##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934756.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1015128
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03205310069272	33.0	31.0	34.0	30.0	34.0
2	32.197945480766954	34.0	31.0	34.0	30.0	34.0
3	32.217156851155714	34.0	31.0	34.0	30.0	34.0
4	35.818156922082736	37.0	35.0	37.0	35.0	37.0
5	35.69233436571545	37.0	35.0	37.0	33.0	37.0
6	35.61341131364715	37.0	35.0	37.0	33.0	37.0
7	35.97043131506569	37.0	35.0	37.0	35.0	37.0
8	35.79071900292377	37.0	35.0	37.0	35.0	37.0
9	37.47519918670355	39.0	37.0	39.0	35.0	39.0
10	37.234639375527024	39.0	37.0	39.0	34.0	39.0
11	37.100572538635525	39.0	37.0	39.0	34.0	39.0
12	36.9266496441828	39.0	35.0	39.0	33.0	39.0
13	36.764912405135114	39.0	35.0	39.0	33.0	39.0
14	37.835185316531515	40.0	36.0	41.0	33.0	41.0
15	37.9394125666911	40.0	36.0	41.0	33.0	41.0
16	38.04562774349639	40.0	36.0	41.0	33.0	41.0
17	38.04786194450355	40.0	36.0	41.0	34.0	41.0
18	38.03782774192023	40.0	36.0	41.0	34.0	41.0
19	37.98902896974569	40.0	36.0	41.0	34.0	41.0
20	37.91759167316831	40.0	36.0	41.0	34.0	41.0
21	37.824210345887415	39.0	36.0	41.0	33.0	41.0
22	37.75709861219472	39.0	35.0	41.0	33.0	41.0
23	37.57636376890402	39.0	35.0	41.0	33.0	41.0
24	37.301592508530945	39.0	35.0	41.0	32.0	41.0
25	37.24253296136054	39.0	35.0	41.0	32.0	41.0
26	37.440824211330984	39.0	35.0	41.0	33.0	41.0
27	37.5339523685683	39.0	35.0	41.0	33.0	41.0
28	37.52987800553231	39.0	35.0	41.0	33.0	41.0
29	37.57421921176443	39.0	36.0	41.0	33.0	41.0
30	37.445375361530765	39.0	35.0	41.0	33.0	41.0
31	37.33714861574107	39.0	35.0	41.0	33.0	41.0
32	37.208783522866085	39.0	35.0	41.0	32.0	41.0
33	37.101305451135225	39.0	35.0	41.0	32.0	41.0
34	37.03912610035385	39.0	35.0	41.0	32.0	41.0
35	36.93146381540062	39.0	35.0	41.0	32.0	41.0
36	36.878876358449375	39.0	35.0	41.0	31.0	41.0
37	36.80430349670189	39.0	35.0	41.0	31.0	41.0
38	36.67869273628548	39.0	35.0	41.0	31.0	41.0
39	36.742056174196755	39.0	35.0	41.0	31.0	41.0
40	36.62011884215587	39.0	35.0	41.0	31.0	41.0
41	36.54596563192031	39.0	35.0	41.0	31.0	41.0
42	36.47405844386127	39.0	35.0	41.0	31.0	41.0
43	36.41558404457369	39.0	35.0	41.0	31.0	41.0
44	36.32883143800585	39.0	35.0	40.0	31.0	41.0
45	36.285176844693474	39.0	35.0	40.0	31.0	41.0
46	36.19632795076089	39.0	35.0	40.0	30.0	41.0
47	36.06379885098234	38.0	35.0	40.0	30.0	41.0
48	36.038747822934646	38.0	35.0	40.0	30.0	41.0
49	36.05378435034794	38.0	35.0	40.0	30.0	41.0
50	35.92283633196996	38.0	35.0	40.0	30.0	41.0
51	34.95566864474234	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	9.0
10	15.0
11	12.0
12	13.0
13	14.0
14	21.0
15	26.0
16	61.0
17	139.0
18	239.0
19	478.0
20	833.0
21	1336.0
22	2066.0
23	2996.0
24	4293.0
25	6132.0
26	8254.0
27	10121.0
28	11020.0
29	12598.0
30	15319.0
31	19835.0
32	26279.0
33	37204.0
34	68071.0
35	95632.0
36	79401.0
37	121437.0
38	217126.0
39	273995.0
40	147.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.14715385645948	21.344401888234785	25.019504929427622	13.488939325878116
2	31.02091558897006	23.43073976877793	27.14711839295143	18.40122624930058
3	29.650842061296707	23.967223837782033	28.78494140640392	17.59699269451734
4	27.613759053045527	25.876638217052434	27.948593674886318	18.561009055015724
5	23.327304536964796	28.84128898030593	27.824175867476807	20.00723061525246
6	26.174039135951325	32.387639785327565	25.9424427264345	15.495878352286606
7	76.90498144076412	5.830102213710981	11.244591815022343	6.020324530502557
8	75.67764853299288	4.758710231616112	11.558148332033005	8.005492903358
9	70.00644253729578	6.688713147504551	14.865908535672348	8.438935779527311
10	40.037512510737564	23.328191124666052	21.109357637657517	15.524938726938869
11	28.920096775973082	22.5877918843732	27.546181368260946	20.945929971392772
12	25.758919072274626	20.67374754710736	32.66100432654798	20.906329054070028
13	21.02079737727656	26.862917779826777	34.00792806424411	18.108356778652546
14	17.224724369734652	29.635376031397026	31.286694879857514	21.853204719010805
15	15.837017597780772	24.89065418351183	39.5026045976468	19.769723621060596
16	17.259596819317366	24.51946946591957	34.63533662749919	23.58559708726387
17	17.944830602643215	24.63295269168026	29.933565028252595	27.48865167742393
18	19.20871062565509	24.397218872890907	34.164164519154234	22.22990598229977
19	19.92162564720902	26.58127842006131	30.243772213947405	23.253323718782262
20	21.857736167261667	26.87739871228062	29.607793302913528	21.65707181754419
21	20.660153202354778	27.356353090447705	30.65485337809616	21.328640329101354
22	19.56905927134312	24.958822926763915	27.42826520399398	28.043852597898983
23	18.189824337423456	26.79258182219385	29.463673546587227	25.553920293795464
24	20.59109787140144	23.59328084734142	31.257831524694424	24.55778975656272
25	18.294638705660766	25.834870085348843	29.504653600334148	26.36583760865625
26	19.412428777454664	26.52099045637594	29.477464910828978	24.58911585534041
27	20.457518657745624	25.92697669653482	29.038998037685886	24.576506608033664
28	16.91067530400107	24.18975735079714	32.961360537784394	25.93820680741739
29	20.27931452979329	21.249734023689623	30.159644892072723	28.311306554444364
30	20.805356565871495	23.339224216059453	28.23545405111474	27.619965166954312
31	21.129749154786392	24.68378371988557	28.331599561828657	25.854867563499383
32	21.890342892718948	25.821374250340845	25.38192227975191	26.906360577188295
33	19.730910781694526	23.358827655231657	28.06325901758202	28.8470025454918
34	18.396793310794305	22.440618325964802	32.538852243263904	26.623736119976986
35	18.482890827560663	23.275291391824478	27.96967476022728	30.272143020387578
36	20.892439180083695	22.363977744678504	31.20030183385741	25.5432812413804
37	18.562880740162818	23.81689796754695	33.49065339543388	24.129567896856358
38	16.974115579513125	26.575761874364613	29.03436807969044	27.415754466431817
39	19.515864009267798	22.301621076356874	32.36695273896494	25.81556217541039
40	21.515710334066245	21.770653553049467	31.97764222836923	24.735993884515057
41	17.958326437651213	24.199903854489285	29.691428076065286	28.15034163179422
42	21.34558400516979	21.817839720705173	31.460072030325243	25.376504243799797
43	21.909256763679064	20.495050870432102	31.22473225051422	26.370960115374615
44	19.740564736663753	21.699726536949036	30.338538588237146	28.221170138150065
45	20.141203867886613	21.30943092890749	28.46508026573989	30.084284937466016
46	22.462388979517854	22.09741037583438	30.073744394795533	25.366456249852238
47	17.4856766831375	22.26172462980038	34.00014579442198	26.25245289264014
48	18.80708639698639	20.723691987611414	32.440145479190804	28.029076136211394
49	20.86574303930145	18.016250167466566	34.96623085955663	26.15177593367536
50	19.311653308745303	19.093651243981054	33.18921357700704	28.40548187026661
51	18.06520951052478	19.93561403093994	29.09761133571333	32.90156512282195
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	455.0
1	671.0
2	887.0
3	4043.0
4	7199.0
5	4813.5
6	2428.0
7	2442.5
8	2457.0
9	2513.5
10	2570.0
11	2728.5
12	2887.0
13	2905.0
14	2923.0
15	2693.5
16	2464.0
17	2544.0
18	2624.0
19	2569.5
20	2515.0
21	2616.5
22	2718.0
23	3296.0
24	3874.0
25	4364.5
26	5581.0
27	6307.0
28	7409.5
29	8512.0
30	9864.0
31	11216.0
32	13510.0
33	15804.0
34	17266.0
35	18728.0
36	19990.5
37	21253.0
38	24597.0
39	27941.0
40	32082.5
41	36224.0
42	46972.5
43	57721.0
44	66476.5
45	75232.0
46	91231.0
47	107230.0
48	123954.5
49	140679.0
50	138253.5
51	135828.0
52	111287.0
53	86746.0
54	73235.5
55	59725.0
56	51198.5
57	42672.0
58	37838.0
59	33004.0
60	30304.5
61	27605.0
62	23827.5
63	20050.0
64	17067.5
65	14085.0
66	12034.0
67	9983.0
68	8471.0
69	6959.0
70	5739.0
71	4519.0
72	3694.5
73	2870.0
74	2064.5
75	1348.0
76	1437.0
77	934.0
78	431.0
79	299.5
80	168.0
81	108.0
82	48.0
83	35.5
84	23.0
85	14.0
86	5.0
87	5.5
88	6.0
89	3.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1015128.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.936675162690392
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.35123726945973	15.882280131616538
2	12.283669702816374	5.880604229641943
3	5.9561634598230455	4.277122498610111
4	3.450530719542678	3.303769318903097
5	2.2210524781387297	2.658230584424764
6	1.4995953439647218	2.1537195974379904
7	1.079724912892715	1.8091517153483974
8	0.8183994654024569	1.5671809725326467
9	0.6104229654871838	1.3150346613041597
>10	3.963796168731981	19.66039853806161
>50	1.33070500637432	23.159923048297955
>100	0.4238972825175929	13.819197032299193
>500	0.006649369137530864	1.1007170788694804
>1k	0.003324684568765432	1.9839766309058218
>5k	8.31171142191358E-4	1.4286939617463605
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	8035	0.7915257977319117	No Hit
CCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	6327	0.623271153982552	No Hit
CTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGCT	4495	0.44280130190478445	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4322	0.42575911609176387	No Hit
TCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	3101	0.3054787179547801	No Hit
ACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	2179	0.21465273344839272	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTG	1767	0.1740667186798118	No Hit
ACCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTG	1469	0.1447108147937994	No Hit
GCCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTG	1394	0.13732258395000432	No Hit
AGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTG	1217	0.11988635915864798	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4665421503495126	0.0
2	0.0	0.0	0.0	2.517219503353272	0.0
3	0.0	0.0	0.0	3.571372280145952	0.0
4	0.0	0.0	0.0	4.694974426870306	0.0
5	0.0	0.0	0.0	7.589486251980046	0.0
6	0.0	0.0	0.0	9.08663735016668	0.0
7	0.0	0.0	0.0	10.78780212938664	0.0
8	0.0	0.0	0.0	13.598285142366283	0.0
9	0.0	0.0	0.0	14.621407349615025	0.0
10	0.0	0.0	0.0	16.735229448897083	0.0
11	0.0	0.0	0.0	19.5409839941367	0.0
12	0.0	0.0	0.0	21.710759628342437	0.0
13	0.0	0.0	0.0	22.536763836678723	0.0
14	0.0	0.0	0.0	22.862043013294876	0.0
15	0.0	0.0	0.0	23.445417720720933	0.0
16	0.0	0.0	0.0	24.744564232293857	0.0
17	0.0	0.0	0.0	26.295403141278737	0.0
18	0.0	0.0	0.0	28.179500516191062	0.0
19	0.0	0.0	0.0	29.135833116611895	0.0
20	0.0	0.0	0.0	30.00656074898929	0.0
21	0.0	0.0	0.0	31.10001891387096	0.0
22	0.0	0.0	0.0	32.15692996351199	0.0
23	0.0	0.0	0.0	33.27255282092504	0.0
24	0.0	0.0	0.0	34.113136471459754	0.0
25	0.0	0.0	0.0	34.85993884515056	0.0
26	0.0	0.0	0.0	35.53689780993136	0.0
27	0.0	0.0	0.0	36.16095704187058	0.0
28	0.0	0.0	0.0	36.76945173416554	0.0
29	0.0	0.0	0.0	37.420502636120766	0.0
30	0.0	0.0	0.0	38.2002072645026	0.0
31	9.850974458393424E-5	0.0	0.0	38.89243523969391	0.0
32	9.850974458393424E-5	0.0	0.0	39.49915675658636	0.0
33	9.850974458393424E-5	0.0	0.0	40.10617380271256	0.0
34	9.850974458393424E-5	0.0	0.0	40.70343838412496	0.0
35	9.850974458393424E-5	0.0	0.0	41.37616142988865	0.0
36	9.850974458393424E-5	0.0	0.0	42.0190360230434	0.0
37	9.850974458393424E-5	0.0	0.0	42.592658265755645	0.0
38	1.9701948916786848E-4	0.0	0.0	43.18450481121592	0.0
39	1.9701948916786848E-4	0.0	0.0	43.73635640037512	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGAA	35	1.211738E-7	45.000004	9
GCGCAAT	25	3.8904822E-5	45.000004	9
TAACCTA	25	3.8904822E-5	45.000004	14
TACGCAC	25	3.8904822E-5	45.000004	28
TGCGATC	25	3.8904822E-5	45.000004	38
CTAAACG	25	3.8904822E-5	45.000004	1
CCGAACC	25	3.8904822E-5	45.000004	18
GTGACCG	25	3.8904822E-5	45.000004	1
TGTACCG	25	3.8904822E-5	45.000004	21
CGTCACA	25	3.8904822E-5	45.000004	43
CCGTTAG	25	3.8904822E-5	45.000004	1
ATCGAGC	25	3.8904822E-5	45.000004	20
TGCGTAG	25	3.8904822E-5	45.000004	1
TGTCCGA	25	3.8904822E-5	45.000004	39
TTAGGTA	105	0.0	45.0	1
TCGTTGC	20	7.0328964E-4	45.0	44
TCCGCTT	20	7.0328964E-4	45.0	15
CGTATCT	20	7.0328964E-4	45.0	6
AGTCACG	20	7.0328964E-4	45.0	11
CTATCGA	20	7.0328964E-4	45.0	28
>>END_MODULE