##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934748.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1780701
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31000656483037	33.0	31.0	34.0	30.0	34.0
2	32.490952720305096	34.0	31.0	34.0	31.0	34.0
3	32.493236652307154	34.0	31.0	34.0	31.0	34.0
4	36.00655865302485	37.0	35.0	37.0	35.0	37.0
5	35.90319205750993	37.0	35.0	37.0	35.0	37.0
6	35.829870371275135	37.0	35.0	37.0	35.0	37.0
7	36.0935468672169	37.0	35.0	37.0	35.0	37.0
8	35.918418083664804	37.0	35.0	37.0	35.0	37.0
9	37.56239144022494	39.0	38.0	39.0	35.0	39.0
10	37.37625856334107	39.0	37.0	39.0	34.0	39.0
11	37.27198895266527	39.0	37.0	39.0	34.0	39.0
12	37.12114049467036	39.0	37.0	39.0	34.0	39.0
13	37.034694763466746	39.0	37.0	39.0	33.0	39.0
14	38.20242533698807	40.0	37.0	41.0	33.0	41.0
15	38.27668036351976	40.0	37.0	41.0	34.0	41.0
16	38.369762806894585	40.0	37.0	41.0	34.0	41.0
17	38.34882891625264	40.0	37.0	41.0	34.0	41.0
18	38.32817693706018	40.0	37.0	41.0	34.0	41.0
19	38.3023960788476	40.0	37.0	41.0	34.0	41.0
20	38.26016945012105	40.0	37.0	41.0	34.0	41.0
21	38.17116798384456	40.0	37.0	41.0	34.0	41.0
22	38.11050648031309	40.0	36.0	41.0	34.0	41.0
23	37.96244288064083	40.0	36.0	41.0	34.0	41.0
24	37.711597848263125	40.0	36.0	41.0	33.0	41.0
25	37.63644710706626	40.0	35.0	41.0	33.0	41.0
26	37.75443322601605	40.0	36.0	41.0	33.0	41.0
27	37.7984400525411	40.0	36.0	41.0	33.0	41.0
28	37.729730033284646	40.0	36.0	41.0	33.0	41.0
29	37.70953293113218	40.0	36.0	41.0	33.0	41.0
30	37.57364543514043	40.0	35.0	41.0	33.0	41.0
31	37.48765345782363	40.0	35.0	41.0	33.0	41.0
32	37.40563968908874	40.0	35.0	41.0	33.0	41.0
33	37.284780544291266	39.0	35.0	41.0	33.0	41.0
34	37.18797316337779	39.0	35.0	41.0	32.0	41.0
35	37.0937737441603	39.0	35.0	41.0	32.0	41.0
36	37.03037230843359	39.0	35.0	41.0	32.0	41.0
37	36.95550179395642	39.0	35.0	41.0	32.0	41.0
38	36.869190279558445	39.0	35.0	41.0	31.0	41.0
39	36.89656152268124	39.0	35.0	41.0	32.0	41.0
40	36.80757016478342	39.0	35.0	41.0	31.0	41.0
41	36.72525145995875	39.0	35.0	41.0	31.0	41.0
42	36.620548312153474	39.0	35.0	41.0	31.0	41.0
43	36.54502917671187	39.0	35.0	41.0	31.0	41.0
44	36.47111446559529	39.0	35.0	41.0	31.0	41.0
45	36.398616050645224	39.0	35.0	41.0	31.0	41.0
46	36.32857228698136	38.0	35.0	40.0	31.0	41.0
47	36.204901889761395	38.0	35.0	40.0	31.0	41.0
48	36.14219512427971	38.0	35.0	40.0	31.0	41.0
49	36.108091700964955	38.0	35.0	40.0	31.0	41.0
50	36.00033076861303	38.0	35.0	40.0	31.0	41.0
51	35.027173006585606	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	25.0
10	53.0
11	26.0
12	28.0
13	32.0
14	38.0
15	68.0
16	124.0
17	215.0
18	413.0
19	711.0
20	1279.0
21	2064.0
22	3028.0
23	4397.0
24	6259.0
25	8702.0
26	11990.0
27	15045.0
28	17334.0
29	20183.0
30	24976.0
31	31768.0
32	41620.0
33	59558.0
34	116119.0
35	156019.0
36	140879.0
37	215219.0
38	367988.0
39	534150.0
40	384.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.1956869794536	22.22001335429137	25.49383641610804	12.090463250146993
2	31.80983219529837	24.899407592852477	25.177444163843337	18.113316048005814
3	31.893282476957108	23.75884553330402	27.08085186676483	17.267020122974042
4	28.888117657035068	25.904124274653633	26.303292916665967	18.904465151645336
5	26.10219233885981	29.57200563149007	24.462107900203346	19.863694129446774
6	26.88817493784751	34.31339680271982	23.568190280120017	15.23023797931264
7	80.84568942231178	6.098721795517608	8.042057594172183	5.013531187998434
8	82.04476776280801	4.641205907111862	8.176779818734307	5.137246511345812
9	75.89859274521663	7.120566563392732	10.42100835569812	6.5598323356925174
10	43.009803442576825	24.99717807762224	17.08074516721224	14.912273312588695
11	32.72430351867045	24.625526688646772	23.226414765870295	19.423755026812476
12	30.31362367966323	20.853585189203578	28.011271965366447	20.82151916576674
13	25.116007684614093	23.531407013305433	29.78518010603689	21.56740519604358
14	20.818767440463056	26.406791482680138	29.41246172153551	23.361979355321303
15	20.349963300969677	24.374558109418707	34.25353273794983	21.021945851661787
16	22.58902533328167	23.474687777453934	31.94562141538641	21.990665473877986
17	22.842689480154164	23.58200506429771	28.615135275377508	24.960170180170618
18	23.57897255069773	24.537303005951028	29.07287635599688	22.810848087354362
19	24.89457803415621	25.803995168194998	26.695554166589453	22.60587263105934
20	27.100113943890637	25.975051398297637	26.02171841314179	20.903116244669935
21	25.189574218243266	26.23590372555527	27.08180654697223	21.492715509229228
22	23.64080213354179	24.714705051549924	26.682188643685826	24.962304171222456
23	24.310089116589477	25.33957132612381	26.861668522677306	23.488671034609403
24	24.253650669034275	23.053280702375076	28.540389430903897	24.152679197686755
25	22.968089533279308	24.807421346986384	26.35507027850268	25.869418841231628
26	22.8615022960059	26.40954320798382	27.442226404095916	23.286728091914362
27	23.338842399706632	26.522139314797936	27.032724752779945	23.106293532715487
28	20.791643291041	27.212990839001044	27.796188130404826	24.199177739553132
29	23.65119130050469	25.247528922598462	27.29672190895608	23.80455786794077
30	23.537584355823917	25.80635378988387	27.250167209430444	23.40589464486177
31	23.95646433623612	26.534044738560823	24.941862783252216	24.56762814195084
32	25.294757514035204	27.740704363057024	23.8822800683551	23.082258054552675
33	23.923275159614107	27.08708536694257	25.240902318805908	23.748737154637414
34	22.03531081298882	25.82595281296523	26.866329608395795	25.272406765650157
35	22.32525280774257	27.55847275876186	25.77962274407663	24.336651689418943
36	22.715436224273475	28.09500303532148	25.28346982452416	23.90609091588088
37	22.956521055471974	28.172163659143223	25.043788934807136	23.827526350577667
38	21.030987234802474	28.941972852264364	25.09101752624388	24.936022386689288
39	23.139370394018986	25.349735862449673	26.399715617613513	25.111178125917828
40	23.35866605342503	24.475080319492154	28.06681189037351	24.099441736709306
41	20.656191016908508	26.741154185907686	26.08203173918586	26.520623057997945
42	22.28757101837984	25.654110375632968	25.819887785765268	26.238430820221925
43	22.686908133369947	25.350241281383006	26.485075259687058	25.477775325559993
44	21.91468416090068	25.645630569084872	26.033567679245422	26.40611759076903
45	22.200414331210013	24.49080446408465	26.521353107568313	26.787428097137028
46	22.898173247501965	25.754071009113826	26.39730083826538	24.95045490511883
47	20.942482763810432	25.830389268046684	27.839204897397146	25.38792307074573
48	21.2079400191273	24.35580145122623	28.20220800684674	26.23405052279973
49	21.940067422885708	23.67634993185268	29.004139381063975	25.379443264197636
50	20.54230328393144	24.16986344141998	28.397187399793676	26.8906458748549
51	19.748065509032678	24.067375713272472	27.062712942824202	29.121845834870648
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	397.0
1	950.0
2	1503.0
3	5500.0
4	9497.0
5	6488.0
6	3479.0
7	3462.5
8	3446.0
9	3322.5
10	3199.0
11	3148.5
12	3098.0
13	3120.0
14	3142.0
15	3195.5
16	3249.0
17	3335.0
18	3421.0
19	3504.0
20	3587.0
21	3983.0
22	4379.0
23	4576.0
24	4773.0
25	6356.0
26	9940.0
27	11941.0
28	15915.0
29	19889.0
30	22015.5
31	24142.0
32	27997.0
33	31852.0
34	35485.5
35	39119.0
36	42938.5
37	46758.0
38	50417.5
39	54077.0
40	62447.0
41	70817.0
42	81768.0
43	92719.0
44	101359.5
45	110000.0
46	125519.0
47	141038.0
48	154384.5
49	167731.0
50	166824.5
51	165918.0
52	145936.5
53	125955.0
54	112123.5
55	98292.0
56	95583.5
57	92875.0
58	93430.5
59	93986.0
60	94138.5
61	94291.0
62	86438.5
63	78586.0
64	66919.0
65	55252.0
66	46529.5
67	37807.0
68	32204.0
69	26601.0
70	22203.0
71	17805.0
72	14990.0
73	12175.0
74	9258.0
75	5357.5
76	4374.0
77	3453.5
78	2533.0
79	2165.0
80	1797.0
81	1130.0
82	463.0
83	355.0
84	247.0
85	165.0
86	83.0
87	90.0
88	97.0
89	56.5
90	16.0
91	11.0
92	6.0
93	5.0
94	4.0
95	3.5
96	3.0
97	1.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1780701.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.491933481416506
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.24906207120752	14.882962356848747
2	12.0123654764643	5.163379195492697
3	5.042672968128951	3.2513037599869365
4	2.7010948281397185	2.322070014935079
5	1.6226580357168778	1.7437029283356553
6	1.0993077807704004	1.4175749819952626
7	0.7281430561961512	1.09544414901061
8	0.5639143357058449	0.9695687513765757
9	0.4195188081522057	0.8114643287109304
>10	4.039199221015913	21.254327177939885
>50	2.0064962555665073	31.206921077342347
>100	0.5091857231347178	13.93695882054008
>500	0.004254293200678907	0.6476312723571379
>1k	0.0021271466003394534	1.2966911851280172
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTGC	4938	0.2773065214204967	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4876	0.27382474654644434	No Hit
GCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTGC	4611	0.25894296684283324	No Hit
CTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTGCT	2626	0.14747001321389724	TruSeq Adapter, Index 13 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.684729777767295E-4	0.0	0.0	0.15673602699161734	0.0
2	1.684729777767295E-4	0.0	0.0	0.896893976024049	0.0
3	1.684729777767295E-4	0.0	0.0	1.306676415636314	0.0
4	1.684729777767295E-4	0.0	0.0	1.7494234012335592	0.0
5	1.684729777767295E-4	0.0	0.0	2.9429982911224286	0.0
6	1.684729777767295E-4	0.0	0.0	3.6849532852511455	0.0
7	1.684729777767295E-4	0.0	0.0	4.493679736238706	0.0
8	1.684729777767295E-4	0.0	0.0	5.837869468259972	0.0
9	1.684729777767295E-4	0.0	0.0	6.342052933086464	0.0
10	1.684729777767295E-4	0.0	0.0	7.368165683065265	0.0
11	1.684729777767295E-4	0.0	0.0	8.803499296063741	0.0
12	1.684729777767295E-4	0.0	0.0	9.89200320547919	0.0
13	1.684729777767295E-4	0.0	0.0	10.329190582809803	0.0
14	1.684729777767295E-4	0.0	0.0	10.507322677979065	0.0
15	1.684729777767295E-4	0.0	0.0	10.854377012199128	0.0
16	1.684729777767295E-4	0.0	0.0	11.580944807690903	0.0
17	1.684729777767295E-4	0.0	0.0	12.419547133404205	0.0
18	1.684729777767295E-4	0.0	0.0	13.350360335620636	0.0
19	1.684729777767295E-4	0.0	0.0	13.889754652802464	0.0
20	1.684729777767295E-4	0.0	0.0	14.41174009561403	0.0
21	1.684729777767295E-4	0.0	0.0	15.067605398098838	0.0
22	1.684729777767295E-4	0.0	0.0	15.791084522331374	0.0
23	1.684729777767295E-4	0.0	0.0	16.47738727613451	0.0
24	1.684729777767295E-4	0.0	0.0	17.05289096821982	0.0
25	1.684729777767295E-4	0.0	0.0	17.54427048673528	0.0
26	1.684729777767295E-4	0.0	0.0	17.992240134643605	0.0
27	1.684729777767295E-4	0.0	0.0	18.425777264122388	0.0
28	1.684729777767295E-4	0.0	0.0	18.894581403615767	0.0
29	1.684729777767295E-4	0.0	0.0	19.349963862546268	0.0
30	1.684729777767295E-4	0.0	0.0	19.875599553209664	0.0
31	1.684729777767295E-4	0.0	0.0	20.385904202895375	0.0
32	1.684729777767295E-4	0.0	0.0	20.864929036373876	0.0
33	1.684729777767295E-4	0.0	0.0	21.33412627948207	0.0
34	2.2463063703563932E-4	0.0	0.0	21.797876229642146	0.0
35	2.2463063703563932E-4	0.0	0.0	22.26600647722442	0.0
36	2.2463063703563932E-4	0.0	0.0	22.756206684895442	0.0
37	2.2463063703563932E-4	0.0	0.0	23.253763545929385	0.0
38	2.2463063703563932E-4	0.0	0.0	23.75468986651886	0.0
39	2.2463063703563932E-4	0.0	0.0	24.256683182634255	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACAT	30	2.1661763E-6	45.000004	35
CGACCGT	20	7.034333E-4	45.0	26
CCGTCTA	25	3.8916725E-5	45.0	13
TACGCAC	20	7.034333E-4	45.0	30
CGTAGAT	20	7.034333E-4	45.0	34
CAATCGA	25	3.8916725E-5	45.0	45
GCGTCTA	20	7.034333E-4	45.0	33
AACACGT	140	0.0	41.785713	41
CGCAATA	55	6.184564E-11	40.909092	22
TATAGCG	85	0.0	39.705883	1
CGTTTTT	3185	0.0	39.41915	1
TAAGACG	70	0.0	38.571426	1
ACGACTA	35	6.250224E-6	38.571426	27
AAATGCG	135	0.0	38.333336	1
ATATGCG	60	1.5643309E-10	37.500004	1
CCGTAGT	30	1.1401738E-4	37.500004	32
GACGAAC	30	1.1401738E-4	37.500004	40
GTGCGAT	30	1.1401738E-4	37.500004	38
TCGAATA	30	1.1401738E-4	37.500004	20
TAGCGCG	140	0.0	36.964283	1
>>END_MODULE