##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934745.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2251139
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22184280935118	33.0	31.0	34.0	30.0	34.0
2	32.40037065680973	34.0	31.0	34.0	30.0	34.0
3	32.40655730276984	34.0	31.0	34.0	30.0	34.0
4	35.94661591309999	37.0	35.0	37.0	35.0	37.0
5	35.843391278814856	37.0	35.0	37.0	35.0	37.0
6	35.77394199114315	37.0	35.0	37.0	35.0	37.0
7	36.09103702614543	37.0	35.0	37.0	35.0	37.0
8	35.93191224531226	37.0	35.0	37.0	35.0	37.0
9	37.64499882059704	39.0	38.0	39.0	35.0	39.0
10	37.35454141214736	39.0	37.0	39.0	34.0	39.0
11	37.23834378952166	39.0	37.0	39.0	34.0	39.0
12	37.14898946710976	39.0	37.0	39.0	34.0	39.0
13	37.093119527492526	39.0	37.0	39.0	33.0	39.0
14	38.24229734370024	40.0	37.0	41.0	33.0	41.0
15	38.3183983752225	40.0	38.0	41.0	34.0	41.0
16	38.389597443782904	40.0	38.0	41.0	34.0	41.0
17	38.38979512149183	40.0	38.0	41.0	34.0	41.0
18	38.37029876875662	40.0	37.0	41.0	34.0	41.0
19	38.35336822826134	40.0	37.0	41.0	34.0	41.0
20	38.323842286060525	40.0	37.0	41.0	34.0	41.0
21	38.23543281867535	40.0	37.0	41.0	34.0	41.0
22	38.17087838645237	40.0	37.0	41.0	34.0	41.0
23	37.96722325898134	40.0	36.0	41.0	34.0	41.0
24	37.71784194578833	40.0	36.0	41.0	33.0	41.0
25	37.631096524914724	39.0	36.0	41.0	33.0	41.0
26	37.773697670379306	40.0	36.0	41.0	33.0	41.0
27	37.86047640772071	40.0	36.0	41.0	34.0	41.0
28	37.78701137513055	40.0	36.0	41.0	33.0	41.0
29	37.81913999979566	40.0	36.0	41.0	33.0	41.0
30	37.73086202140339	40.0	36.0	41.0	33.0	41.0
31	37.62727445972905	40.0	36.0	41.0	33.0	41.0
32	37.569245168779005	40.0	36.0	41.0	33.0	41.0
33	37.4568691671194	40.0	36.0	41.0	33.0	41.0
34	37.39913661484253	40.0	36.0	41.0	33.0	41.0
35	37.375449938897596	40.0	36.0	41.0	33.0	41.0
36	37.342520830566215	40.0	36.0	41.0	33.0	41.0
37	37.28683701894907	40.0	35.0	41.0	33.0	41.0
38	37.207243088942974	39.0	35.0	41.0	32.0	41.0
39	37.20545110719507	39.0	35.0	41.0	32.0	41.0
40	37.13352351853884	39.0	35.0	41.0	32.0	41.0
41	37.06139958483239	39.0	35.0	41.0	32.0	41.0
42	36.98200199987651	39.0	35.0	41.0	32.0	41.0
43	36.923062503026244	39.0	35.0	41.0	32.0	41.0
44	36.846905055618514	39.0	35.0	41.0	31.0	41.0
45	36.7991270196998	39.0	35.0	41.0	31.0	41.0
46	36.741459767699816	39.0	35.0	41.0	31.0	41.0
47	36.64324326485392	39.0	35.0	41.0	31.0	41.0
48	36.618989764736874	39.0	35.0	41.0	31.0	41.0
49	36.618435822932305	39.0	35.0	41.0	31.0	41.0
50	36.512635603576676	39.0	35.0	40.0	31.0	41.0
51	35.54814118541769	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	34.0
10	30.0
11	32.0
12	25.0
13	29.0
14	32.0
15	63.0
16	112.0
17	207.0
18	410.0
19	720.0
20	1296.0
21	2082.0
22	3385.0
23	4847.0
24	7037.0
25	10437.0
26	14394.0
27	17830.0
28	20784.0
29	24778.0
30	30646.0
31	39081.0
32	51242.0
33	71667.0
34	131894.0
35	179385.0
36	165049.0
37	259779.0
38	487381.0
39	725910.0
40	532.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.89495984032972	21.558908623590103	26.218194434017622	12.327937102062556
2	31.270969940105875	23.90727538370576	26.46877869380789	18.352975982380475
3	30.50984412779486	23.842819124007892	28.623021501559876	17.02431524663737
4	27.85292245392221	25.728397935445123	27.3402042255054	19.078475385127263
5	24.77905629105977	29.51239350391069	26.04312750123382	19.665422703795723
6	26.29819837868741	33.266670783101354	25.315096046934464	15.12003479127677
7	80.59675568678789	5.36128599788818	8.974567985362077	5.067390329961855
8	81.57363894455207	4.034668672169954	8.522574572249871	5.869117811028106
9	75.99033200526489	5.761083611451802	11.283132671949621	6.965451711333685
10	40.293824592795026	27.129599727071497	17.749814649384156	14.826761030749323
11	28.201368285121443	25.706808864312684	25.91856833363022	20.17325451693565
12	25.447873276594645	22.695399973080292	31.190655041736647	20.666071708588408
13	22.390620925673627	25.021378066836387	32.570756403758274	20.01724460373171
14	19.303472597649456	28.170228493220545	30.805383408132503	21.720915500997496
15	18.684230516196468	25.706675598441496	35.213018831800255	20.396075053561773
16	20.903684756916387	26.04126177903719	31.35075177499035	21.704301689056074
17	20.79374041318639	25.1414061948196	28.963027160917203	25.101826231076803
18	21.273764081205115	25.05589392747405	31.798791633923983	21.871550357396856
19	21.899980409916935	26.79510239038993	28.606363267661393	22.69855393203174
20	23.963069361776416	26.485925569234066	28.65340612019071	20.897598948798805
21	22.723296962115622	27.415943662297177	29.444472331561933	20.416287044025268
22	21.942625488697054	25.00445330119553	27.94318787067347	25.109733339433948
23	20.96605318463231	25.689661988886513	29.25772242407066	24.086562402410514
24	21.81322432777363	23.905320817595005	30.575855155989924	23.705599698641443
25	20.644127261799472	26.039795854454123	28.055886375741345	25.26019050800506
26	19.73814144750724	26.8551608763386	28.628529824235642	24.778167851918518
27	20.498378820676997	26.20682241300959	29.80442344964038	23.490375316673028
28	17.765406756313137	25.869793024775454	30.826928057307878	25.537872161603524
29	19.105350669150152	23.17564575088433	31.05170316004476	26.667300419920757
30	20.660652229826766	24.055067234853112	31.363234344924944	23.921046190395174
31	20.56558924171275	25.25072863115072	27.477023853258288	26.706658273878247
32	20.627957669428675	25.11955059194479	29.238398872748416	25.01409286587812
33	20.897998746412373	23.870494003257907	30.268321947245376	24.96318530308435
34	19.083450644318276	22.591363749639626	30.24322354150499	28.08196206453711
35	18.34475792032389	22.73902233491579	31.777646782362172	27.138572962398143
36	19.724637172560204	23.64340895875377	30.644575923565803	25.987377945120226
37	19.024547129253236	23.750332609403507	31.895986875977005	25.329133385366255
38	18.947874831363144	24.892554391354775	30.61348055362197	25.546090223660112
39	20.533027947185847	22.75403695640296	31.076446190128642	25.63648890628255
40	20.94113246672018	22.032579951748872	31.611952882518583	25.414334699012365
41	18.614976685135836	23.47229558014854	29.154263686071808	28.758464048643823
42	20.020576250511407	22.174641370435143	29.86159450837998	27.94318787067347
43	20.904662039971768	21.965147420927806	30.106581601580356	27.023608937520073
44	19.671686199741554	23.24032412036751	29.621982471984182	27.46600720790675
45	20.500288964830695	22.307196490310016	29.522699397949214	27.669815146910075
46	21.410716974829185	22.840304397018578	30.472440839948135	25.276537788204106
47	18.22126487968979	22.63911735348195	32.166116796874825	26.973500969953434
48	18.39770889314254	21.111757203797723	32.68287742338434	27.8076564796754
49	20.11017533790672	19.87069656738211	33.70746986303378	26.311658231677388
50	19.306848666386216	20.098403519285128	32.82214025877567	27.77260755555299
51	18.39770889314254	20.295459320814928	30.46426719984861	30.842564586193923
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	386.0
1	1006.0
2	1626.0
3	6193.0
4	10760.0
5	7360.5
6	3961.0
7	3979.0
8	3997.0
9	4090.0
10	4183.0
11	4199.5
12	4216.0
13	4244.0
14	4272.0
15	4210.0
16	4148.0
17	4314.0
18	4480.0
19	4444.5
20	4409.0
21	4948.5
22	5488.0
23	6449.5
24	7411.0
25	9272.0
26	13654.5
27	16176.0
28	19790.5
29	23405.0
30	26887.5
31	30370.0
32	34625.0
33	38880.0
34	48286.5
35	57693.0
36	61965.0
37	66237.0
38	71155.5
39	76074.0
40	87141.5
41	98209.0
42	111726.0
43	125243.0
44	141703.5
45	158164.0
46	188058.0
47	217952.0
48	244682.0
49	271412.0
50	264297.5
51	257183.0
52	220349.5
53	183516.0
54	156482.0
55	129448.0
56	116666.0
57	103884.0
58	93890.0
59	83896.0
60	78212.0
61	72528.0
62	65052.5
63	57577.0
64	49541.0
65	41505.0
66	33583.0
67	25661.0
68	21808.0
69	17955.0
70	15449.0
71	12943.0
72	10113.5
73	7284.0
74	5423.5
75	2674.0
76	1785.0
77	1454.0
78	1123.0
79	788.5
80	454.0
81	442.0
82	430.0
83	250.5
84	71.0
85	49.0
86	27.0
87	19.5
88	12.0
89	7.5
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	1.5
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2251139.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.49564394240463
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.49833712162972	13.424305964645065
2	12.882544882798	5.280721059797492
3	6.0209598436996785	3.702103474439545
4	3.3534106890732502	2.7492124590359643
5	2.1846809922025954	2.2388221871961833
6	1.4540738723156503	1.7881308211761062
7	1.0129350772826418	1.4532529676530004
8	0.7306308082738083	1.1979799119785024
9	0.550532584657829	1.0155167850454736
>10	3.8442206221509485	16.709466398561585
>50	1.6013773253789085	24.278699941965947
>100	0.8545835183708097	22.586047934856037
>500	0.007292789650677039	0.9933894703136412
>1k	0.0035358980124494737	1.2253856798224172
>5k	6.629808773342763E-4	0.8770290130258543
>10k+	2.209936257780921E-4	0.4799359304871121
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	10596	0.4706950570355718	RNA PCR Primer, Index 35 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	7697	0.3419158035110227	RNA PCR Primer, Index 10 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5865	0.26053477817229415	No Hit
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT	5801	0.25769177292028617	TruSeq Adapter, Index 10 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	4089	0.18164138242907257	RNA PCR Primer, Index 35 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	2767	0.12291555519228267	TruSeq Adapter, Index 10 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG	2312	0.1027035647287884	TruSeq Adapter, Index 10 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.221097853131237E-4	0.0	0.0	0.2739057872481442	0.0
2	2.221097853131237E-4	0.0	0.0	1.4583728503659703	0.0
3	2.221097853131237E-4	0.0	0.0	2.1205265423414548	0.0
4	2.221097853131237E-4	0.0	0.0	2.8723237436693156	0.0
5	2.221097853131237E-4	0.0	0.0	4.8096985570415685	0.0
6	2.221097853131237E-4	0.0	0.0	5.815056289282892	0.0
7	2.221097853131237E-4	0.0	0.0	6.9228066325535655	0.0
8	2.221097853131237E-4	0.0	0.0	8.680361363736314	0.0
9	2.221097853131237E-4	0.0	0.0	9.270862438969784	0.0
10	2.221097853131237E-4	0.0	0.0	10.672375184295595	0.0
11	2.6653174237574847E-4	0.0	0.0	12.608905980483657	0.0
12	2.6653174237574847E-4	0.0	0.0	14.190816293440786	0.0
13	2.6653174237574847E-4	0.0	0.0	14.777452658409809	0.0
14	2.6653174237574847E-4	0.0	0.0	14.998762848495806	0.0
15	2.6653174237574847E-4	0.0	0.0	15.421571035817868	0.0
16	2.6653174237574847E-4	0.0	0.0	16.352211036279858	0.0
17	2.6653174237574847E-4	0.0	0.0	17.520153131370385	0.0
18	2.6653174237574847E-4	0.0	0.0	18.80323693916724	0.0
19	2.6653174237574847E-4	0.0	0.0	19.563296624508748	0.0
20	2.6653174237574847E-4	0.0	0.0	20.2672069561231	0.0
21	2.6653174237574847E-4	0.0	0.0	21.155290721719094	0.0
22	2.6653174237574847E-4	0.0	0.0	22.06136537992545	0.0
23	2.6653174237574847E-4	0.0	0.0	22.980899891121783	0.0
24	2.6653174237574847E-4	0.0	0.0	23.740026715364976	0.0
25	2.6653174237574847E-4	0.0	0.0	24.384722578214852	0.0
26	2.6653174237574847E-4	0.0	0.0	24.974957121705945	0.0
27	2.6653174237574847E-4	0.0	0.0	25.526189186896055	0.0
28	2.6653174237574847E-4	0.0	0.0	26.087904833952944	0.0
29	2.6653174237574847E-4	0.0	0.0	26.68609090775825	0.0
30	2.6653174237574847E-4	0.0	0.0	27.36881196585373	0.0
31	3.5537565650099794E-4	0.0	0.0	27.996094421535055	0.0
32	3.5537565650099794E-4	0.0	0.0	28.581531393663386	0.0
33	3.5537565650099794E-4	0.0	0.0	29.16790122689003	0.0
34	3.5537565650099794E-4	0.0	0.0	29.73992276798545	0.0
35	3.5537565650099794E-4	0.0	0.0	30.354500543946862	0.0
36	3.5537565650099794E-4	0.0	0.0	30.92918740246604	0.0
37	3.5537565650099794E-4	0.0	0.0	31.504673856212342	0.0
38	3.5537565650099794E-4	0.0	0.0	32.07522947272469	0.0
39	3.997976135636227E-4	0.0	0.0	32.63943274937709	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCGT	30	2.1664328E-6	45.000004	36
ACGACCG	30	2.1664328E-6	45.000004	1
ACGCGAA	25	3.8920025E-5	45.0	13
GGTCGAT	25	3.8920025E-5	45.0	42
GGCCGTA	20	7.034729E-4	45.0	10
CTGCGTA	25	3.8920025E-5	45.0	1
CCCGTTG	25	3.8920025E-5	45.0	19
TCGGTCG	20	7.034729E-4	45.0	14
TACGTAG	85	0.0	45.0	1
TATCCGT	35	1.2126293E-7	45.0	18
CGTAAGG	300	0.0	43.5	2
GATTACG	100	0.0	40.5	1
ATCGGGT	95	0.0	40.26316	4
TTAAGCG	90	0.0	40.0	1
CGTTTTT	3965	0.0	39.779324	1
ACGCATG	115	0.0	39.130432	1
TCGGTAC	70	0.0	38.571426	36
TACGGGA	740	0.0	38.006756	4
CATATCG	60	1.5643309E-10	37.500004	2
GTATACG	60	1.5643309E-10	37.500004	1
>>END_MODULE