##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934757.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	874842
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.93790764503762	33.0	31.0	34.0	30.0	34.0
2	32.12596903212237	33.0	31.0	34.0	30.0	34.0
3	32.13200440765304	34.0	31.0	34.0	30.0	34.0
4	35.74373086797387	37.0	35.0	37.0	33.0	37.0
5	35.620309724498824	37.0	35.0	37.0	33.0	37.0
6	35.55029593915244	37.0	35.0	37.0	33.0	37.0
7	35.94583364767581	37.0	35.0	37.0	35.0	37.0
8	35.79377076089168	37.0	35.0	37.0	35.0	37.0
9	37.44491576764719	39.0	37.0	39.0	35.0	39.0
10	37.203799086006384	39.0	37.0	39.0	34.0	39.0
11	37.06804428685408	39.0	37.0	39.0	33.0	39.0
12	36.93839573317239	39.0	35.0	39.0	33.0	39.0
13	36.76603318084866	39.0	35.0	39.0	33.0	39.0
14	37.74956277819309	40.0	36.0	41.0	33.0	41.0
15	37.84263787060978	40.0	36.0	41.0	33.0	41.0
16	37.964218681773396	40.0	36.0	41.0	33.0	41.0
17	37.9731345774437	40.0	36.0	41.0	33.0	41.0
18	37.951886169159685	39.0	36.0	41.0	34.0	41.0
19	37.89988935144861	39.0	36.0	41.0	34.0	41.0
20	37.83077401405054	39.0	36.0	41.0	34.0	41.0
21	37.72045923721083	39.0	35.0	41.0	33.0	41.0
22	37.6272023976901	39.0	35.0	41.0	33.0	41.0
23	37.43398693706978	39.0	35.0	41.0	33.0	41.0
24	37.156559698779894	39.0	35.0	41.0	32.0	41.0
25	37.1108611612154	39.0	35.0	40.0	32.0	41.0
26	37.354459433817766	39.0	35.0	41.0	33.0	41.0
27	37.447777998770064	39.0	35.0	41.0	33.0	41.0
28	37.419395730886265	39.0	35.0	41.0	33.0	41.0
29	37.462827573436115	39.0	35.0	41.0	33.0	41.0
30	37.30069772598938	39.0	35.0	41.0	33.0	41.0
31	37.15299334051177	39.0	35.0	41.0	32.0	41.0
32	37.05257863705675	39.0	35.0	41.0	32.0	41.0
33	36.88314804273229	39.0	35.0	41.0	32.0	41.0
34	36.77271667341074	39.0	35.0	41.0	31.0	41.0
35	36.69875588963493	39.0	35.0	41.0	31.0	41.0
36	36.6248019642404	39.0	35.0	41.0	31.0	41.0
37	36.45984189145011	39.0	35.0	40.0	31.0	41.0
38	36.35461374739667	39.0	35.0	40.0	30.0	41.0
39	36.41968949821796	39.0	35.0	40.0	31.0	41.0
40	36.307468091381075	39.0	35.0	40.0	30.0	41.0
41	36.304867621810565	39.0	35.0	40.0	30.0	41.0
42	36.18419783229429	39.0	35.0	40.0	30.0	41.0
43	36.09247498405426	39.0	35.0	40.0	30.0	41.0
44	36.00013030924441	38.0	35.0	40.0	30.0	41.0
45	36.00202322247903	38.0	35.0	40.0	30.0	41.0
46	35.9545757976869	38.0	35.0	40.0	30.0	41.0
47	35.795747117765266	38.0	35.0	40.0	29.0	41.0
48	35.79205159331628	38.0	35.0	40.0	29.0	41.0
49	35.804869907937665	38.0	35.0	40.0	29.0	41.0
50	35.65690261784414	38.0	35.0	40.0	28.0	41.0
51	34.68490538863018	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	8.0
10	16.0
11	9.0
12	12.0
13	14.0
14	14.0
15	18.0
16	48.0
17	119.0
18	245.0
19	459.0
20	761.0
21	1246.0
22	1974.0
23	2973.0
24	4309.0
25	5864.0
26	7859.0
27	9252.0
28	10398.0
29	11937.0
30	14509.0
31	18424.0
32	23801.0
33	33421.0
34	59881.0
35	83145.0
36	70698.0
37	108751.0
38	192572.0
39	211988.0
40	112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.59090212861294	22.6666072273622	26.124374458473643	13.618116185551218
2	31.452079346899207	24.531629711422177	25.68075149569865	18.335539445979958
3	28.71729980956561	23.988331607307376	29.79932376360531	17.495044819521695
4	27.379915459020026	25.149684171541832	28.898361075485628	18.572039293952507
5	22.907679329524647	28.887730584494115	27.852457929546137	20.352132156435104
6	24.935131143680803	31.26530276324182	28.134108787643942	15.665457305433439
7	75.04955180478304	5.6415901385621625	12.50694411105091	6.801913945603892
8	73.85802236289524	4.387535120627496	12.79156693437215	8.962875582105113
9	67.98393309877669	7.097853098045133	15.817484757247593	9.10072904593058
10	37.71355284725699	26.173983416434055	21.604243966339066	14.508219769969891
11	25.50517693480651	22.16868874608215	30.261121436785153	22.06501288232618
12	24.323477839427003	19.491062386122294	36.34553439363908	19.83992538081162
13	21.226804382962865	25.34297621741983	36.5287674802993	16.901451919318003
14	16.681869411848083	29.892026217305524	33.12460992956442	20.30149444128197
15	14.005500421790448	25.017317412744244	42.06028059923963	18.916901566225672
16	15.23703708783986	25.97691926084939	35.16314946013109	23.622894191179665
17	15.227663966750567	26.067907119228384	30.68348341757712	28.02094549644393
18	17.494816206812203	24.243920616522754	35.72416504923175	22.537098127433296
19	18.022911565745588	27.177478904762232	31.67131893530489	23.12829059418729
20	21.017166528355975	27.707631778081073	30.704630093205402	20.57057160035755
21	19.947487660629005	27.994312115787768	32.29371703690495	19.76448318667828
22	16.712274902210915	26.311722573904774	29.44726019098306	27.528742332901256
23	16.318946735524815	27.206055493449107	30.357138774773045	26.117858996253034
24	20.50381668918502	23.32295431632226	31.624796248922664	24.548432745570057
25	16.199382288458946	26.775120536051084	29.83453012086754	27.190967054622433
26	15.576984186858883	26.621721407979955	31.022287453048662	26.779006952112493
27	18.841802291156576	26.698078052951274	31.369207239707286	23.090912416184867
28	13.77631618052174	26.725054352671684	33.816620601205706	25.682008865600874
29	15.34299907869078	23.630438410592998	33.30155616671353	27.72500634400269
30	19.108136097718216	26.901429058047054	31.363834841034155	22.62660000320058
31	21.0138516440683	29.61814819133055	25.07938576337213	24.288614401229022
32	19.506036518594215	26.808612297992095	27.39214623897801	26.293204944435683
33	21.166907853075184	27.207198556996577	27.84662830545401	23.779265284474224
34	20.401626808040767	24.860031868611703	29.79680902380087	24.94153229954666
35	17.384053349061887	22.93339825934283	30.032051501871194	29.65049688972409
36	23.50081500430935	25.116421022310316	28.7815399809337	22.601223992446638
37	21.27252692486186	25.854954380333822	32.53901847419306	20.333500220611263
38	18.49419666636947	28.860297059354718	27.7996483936528	24.845857880623015
39	24.495737516031465	24.658395458837138	29.157722194407675	21.68814483072372
40	24.366114109747816	22.089474442242143	29.077250520665448	24.467160927344594
41	21.43667085027925	22.14639900690639	25.839637328797654	30.577292814016705
42	23.187386979591743	20.45375050580562	29.168695604463434	27.1901669101392
43	24.073032616175265	21.742326042874026	30.39417403371123	23.790467307239478
44	19.399617302324305	24.645250228041178	29.63003605222429	26.325096417410233
45	20.446663511811273	22.11725088644578	28.828862811799162	28.60722278994378
46	24.23283290011225	22.35958035851045	29.515043859348317	23.892542882028984
47	18.985485379074163	21.912871124157277	33.21811252774787	25.88353096902069
48	19.458713687728753	19.974349653994665	31.76527875890732	28.80165789936926
49	22.52875376353673	17.751548279575054	35.26579656669433	24.453901390193884
50	20.764549484364032	18.26364074884379	33.29995587774707	27.671853889045106
51	18.768531917763436	18.55134984374321	28.486629585685186	34.19348865280816
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	671.0
1	930.0
2	1189.0
3	4352.0
4	7515.0
5	4990.5
6	2466.0
7	2459.5
8	2453.0
9	2580.0
10	2707.0
11	2753.0
12	2799.0
13	3007.5
14	3216.0
15	3034.5
16	2853.0
17	2921.5
18	2990.0
19	2773.5
20	2557.0
21	2684.0
22	2811.0
23	2969.5
24	3128.0
25	3795.0
26	5000.5
27	5539.0
28	6458.0
29	7377.0
30	8822.0
31	10267.0
32	10981.0
33	11695.0
34	13772.0
35	15849.0
36	17724.5
37	19600.0
38	21350.5
39	23101.0
40	28720.5
41	34340.0
42	42116.0
43	49892.0
44	60978.0
45	72064.0
46	88841.5
47	105619.0
48	122154.0
49	138689.0
50	132196.0
51	125703.0
52	99482.5
53	73262.0
54	58643.5
55	44025.0
56	35758.5
57	27492.0
58	24070.0
59	20648.0
60	18735.0
61	16822.0
62	14491.5
63	12161.0
64	9929.5
65	7698.0
66	6112.0
67	4526.0
68	3552.0
69	2578.0
70	2154.5
71	1731.0
72	1479.0
73	1227.0
74	931.0
75	482.5
76	330.0
77	225.0
78	120.0
79	67.0
80	14.0
81	11.5
82	9.0
83	7.0
84	5.0
85	4.5
86	4.0
87	2.0
88	0.0
89	1.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	874842.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.695927169915024
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.79480148829023	16.248636257138322
2	12.073681240487339	5.963415051756892
3	5.945022778408842	4.404535486772119
4	3.6442137076749024	3.599889452653815
5	2.3294906428522992	2.8764465629439453
6	1.6234109797811709	2.4054983594109713
7	1.1719400139280822	2.025950166203398
8	0.8946048910080775	1.7674477787348197
9	0.6667977486796585	1.4820469774610534
>10	4.398980210255249	22.306910456676047
>50	1.1669530728213768	20.47751773425487
>100	0.27515426731676806	10.267081770263802
>500	0.008875944106992517	1.3918025313668618
>1k	0.004671549529996062	2.424117605195665
>5k	0.0014014648589988185	2.358703809167398
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC	7719	0.8823307522958431	No Hit
CCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC	7295	0.8338648578829091	No Hit
CTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGCT	5431	0.6207978126335956	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4394	0.5022621227604528	No Hit
TCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC	3778	0.4318494082360015	No Hit
ACTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC	2606	0.2978823604719481	No Hit
CGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTG	2283	0.2609614078885102	No Hit
ACCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTG	1625	0.18574782646466448	No Hit
AGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTG	1517	0.173402740151936	No Hit
GCCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTG	1440	0.16460115083637958	No Hit
GGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTG	1190	0.13602456214950814	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCC	1146	0.13099508254061876	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTC	1033	0.11807846445415288	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1430635474748584E-4	0.0	0.0	0.6530322046723865	0.0
2	1.1430635474748584E-4	0.0	0.0	3.2469863129570826	0.0
3	1.1430635474748584E-4	0.0	0.0	4.643352742552369	0.0
4	1.1430635474748584E-4	0.0	0.0	6.094128997007459	0.0
5	1.1430635474748584E-4	0.0	0.0	9.735129314779126	0.0
6	1.1430635474748584E-4	0.0	0.0	11.591464515878295	0.0
7	1.1430635474748584E-4	0.0	0.0	13.73173670217022	0.0
8	1.1430635474748584E-4	0.0	0.0	17.03210408279438	0.0
9	1.1430635474748584E-4	0.0	0.0	18.265926875938742	0.0
10	1.1430635474748584E-4	0.0	0.0	20.709796740439987	0.0
11	1.1430635474748584E-4	0.0	0.0	24.220144894735277	0.0
12	1.1430635474748584E-4	0.0	0.0	27.154160408393743	0.0
13	1.1430635474748584E-4	0.0	0.0	28.156512833174446	0.0
14	1.1430635474748584E-4	0.0	0.0	28.506290278701755	0.0
15	1.1430635474748584E-4	0.0	0.0	29.16218014224283	0.0
16	1.1430635474748584E-4	0.0	0.0	30.66233674194883	0.0
17	1.1430635474748584E-4	0.0	0.0	32.63000633257205	0.0
18	1.1430635474748584E-4	0.0	0.0	34.96128443764702	0.0
19	1.1430635474748584E-4	0.0	0.0	36.19636460069361	0.0
20	1.1430635474748584E-4	0.0	0.0	37.21654881681492	0.0
21	1.1430635474748584E-4	0.0	0.0	38.423052391174636	0.0
22	1.1430635474748584E-4	0.0	0.0	39.66807720708425	0.0
23	1.1430635474748584E-4	0.0	0.0	40.8342306382181	0.0
24	1.1430635474748584E-4	0.0	0.0	41.7506246842287	0.0
25	1.1430635474748584E-4	0.0	0.0	42.49464474728008	0.0
26	1.1430635474748584E-4	0.0	0.0	43.173281575415906	0.0
27	1.1430635474748584E-4	0.0	0.0	43.83305785501839	0.0
28	2.2861270949497167E-4	0.0	0.0	44.470773008154616	0.0
29	3.429190642424575E-4	0.0	0.0	45.187016627002365	0.0
30	3.429190642424575E-4	0.0	0.0	45.97847382727395	0.0
31	3.429190642424575E-4	0.0	0.0	46.68648738857988	0.0
32	3.429190642424575E-4	0.0	0.0	47.31494372698156	0.0
33	4.5722541898994334E-4	0.0	0.0	47.929226077394546	0.0
34	4.5722541898994334E-4	0.0	0.0	48.55722519037723	0.0
35	5.715317737374292E-4	0.0	0.0	49.236319243932044	0.0
36	5.715317737374292E-4	0.0	0.0	49.85117312611877	0.0
37	5.715317737374292E-4	0.0	0.0	50.446594927998426	0.0
38	5.715317737374292E-4	0.0	0.0	51.03504404223848	0.0
39	5.715317737374292E-4	0.0	0.0	51.66132855989996	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGGAT	25	3.8900384E-5	45.000004	20
AAGTATC	35	1.2114833E-7	45.000004	34
AAGTACG	25	3.8900384E-5	45.000004	1
CAACGGA	30	2.1649048E-6	45.000004	36
TTAGCCG	30	2.1649048E-6	45.000004	18
CCTACTA	30	2.1649048E-6	45.000004	8
GTTAGCG	25	3.8900384E-5	45.000004	1
TTCGTAG	50	2.1827873E-11	45.000004	1
CTAAGTA	30	2.1649048E-6	45.000004	13
TACGGTG	25	3.8900384E-5	45.000004	24
CACGAAA	25	3.8900384E-5	45.000004	12
CCGTCCA	25	3.8900384E-5	45.000004	20
ATCGTAG	50	2.1827873E-11	45.000004	1
TAACCTA	50	2.1827873E-11	45.000004	12
ACCTAAC	35	1.2114833E-7	45.000004	38
TGACCGG	35	1.2114833E-7	45.000004	2
ATATACG	25	3.8900384E-5	45.000004	1
CTAAACG	25	3.8900384E-5	45.000004	45
TACGAAC	25	3.8900384E-5	45.000004	2
CGCTACC	30	2.1649048E-6	45.000004	17
>>END_MODULE