SRR2934745.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934745.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2251139
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	10596	0.4706950570355718	RNA PCR Primer, Index 35 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	7697	0.3419158035110227	RNA PCR Primer, Index 10 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5865	0.26053477817229415	No Hit
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT	5801	0.25769177292028617	TruSeq Adapter, Index 10 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	4089	0.18164138242907257	RNA PCR Primer, Index 35 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	2767	0.12291555519228267	TruSeq Adapter, Index 10 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG	2312	0.1027035647287884	TruSeq Adapter, Index 10 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCGT	30	2.1664328E-6	45.000004	36
ACGACCG	30	2.1664328E-6	45.000004	1
ACGCGAA	25	3.8920025E-5	45.0	13
GGTCGAT	25	3.8920025E-5	45.0	42
GGCCGTA	20	7.034729E-4	45.0	10
CTGCGTA	25	3.8920025E-5	45.0	1
CCCGTTG	25	3.8920025E-5	45.0	19
TCGGTCG	20	7.034729E-4	45.0	14
TACGTAG	85	0.0	45.0	1
TATCCGT	35	1.2126293E-7	45.0	18
CGTAAGG	300	0.0	43.5	2
GATTACG	100	0.0	40.5	1
ATCGGGT	95	0.0	40.26316	4
TTAAGCG	90	0.0	40.0	1
CGTTTTT	3965	0.0	39.779324	1
ACGCATG	115	0.0	39.130432	1
TCGGTAC	70	0.0	38.571426	36
TACGGGA	740	0.0	38.006756	4
CATATCG	60	1.5643309E-10	37.500004	2
GTATACG	60	1.5643309E-10	37.500004	1