##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934759.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	689683
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.953155290184043	33.0	31.0	34.0	30.0	34.0
2	32.13677298121021	33.0	31.0	34.0	30.0	34.0
3	32.138459263168734	34.0	31.0	34.0	30.0	34.0
4	35.75432191311081	37.0	35.0	37.0	35.0	37.0
5	35.632747218649726	37.0	35.0	37.0	33.0	37.0
6	35.544461730969154	37.0	35.0	37.0	33.0	37.0
7	35.92375192660976	37.0	35.0	37.0	35.0	37.0
8	35.69401594645656	37.0	35.0	37.0	35.0	37.0
9	37.460395573038625	39.0	37.0	39.0	35.0	39.0
10	37.1873034423061	39.0	37.0	39.0	34.0	39.0
11	37.04261813035844	39.0	37.0	39.0	33.0	39.0
12	36.723573003829294	39.0	35.0	39.0	33.0	39.0
13	36.42918993218623	39.0	35.0	39.0	32.0	39.0
14	37.39945018218515	39.0	35.0	41.0	33.0	41.0
15	37.52208188399598	39.0	35.0	41.0	33.0	41.0
16	37.72211581262696	39.0	35.0	41.0	33.0	41.0
17	37.71827926743156	39.0	35.0	41.0	33.0	41.0
18	37.7173919032367	39.0	36.0	41.0	33.0	41.0
19	37.65116582545894	39.0	36.0	41.0	33.0	41.0
20	37.53037844922957	39.0	35.0	41.0	33.0	41.0
21	37.461588874888896	39.0	35.0	41.0	33.0	41.0
22	37.3781621411576	39.0	35.0	41.0	33.0	41.0
23	37.19856948772117	39.0	35.0	40.0	33.0	41.0
24	36.93558489914932	39.0	35.0	40.0	32.0	41.0
25	36.86678952504266	38.0	35.0	40.0	32.0	41.0
26	37.07424860406883	39.0	35.0	40.0	33.0	41.0
27	37.14903658637374	39.0	35.0	40.0	33.0	41.0
28	37.10997806238518	39.0	35.0	40.0	33.0	41.0
29	37.09391416056362	39.0	35.0	40.0	33.0	41.0
30	36.9584142279859	39.0	35.0	40.0	32.0	41.0
31	36.76471219386298	39.0	35.0	40.0	31.0	41.0
32	36.56962546561246	38.0	35.0	40.0	31.0	41.0
33	36.439423619256964	38.0	35.0	40.0	31.0	41.0
34	36.31378763866878	38.0	35.0	40.0	31.0	41.0
35	36.197360236514456	38.0	35.0	40.0	30.0	41.0
36	36.16275158297363	38.0	35.0	40.0	30.0	41.0
37	35.98220776791656	38.0	35.0	40.0	30.0	41.0
38	35.86272533903257	38.0	35.0	40.0	30.0	41.0
39	35.86054752690729	38.0	35.0	40.0	29.0	41.0
40	35.82010575873264	38.0	35.0	40.0	29.0	41.0
41	35.78019031932062	38.0	35.0	40.0	29.0	41.0
42	35.751709118537065	38.0	35.0	40.0	29.0	41.0
43	35.66900010584573	38.0	35.0	40.0	29.0	41.0
44	35.55072402828546	38.0	35.0	40.0	29.0	41.0
45	35.45366494461948	38.0	35.0	40.0	28.0	41.0
46	35.38648770522109	38.0	34.0	40.0	28.0	41.0
47	35.192353588532704	37.0	34.0	40.0	28.0	41.0
48	35.19377018137318	37.0	34.0	40.0	28.0	41.0
49	35.18967264670871	37.0	34.0	40.0	28.0	41.0
50	34.94716123204429	36.0	34.0	39.0	28.0	41.0
51	33.87567041669869	36.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	9.0
11	6.0
12	12.0
13	10.0
14	14.0
15	21.0
16	55.0
17	95.0
18	238.0
19	454.0
20	739.0
21	1249.0
22	1817.0
23	2728.0
24	3872.0
25	5305.0
26	6937.0
27	8039.0
28	8955.0
29	10321.0
30	12306.0
31	15914.0
32	20794.0
33	29649.0
34	56312.0
35	79521.0
36	57062.0
37	85452.0
38	141505.0
39	140216.0
40	70.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.890984698767404	21.408096183319003	24.003926441568083	12.69699267634551
2	31.390798381285318	25.42892894271716	24.903035162531193	18.277237513466332
3	29.72713551008217	25.690788376689	28.639389400637683	15.942686712591147
4	26.469551953578673	26.085897434038536	29.14962381267916	18.29492679970363
5	25.88928536733543	27.239470887349693	27.33038221907746	19.540861526237414
6	23.61302221455364	33.72288428161924	26.62049086319367	16.043602640633452
7	74.49233923411191	5.784831582045665	13.635249817669855	6.087579366172575
8	73.12388445126238	6.535176305636067	12.181248486623565	8.159690756477977
9	68.80088388433526	5.654771829956661	15.012259255339048	10.532085030369025
10	42.65060904792492	21.65864607363093	21.181180339373306	14.50956453907085
11	33.59934926625711	22.175115234100303	24.26361096329763	19.96192453634496
12	28.719426171153994	20.53610136830979	30.860409782465275	19.88406267807094
13	21.806250117807746	28.724210978087033	33.52409730267384	15.945441601431382
14	18.048001763128855	32.9947236629002	28.842526204067664	20.114748369903275
15	14.650498852371307	24.632476079590187	42.19141257650254	18.525612491535966
16	15.68372716160903	24.326538424174586	36.16386078821719	23.82587362599919
17	16.361719804605883	24.134421176105544	29.5935958984055	29.910263120883073
18	19.90175196430824	24.61696170559518	33.73984859710911	21.741437732987475
19	20.598158864289825	26.275549781566315	28.533253683213882	24.59303767092998
20	23.64231103274983	26.60106164716254	28.930827641104685	20.825799678982953
21	21.128112480661404	28.56979220888437	29.639849032091554	20.662246278362666
22	17.954045554261885	25.798664023906635	27.34198175103635	28.905308670795133
23	17.19920601203741	28.373325136330752	28.56979220888437	25.857676642747464
24	20.559590420526533	24.013786043733134	30.741369585737218	24.685253950003116
25	17.522542965391345	27.041843861600185	29.164848198375193	26.270764974633277
26	18.737013961486653	27.61080090418352	29.501379619332358	24.15080551499747
27	21.50480728102621	27.422598498150602	27.084762129848063	23.98783209097513
28	18.002908582638693	25.895955098211786	31.751253836907683	24.349882482241842
29	22.206288976239808	23.6468058513839	29.718000878664547	24.42890429371175
30	24.308414155488826	25.46619243913508	25.938873366459664	24.28652003891643
31	27.207282186163788	27.77594924044815	21.839453778040056	23.177314795348007
32	26.281494541695245	29.43671222866157	22.764081469312714	21.51771176033047
33	25.21926740256031	27.60253623766281	22.291690530287102	24.88650582948978
34	23.43351945748989	27.126665439049535	26.4984057893264	22.941409314134177
35	20.9210608351953	26.474046772212738	27.351696359051914	25.25319603354005
36	27.4298482056249	27.068087802657164	24.785299913148503	20.71676407856943
37	22.07564924755286	29.183697437808387	28.25979471728316	20.480858597355596
38	21.720268587162508	30.685691832334566	25.355996885525666	22.23804269497726
39	23.549369782929258	30.09803054446753	26.395024960742834	19.957574711860378
40	24.94725837812444	24.1109321238888	29.263734208324692	21.678075289662065
41	19.24202858414663	27.247590559720912	26.26598016770023	27.244400688432226
42	23.371316967360368	23.611137290610323	28.256749840143954	24.76079590188536
43	25.048029312017263	23.207908560889567	26.675588639998377	25.068473487094796
44	22.996072108490424	25.447343199701894	26.2819295241437	25.274655167663983
45	22.032440991006013	24.61029197471882	25.62771592166256	27.729551112612604
46	25.933943565377138	25.04643437637291	27.103756363430737	21.915865694819214
47	19.01598270509785	25.234781776555316	31.78199259659873	23.96724292174811
48	20.626142735140636	22.878336859107733	30.264048845629077	26.23147156012255
49	22.205998987940838	20.14621210034175	33.61051381576753	24.037275095949877
50	21.547435560975114	21.47102364419596	31.072826211462367	25.90871458336656
51	19.779521896291484	21.56976465999597	26.475351719558116	32.17536172415443
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	462.0
1	526.5
2	591.0
3	2663.0
4	4735.0
5	3245.5
6	1756.0
7	1883.0
8	2010.0
9	2059.5
10	2109.0
11	2276.5
12	2444.0
13	2367.0
14	2290.0
15	2269.0
16	2248.0
17	2270.5
18	2293.0
19	2151.5
20	2010.0
21	1900.5
22	1791.0
23	1902.5
24	2014.0
25	2157.0
26	2771.5
27	3243.0
28	3325.5
29	3408.0
30	4145.5
31	4883.0
32	5971.5
33	7060.0
34	8499.5
35	9939.0
36	11051.5
37	12164.0
38	13650.5
39	15137.0
40	19911.0
41	24685.0
42	32026.5
43	39368.0
44	47667.0
45	55966.0
46	71634.5
47	87303.0
48	93133.5
49	98964.0
50	97536.5
51	96109.0
52	78964.5
53	61820.0
54	51441.5
55	41063.0
56	34931.5
57	28800.0
58	24736.5
59	20673.0
60	17864.5
61	15056.0
62	13760.5
63	12465.0
64	10357.0
65	8249.0
66	7026.0
67	5803.0
68	4772.5
69	3742.0
70	2712.5
71	1683.0
72	1375.0
73	1067.0
74	971.0
75	727.5
76	580.0
77	401.5
78	223.0
79	197.0
80	171.0
81	140.0
82	109.0
83	61.0
84	13.0
85	9.0
86	5.0
87	3.5
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	689683.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.233035168118008
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.31890684982135	19.853161700746206
2	11.727381067848397	6.621991242369702
3	5.329280924659092	4.513853273000419
4	3.0326279602984427	3.4248116741969565
5	1.898880998312132	2.6805587002708724
6	1.2439835406699282	2.1072858631376423
7	0.8730260195458946	1.7253722018764923
8	0.6337767749430328	1.4314753580562443
9	0.5189998575104846	1.3187647106407572
>10	3.1191531155765464	17.872375611057205
>50	0.9871902282748862	20.102230251944235
>100	0.305386052759313	12.092576343026783
>500	0.00570330489024184	1.1368125492373227
>1k	0.005184822627492582	4.149819592882934
>5k	5.184822627492581E-4	0.9689109275562503
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC	6619	0.9597162754482855	Illumina PCR Primer Index 9 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC	4758	0.6898821632547127	RNA PCR Primer, Index 17 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGC	4061	0.5888212410629231	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCG	3631	0.5264737567839138	No Hit
CTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGCT	3508	0.5086394763971274	RNA PCR Primer, Index 17 (96% over 25bp)
GAATCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTC	3399	0.4928351141031459	RNA PCR Primer, Index 17 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2795	0.4052586478135607	No Hit
TCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC	2091	0.3031827665753687	Illumina PCR Primer Index 9 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC	1563	0.22662585564672466	Illumina PCR Primer Index 9 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTG	1498	0.21720123593013024	RNA PCR Primer, Index 17 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTG	1045	0.1515188862129413	Illumina PCR Primer Index 9 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTG	937	0.13585951806844593	Illumina PCR Primer Index 9 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTG	882	0.1278848398467122	RNA PCR Primer, Index 17 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCGAGATAT	842	0.12208507386726945	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCT	800	0.11599531958885459	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTC	744	0.10787564721763476	No Hit
GGCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTG	713	0.10338082858356665	RNA PCR Primer, Index 17 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCC	695	0.10077093389281742	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.349824484582047E-4	0.0	0.0	0.5393782360881738	0.0
2	4.349824484582047E-4	0.0	0.0	2.856384744875544	0.0
3	4.349824484582047E-4	0.0	0.0	3.9726947017687837	0.0
4	4.349824484582047E-4	0.0	0.0	5.17614614250315	0.0
5	4.349824484582047E-4	0.0	0.0	8.623382046534422	0.0
6	4.349824484582047E-4	0.0	0.0	10.265730777763117	0.0
7	4.349824484582047E-4	0.0	0.0	12.161964264741918	0.0
8	4.349824484582047E-4	0.0	0.0	15.056192482633326	0.0
9	4.349824484582047E-4	0.0	0.0	16.07057155243786	0.0
10	4.349824484582047E-4	0.0	0.0	18.68365611447578	0.0
11	4.349824484582047E-4	0.0	0.0	21.657341126285555	0.0
12	4.349824484582047E-4	0.0	0.0	24.504301251444502	0.0
13	4.349824484582047E-4	0.0	0.0	25.429073936866647	0.0
14	4.349824484582047E-4	0.0	0.0	25.743421252952444	0.0
15	4.349824484582047E-4	0.0	0.0	26.415904118268827	0.0
16	4.349824484582047E-4	0.0	0.0	27.79653840967517	0.0
17	4.349824484582047E-4	0.0	0.0	29.401913632784918	0.0
18	4.349824484582047E-4	0.0	0.0	31.321926160279432	0.0
19	4.349824484582047E-4	0.0	0.0	32.39879770851246	0.0
20	5.799765979442729E-4	0.0	0.0	33.29645648798071	0.0
21	5.799765979442729E-4	0.0	0.0	34.353028855285686	0.0
22	5.799765979442729E-4	0.0	0.0	35.387272123569815	0.0
23	5.799765979442729E-4	0.0	0.0	36.47632318035967	0.0
24	5.799765979442729E-4	0.0	0.0	37.292930230265206	0.0
25	5.799765979442729E-4	0.0	0.0	37.97512770359716	0.0
26	5.799765979442729E-4	0.0	0.0	38.677044381259215	0.0
27	5.799765979442729E-4	0.0	0.0	39.29384949317295	0.0
28	5.799765979442729E-4	0.0	0.0	39.94458323606642	0.0
29	5.799765979442729E-4	0.0	0.0	40.58922722468149	0.0
30	5.799765979442729E-4	0.0	0.0	41.378140392035185	0.0
31	5.799765979442729E-4	0.0	0.0	42.08527685907874	0.0
32	5.799765979442729E-4	0.0	0.0	42.68787254434284	0.0
33	5.799765979442729E-4	0.0	0.0	43.27683877955525	0.0
34	5.799765979442729E-4	0.0	0.0	43.903793481933	0.0
35	5.799765979442729E-4	0.0	0.0	44.59034077974954	0.0
36	5.799765979442729E-4	0.0	0.0	45.18655672243625	0.0
37	5.799765979442729E-4	0.0	0.0	45.736954513885365	0.0
38	5.799765979442729E-4	0.0	0.0	46.31780107672655	0.0
39	5.799765979442729E-4	0.0	0.0	46.9105371598256	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAT	20	7.031321E-4	45.000004	16
GTGCATG	50	2.1827873E-11	45.000004	1
CGGGTTC	65	0.0	45.000004	6
GTTTGCG	20	7.031321E-4	45.000004	1
TTAGGTT	20	7.031321E-4	45.000004	25
AACGTAG	20	7.031321E-4	45.000004	1
TTAGGCG	25	3.889176E-5	45.000004	1
AACCGGT	25	3.889176E-5	45.000004	14
AACCGGC	25	3.889176E-5	45.000004	12
TCGTTAA	25	3.889176E-5	45.000004	41
GTTCTCG	20	7.031321E-4	45.000004	11
GATCGCG	25	3.889176E-5	45.000004	9
ACGTTAG	25	3.889176E-5	45.000004	1
TTAGCAA	25	3.889176E-5	45.000004	36
TAGACGG	30	2.1642336E-6	45.000004	2
ACCGCCC	20	7.031321E-4	45.000004	45
CATGACG	20	7.031321E-4	45.000004	1
CGGTTGC	20	7.031321E-4	45.000004	43
GACGTAA	30	2.1642336E-6	45.000004	45
CGGTTAG	25	3.889176E-5	45.000004	5
>>END_MODULE