##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934752.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1895161
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.943789472240088	33.0	31.0	34.0	30.0	34.0
2	32.12354939765012	33.0	31.0	34.0	30.0	34.0
3	32.119495388518445	33.0	31.0	34.0	30.0	34.0
4	35.74404602036449	37.0	35.0	37.0	33.0	37.0
5	35.6274670067609	37.0	35.0	37.0	33.0	37.0
6	35.555876783027934	37.0	35.0	37.0	33.0	37.0
7	35.961920913315545	37.0	35.0	37.0	35.0	37.0
8	35.832360416872234	37.0	35.0	37.0	35.0	37.0
9	37.53256003051983	39.0	37.0	39.0	35.0	39.0
10	37.13364088855775	39.0	37.0	39.0	34.0	39.0
11	37.019371968925064	39.0	37.0	39.0	33.0	39.0
12	36.97610176655176	39.0	37.0	39.0	33.0	39.0
13	36.922364379596246	39.0	37.0	39.0	33.0	39.0
14	37.98267376755854	40.0	37.0	41.0	33.0	41.0
15	38.04104717224553	40.0	37.0	41.0	33.0	41.0
16	38.14554225208307	40.0	37.0	41.0	33.0	41.0
17	38.138462642487895	40.0	37.0	41.0	34.0	41.0
18	38.12461315951521	40.0	37.0	41.0	34.0	41.0
19	38.049437488424466	40.0	37.0	41.0	34.0	41.0
20	37.994293888487576	40.0	36.0	41.0	34.0	41.0
21	37.933210423810955	40.0	36.0	41.0	34.0	41.0
22	37.87788530895264	39.0	36.0	41.0	33.0	41.0
23	37.673187132913775	39.0	36.0	41.0	33.0	41.0
24	37.403745117169464	39.0	35.0	41.0	32.0	41.0
25	37.3425571758811	39.0	35.0	41.0	32.0	41.0
26	37.53174426869274	39.0	36.0	41.0	33.0	41.0
27	37.673053107361326	39.0	36.0	41.0	33.0	41.0
28	37.64767215028169	39.0	36.0	41.0	33.0	41.0
29	37.60574906300837	39.0	36.0	41.0	33.0	41.0
30	37.51942869233801	39.0	36.0	41.0	33.0	41.0
31	37.43406655160168	39.0	36.0	41.0	33.0	41.0
32	37.28768690364566	39.0	35.0	41.0	32.0	41.0
33	37.188605611871495	39.0	35.0	41.0	32.0	41.0
34	37.10451935218169	39.0	35.0	41.0	32.0	41.0
35	36.984881495556316	39.0	35.0	41.0	31.0	41.0
36	36.967413322667575	39.0	35.0	41.0	31.0	41.0
37	36.86923327358467	39.0	35.0	41.0	31.0	41.0
38	36.77271957369321	39.0	35.0	41.0	31.0	41.0
39	36.819302951042154	39.0	35.0	41.0	31.0	41.0
40	36.77205788848546	39.0	35.0	41.0	31.0	41.0
41	36.74882819982049	39.0	35.0	41.0	31.0	41.0
42	36.64008334911915	39.0	35.0	41.0	31.0	41.0
43	36.539430159231856	39.0	35.0	40.0	31.0	41.0
44	36.46224568783338	39.0	35.0	40.0	30.0	41.0
45	36.423361392514934	39.0	35.0	40.0	30.0	41.0
46	36.404767193921785	39.0	35.0	40.0	30.0	41.0
47	36.28776130365705	39.0	35.0	40.0	30.0	41.0
48	36.23557365310916	39.0	35.0	40.0	30.0	41.0
49	36.231481652482294	39.0	35.0	40.0	30.0	41.0
50	36.10958119125499	39.0	35.0	40.0	30.0	41.0
51	35.11881787352104	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	10.0
10	8.0
11	18.0
12	21.0
13	15.0
14	24.0
15	56.0
16	72.0
17	170.0
18	371.0
19	662.0
20	1245.0
21	2027.0
22	3229.0
23	5054.0
24	7383.0
25	11477.0
26	15776.0
27	18494.0
28	20546.0
29	23366.0
30	28538.0
31	36674.0
32	48303.0
33	67553.0
34	115214.0
35	152965.0
36	155598.0
37	241664.0
38	439631.0
39	498759.0
40	233.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.44825795803101	23.612083617170256	26.457277244519066	10.48238118027967
2	32.56240498828332	23.692498948638136	26.16368741230956	17.581408650768985
3	28.444549038313895	24.2205279656979	30.27969655348543	17.055226442502775
4	26.465719799003885	25.87358013382504	29.36325726415856	18.297442803012515
5	23.256915903187117	29.27144448413618	28.436159249794606	19.035480362882097
6	24.611312706413862	32.64424500082051	27.089730107362907	15.654712185402717
7	77.87802724939992	5.271742084181766	11.039378712415463	5.810851954002852
8	77.69561530656235	3.738204827980314	11.049879139555953	7.516300725901388
9	72.24789872733768	5.7183004504630475	14.019758743452403	8.014042078746872
10	37.59611980195878	23.500905727798322	22.378943002731695	16.524031467511204
11	26.899614333557942	24.206808814660075	27.874623844623226	21.018953007158757
12	24.362310115077292	21.545715641045803	33.025795697568704	21.066178546308205
13	22.27610213591352	25.094068525048797	34.773984901546626	17.855844437491065
14	18.888790978708407	28.76378312977103	31.326362245740597	21.021063645779964
15	16.419713153658186	26.48920065366478	37.60683129296139	19.484254899715644
16	18.81512969082838	25.648322226977022	33.53488173300316	22.00166634919144
17	18.778668408647075	26.29428317699657	29.6442887965719	25.28275961778445
18	20.431878874670804	25.990192917646578	32.63200329681752	20.945924910865095
19	20.244348633176813	28.91775421718788	29.516489628057986	21.321407521577324
20	20.79322020662097	28.35532179060249	29.9047416024285	20.946716400348045
21	19.89239964309101	29.423832592587118	30.668687251373367	20.015080512948504
22	18.446928783359304	27.08561436205156	27.67912594233419	26.78833091225495
23	17.253415409033853	27.927231512256746	29.259519375926374	25.559833702783035
24	20.111536697937538	24.51111013787219	31.476639715570336	23.900713448619932
25	17.68103079368982	28.18736772232016	28.444285208486246	25.68731627550377
26	16.884950671737123	29.687556888306588	30.23758931299241	23.189903126963884
27	19.78929494644518	27.748882548764986	28.685267373062235	23.776555131727594
28	17.787723575991695	27.177005014349703	29.97275693199681	25.06251447766179
29	18.273223224834194	27.874465546726633	28.824305692234063	25.028005536205107
30	20.15311627877526	27.267182049440652	28.008807694966286	24.5708939768178
31	20.240655015589702	28.825466543475724	24.91556126366045	26.01831717727412
32	19.59611874663947	30.62710767053564	26.106647403571515	23.670126179253373
33	20.593395495158457	26.762633887041787	27.068993082909575	25.574977534890174
34	19.135049739837406	26.064645695009553	28.309151570763646	26.491152994389395
35	17.406806070829866	27.570586351238763	28.18335750893987	26.8392500689915
36	22.128199134532633	25.91289077814497	27.825393198783637	24.133516888538757
37	20.821502764145105	26.722848349032084	28.713549930586375	23.742098956236436
38	19.098535691690575	28.01936088807231	29.247013842095736	23.635089578141383
39	21.777885889378265	24.13942667667813	30.495456586538026	23.58723084740558
40	19.840636230905975	23.31759676354674	32.22818536261563	24.613581642931656
41	18.568448801975133	24.769927198797358	28.94941379650594	27.712210202721565
42	22.79146732124606	23.917387493727446	28.734234189074176	24.55691099595232
43	23.161409505577627	24.10333475625554	28.652974602157812	24.082281136009026
44	20.96914193569834	25.034548515930837	27.032584566693806	26.963724981677018
45	20.37341418486345	23.53325126466828	28.106160901369332	27.98717364909894
46	21.639058634068554	23.996694739919196	28.99284018613722	25.371406439875027
47	18.598208806534114	24.13451944188383	31.68733421593205	25.579937535650004
48	19.33012551440221	21.928005061311413	31.589981009529005	27.151888414757373
49	22.4180425831895	20.34339035047682	32.01263639342515	25.225930672908532
50	19.550687250318045	20.21859884199812	31.734137627357256	28.49657628032658
51	18.743473509638495	20.04389073012794	28.319388168076486	32.893247592157074
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	371.0
1	656.5
2	942.0
3	6409.5
4	11877.0
5	8328.5
6	4780.0
7	4834.5
8	4889.0
9	4979.5
10	5070.0
11	5111.0
12	5152.0
13	5242.5
14	5333.0
15	5309.5
16	5286.0
17	5149.5
18	5013.0
19	5289.5
20	5566.0
21	6041.5
22	6517.0
23	7102.0
24	7687.0
25	8512.0
26	9954.5
27	10572.0
28	14242.0
29	17912.0
30	20142.5
31	22373.0
32	26896.5
33	31420.0
34	34490.5
35	37561.0
36	41861.5
37	46162.0
38	53074.0
39	59986.0
40	72190.0
41	84394.0
42	97508.0
43	110622.0
44	133702.5
45	156783.0
46	189873.0
47	222963.0
48	256774.0
49	290585.0
50	275444.5
51	260304.0
52	207302.5
53	154301.0
54	124358.0
55	94415.0
56	77702.5
57	60990.0
58	51736.5
59	42483.0
60	39620.0
61	36757.0
62	32940.5
63	29124.0
64	23772.5
65	18421.0
66	14869.0
67	11317.0
68	8938.5
69	6560.0
70	5268.0
71	3976.0
72	3704.0
73	3432.0
74	2693.0
75	1417.0
76	880.0
77	696.5
78	513.0
79	405.0
80	297.0
81	250.5
82	204.0
83	130.0
84	56.0
85	33.0
86	10.0
87	9.0
88	8.0
89	5.5
90	3.0
91	2.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1895161.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.51059853236899
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.57359910675223	19.941373546266128
2	13.33497700397476	7.8704630560534365
3	5.877024939000335	5.203035706186777
4	3.229536427113037	3.8122221178477673
5	2.0098896719092987	2.965652360103508
6	1.3353827168759764	2.3644765946874666
7	0.9520029480023671	1.9665923760090724
8	0.7106801853668268	1.6778078108216001
9	0.5636284292834106	1.4969711068211211
>10	3.4144711196700746	20.106340144654595
>50	0.7097404175887028	15.04322723384887
>100	0.2763489015807375	11.696367861037194
>500	0.007994254954642437	1.6329624566559466
>1k	0.0038154398647157087	1.971339986993341
>5k	7.267504504220398E-4	1.5696406592659191
>10k+	1.8168761260550995E-4	0.6815269827473647
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC	12711	0.6707081878531692	No Hit
CCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC	9350	0.49336177770648515	No Hit
CTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGCT	7202	0.38002048374781877	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7092	0.37421622753950723	No Hit
TCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC	5631	0.2971251519000233	No Hit
CGCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTG	4216	0.22246131067492417	No Hit
ACTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC	3729	0.1967642854617629	TruSeq Adapter, Index 13 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTG	2767	0.14600342662180155	No Hit
AGCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTG	2419	0.12764087061732488	No Hit
GCCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTG	2384	0.12579406182377117	No Hit
GGCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTG	2047	0.10801193144012566	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTC	2018	0.10648171843975261	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCC	2002	0.10563746299127094	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5829789659031608E-4	0.0	0.0	0.3985413376488858	0.0
2	1.5829789659031608E-4	0.0	0.0	2.155542457870334	0.0
3	1.5829789659031608E-4	0.0	0.0	3.234395389098868	0.0
4	1.5829789659031608E-4	0.0	0.0	4.344274708059104	0.0
5	1.5829789659031608E-4	0.0	0.0	7.234266640142975	0.0
6	1.5829789659031608E-4	0.0	0.0	9.059705217656969	0.0
7	1.5829789659031608E-4	0.0	0.0	10.813065486256841	0.0
8	1.5829789659031608E-4	0.0	0.0	13.751127212938638	0.0
9	1.5829789659031608E-4	0.0	0.0	14.875464406454121	0.0
10	1.5829789659031608E-4	0.0	0.0	17.341481805503594	0.0
11	1.5829789659031608E-4	0.0	0.0	20.736655091572693	0.0
12	1.5829789659031608E-4	0.0	0.0	23.113709072738413	0.0
13	1.5829789659031608E-4	0.0	0.0	24.11953390767328	0.0
14	1.5829789659031608E-4	0.0	0.0	24.50477822200858	0.0
15	1.5829789659031608E-4	0.0	0.0	25.144037894405805	0.0
16	1.5829789659031608E-4	0.0	0.0	26.705699410234804	0.0
17	2.1106386212042143E-4	0.0	0.0	28.687008649924728	0.0
18	2.1106386212042143E-4	0.0	0.0	30.72182257866218	0.0
19	2.1106386212042143E-4	0.0	0.0	31.91839637898838	0.0
20	2.6382982765052677E-4	0.0	0.0	33.03956761457206	0.0
21	2.6382982765052677E-4	0.0	0.0	34.522660607726735	0.0
22	2.6382982765052677E-4	0.0	0.0	36.037413180199465	0.0
23	2.6382982765052677E-4	0.0	0.0	37.651365767868796	0.0
24	2.6382982765052677E-4	0.0	0.0	38.82577786267235	0.0
25	2.6382982765052677E-4	0.0	0.0	39.87529291706615	0.0
26	3.1659579318063215E-4	0.0	0.0	40.843179022784874	0.0
27	3.1659579318063215E-4	0.0	0.0	41.756610652076525	0.0
28	3.1659579318063215E-4	0.0	0.0	42.684763985751076	0.0
29	3.1659579318063215E-4	0.0	0.0	43.6608815820925	0.0
30	3.693617587107375E-4	0.0	0.0	44.684646845307604	0.0
31	4.2212772424084287E-4	0.0	0.0	45.68746402020725	0.0
32	4.2212772424084287E-4	0.0	0.0	46.634138207782875	0.0
33	4.2212772424084287E-4	0.0	0.0	47.51923451358486	0.0
34	4.2212772424084287E-4	0.0	0.0	48.32776740340267	0.0
35	4.2212772424084287E-4	0.0	0.0	49.20605689965127	0.0
36	4.2212772424084287E-4	0.0	0.0	50.023771067471316	0.0
37	4.2212772424084287E-4	0.0	0.0	50.860006089192424	0.0
38	4.2212772424084287E-4	0.0	0.0	51.66468706352653	0.0
39	4.2212772424084287E-4	0.0	0.0	52.41802675339984	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	20	7.034446E-4	45.000004	26
GCGCGAA	25	3.8917668E-5	45.000004	17
CCGTGAT	20	7.034446E-4	45.000004	33
CCTACGG	20	7.034446E-4	45.000004	2
CACGAAA	25	3.8917668E-5	45.000004	42
TCGGCTA	20	7.034446E-4	45.000004	39
CGTTAGA	20	7.034446E-4	45.000004	22
CCCGTAG	20	7.034446E-4	45.000004	14
AACGCGC	20	7.034446E-4	45.000004	40
GTCGTAC	20	7.034446E-4	45.000004	8
TCCGTCA	25	3.8917668E-5	45.000004	29
CGTCGTC	25	3.8917668E-5	45.000004	34
ACCGTCG	20	7.034446E-4	45.000004	19
CGCAATT	25	3.8917668E-5	45.000004	31
CGACTCG	20	7.034446E-4	45.000004	2
CGCAACT	20	7.034446E-4	45.000004	42
TGTACCG	20	7.034446E-4	45.000004	2
TATCGCA	25	3.8917668E-5	45.000004	28
ATACCGG	50	2.1827873E-11	45.000004	2
GCGTAAT	20	7.034446E-4	45.000004	9
>>END_MODULE