##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934761.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	925536
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11354609653217	33.0	31.0	34.0	30.0	34.0
2	32.285655015039936	34.0	31.0	34.0	30.0	34.0
3	32.290093524184904	34.0	31.0	34.0	30.0	34.0
4	35.87247173529717	37.0	35.0	37.0	35.0	37.0
5	35.75921628116032	37.0	35.0	37.0	35.0	37.0
6	35.687556183660064	37.0	35.0	37.0	33.0	37.0
7	36.05021954845625	37.0	35.0	37.0	35.0	37.0
8	35.88750950800401	37.0	35.0	37.0	35.0	37.0
9	37.59164095183764	39.0	38.0	39.0	35.0	39.0
10	37.316623016284616	39.0	37.0	39.0	34.0	39.0
11	37.1691074145144	39.0	37.0	39.0	34.0	39.0
12	36.93103671818276	39.0	35.0	39.0	33.0	39.0
13	36.73989342391868	39.0	35.0	39.0	33.0	39.0
14	37.768516837810736	40.0	36.0	41.0	33.0	41.0
15	37.905773519344464	40.0	36.0	41.0	33.0	41.0
16	38.039299389759016	40.0	36.0	41.0	34.0	41.0
17	38.05677899076859	40.0	36.0	41.0	34.0	41.0
18	38.021014849773536	40.0	36.0	41.0	34.0	41.0
19	38.007513484078416	40.0	36.0	41.0	34.0	41.0
20	37.93561136465789	40.0	36.0	41.0	34.0	41.0
21	37.830872056840576	39.0	35.0	41.0	34.0	41.0
22	37.7494640943194	39.0	35.0	41.0	33.0	41.0
23	37.59478507589116	39.0	35.0	41.0	33.0	41.0
24	37.3244671195934	39.0	35.0	41.0	33.0	41.0
25	37.265838389862736	39.0	35.0	41.0	33.0	41.0
26	37.41974812433012	39.0	35.0	41.0	33.0	41.0
27	37.51682160391384	39.0	35.0	41.0	33.0	41.0
28	37.50863823773467	39.0	35.0	41.0	33.0	41.0
29	37.51346463022508	39.0	35.0	41.0	33.0	41.0
30	37.35068436019777	39.0	35.0	41.0	33.0	41.0
31	37.23932402413304	39.0	35.0	41.0	33.0	41.0
32	37.08629378003665	39.0	35.0	41.0	32.0	41.0
33	36.92085559070636	39.0	35.0	41.0	32.0	41.0
34	36.856876447809704	39.0	35.0	41.0	32.0	41.0
35	36.76746879645957	39.0	35.0	41.0	31.0	41.0
36	36.711341320056704	39.0	35.0	41.0	31.0	41.0
37	36.6766057722228	39.0	35.0	41.0	31.0	41.0
38	36.58599665491132	39.0	35.0	41.0	31.0	41.0
39	36.61204426408049	39.0	35.0	41.0	31.0	41.0
40	36.49574624866023	39.0	35.0	41.0	31.0	41.0
41	36.47267313210939	39.0	35.0	41.0	31.0	41.0
42	36.41861256612385	39.0	35.0	41.0	31.0	41.0
43	36.3666070255506	39.0	35.0	41.0	31.0	41.0
44	36.291728252601736	39.0	35.0	40.0	31.0	41.0
45	36.23617233689451	39.0	35.0	40.0	30.0	41.0
46	36.16259875358711	39.0	35.0	40.0	30.0	41.0
47	36.045016077170416	38.0	35.0	40.0	30.0	41.0
48	36.03203008851087	38.0	35.0	40.0	30.0	41.0
49	36.06885955813712	38.0	35.0	40.0	30.0	41.0
50	35.96917461881547	38.0	35.0	40.0	30.0	41.0
51	35.00688898108771	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	6.0
10	11.0
11	10.0
12	15.0
13	6.0
14	26.0
15	21.0
16	49.0
17	103.0
18	247.0
19	410.0
20	739.0
21	1183.0
22	1744.0
23	2575.0
24	4020.0
25	5911.0
26	7836.0
27	9141.0
28	10056.0
29	11249.0
30	14023.0
31	17607.0
32	23583.0
33	33898.0
34	66477.0
35	94398.0
36	68931.0
37	104968.0
38	193284.0
39	252854.0
40	150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.771872731044496	22.341648515022648	24.750630985720708	13.13584776821215
2	32.205770494070464	24.397538291325244	25.95469003906925	17.44200117553504
3	30.383583134529612	24.139957819036752	28.124675863499636	17.351783182933996
4	28.345736956747224	25.074767486083736	28.29819693669398	18.281298620475052
5	24.38424869481036	28.74604553469557	27.210502886975764	19.659202883518308
6	25.67636483075753	33.04161048300661	25.497657573557376	15.78436711267849
7	78.38009542578571	5.357868305500812	10.791368461086332	5.470667807627148
8	77.68709158800954	4.235491650243751	10.97925958579677	7.098157175949936
9	72.56000847076722	6.524651661307609	13.416225841026172	7.499114026899008
10	40.393026311240185	27.091976973343012	19.545863153891364	12.969133561525428
11	29.799705251875668	22.859510597102652	28.07605538844518	19.264728762576496
12	27.913987138263664	20.796381772291948	32.264871382636656	19.024759706807732
13	22.20432268436884	27.109156207862256	32.84442744528576	17.842093662483144
14	16.705887183210592	31.6603568094596	30.472936763129688	21.16081924420012
15	15.396915949244546	25.706725616291532	41.03168326245549	17.864675172008436
16	17.567550046675656	25.397067212944712	36.4653562908412	20.570026449538428
17	18.462058742177508	24.060112194447324	29.6167842201708	27.86104484320437
18	18.924817619195796	26.30065173045673	33.71235694775784	21.062173702589636
19	21.40921584897832	26.881828475607648	29.98230214708018	21.726653528333852
20	24.00198371538222	25.993154237112332	30.460835667116136	19.544026380389308
21	21.267027970819072	26.96686028420288	31.628159250423536	20.137952494554508
22	18.568483559796704	26.833424091553436	28.884451820350588	25.713640528299276
23	18.28281125747675	27.519296926321612	30.27305258790582	23.92483922829582
24	21.236343048784704	23.727548145074852	31.976281851813436	23.059826954327008
25	17.708441378833452	26.741153234450092	30.25933080939045	25.291074577326007
26	17.675703592296788	30.807013449503856	29.578428067627836	21.93885489057152
27	21.279993430833592	28.738698440687344	29.293295992808492	20.688012135670576
28	16.2405352141894	26.944062683677352	32.61342616602704	24.201975936106212
29	20.409146699858244	26.040370120665212	29.743737682812988	23.806745496663556
30	22.51516958821699	27.508276285309268	27.812640459150156	22.163913667323584
31	21.4091078034782	29.00470646198527	26.16689140130692	23.419294333229608
32	24.552043356498288	30.98301956920098	23.65450938699305	20.81042768730768
33	23.463592988279224	28.55566936348235	24.749442485219376	23.23129516301905
34	18.633743041869792	26.912081215641532	29.460226290495456	24.99394945199322
35	19.798041351173808	29.05311084603948	26.770217473982644	24.378630328804064
36	22.25337534142378	27.517568198319676	26.8126793555302	23.416377104726344
37	21.288420979843032	26.477630259654944	30.177324274798607	22.05662448570342
38	20.859588389862736	29.045439615530892	25.003565501503992	25.091406493102376
39	21.501486706081664	26.849738962071708	28.776298274729456	22.872476057117172
40	21.48171437955952	25.661562597240952	30.862548836566056	21.994174186633476
41	17.962780486118316	27.96498461432078	27.302341562078624	26.76989333748228
42	22.173853853334716	24.823453652802268	26.92904435916053	26.073648134702488
43	23.332533796632436	24.204028800608512	26.796148394011688	25.667289008747364
44	21.209115582754208	24.710005877675208	27.45382135324828	26.627057186322308
45	21.675439961276492	24.813189330290772	26.708955675414032	26.802415033018708
46	23.078086643847456	26.56471493275248	28.08016111744978	22.27703730595028
47	18.68808992843066	25.097565086609276	31.485215053763444	24.729129931196624
48	19.48633008332469	22.80667634754348	30.066361546174324	27.640632022957508
49	20.821880510320508	20.707676416692596	32.897369740345056	25.573073332641844
50	20.026881720430108	22.106541506759324	31.01662171973862	26.84995505307195
51	18.193565674376792	23.04513276631055	27.064101234311792	31.697200325000864
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	356.0
1	560.0
2	764.0
3	3455.0
4	6146.0
5	4216.5
6	2287.0
7	2393.5
8	2500.0
9	2576.0
10	2652.0
11	2703.0
12	2754.0
13	2747.5
14	2741.0
15	2710.0
16	2679.0
17	2934.0
18	3189.0
19	2777.5
20	2366.0
21	2394.0
22	2422.0
23	2669.0
24	2916.0
25	3852.0
26	6096.0
27	7404.0
28	8478.5
29	9553.0
30	11338.5
31	13124.0
32	14584.0
33	16044.0
34	17753.0
35	19462.0
36	21133.5
37	22805.0
38	27475.0
39	32145.0
40	36359.0
41	40573.0
42	48473.0
43	56373.0
44	67513.0
45	78653.0
46	90302.5
47	101952.0
48	114740.0
49	127528.0
50	122895.5
51	118263.0
52	96401.0
53	74539.0
54	61121.0
55	47703.0
56	39777.0
57	31851.0
58	28374.5
59	24898.0
60	23184.0
61	21470.0
62	19008.0
63	16546.0
64	13615.0
65	10684.0
66	8877.0
67	7070.0
68	4906.5
69	2743.0
70	2537.0
71	2331.0
72	1884.5
73	1438.0
74	1159.0
75	603.5
76	327.0
77	379.0
78	431.0
79	247.5
80	64.0
81	81.5
82	99.0
83	55.0
84	11.0
85	8.5
86	6.0
87	4.5
88	3.0
89	2.0
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	925536.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.072011775005578
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.06071399778234	15.010815594471044
2	12.541718851820209	5.787253700560106
3	6.045135799855054	4.184203330672907
4	3.656160888231214	3.3741994825834167
5	2.3307254629977545	2.6887262663294758
6	1.6299992770934504	2.25644175086104
7	1.1734611657904617	1.8951876884240513
8	0.8871806565928403	1.637523404437375
9	0.6521620352713241	1.3542021141292433
>10	4.1914152050986715	19.884486007989185
>50	1.3225460022485314	22.306818330563072
>100	0.49885092320182306	15.413776693134619
>500	0.005201289246654079	0.8567209414188478
>1k	0.003782755815748421	1.9725418746713594
>5k	9.456889539371053E-4	1.3771028197542488
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC	6867	0.7419484493309823	TruSeq Adapter, Index 23 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC	5756	0.621909898696539	TruSeq Adapter, Index 23 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGCT	4097	0.44266241399578193	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3449	0.3726489299173668	No Hit
TCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC	2890	0.3122514953497217	TruSeq Adapter, Index 23 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC	2126	0.22970473325726928	TruSeq Adapter, Index 20 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTG	1753	0.1894037617121322	No Hit
GCCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTG	1307	0.14121546865816131	No Hit
ACCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTG	1286	0.1389465131556201	No Hit
AGCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTG	1173	0.12673737164194585	No Hit
GGCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTG	944	0.10199495211423434	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0804550012101096E-4	0.0	0.0	0.46772897002385644	0.0
2	2.160910002420219E-4	0.0	0.0	2.4893683227880925	0.0
3	2.160910002420219E-4	0.0	0.0	3.5362211734605675	0.0
4	2.160910002420219E-4	0.0	0.0	4.658273692217267	0.0
5	2.160910002420219E-4	0.0	0.0	7.455247553849877	0.0
6	2.160910002420219E-4	0.0	0.0	8.82666908688587	0.0
7	2.160910002420219E-4	0.0	0.0	10.37636569512153	0.0
8	2.160910002420219E-4	0.0	0.0	12.753582788784012	0.0
9	2.160910002420219E-4	0.0	0.0	13.556144763682882	0.0
10	2.160910002420219E-4	0.0	0.0	15.37908844172458	0.0
11	2.160910002420219E-4	0.0	0.0	18.118798188293052	0.0
12	2.160910002420219E-4	0.0	0.0	20.204724613629292	0.0
13	2.160910002420219E-4	0.0	0.0	21.027923279051276	0.0
14	2.160910002420219E-4	0.0	0.0	21.309381806866508	0.0
15	2.160910002420219E-4	0.0	0.0	21.904172285032672	0.0
16	2.160910002420219E-4	0.0	0.0	23.147992082425752	0.0
17	2.160910002420219E-4	0.0	0.0	24.733235660201224	0.0
18	2.160910002420219E-4	0.0	0.0	26.532085191715936	0.0
19	2.160910002420219E-4	0.0	0.0	27.5202693358227	0.0
20	2.160910002420219E-4	0.0	0.0	28.42644694533762	0.0
21	2.160910002420219E-4	0.0	0.0	29.547851191093592	0.0
22	2.160910002420219E-4	0.0	0.0	30.708043771393008	0.0
23	2.160910002420219E-4	0.0	0.0	31.848139888669916	0.0
24	2.160910002420219E-4	0.0	0.0	32.64335476956056	0.0
25	2.160910002420219E-4	0.0	0.0	33.35818379836117	0.0
26	2.160910002420219E-4	0.0	0.0	34.03249576461639	0.0
27	2.160910002420219E-4	0.0	0.0	34.646518341804104	0.0
28	2.160910002420219E-4	0.0	0.0	35.32007398955848	0.0
29	2.160910002420219E-4	0.0	0.0	36.022693876845416	0.0
30	3.241365003630329E-4	0.0	0.0	36.78614338070048	0.0
31	3.241365003630329E-4	0.0	0.0	37.536087197040416	0.0
32	3.241365003630329E-4	0.0	0.0	38.18371192476576	0.0
33	3.241365003630329E-4	0.0	0.0	38.809727552466896	0.0
34	3.241365003630329E-4	0.0	0.0	39.4664281022024	0.0
35	3.241365003630329E-4	0.0	0.0	40.12528956194033	0.0
36	3.241365003630329E-4	0.0	0.0	40.824343947723264	0.0
37	3.241365003630329E-4	0.0	0.0	41.461488261936864	0.0
38	0.0011885005013311206	0.0	0.0	42.06114078760848	0.0
39	0.0012965460014521316	0.0	0.0	42.661549631780936	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATAA	20	7.032573E-4	45.000004	10
AACGTAG	40	6.8121153E-9	45.000004	1
CGGAACA	20	7.032573E-4	45.000004	17
TCGCACC	20	7.032573E-4	45.000004	23
CAAACGA	20	7.032573E-4	45.000004	19
ACCGGAT	20	7.032573E-4	45.000004	24
CGAACAA	20	7.032573E-4	45.000004	12
ACGCCTA	35	1.2115925E-7	45.000004	18
CTCGTTA	20	7.032573E-4	45.000004	20
GAATGCG	35	1.2115925E-7	45.000004	1
CGCGGAG	20	7.032573E-4	45.000004	43
CGACGCC	20	7.032573E-4	45.000004	22
CTATCGA	20	7.032573E-4	45.000004	19
CGGCCGT	45	3.8380676E-10	45.000004	17
TAGCGTA	20	7.032573E-4	45.000004	15
CACATCG	20	7.032573E-4	45.000004	38
GGCCGTC	20	7.032573E-4	45.000004	19
CTCCCCG	35	1.2115925E-7	45.000004	13
CGCGCCG	20	7.032573E-4	45.000004	23
CGTCTCG	20	7.032573E-4	45.000004	37
>>END_MODULE