SRR2934759.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934759.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	689683
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC	6619	0.9597162754482855	Illumina PCR Primer Index 9 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC	4758	0.6898821632547127	RNA PCR Primer, Index 17 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGC	4061	0.5888212410629231	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCG	3631	0.5264737567839138	No Hit
CTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGCT	3508	0.5086394763971274	RNA PCR Primer, Index 17 (96% over 25bp)
GAATCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTC	3399	0.4928351141031459	RNA PCR Primer, Index 17 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2795	0.4052586478135607	No Hit
TCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC	2091	0.3031827665753687	Illumina PCR Primer Index 9 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC	1563	0.22662585564672466	Illumina PCR Primer Index 9 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTG	1498	0.21720123593013024	RNA PCR Primer, Index 17 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTG	1045	0.1515188862129413	Illumina PCR Primer Index 9 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTG	937	0.13585951806844593	Illumina PCR Primer Index 9 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTG	882	0.1278848398467122	RNA PCR Primer, Index 17 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCGAGATAT	842	0.12208507386726945	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCT	800	0.11599531958885459	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTC	744	0.10787564721763476	No Hit
GGCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTG	713	0.10338082858356665	RNA PCR Primer, Index 17 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCC	695	0.10077093389281742	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAT	20	7.031321E-4	45.000004	16
GTGCATG	50	2.1827873E-11	45.000004	1
CGGGTTC	65	0.0	45.000004	6
GTTTGCG	20	7.031321E-4	45.000004	1
TTAGGTT	20	7.031321E-4	45.000004	25
AACGTAG	20	7.031321E-4	45.000004	1
TTAGGCG	25	3.889176E-5	45.000004	1
AACCGGT	25	3.889176E-5	45.000004	14
AACCGGC	25	3.889176E-5	45.000004	12
TCGTTAA	25	3.889176E-5	45.000004	41
GTTCTCG	20	7.031321E-4	45.000004	11
GATCGCG	25	3.889176E-5	45.000004	9
ACGTTAG	25	3.889176E-5	45.000004	1
TTAGCAA	25	3.889176E-5	45.000004	36
TAGACGG	30	2.1642336E-6	45.000004	2
ACCGCCC	20	7.031321E-4	45.000004	45
CATGACG	20	7.031321E-4	45.000004	1
CGGTTGC	20	7.031321E-4	45.000004	43
GACGTAA	30	2.1642336E-6	45.000004	45
CGGTTAG	25	3.889176E-5	45.000004	5

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content