FastQCFastQC Report
Sat 14 Jan 2017
SRR2934754.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934754.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2538588
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGC73770.2905946140137746No Hit
CCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGC56080.22091020677636547No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47110.18557560344569501No Hit
CTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGCT44780.17639727281465128No Hit
TCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGC32190.1268027738254494No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGA207.0348993E-445.040
TACCGTA253.8921433E-545.039
GTACGAC207.0348993E-445.022
TATAGCG1800.041.251
CGTTTTT30650.040.0081561
CGTAGAT403.4604273E-739.37515
CGTAAGG5500.038.0454562
GCGCCTA301.14031085E-437.49999634
ATACCGG1450.037.2413832
CACGCGA250.002107587436.012
CGTCTAG504.882895E-836.038
CGTACCC250.002107587436.028
CGAGCGT250.002107587436.015
CGTAATC250.002107587436.029
CCGTTCG250.002107587436.031
ATCGCGT250.002107587436.02
TCTATCG250.002107587436.042
TTGTGCG3400.035.7352941
TTACGCG1200.035.6251
AGGGCGA22000.035.3863646
Produced by FastQC (version 0.11.5)