##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934750.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1972514
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36925669475603	33.0	31.0	34.0	31.0	34.0
2	32.53995408904576	34.0	31.0	34.0	31.0	34.0
3	32.5431657265804	34.0	31.0	34.0	31.0	34.0
4	36.06416937978641	37.0	35.0	37.0	35.0	37.0
5	35.9616322114824	37.0	35.0	37.0	35.0	37.0
6	35.89087022956491	37.0	35.0	37.0	35.0	37.0
7	36.14231533971368	37.0	35.0	37.0	35.0	37.0
8	35.93000556650041	37.0	35.0	37.0	35.0	37.0
9	37.6016073903658	39.0	38.0	39.0	35.0	39.0
10	37.461474037700114	39.0	37.0	39.0	35.0	39.0
11	37.371478225249604	39.0	37.0	39.0	35.0	39.0
12	37.24812193981893	39.0	37.0	39.0	34.0	39.0
13	37.171329582451634	39.0	37.0	39.0	34.0	39.0
14	38.39449098967105	40.0	38.0	41.0	34.0	41.0
15	38.4625320783528	40.0	38.0	41.0	34.0	41.0
16	38.563324772346355	40.0	38.0	41.0	35.0	41.0
17	38.527119706121226	40.0	38.0	41.0	34.0	41.0
18	38.51471269658923	40.0	38.0	41.0	35.0	41.0
19	38.47511094978287	40.0	37.0	41.0	34.0	41.0
20	38.42137495602059	40.0	37.0	41.0	34.0	41.0
21	38.36633402855443	40.0	37.0	41.0	34.0	41.0
22	38.3182456499675	40.0	37.0	41.0	34.0	41.0
23	38.14697538268423	40.0	37.0	41.0	34.0	41.0
24	37.92370396357136	40.0	36.0	41.0	34.0	41.0
25	37.862762444271624	40.0	36.0	41.0	33.0	41.0
26	37.982822935604005	40.0	36.0	41.0	34.0	41.0
27	38.030298897751805	40.0	37.0	41.0	34.0	41.0
28	37.98426475046565	40.0	36.0	41.0	34.0	41.0
29	37.984421910313436	40.0	36.0	41.0	34.0	41.0
30	37.879368663543076	40.0	36.0	41.0	34.0	41.0
31	37.79756645580209	40.0	36.0	41.0	33.0	41.0
32	37.70426623081002	40.0	36.0	41.0	33.0	41.0
33	37.57865850381797	40.0	36.0	41.0	33.0	41.0
34	37.53019192766185	40.0	36.0	41.0	33.0	41.0
35	37.44097684477778	40.0	36.0	41.0	33.0	41.0
36	37.36233405694459	40.0	35.0	41.0	33.0	41.0
37	37.304910383399054	40.0	35.0	41.0	33.0	41.0
38	37.20983323819248	40.0	35.0	41.0	33.0	41.0
39	37.22221591329643	40.0	35.0	41.0	33.0	41.0
40	37.10667503500609	39.0	35.0	41.0	32.0	41.0
41	37.03514550467069	39.0	35.0	41.0	32.0	41.0
42	36.95863755593116	39.0	35.0	41.0	32.0	41.0
43	36.916876635603096	39.0	35.0	41.0	32.0	41.0
44	36.8647380956485	39.0	35.0	41.0	32.0	41.0
45	36.81508572309246	39.0	35.0	41.0	32.0	41.0
46	36.751918617561145	39.0	35.0	41.0	32.0	41.0
47	36.63386318170619	39.0	35.0	41.0	31.0	41.0
48	36.56872397356876	39.0	35.0	41.0	31.0	41.0
49	36.53979236649271	39.0	35.0	41.0	31.0	41.0
50	36.42884714633204	39.0	35.0	40.0	31.0	41.0
51	35.48909716230151	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	22.0
10	29.0
11	27.0
12	28.0
13	34.0
14	38.0
15	64.0
16	118.0
17	233.0
18	384.0
19	702.0
20	1375.0
21	2084.0
22	3151.0
23	4514.0
24	6450.0
25	8776.0
26	12262.0
27	14990.0
28	17265.0
29	19643.0
30	23916.0
31	30420.0
32	40405.0
33	58691.0
34	114122.0
35	159181.0
36	143880.0
37	224652.0
38	407640.0
39	676920.0
40	492.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.52943908129423	21.55949209992933	24.52803883774716	12.383029981029285
2	31.21960097621614	24.933156367964944	26.056849279650233	17.790393376168687
3	31.654832361139135	24.345581324137626	27.162189976851874	16.837396337871365
4	28.910010271156505	25.82653405755295	26.95463758432133	18.308818086969218
5	26.657858955627184	29.063012987487035	24.782789881339244	19.496338175546533
6	26.791850400047856	34.62606602538689	23.37620924363528	15.20587433092997
7	80.28419570152607	5.8627213799243	9.00896013919293	4.8441227793567
8	80.74705680162472	5.584751236239641	8.191931717594906	5.476260244540724
9	74.7399511486357	7.34149415416063	10.421269506832399	7.497285190371272
10	41.59230302040949	26.15824272983614	17.298990019842698	14.950464229911676
11	31.28043704632768	25.490110589836117	23.67567479875935	19.553777565076853
12	29.390564528312602	21.23016617372551	28.70250857535105	20.67676072261084
13	24.61858318876317	24.22426406099019	30.673140976439207	20.48401177380744
14	20.033672764806738	28.525627701501737	28.439848842644462	23.00085069104706
15	18.73147668406916	25.60225174574173	34.73465841053599	20.93161315965311
16	21.393815202325563	25.017617111969802	31.326368279261896	22.262199406442743
17	20.8065443388488	25.30385082184461	28.424082161140557	25.465522678166035
18	21.902962412434082	25.452949890342985	29.72354061872311	22.920547078499823
19	23.952276130866498	26.199712650962176	27.36406433617201	22.483946881999316
20	26.496491279656315	25.130721505652176	28.035136886227424	20.337650328464083
21	23.946699491106273	26.456998530808907	27.780639326260804	21.815662651824017
22	22.915578799440713	25.06598178770848	26.942723853924484	25.075715558926326
23	23.019963356407104	26.225162406958834	27.209287234463233	23.545587002170834
24	23.074158155531467	23.92256784996203	28.92425605090762	24.079017943598878
25	21.71933887414741	26.14982707347071	27.81840838645505	24.31242566592683
26	21.584891159200897	27.40218827344191	28.166745584568726	22.84617498278846
27	22.95228322840801	27.461604835250853	27.21602989890059	22.370082037440543
28	20.55792759899296	27.03935181195165	29.519587693674165	22.883132895381227
29	22.65844500976926	24.94375198350937	29.48876408481765	22.909038921903722
30	23.992225150239747	25.742225403723367	27.57374599115646	22.69180345488042
31	24.03095744821076	26.532181774121753	26.342119751748278	23.09474102591921
32	25.71424081147206	27.327005030129065	25.994543004510994	20.96421115388788
33	24.72225799157826	27.06936427320668	25.46881796529708	22.73955976991798
34	23.59750044866602	25.528842887807134	28.201016570731564	22.672640092795284
35	23.887840593273353	25.43145447890357	27.063939723621733	23.61676520420134
36	23.900210594196036	27.33917224415137	26.507289682101113	22.253327479551476
37	24.872827265104327	27.020999597468006	27.568625621922077	20.537547515505594
38	22.6466326728226	28.060586642224084	25.75297310944308	23.53980757551024
39	24.614578147480827	25.510845550399136	26.860037495297878	23.014538806822156
40	24.200132419845943	25.050113712754385	29.643389096351154	21.10636477104852
41	21.288974374833334	27.00533430941428	27.251365516290377	24.454325799462005
42	22.38306039906434	25.96635562535931	27.824035722940373	23.826548252635977
43	23.945634859879323	24.433895019249547	27.8410698225716	23.77940029829953
44	22.783564527298665	24.935741900944684	28.32765699001376	23.953036581742893
45	22.80460366821224	24.632169911088084	27.299882282204337	25.26334413849534
46	23.81473591568932	25.82323877042191	27.37395019756514	22.988075116323635
47	22.100679640296597	25.329148487665993	29.425241088276177	23.144930783761232
48	22.479029299665303	23.731643983261968	28.826005797677485	24.963320919395247
49	22.229753502383254	23.551467822281616	30.215603032475308	24.003175642859823
50	21.14382965089221	24.041755850655562	29.941283052997342	24.873131445454884
51	21.069356161730664	24.27151340877682	27.223127440413602	27.43600298907891
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	404.0
1	1142.0
2	1880.0
3	6017.5
4	10155.0
5	7192.5
6	4230.0
7	4155.0
8	4080.0
9	4010.0
10	3940.0
11	3918.0
12	3896.0
13	3824.0
14	3752.0
15	3798.0
16	3844.0
17	3565.5
18	3287.0
19	3468.0
20	3649.0
21	4471.5
22	5294.0
23	5857.5
24	6421.0
25	8584.0
26	13608.5
27	16470.0
28	20051.5
29	23633.0
30	28406.5
31	33180.0
32	35990.0
33	38800.0
34	43395.5
35	47991.0
36	52614.0
37	57237.0
38	61155.0
39	65073.0
40	75181.5
41	85290.0
42	93812.0
43	102334.0
44	115620.0
45	128906.0
46	153131.0
47	177356.0
48	188494.0
49	199632.0
50	193228.0
51	186824.0
52	161356.0
53	135888.0
54	121622.0
55	107356.0
56	102522.0
57	97688.0
58	94538.0
59	91388.0
60	89995.0
61	88602.0
62	79842.0
63	71082.0
64	62281.5
65	53481.0
66	45458.0
67	37435.0
68	29641.0
69	21847.0
70	19134.5
71	16422.0
72	13170.0
73	9918.0
74	7947.0
75	4570.0
76	3164.0
77	2783.0
78	2402.0
79	1650.5
80	899.0
81	652.0
82	405.0
83	281.5
84	158.0
85	98.5
86	39.0
87	39.5
88	40.0
89	24.0
90	8.0
91	6.5
92	5.0
93	5.5
94	6.0
95	3.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1972514.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.182218223255255
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.64710546841809	13.652686447351778
2	12.609596176691925	5.089792434902431
3	5.558099353875809	3.3652432219936683
4	2.9221314586547225	2.359003791024352
5	1.7612240590088288	1.7772704149481788
6	1.1716670732047205	1.4188104333852276
7	0.8040647483958562	1.1359467152426401
8	0.603684709341566	0.9746957233579123
9	0.4452148961220441	0.8086881770801113
>10	3.775040832476468	17.87612788220402
>50	1.9533910482847843	29.12971565548988
>100	0.738039476706474	18.945900387597685
>500	0.006137542182116882	0.8032918636453527
>1k	0.0038359638638230512	1.6925179956644856
>5k	7.671927727646102E-4	0.9703088561123511
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	6982	0.353964534598994	TruSeq Adapter, Index 15 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	6428	0.3258785488974983	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5390	0.27325534825101366	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGC	4752	0.24091083764171004	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCG	4224	0.21414296679263112	No Hit
CTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGCT	4188	0.21231788468928484	TruSeq Adapter, Index 14 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTC	3727	0.18894669442143375	No Hit
TCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	2160	0.10950492620077727	TruSeq Adapter, Index 15 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.069672509295245E-5	0.0	0.0	0.22189956573185285	0.0
2	5.069672509295245E-5	0.0	0.0	1.1441743886228437	0.0
3	5.069672509295245E-5	0.0	0.0	1.621331965197712	0.0
4	1.013934501859049E-4	0.0	0.0	2.1425449958783562	0.0
5	1.013934501859049E-4	0.0	0.0	3.6270971967752828	0.0
6	1.013934501859049E-4	0.0	0.0	4.3736571704941	0.0
7	1.013934501859049E-4	0.0	0.0	5.207719691723354	0.0
8	1.013934501859049E-4	0.0	0.0	6.518534215726732	0.0
9	1.013934501859049E-4	0.0	0.0	6.964665396544714	0.0
10	1.013934501859049E-4	0.0	0.0	8.179612413397319	0.0
11	1.013934501859049E-4	0.0	0.0	9.56479903311206	0.0
12	1.013934501859049E-4	0.0	0.0	10.864257490694616	0.0
13	1.013934501859049E-4	0.0	0.0	11.291326702877647	0.0
14	1.013934501859049E-4	0.0	0.0	11.458220321883648	0.0
15	1.013934501859049E-4	0.0	0.0	11.827698054361084	0.0
16	1.013934501859049E-4	0.0	0.0	12.549619419684728	0.0
17	1.013934501859049E-4	0.0	0.0	13.40548153270395	0.0
18	1.013934501859049E-4	0.0	0.0	14.317464920401072	0.0
19	1.013934501859049E-4	0.0	0.0	14.944431319625615	0.0
20	1.013934501859049E-4	0.0	0.0	15.480802671109052	0.0
21	1.013934501859049E-4	0.0	0.0	16.174181780205362	0.0
22	1.013934501859049E-4	0.0	0.0	16.938739091332177	0.0
23	1.013934501859049E-4	0.0	0.0	17.664817588113443	0.0
24	1.013934501859049E-4	0.0	0.0	18.24889455790935	0.0
25	1.013934501859049E-4	0.0	0.0	18.757940374567685	0.0
26	1.013934501859049E-4	0.0	0.0	19.229977581908162	0.0
27	1.013934501859049E-4	0.0	0.0	19.695880485512397	0.0
28	1.013934501859049E-4	0.0	0.0	20.157068593682986	0.0
29	1.013934501859049E-4	0.0	0.0	20.648877523809716	0.0
30	1.013934501859049E-4	0.0	0.0	21.19736539259037	0.0
31	2.027869003718098E-4	0.0	0.0	21.756398180190356	0.0
32	2.534836254647622E-4	0.0	0.0	22.279943260225277	0.0
33	2.534836254647622E-4	0.0	0.0	22.7746926004074	0.0
34	2.534836254647622E-4	0.0	0.0	23.25686915276647	0.0
35	2.534836254647622E-4	0.0	0.0	23.753899845577774	0.0
36	2.534836254647622E-4	0.0	0.0	24.272121769477934	0.0
37	2.534836254647622E-4	0.0	0.0	24.77822717608088	0.0
38	2.534836254647622E-4	0.0	0.0	25.270948647259285	0.0
39	2.534836254647622E-4	0.0	0.0	25.772491348603864	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATATCG	35	1.2125201E-7	45.000004	1
CACTACG	30	2.1662963E-6	45.000004	18
ACGTGCG	30	2.1662963E-6	45.000004	1
TCGCAAT	25	3.891826E-5	45.0	30
CGAAACG	20	7.034517E-4	45.0	33
CGACGAA	20	7.034517E-4	45.0	19
TAACGCC	20	7.034517E-4	45.0	12
TATGCGA	20	7.034517E-4	45.0	14
TTATCCG	40	6.8193913E-9	45.0	1
CGTTATG	20	7.034517E-4	45.0	8
ATCGACT	20	7.034517E-4	45.0	21
TATACGG	130	0.0	41.538464	2
TCGACAG	100	0.0	40.5	1
ACGCACT	45	1.929584E-8	40.0	12
CGTTTTT	3445	0.0	39.579098	1
AACGGGC	300	0.0	39.0	4
GAACGTA	35	6.250564E-6	38.57143	37
ATAGCGG	345	0.0	38.47826	2
AATAGCG	65	9.094947E-12	38.076927	1
TACGGGA	630	0.0	37.857143	4
>>END_MODULE