##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934754.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2538588
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33081933736392	33.0	31.0	34.0	30.0	34.0
2	32.50369536135836	34.0	31.0	34.0	31.0	34.0
3	32.51642803007026	34.0	31.0	34.0	31.0	34.0
4	36.02402871202416	37.0	35.0	37.0	35.0	37.0
5	35.92507685374704	37.0	35.0	37.0	35.0	37.0
6	35.86727306675995	37.0	35.0	37.0	35.0	37.0
7	36.15656735161436	37.0	35.0	37.0	35.0	37.0
8	36.014517125268064	37.0	36.0	37.0	35.0	37.0
9	37.70274420268275	39.0	38.0	39.0	35.0	39.0
10	37.42494095142654	39.0	37.0	39.0	35.0	39.0
11	37.28753031212627	39.0	37.0	39.0	34.0	39.0
12	37.25223903997025	39.0	37.0	39.0	34.0	39.0
13	37.23914475290989	39.0	37.0	39.0	34.0	39.0
14	38.4430002032626	40.0	38.0	41.0	34.0	41.0
15	38.47720504469414	40.0	38.0	41.0	34.0	41.0
16	38.5585494771109	40.0	38.0	41.0	35.0	41.0
17	38.553876012964686	40.0	38.0	41.0	34.0	41.0
18	38.529575102379745	40.0	38.0	41.0	34.0	41.0
19	38.51613455984193	40.0	38.0	41.0	34.0	41.0
20	38.48161143123658	40.0	38.0	41.0	34.0	41.0
21	38.41533206648735	40.0	37.0	41.0	34.0	41.0
22	38.3739992468254	40.0	37.0	41.0	34.0	41.0
23	38.20414537530312	40.0	37.0	41.0	34.0	41.0
24	37.98427432887889	40.0	37.0	41.0	34.0	41.0
25	37.91321671732475	40.0	36.0	41.0	33.0	41.0
26	38.06291607775661	40.0	37.0	41.0	34.0	41.0
27	38.13645656561837	40.0	37.0	41.0	34.0	41.0
28	38.11953889327453	40.0	37.0	41.0	34.0	41.0
29	38.11537082819268	40.0	37.0	41.0	34.0	41.0
30	38.007293818453405	40.0	37.0	41.0	34.0	41.0
31	37.96299557076611	40.0	37.0	41.0	34.0	41.0
32	37.89610799389267	40.0	37.0	41.0	34.0	41.0
33	37.80499553294981	40.0	37.0	41.0	33.0	41.0
34	37.744151079261385	40.0	37.0	41.0	33.0	41.0
35	37.68729269972126	40.0	36.0	41.0	33.0	41.0
36	37.63416631607807	40.0	36.0	41.0	33.0	41.0
37	37.571733183958955	40.0	36.0	41.0	33.0	41.0
38	37.50188411825787	40.0	36.0	41.0	33.0	41.0
39	37.516311035898696	40.0	36.0	41.0	33.0	41.0
40	37.45804557494166	40.0	36.0	41.0	33.0	41.0
41	37.394644975868474	40.0	36.0	41.0	33.0	41.0
42	37.30032482624199	40.0	35.0	41.0	33.0	41.0
43	37.24354562457555	40.0	35.0	41.0	33.0	41.0
44	37.189172090941895	39.0	35.0	41.0	32.0	41.0
45	37.13097320242591	39.0	35.0	41.0	32.0	41.0
46	37.107013426361426	39.0	35.0	41.0	32.0	41.0
47	37.00177894167939	39.0	35.0	41.0	32.0	41.0
48	36.948558017291504	39.0	35.0	41.0	32.0	41.0
49	36.913968316245096	39.0	35.0	41.0	32.0	41.0
50	36.80311929308734	39.0	35.0	41.0	32.0	41.0
51	35.89761473701128	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	27.0
10	33.0
11	30.0
12	30.0
13	32.0
14	43.0
15	80.0
16	137.0
17	229.0
18	405.0
19	785.0
20	1361.0
21	2318.0
22	3485.0
23	4924.0
24	7181.0
25	10347.0
26	13846.0
27	16804.0
28	19810.0
29	23444.0
30	29163.0
31	37928.0
32	50780.0
33	72349.0
34	137867.0
35	189810.0
36	176333.0
37	280864.0
38	534508.0
39	922940.0
40	687.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.199941069602474	22.26560591951116	27.245579038426087	11.288873972460282
2	30.112566513353094	25.06881778374435	27.522386460504816	17.296229242397743
3	31.044344336300338	24.108441385526127	28.137531572669534	16.709682705504004
4	27.41945522471547	26.426895581323162	27.74869336812433	18.404955825837042
5	25.863235783041596	29.655895324487467	26.011310224423973	18.46955866804696
6	27.506393317860166	33.89199035054132	23.545569426783707	15.056046904814801
7	83.03848438580818	5.302750978102788	7.26911968385575	4.389644952233289
8	85.44356153893425	3.579155026337476	6.57743596046306	4.399847474265221
9	79.64037488556632	6.329817993309667	8.490940633139367	5.5388664879846585
10	40.79960986186021	30.285379116264632	15.686791239854598	13.228219782020556
11	26.72966231621673	26.28914183790359	27.374508979007228	19.60668686687245
12	25.196172045247202	23.23902106210224	30.4531889381026	21.111617954547963
13	23.362278558001535	24.811942702005997	31.432788621075968	20.3929901189165
14	20.254921239681273	27.900037343594153	29.70222028938922	22.142821127335353
15	19.051220599797997	27.161358991691444	33.33652408346687	20.450896325043686
16	22.10709260423511	26.59639925817029	30.42494489062424	20.87156324697036
17	22.15077830668072	25.75916217992049	28.317119595617722	23.772939917781066
18	21.995219389676464	25.735172465953514	30.88858845941129	21.381019684958723
19	22.84967076185659	27.153323028392162	28.558080318665336	21.438925891085912
20	24.71074471320277	27.011117991576423	28.525227409882977	19.752909885337832
21	23.545963346553282	27.31321506286172	28.851235411181335	20.289586179403667
22	23.062505613356716	24.74686715607259	27.935293162970908	24.255334067599783
23	20.87609332432045	26.767202870257005	28.94837602635796	23.40832777906458
24	21.83989682453395	25.473373387095506	29.88728379713447	22.799445991236073
25	20.953025855317993	27.265393202835593	27.98189387171136	23.79968707013505
26	20.83225005396701	27.81995345444003	28.876131140618327	22.471665350974636
27	21.714354593971137	27.481615764354046	28.531175598403525	22.2728540432713
28	19.32207983335618	26.96770803296951	29.65680133995749	24.05341079371682
29	20.493754795973196	25.559878168493665	30.01507137038385	23.93129566514929
30	22.180243505444757	26.14492781026303	29.71116226815852	21.96366641613369
31	22.1623201559292	26.755227709261998	27.226316361693982	23.85613577311482
32	22.52023565856295	26.91712873455638	27.31837541184312	23.244260195037555
33	23.465997633330023	25.98160867379819	27.808411605191548	22.743982087680237
34	20.70324132943195	25.221264734568983	29.891853266461517	24.183640669537553
35	20.45385072331548	25.85303326100966	29.112167866546283	24.58094814912857
36	22.667561652383135	26.025845863921205	28.98197738270251	22.32461510099315
37	22.10843193145166	27.005800074687187	28.673144283357523	22.212623710503635
38	20.665818951322546	27.91528203867662	27.318611763704865	24.100287246295974
39	22.319179008173048	26.060707763528384	29.498445592589267	22.1216676357093
40	21.764736932499485	23.774042893135867	31.583778068753183	22.877442105611465
41	20.84721900521077	24.895374909201493	28.391097728343475	25.86630835724426
42	22.503966772079597	24.453239359833105	28.33551564885677	24.70727821923053
43	23.238902886171367	24.766838888389923	28.087740113795544	23.906518111643166
44	21.00258096233024	25.706416322774707	28.35135122359359	24.939651491301465
45	21.597517990315875	24.928858089615172	28.49962262486075	24.974001295208204
46	22.16614117769406	24.96943182588116	28.83165759863357	24.032769397791213
47	20.166210507573503	25.049279363173543	30.637228254446956	24.147281874805994
48	21.400755065414316	23.359402943683655	30.049027254521015	25.19081473638101
49	22.31126122080464	23.0350494054175	31.157399310167698	23.496290063610164
50	20.600152525734778	23.61363876296587	30.685128898427	25.10107981287235
51	20.62020304200603	22.811499936184997	28.884521631710225	27.68377539009875
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	359.0
1	934.5
2	1510.0
3	5150.5
4	8791.0
5	6080.0
6	3369.0
7	3360.0
8	3351.0
9	3455.5
10	3560.0
11	3715.0
12	3870.0
13	4007.0
14	4144.0
15	4111.0
16	4078.0
17	4117.0
18	4156.0
19	4701.0
20	5246.0
21	6165.5
22	7085.0
23	8078.0
24	9071.0
25	10129.0
26	14755.5
27	18324.0
28	23237.5
29	28151.0
30	34662.5
31	41174.0
32	48501.5
33	55829.0
34	63263.0
35	70697.0
36	78914.0
37	87131.0
38	96408.0
39	105685.0
40	115808.0
41	125931.0
42	141892.5
43	157854.0
44	172245.0
45	186636.0
46	213079.5
47	239523.0
48	258852.5
49	278182.0
50	268478.0
51	258774.0
52	226655.5
53	194537.0
54	168102.5
55	141668.0
56	128989.0
57	116310.0
58	106996.5
59	97683.0
60	90283.5
61	82884.0
62	73735.0
63	64586.0
64	54601.0
65	44616.0
66	36490.0
67	28364.0
68	22806.0
69	17248.0
70	14938.5
71	12629.0
72	10242.0
73	7855.0
74	5412.5
75	2363.0
76	1756.0
77	1323.5
78	891.0
79	676.0
80	461.0
81	393.0
82	325.0
83	180.5
84	36.0
85	48.0
86	60.0
87	41.5
88	23.0
89	14.0
90	5.0
91	4.5
92	4.0
93	5.5
94	7.0
95	4.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2538588.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.335813890949943
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.3470171709313	13.695564072986684
2	12.721193267348779	5.173916375112204
3	5.6068133684450165	3.42057339545964
4	3.015861673647931	2.453200068646125
5	1.8415001015728547	1.8724201672875491
6	1.170405020681418	1.4280683206566442
7	0.7924223781612607	1.1280187803717947
8	0.6080393890669957	0.9891980683546662
9	0.45840001872634206	0.8389743721584174
>10	3.8436347764795107	17.750787637006084
>50	1.678813377102447	24.99203211868765
>100	0.9089593647353897	24.185664320683046
>500	0.004560632609099374	0.6179584768012254
>1k	0.0019828837430866837	0.9300224671414984
>5k	3.9657674861733684E-4	0.523601358646911
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGC	7377	0.2905946140137746	No Hit
CCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGC	5608	0.22091020677636547	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4711	0.18557560344569501	No Hit
CTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGCT	4478	0.17639727281465128	No Hit
TCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGC	3219	0.1268027738254494	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1817593087180748E-4	0.0	0.0	0.18565438739960954	0.0
2	1.1817593087180748E-4	0.0	0.0	0.9473376538453661	0.0
3	1.5756790782907665E-4	0.0	0.0	1.3804918324674977	0.0
4	1.5756790782907665E-4	0.0	0.0	1.8680069392906609	0.0
5	1.5756790782907665E-4	0.0	0.0	3.144661520498797	0.0
6	1.5756790782907665E-4	0.0	0.0	3.875303909102225	0.0
7	1.5756790782907665E-4	0.0	0.0	4.643053539999401	0.0
8	1.5756790782907665E-4	0.0	0.0	5.919708121207537	0.0
9	1.5756790782907665E-4	0.0	0.0	6.395838946690049	0.0
10	1.969598847863458E-4	0.0	0.0	7.417824396869441	0.0
11	1.969598847863458E-4	0.0	0.0	8.939575858705705	0.0
12	1.969598847863458E-4	0.0	0.0	10.133270936441834	0.0
13	2.3635186174361496E-4	0.0	0.0	10.567606874372682	0.0
14	2.3635186174361496E-4	0.0	0.0	10.722299167883879	0.0011423673317608057
15	2.3635186174361496E-4	0.0	0.0	11.043304388108664	0.0011423673317608057
16	2.3635186174361496E-4	0.0	0.0	11.746648136680706	0.0011423673317608057
17	2.3635186174361496E-4	0.0	0.0	12.701115738355337	0.0011423673317608057
18	2.3635186174361496E-4	0.0	0.0	13.740315482465055	0.0011423673317608057
19	2.3635186174361496E-4	0.0	0.0	14.38488640141685	0.0011423673317608057
20	2.7574383870088414E-4	0.0	0.0	14.974544904490212	0.0011423673317608057
21	2.7574383870088414E-4	0.0	0.0	15.698096737241332	0.0011423673317608057
22	2.7574383870088414E-4	0.0	0.0	16.474551995046063	0.0011423673317608057
23	2.7574383870088414E-4	0.0	0.0	17.28386016163316	0.0011423673317608057
24	2.7574383870088414E-4	0.0	0.0	17.938357858778186	0.0011423673317608057
25	2.7574383870088414E-4	0.0	0.0	18.483267076028092	0.0011423673317608057
26	2.7574383870088414E-4	0.0	0.0	18.986381405726334	0.0011423673317608057
27	2.7574383870088414E-4	0.0	0.0	19.47755996640652	0.0011423673317608057
28	2.7574383870088414E-4	0.0	0.0	19.990561682321037	0.0011423673317608057
29	2.7574383870088414E-4	0.0	0.0	20.54736727661204	0.0011423673317608057
30	2.7574383870088414E-4	0.0	0.0	21.16314266040807	0.0011423673317608057
31	3.151358156581533E-4	0.0	0.0	21.723178396809566	0.0011423673317608057
32	3.5452779261542245E-4	0.0	0.0	22.254418598055295	0.0011423673317608057
33	3.939197695726916E-4	0.0	0.0	22.7687202492094	0.0011423673317608057
34	4.3331174652996076E-4	0.0	0.0	23.29239719087934	0.0011423673317608057
35	4.3331174652996076E-4	0.0	0.0	23.858065979985724	0.0011423673317608057
36	4.3331174652996076E-4	0.0	0.0	24.40785980237833	0.0011423673317608057
37	4.3331174652996076E-4	0.0	0.0	24.93007924090085	0.0011423673317608057
38	4.3331174652996076E-4	0.0	0.0	25.46809486218323	0.0011423673317608057
39	4.3331174652996076E-4	0.0	0.0	25.997680600396755	0.0011423673317608057
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCGA	20	7.0348993E-4	45.0	40
TACCGTA	25	3.8921433E-5	45.0	39
GTACGAC	20	7.0348993E-4	45.0	22
TATAGCG	180	0.0	41.25	1
CGTTTTT	3065	0.0	40.008156	1
CGTAGAT	40	3.4604273E-7	39.375	15
CGTAAGG	550	0.0	38.045456	2
GCGCCTA	30	1.14031085E-4	37.499996	34
ATACCGG	145	0.0	37.241383	2
CACGCGA	25	0.0021075874	36.0	12
CGTCTAG	50	4.882895E-8	36.0	38
CGTACCC	25	0.0021075874	36.0	28
CGAGCGT	25	0.0021075874	36.0	15
CGTAATC	25	0.0021075874	36.0	29
CCGTTCG	25	0.0021075874	36.0	31
ATCGCGT	25	0.0021075874	36.0	2
TCTATCG	25	0.0021075874	36.0	42
TTGTGCG	340	0.0	35.735294	1
TTACGCG	120	0.0	35.625	1
AGGGCGA	2200	0.0	35.386364	6
>>END_MODULE