##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934758.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2401581
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00569000171137	33.0	31.0	34.0	30.0	34.0
2	32.20980012749934	34.0	31.0	34.0	30.0	34.0
3	32.173469476982035	34.0	31.0	34.0	30.0	34.0
4	35.79756668627875	37.0	35.0	37.0	35.0	37.0
5	35.681797532542106	37.0	35.0	37.0	33.0	37.0
6	35.62640527219361	37.0	35.0	37.0	33.0	37.0
7	36.00212318468542	37.0	35.0	37.0	35.0	37.0
8	35.88384734889225	37.0	35.0	37.0	35.0	37.0
9	37.596081497979874	39.0	38.0	39.0	35.0	39.0
10	37.223694724433614	39.0	37.0	39.0	34.0	39.0
11	37.13163037182589	39.0	37.0	39.0	34.0	39.0
12	37.12411615514946	39.0	37.0	39.0	34.0	39.0
13	37.109571569728445	39.0	37.0	39.0	33.0	39.0
14	38.238445424076886	40.0	38.0	41.0	33.0	41.0
15	38.285933308099956	40.0	38.0	41.0	34.0	41.0
16	38.35569193793589	40.0	38.0	41.0	34.0	41.0
17	38.3556511314838	40.0	38.0	41.0	34.0	41.0
18	38.29781881185769	40.0	38.0	41.0	34.0	41.0
19	38.21363093728673	40.0	37.0	41.0	34.0	41.0
20	38.12331418344832	40.0	37.0	41.0	34.0	41.0
21	38.07175648041852	40.0	37.0	41.0	34.0	41.0
22	38.00743635130358	40.0	36.0	41.0	34.0	41.0
23	37.82004188074439	39.0	36.0	41.0	33.0	41.0
24	37.5635483458605	39.0	36.0	41.0	33.0	41.0
25	37.498223461961096	39.0	36.0	41.0	33.0	41.0
26	37.685947715275894	39.0	36.0	41.0	33.0	41.0
27	37.76930988378073	39.0	36.0	41.0	33.0	41.0
28	37.69459493558618	39.0	36.0	41.0	33.0	41.0
29	37.68876211129252	40.0	36.0	41.0	33.0	41.0
30	37.52656729046407	39.0	36.0	41.0	33.0	41.0
31	37.353623717043064	39.0	36.0	41.0	33.0	41.0
32	37.17050184857392	39.0	35.0	41.0	32.0	41.0
33	36.9253329369278	39.0	35.0	41.0	31.0	41.0
34	36.7499921926431	39.0	35.0	41.0	31.0	41.0
35	36.66693149221284	39.0	35.0	41.0	31.0	41.0
36	36.55552113378645	39.0	35.0	41.0	30.0	41.0
37	36.35358124502151	39.0	35.0	41.0	30.0	41.0
38	36.22374718987201	39.0	35.0	41.0	30.0	41.0
39	36.27835954731487	39.0	35.0	41.0	30.0	41.0
40	36.26009699443825	39.0	35.0	41.0	30.0	41.0
41	36.27445212133174	39.0	35.0	41.0	30.0	41.0
42	36.117135337096684	39.0	35.0	41.0	29.0	41.0
43	35.97891222490518	39.0	35.0	40.0	28.0	41.0
44	35.900778278975395	39.0	35.0	40.0	28.0	41.0
45	35.897738614687576	39.0	35.0	40.0	28.0	41.0
46	35.885567049372895	39.0	35.0	40.0	28.0	41.0
47	35.747608346335184	39.0	35.0	40.0	28.0	41.0
48	35.67762611379754	39.0	35.0	40.0	28.0	41.0
49	35.60065473535975	38.0	35.0	40.0	28.0	41.0
50	35.388331686501516	38.0	35.0	40.0	27.0	41.0
51	34.38521832076453	37.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	24.0
10	23.0
11	21.0
12	25.0
13	21.0
14	31.0
15	44.0
16	109.0
17	255.0
18	498.0
19	980.0
20	1724.0
21	3051.0
22	4793.0
23	7473.0
24	12006.0
25	19536.0
26	27611.0
27	30276.0
28	30591.0
29	32523.0
30	37978.0
31	47100.0
32	60061.0
33	81620.0
34	132751.0
35	182257.0
36	200414.0
37	313415.0
38	552308.0
39	621698.0
40	356.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.69378630160715	22.274868097307564	25.760446972223715	15.27089862886157
2	34.46125698029756	23.915037635624202	25.021433797152792	16.602271586925447
3	28.32096856195981	23.10990135248405	32.68838319423747	15.880746891318678
4	25.921091147873003	25.049540282005893	31.95428344911123	17.075085121009867
5	23.520297670576173	28.48552682587012	29.785129046240787	18.209046457312912
6	24.187691358317707	32.39311936595101	28.2323602660081	15.18682900972318
7	76.25330979883668	5.1542296512172605	12.711251463098684	5.881209086847373
8	77.00090065669241	3.422162317240185	12.762634281333836	6.814302744733573
9	71.5827198832769	5.410644071551199	15.270107483362002	7.736528561809908
10	35.396099486130176	25.279014116117676	23.576010969440546	15.748875428311598
11	23.394338979197453	24.65409244993194	30.43628343162275	21.51528513924785
12	21.02427525867335	21.861973425006276	35.61149925819699	21.50225205812338
13	20.686789244252015	24.662545215006283	36.862675046146684	17.787990494595018
14	18.376269632379667	28.301481399128324	32.96253592945647	20.359713039035533
15	16.78764946924547	26.564042603601546	37.18000766994742	19.468300257205566
16	18.953805847064913	26.701535363579243	33.25896565637386	21.08569313298198
17	19.05519738872018	26.068868799345097	30.631529813068976	24.244403998865746
18	19.861832684385828	25.793591804731964	34.056190484518325	20.288385026363883
19	19.203224875613188	28.3031469686011	31.203194895362678	21.29043326042303
20	19.704103255313896	28.118643510254287	31.233966291372223	20.943286943059594
21	18.99357131822745	28.549484693624745	32.775742313084585	19.68120167506322
22	18.179690795355228	26.259451586267545	30.250572435408174	25.31028518296905
23	17.210329362199317	27.653533234981452	30.643355356325685	24.492782046493538
24	19.22317007004969	25.25936039633891	32.09977094255826	23.417698591053142
25	18.55685900246546	27.920107629099334	29.568688293253487	23.954345075181724
26	19.373737550388682	27.7368533478571	30.70481486987114	22.184594231883082
27	21.90136414303744	26.851561533839586	29.86940686156328	21.377667461559696
28	18.923492482660382	27.001462786389467	30.855257432499673	23.219787298450477
29	21.127915319116866	25.839769718364693	29.284125748829627	23.748189213688818
30	23.478700072993583	28.090245550743447	27.64199916638248	20.78905520988049
31	21.47739343374219	30.68203820733092	26.44649503806035	21.394073320866546
32	23.43127298225627	30.515023228448257	25.464725112332253	20.58897867696322
33	23.329756522890545	29.773470059931356	25.729467380030073	21.16730603714803
34	20.94511906947965	28.8469137622258	28.361025507780084	21.846941660514467
35	24.211217527120677	27.580039981995192	26.806299683416885	21.40244280746725
36	27.00750047572828	28.334626231636577	25.800545557280806	18.857327735354335
37	22.684306712952843	29.164371303736996	29.708096458124878	18.443225525185284
38	21.69395910443995	31.241836107131093	26.593939575637883	20.470265212791077
39	22.781867444820726	28.255844795574248	28.549984364466575	20.412303395138455
40	23.788579273403645	24.680824839970004	31.149105526734267	20.381490359892087
41	25.08526674719695	24.660962924007144	27.356395641038134	22.897374687757775
42	26.919433489855226	24.264599028723165	27.57396065341956	21.24200682800205
43	25.20064907242354	25.660970835462138	28.09266062647897	21.045719465635347
44	21.68704699112793	27.529906340864624	26.690709162006193	24.092337506001254
45	22.038815263778318	25.746247992468298	27.450292120065907	24.764644623687477
46	24.919001274577038	25.331188079852396	28.202130180077205	21.547680465493357
47	21.816545017636297	25.08514182948649	31.009905558046967	22.08840759483024
48	22.510462899231797	24.00635248196917	29.3679455325471	24.115239086251933
49	23.99631742589569	22.269788110415597	30.940867703400386	22.793026760288328
50	21.65735821527569	22.066088963895034	31.49908331220142	24.77746950862786
51	21.186418446848137	21.65019626654275	28.10007241063283	29.063312875976283
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	526.0
1	839.5
2	1153.0
3	12767.0
4	24381.0
5	16599.0
6	8817.0
7	9038.5
8	9260.0
9	9727.0
10	10194.0
11	10589.5
12	10985.0
13	11093.5
14	11202.0
15	10971.5
16	10741.0
17	10362.5
18	9984.0
19	9572.5
20	9161.0
21	8662.0
22	8163.0
23	8818.0
24	9473.0
25	10638.0
26	13707.0
27	15611.0
28	18281.0
29	20951.0
30	24455.0
31	27959.0
32	33245.0
33	38531.0
34	44076.0
35	49621.0
36	55572.5
37	61524.0
38	72167.0
39	82810.0
40	93929.5
41	105049.0
42	123867.5
43	142686.0
44	167170.0
45	191654.0
46	239627.0
47	287600.0
48	323374.0
49	359148.0
50	343411.0
51	327674.0
52	262420.0
53	197166.0
54	160199.0
55	123232.0
56	100680.5
57	78129.0
58	66249.5
59	54370.0
60	45391.0
61	36412.0
62	30521.5
63	24631.0
64	20967.0
65	17303.0
66	13129.5
67	8956.0
68	7152.5
69	5349.0
70	4482.5
71	3616.0
72	3061.5
73	2507.0
74	1873.5
75	1066.5
76	893.0
77	634.0
78	375.0
79	428.0
80	481.0
81	328.0
82	175.0
83	108.5
84	42.0
85	30.0
86	18.0
87	14.5
88	11.0
89	10.5
90	10.0
91	6.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2401581.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.76643127222724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.24487392413529	20.31406349344586
2	12.975207847553499	7.7245126527412955
3	5.683104086550237	5.074971816156343
4	3.056836609816158	3.6396446742608313
5	1.8948296053894131	2.8201157610702716
6	1.2285082511247634	2.1940983854681613
7	0.8630096299722029	1.7982101786486488
8	0.6658889374476693	1.5856909833177977
9	0.498623206631357	1.3358010069836865
>10	4.0613794707361	26.088043867914912
>50	0.6833729816120871	13.916003035999957
>100	0.13336000429092526	6.335485486588177
>500	0.006913676824007432	1.464759871518522
>1k	0.0028219089077581357	1.695418812176273
>5k	5.643817815516271E-4	1.2603083560192627
>10k+	7.054772269395339E-4	2.7528716176899026
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15804	0.6580664986939854	No Hit
CCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGC	15379	0.6403698230457353	No Hit
CGCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTG	11870	0.49425774104641895	No Hit
GCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGC	11580	0.4821823623687896	No Hit
CTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGCT	10913	0.4544089914102418	Illumina Single End Adapter 2 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTC	9705	0.4041087933323923	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTT	8696	0.3620948033816057	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCT	6356	0.2646589892241819	No Hit
TCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGC	5251	0.2186476325387318	No Hit
CGTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCT	4496	0.18721000874007582	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCC	4252	0.1770500349561393	No Hit
ACTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGC	3834	0.15964483396562512	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTC	3039	0.12654164069419271	No Hit
ACCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTG	2476	0.10309875036486381	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.163923681941188E-5	0.0	0.0	0.4791843373177919	0.0
2	4.163923681941188E-5	0.0	0.0	2.073134322764879	0.0
3	4.163923681941188E-5	0.0	0.0	3.249109649018709	0.0
4	4.163923681941188E-5	0.0	0.0	4.326025230879158	0.0
5	4.163923681941188E-5	0.0	0.0	7.118060977331183	0.0
6	4.163923681941188E-5	0.0	0.0	9.036672092259224	0.0
7	4.163923681941188E-5	0.0	0.0	10.907064970950387	0.0
8	4.163923681941188E-5	0.0	0.0	13.638848741724722	0.0
9	4.163923681941188E-5	0.0	0.0	14.740872783387276	0.0
10	1.2491771045823564E-4	0.0	0.0	16.886834131349307	0.0
11	1.2491771045823564E-4	0.0	0.0	19.891063428633053	0.0
12	1.2491771045823564E-4	0.0	0.0	22.092446600801722	0.0
13	1.2491771045823564E-4	0.0	0.0	22.980653161396596	0.0
14	1.2491771045823564E-4	0.0	0.0	23.323927029735827	0.0
15	1.2491771045823564E-4	0.0	0.0	23.906293395892124	0.0
16	1.2491771045823564E-4	0.0	0.0	25.196776623399337	0.0
17	1.2491771045823564E-4	0.0	0.0	26.869174931014193	0.0
18	1.665569472776475E-4	0.0	0.0	28.617731402771756	0.0
19	2.0819618409705938E-4	0.0	0.0	29.679698498614037	0.0
20	2.0819618409705938E-4	0.0	0.0	30.629406211991185	0.0
21	2.0819618409705938E-4	0.0	0.0	31.816707410659895	0.0
22	2.0819618409705938E-4	0.0	0.0	33.051560617776374	0.0
23	2.0819618409705938E-4	0.0	0.0	34.34029499733717	0.0
24	2.0819618409705938E-4	0.0	0.0	35.3333075170065	0.0
25	2.0819618409705938E-4	0.0	0.0	36.19719676329884	0.0
26	2.0819618409705938E-4	0.0	0.0	36.99346388899646	0.0
27	2.0819618409705938E-4	0.0	0.0	37.78877331224722	0.0
28	2.0819618409705938E-4	0.0	0.0	38.580335204184244	0.0
29	2.0819618409705938E-4	0.0	0.0	39.40037833410574	0.0
30	2.0819618409705938E-4	0.0	0.0	40.342382788671294	0.0
31	2.0819618409705938E-4	0.0	0.0	41.16421640577603	0.0
32	2.0819618409705938E-4	0.0	0.0	41.94620127324458	0.0
33	2.0819618409705938E-4	0.0	0.0	42.68867050497152	0.0
34	2.0819618409705938E-4	0.0	0.0	43.4192309149681	0.0
35	2.0819618409705938E-4	0.0	0.0	44.17927190463282	0.0
36	2.0819618409705938E-4	0.0	0.0	44.89325989837528	0.0
37	2.0819618409705938E-4	0.0	0.0	45.614409840850676	0.0
38	2.0819618409705938E-4	0.0	0.0	46.32223522754386	0.0
39	2.0819618409705938E-4	0.0	0.0	46.99579152233466	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATCCG	25	3.8920793E-5	45.000004	27
TCACCCG	20	7.034824E-4	45.0	12
ACGTTCG	20	7.034824E-4	45.0	1
CGCCTAG	20	7.034824E-4	45.0	37
TCGTCGA	20	7.034824E-4	45.0	24
GACGGTC	75	0.0	45.0	29
CGTCGAC	20	7.034824E-4	45.0	25
CGTTTTT	11340	0.0	43.92857	1
TGTTACG	125	0.0	43.199997	1
CGTTTTC	755	0.0	42.615894	1
TATCGAG	125	0.0	41.399998	1
ACGCGAG	60	3.6379788E-12	41.249996	1
AACGTTC	60	3.6379788E-12	41.249996	26
CGTTCTG	1120	0.0	40.982143	1
AAGTACG	165	0.0	40.909092	1
TCGTTAG	55	6.184564E-11	40.909092	1
CGTTTCT	1050	0.0	39.428574	1
CTCGATC	140	0.0	38.57143	16
ACCAACG	35	6.2511317E-6	38.57143	1
CCAACGT	35	6.2511317E-6	38.57143	17
>>END_MODULE