14	37.97911427411283	40.0	37.0	41.0	33.0	41.0
15	38.094130307278675	40.0	37.0	41.0	33.0	41.0
16	38.2125033628709	40.0	37.0	41.0	34.0	41.0
17	38.215517831988464	40.0	37.0	41.0	34.0	41.0
18	38.19897167167686	40.0	37.0	41.0	34.0	41.0
19	38.15812929131569	40.0	37.0	41.0	34.0	41.0
20	38.093563841075316	40.0	36.0	41.0	34.0	41.0
21	38.03624214007255	40.0	36.0	41.0	34.0	41.0
22	37.9595218958404	40.0	36.0	41.0	34.0	41.0
23	37.771486865169344	40.0	35.0	41.0	33.0	41.0
24	37.52884382409804	39.0	35.0	41.0	33.0	41.0
25	37.45323813136334	39.0	35.0	41.0	33.0	41.0
26	37.566800401706416	39.0	35.0	41.0	33.0	41.0
27	37.65588097191564	39.0	35.0	41.0	33.0	41.0
28	37.60076999299854	39.0	35.0	41.0	33.0	41.0
29	37.60523321196363	39.0	36.0	41.0	33.0	41.0
30	37.51340887330039	39.0	36.0	41.0	33.0	41.0
31	37.34694467513414	39.0	35.0	41.0	33.0	41.0
32	37.26999550503219	39.0	35.0	41.0	33.0	41.0
33	37.17431284641748	39.0	35.0	41.0	33.0	41.0
34	37.065870494797366	39.0	35.0	41.0	32.0	41.0
35	37.045928879249125	39.0	35.0	41.0	32.0	41.0
36	36.939632081036414	39.0	35.0	41.0	32.0	41.0
37	36.82933994154871	39.0	35.0	41.0	31.0	41.0
38	36.76898204853563	39.0	35.0	41.0	31.0	41.0
39	36.82939842625997	39.0	35.0	41.0	31.0	41.0
40	36.6985564302269	39.0	35.0	41.0	31.0	41.0
41	36.64015693954519	39.0	35.0	41.0	31.0	41.0
42	36.56211995381379	39.0	35.0	41.0	31.0	41.0
43	36.50760886093505	39.0	35.0	41.0	31.0	41.0
44	36.41746052699738	39.0	35.0	41.0	31.0	41.0
45	36.377309937220836	39.0	35.0	41.0	31.0	41.0
46	36.295680319226264	39.0	35.0	40.0	30.0	41.0
47	36.13241941224536	38.0	35.0	40.0	30.0	41.0
48	36.12475791507018	38.0	35.0	40.0	30.0	41.0
49	36.13026049090396	38.0	35.0	40.0	30.0	41.0
50	36.022834102268035	38.0	35.0	40.0	30.0	41.0
51	35.00617932749266	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	11.0
10	9.0
11	9.0
12	11.0
13	11.0
14	15.0
15	15.0
16	32.0
17	77.0
18	166.0
19	266.0
20	460.0
21	768.0
22	1169.0
23	1705.0
24	2405.0
25	3273.0
26	4456.0
27	5440.0
28	6121.0
29	7182.0
30	8704.0
31	11059.0
32	14597.0
33	20385.0
34	38756.0
35	57429.0
36	45793.0
37	69935.0
38	125352.0
39	172716.0
40	119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.27432170267375	21.63115530698625	25.452379241603683	13.642143748736313
2	31.253394201992823	26.02285582516079	26.071982982620014	16.65176699022637
3	31.392253616443895	24.782979946428004	28.047596529015934	15.777169908112162
4	27.560001136274394	25.791256368567307	27.916089478266247	18.732653016892055
5	25.103977461663273	29.757522387111973	25.262554578768043	19.875945572456708
6	24.817068178134406	34.214725781898814	24.964616749687107	16.003589290279674
7	78.2998327337258	5.682374546116866	10.575372589385527	5.442420130771814
8	78.11351715356581	5.160189624143825	9.930369773764427	6.795923448525934
9	73.1446560848329	6.724739199962569	12.375866200348568	7.754738514855952
10	39.38661234829483	26.983843180766215	19.595386057579034	14.034158413359913
11	28.848669974116337	24.399153141380943	27.366500291588057	19.38567659291466
12	27.24618888556547	21.61912416638399	31.702222586127093	19.432464361923447
13	22.543182604307482	25.95651745267334	32.57180669299036	18.928493250028826
14	17.43947250132426	32.09356885405057	29.387731912767546	21.079226731857624
15	16.294676053184325	25.872466567632557	38.4293011745401	19.403556204643017
16	17.805252595467934	25.57586553195187	35.02415418575078	21.59472768682941
17	17.625286783959147	24.85382999664131	29.74615964320984	27.774723576189704
18	19.13619752459282	26.221035446747997	32.62845331332599	22.014313715333188
19	21.1211686247905	28.061131562193474	29.185040613454493	21.632659199561534
20	23.704856069125587	27.391899366192828	28.326150853793237	20.57709371088835
21	21.533402289592896	27.7778984605153	30.06348097659442	20.625218273297385
22	19.12550317739081	25.829522079649493	28.40936624295886	26.635608500000835
23	18.258926855678116	27.855432477729856	28.933723454207303	24.95191721238472
24	21.223767518259763	25.86561550145627	29.91376011576631	22.99685686451766
25	18.8314086293356	27.646892707290704	28.70646857616464	24.81523008720906
26	17.810933967419	30.591681468868586	29.016270446672802	22.581114117039604
27	21.734923894680733	29.22597991133718	27.905562230239266	21.13353396374282
28	17.990398481402696	28.430086540662746	30.982359340091936	22.59715563784262
29	20.565396768636155	27.346448390584293	29.084112711735543	23.00404212904401
30	22.883062326321298	27.370677770963848	27.213604546434354	22.532655356280507
31	23.193532593529586	31.568710345277022	22.966277715486918	22.27147934570647
32	24.224367404298125	29.743486056409342	24.095701039523966	21.936445499768567
33	22.625228299247887	27.12153290099207	24.34818789299637	25.90505090676367
34	19.779529348463605	29.15696795204922	27.63603126091366	23.42747143857351
35	21.07237566568134	25.914909758090527	26.68105947560937	26.331655100618768
36	22.23354783297435	27.981091057353453	25.48462938238474	24.300731727287463
37	21.86225346605464	29.162482224825254	28.537865508557985	20.43739880056212
38	19.793565679166242	28.72601917964331	26.13364257820659	25.346772562983855
39	21.843872556801188	25.213761619658886	26.999550503219165	25.94281532032076
40	21.980225483626786	25.010234824470672	29.368682606813973	23.64085708508857
41	18.1773824582628	26.734865409969473	28.30258987011381	26.785162261653916
42	21.29729115527357	25.665430689768687	27.891860097886696	25.14541805707105
43	23.713043928702124	24.580789944640046	27.52791809466837	24.178248031989465
44	21.38167623866441	26.061789932943103	26.593833706242993	25.962700122149496
45	21.434312478799292	25.282439380596777	26.111752586277483	27.171495554326448
46	23.486457447359584	25.880320228859034	27.324892597005245	23.30832972677614
47	19.219245814583413	26.054938866766815	30.793704371481518	23.932110947168255
48	20.240890170725226	23.497485992911653	29.463260739881726	26.798363096481392
49	20.76959196052449	21.54125595081937	31.75001295018606	25.939139138470075
50	20.22651964167253	22.780797631202095	29.97140933115213	27.021273395973246
51	19.139038210568355	23.282262255471245	26.859855592892938	30.718843941067465
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	447.0
1	620.5
2	794.0
3	2596.5
4	4399.0
5	2979.5
6	1560.0
7	1559.0
8	1558.0
9	1532.5
10	1507.0
11	1534.5
12	1562.0
13	1516.5
14	1471.0
15	1452.5
16	1434.0
17	1423.0
18	1412.0
19	1424.0
20	1436.0
21	1414.0
22	1392.0
23	1694.5
24	1997.0
25	2793.0
26	3732.5
27	3876.0
28	5040.5
29	6205.0
30	7630.5
31	9056.0
32	9876.0
33	10696.0
34	12233.5
35	13771.0
36	15556.0
37	17341.0
38	18144.0
39	18947.0
40	22375.0
41	25803.0
42	31049.0
43	36295.0
44	42064.5
45	47834.0
46	57177.0
47	66520.0
48	72582.0
49	78644.0
50	74969.0
51	71294.0
52	57623.0
53	43952.0
54	36677.0
55	29402.0
56	25222.0
57	21042.0
58	20131.0
59	19220.0
60	18098.5
61	16977.0
62	14449.5
63	11922.0
64	10670.5
65	9419.0
66	7065.5
67	4712.0
68	3733.5
69	2755.0
70	2822.0
71	2889.0
72	2523.0
73	2157.0
74	1837.5
75	1148.0
76	778.0
77	649.0
78	520.0
79	315.0
80	110.0
81	93.0
82	76.0
83	76.0
84	76.0
85	77.0
86	78.0
87	40.0
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	598447.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.26549744967936
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.60297003000298	18.704941043934756
2	12.751223949424284	6.953369281466365
3	5.436514858219978	4.446878460058222
4	2.931492953289729	3.1971445456669647
5	1.7713850998303682	2.414884796091246
6	1.1552486463341232	1.8899057412221136
7	0.8071783872856854	1.540568418198191
8	0.6090269276856747	1.3284337714879855
9	0.45530547776454816	1.11727173085329
>10	4.100118656101379	26.637537362960373
>50	1.2600331030586358	22.893546481299502
>100	0.11145255948430195	5.348979969422621
>500	0.0018575426580716994	0.29814097418256374
>1k	0.006191808860238998	3.2283974231558132
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	3495	0.5840116167346482	Illumina PCR Primer Index 7 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	3183	0.5318766741248598	Illumina PCR Primer Index 7 (96% over 27bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2778	0.46420150823715384	No Hit
CTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGCT	2507	0.41891763180365177	Illumina PCR Primer Index 7 (96% over 28bp)
TCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	1425	0.23811632441970634	Illumina PCR Primer Index 7 (96% over 27bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC	1186	0.19817962158720825	No Hit
ACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	1146	0.1914956545859533	Illumina PCR Primer Index 7 (96% over 27bp)
CGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTG	1144	0.19116145623589056	Illumina PCR Primer Index 7 (96% over 26bp)
GAATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC	1133	0.18932336531054547	Illumina PCR Primer Index 7 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG	1126	0.18815367108532585	No Hit
AGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTG	600	0.10025950501882372	Illumina PCR Primer Index 7 (96% over 26bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.3419835006274573E-4	0.0	0.0	0.44983097918445575	0.0
2	3.3419835006274573E-4	0.0	0.0	2.099099836744106	0.0
3	3.3419835006274573E-4	0.0	0.0	2.924235563049025	0.0
4	3.3419835006274573E-4	0.0	0.0	3.850132091897862	0.0
5	3.3419835006274573E-4	0.0	0.0	6.4014022962768635	0.0
6	3.3419835006274573E-4	0.0	0.0	7.7224883740748975	0.0
7	3.3419835006274573E-4	0.0	0.0	9.193629511051103	0.0
8	3.3419835006274573E-4	0.0	0.0	11.397166332189817	0.0
9	3.3419835006274573E-4	0.0	0.0	12.162313454658474	0.0
10	3.3419835006274573E-4	0.0	0.0	13.959799280470952	0.0
11	3.3419835006274573E-4	0.0	0.0	16.34630969826902	0.0
12	3.3419835006274573E-4	0.0	0.0	18.40948321238138	0.0
13	3.3419835006274573E-4	0.0	0.0	19.1238321856405	0.0
14	3.3419835006274573E-4	0.0	0.0	19.401718113717674	0.0
15	3.3419835006274573E-4	0.0	0.0	19.91755326703952	0.0
16	3.3419835006274573E-4	0.0	0.0	21.015060648645576	0.0
17	3.3419835006274573E-4	0.0	0.0	22.402652198106097	0.0
18	3.3419835006274573E-4	0.0	0.0	24.023514195910415	0.0
19	5.012975250941186E-4	0.0	0.0	24.870874112494505	0.0
20	5.012975250941186E-4	0.0	0.0	25.60794857355789	0.0
21	5.012975250941186E-4	0.0	0.0	26.56292035886219	0.0
22	5.012975250941186E-4	0.0	0.0	27.525077408692834	0.0
23	5.012975250941186E-4	0.0	0.0	28.50444567355171	0.0
24	5.012975250941186E-4	0.0	0.0	29.292485382999665	0.0
25	5.012975250941186E-4	0.0	0.0	29.902230272689145	0.0
26	5.012975250941186E-4	0.0	0.0	30.491589063024797	0.0
27	5.012975250941186E-4	0.0	0.0	31.04117824970298	0.0
28	5.012975250941186E-4	0.0	0.0	31.65810840391881	0.0
29	5.012975250941186E-4	0.0	0.0	32.25231307033037	0.0
30	5.012975250941186E-4	0.0	0.0	32.94560754753554	0.0
31	5.012975250941186E-4	0.0	0.0	33.58927356975639	0.0
32	5.012975250941186E-4	0.0	0.0	34.17061159969053	0.0
33	5.012975250941186E-4	0.0	0.0	34.757463902400715	0.0
34	5.012975250941186E-4	0.0	0.0	35.32025392390638	0.0
35	5.012975250941186E-4	0.0	0.0	35.940860259972894	0.0
36	5.012975250941186E-4	0.0	0.0	36.530051951133515	0.0
37	5.012975250941186E-4	0.0	0.0	37.07763594771133	0.0
38	5.012975250941186E-4	0.0	0.0	37.61653078718751	0.0
39	5.012975250941186E-4	0.0	0.0	38.14924295718752	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTA	30	2.1637516E-6	45.000004	38
GCGATCA	30	2.1637516E-6	45.000004	9
GTATCGG	60	0.0	45.000004	2
TCACGCG	20	7.0305733E-4	45.0	1
TCACGAC	55	1.8189894E-12	45.0	25
TCGCCAG	35	1.2106284E-7	45.0	25
TTGGCGT	25	3.888556E-5	45.0	19
GGTACGT	20	7.0305733E-4	45.0	9
CCCCTCG	20	7.0305733E-4	45.0	43
CCCTCGT	20	7.0305733E-4	45.0	14
CTCGTAG	25	3.888556E-5	45.0	1
CGACGGT	55	1.8189894E-12	45.0	28
GCGAAGA	20	7.0305733E-4	45.0	25
GCGAACC	20	7.0305733E-4	45.0	13
CGGTTTG	20	7.0305733E-4	45.0	44
TAGGTCG	20	7.0305733E-4	45.0	2
CGGTTAT	20	7.0305733E-4	45.0	18
GGCGTAG	35	1.2106284E-7	45.0	1
TAACGTG	20	7.0305733E-4	45.0	22
TCGATTT	25	3.888556E-5	45.0	12
>>END_MODULE