##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934744.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	842160
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.154664196827206	33.0	31.0	34.0	30.0	34.0
2	32.351730075045126	34.0	31.0	34.0	30.0	34.0
3	32.323385104968175	34.0	31.0	34.0	30.0	34.0
4	35.90653438776479	37.0	35.0	37.0	35.0	37.0
5	35.800843070200436	37.0	35.0	37.0	35.0	37.0
6	35.731229220100694	37.0	35.0	37.0	33.0	37.0
7	36.051084117032396	37.0	35.0	37.0	35.0	37.0
8	35.89912486938349	37.0	35.0	37.0	35.0	37.0
9	37.605975111617745	39.0	38.0	39.0	35.0	39.0
10	37.3655504892182	39.0	37.0	39.0	34.0	39.0
11	37.182198157119785	39.0	37.0	39.0	34.0	39.0
12	36.96605158164719	39.0	35.0	39.0	33.0	39.0
13	36.806526075805074	39.0	35.0	39.0	33.0	39.0
14	37.90189631423957	40.0	36.0	41.0	33.0	41.0
15	38.02564120832146	40.0	36.0	41.0	33.0	41.0
16	38.1322670276432	40.0	36.0	41.0	34.0	41.0
17	38.09977795193313	40.0	36.0	41.0	34.0	41.0
18	38.01770328678636	39.0	36.0	41.0	34.0	41.0
19	37.93377624204427	39.0	36.0	41.0	34.0	41.0
20	37.77261090529116	39.0	35.0	41.0	34.0	41.0
21	37.68309228650138	39.0	35.0	41.0	34.0	41.0
22	37.63399472784269	39.0	35.0	41.0	33.0	41.0
23	37.46418257813242	39.0	35.0	41.0	33.0	41.0
24	37.17704236724613	39.0	35.0	41.0	33.0	41.0
25	37.09873420727653	39.0	35.0	41.0	33.0	41.0
26	37.28156644818087	39.0	35.0	41.0	33.0	41.0
27	37.343130759000665	39.0	35.0	41.0	33.0	41.0
28	37.29121425857319	39.0	35.0	41.0	33.0	41.0
29	37.31577847439917	39.0	35.0	41.0	33.0	41.0
30	37.14818205566638	39.0	35.0	41.0	33.0	41.0
31	36.96272917260378	39.0	35.0	41.0	32.0	41.0
32	36.76553861499003	39.0	35.0	41.0	32.0	41.0
33	36.501059181153224	39.0	35.0	41.0	31.0	41.0
34	36.28358031727938	39.0	35.0	41.0	30.0	41.0
35	36.16360786548874	39.0	35.0	41.0	30.0	41.0
36	36.063889284696494	39.0	35.0	41.0	30.0	41.0
37	35.93631851429657	39.0	35.0	41.0	30.0	41.0
38	35.83492091764035	39.0	35.0	41.0	29.0	41.0
39	35.8696245369051	39.0	35.0	41.0	29.0	41.0
40	35.759310582312146	39.0	35.0	41.0	29.0	41.0
41	35.70595730027548	38.0	35.0	41.0	29.0	41.0
42	35.56415764225326	38.0	35.0	40.0	28.0	41.0
43	35.454880307779995	38.0	35.0	40.0	27.0	41.0
44	35.37137954782939	38.0	35.0	40.0	27.0	41.0
45	35.34816068205566	38.0	35.0	40.0	27.0	41.0
46	35.30392324498908	38.0	35.0	40.0	27.0	41.0
47	35.187827728697634	38.0	35.0	40.0	26.0	41.0
48	35.16116889902156	38.0	35.0	40.0	26.0	41.0
49	35.154569203001806	38.0	35.0	40.0	26.0	41.0
50	35.02844827586207	38.0	34.0	40.0	26.0	41.0
51	34.17759214401064	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	17.0
10	15.0
11	16.0
12	11.0
13	11.0
14	16.0
15	24.0
16	65.0
17	112.0
18	235.0
19	438.0
20	750.0
21	1218.0
22	1870.0
23	2965.0
24	4778.0
25	7674.0
26	11321.0
27	14026.0
28	14075.0
29	13703.0
30	14827.0
31	17396.0
32	21746.0
33	29865.0
34	59491.0
35	83355.0
36	62418.0
37	91644.0
38	164062.0
39	223877.0
40	132.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.123824451410655	21.207015294005892	24.953809252398592	16.715351002184857
2	34.36829106108103	23.515958962667426	24.700056996295242	17.4156929799563
3	29.31580697254678	23.600147240429372	30.68823026503277	16.395815521991068
4	27.4508406953548	24.26059181153225	30.5601073430227	17.728460150090246
5	24.500094993825403	28.045145815521995	29.356654317469367	18.098104873183242
6	25.18713783604066	31.199178303410278	28.668899021563597	14.944784838985466
7	75.49610525315855	5.809466134701244	13.545525790823595	5.148902821316614
8	76.27422342547735	4.025244609100408	13.60632183908046	6.094210126341788
9	71.22482663626865	6.133988790728603	16.14847534910231	6.492709223900446
10	42.30918115322504	22.850289731167475	21.02961432506887	13.810914790538614
11	29.95642158259713	22.24850384724993	28.894271872328297	18.90080269782464
12	26.410658307210035	20.39481808682436	34.04115607485513	19.153367531110476
13	21.801201671891327	25.217773344732592	34.774152180108295	18.20687280326779
14	17.431960672556286	28.609171653842502	33.83870048446851	20.120167189132708
15	17.424836135651184	23.162106963047403	40.593236439631426	18.81982046166999
16	18.923363731357462	23.667949083309587	36.59102783319084	20.81765935214211
17	18.660824546404484	22.989693169943955	32.64961527500713	25.69986700864444
18	19.7642965707229	22.45642158259713	35.76256293340933	22.016718913270637
19	21.394628099173556	24.811437256578323	31.92730597511162	21.866628669136507
20	23.75700579462335	24.694594851334664	31.382753870998386	20.1656454830436
21	20.52970931889427	26.00800322979006	33.249501282416645	20.212786168899022
22	20.466538425002376	22.53799753016054	31.561342262752923	25.434121782084162
23	18.957561508501946	24.82164909280897	32.673007504512206	23.54778189417688
24	20.936045407048542	22.24862258953168	34.33041227320224	22.484919730217538
25	18.123634463759856	25.095468794528358	32.68191317564359	24.098983566068206
26	17.887456065355753	25.451220670656404	32.67514486558374	23.986178398404103
27	18.905196162249453	25.695829771064883	31.623563218390803	23.77541084829486
28	15.839982901111426	25.82419017763845	33.81887052341598	24.516956397834143
29	17.37460815047022	22.462239954402964	33.25496342737722	26.908188467749593
30	19.146599221050632	23.45480668756531	32.682150660207085	24.716443431176973
31	21.12662676926	25.80519141255818	29.45236059656122	23.615821221620596
32	21.450911940723852	24.061461005034673	29.570746651467655	24.91688040277382
33	21.62902536335138	23.598128621639596	30.080032297900637	24.69281371710839
34	18.531395459295148	23.569392989455686	32.86845730027548	25.030754250973686
35	17.116699914505556	22.83022228555144	32.317018143820654	27.736059656122354
36	21.47323548969317	24.366628669136507	30.99126056806308	23.168875273107247
37	20.244727842690224	25.545264557803744	32.10933789303695	22.100669706469077
38	19.350479718818278	26.51823881447706	29.75135366201197	24.379927804692695
39	21.901895126816758	23.568086824356417	31.429300845445045	23.10071720338178
40	21.012277951933125	21.87410943288686	33.41027833190843	23.703334283271587
41	18.58934169278997	22.974256673316233	30.64085209461385	27.795549539279946
42	21.867341122827018	21.9718343307685	29.48964567303125	26.67117887337323
43	22.33031727937684	22.20112567683101	29.262610430322027	26.205946613470125
44	19.358910420822646	23.977747696399735	29.50603210791299	27.157309774864636
45	20.029923055001426	22.876294290871094	29.479315094518853	27.614467559608624
46	21.183741806782557	23.178612140210888	31.14883157594756	24.48881447705899
47	17.963094898831574	23.107366771159874	33.983091099078564	24.946447230929987
48	20.2076802507837	20.53255913365631	33.10475444096134	26.15500617459865
49	21.298684335518193	19.50567588106773	34.431343212691175	24.7642965707229
50	19.14434311769735	21.558254963427377	33.440320129191605	25.857081789683672
51	18.318846774959628	21.149781514201578	30.82656502327349	29.70480668756531
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	230.0
1	473.0
2	716.0
3	8838.0
4	16960.0
5	11102.0
6	5244.0
7	5214.5
8	5185.0
9	5175.5
10	5166.0
11	5140.0
12	5114.0
13	4889.5
14	4665.0
15	4386.5
16	4108.0
17	3855.5
18	3603.0
19	3349.0
20	3095.0
21	3029.0
22	2963.0
23	3227.0
24	3491.0
25	3894.0
26	4735.5
27	5174.0
28	6238.5
29	7303.0
30	8798.0
31	10293.0
32	11424.5
33	12556.0
34	14888.5
35	17221.0
36	18622.5
37	20024.0
38	22142.0
39	24260.0
40	28652.5
41	33045.0
42	38915.5
43	44786.0
44	52873.5
45	60961.0
46	69961.5
47	78962.0
48	87429.5
49	95897.0
50	94144.0
51	92391.0
52	78607.5
53	64824.0
54	56893.5
55	48963.0
56	43824.5
57	38686.0
58	34687.5
59	30689.0
60	28935.5
61	27182.0
62	24079.0
63	20976.0
64	17934.5
65	14893.0
66	12705.5
67	10518.0
68	8696.5
69	6875.0
70	5747.0
71	4619.0
72	3609.5
73	2600.0
74	2225.5
75	1255.0
76	659.0
77	633.0
78	607.0
79	431.5
80	256.0
81	188.0
82	120.0
83	95.0
84	70.0
85	40.0
86	10.0
87	28.0
88	46.0
89	25.0
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	842160.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.299257870558932
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.10917952388392	18.701186492624924
2	11.480174701967087	6.038401497721989
3	4.957537154894057	3.911386441183076
4	2.794305139422952	2.939526057228494
5	1.6683889751365693	2.1938695942757085
6	1.1057340791628734	1.744799141050166
7	0.7762049836935405	1.4289530518598592
8	0.5609000578786737	1.1801004209410135
9	0.455177423761889	1.077374559993354
>10	3.3590112668743064	20.307752432724975
>50	1.4451729626923229	27.460969944142978
>100	0.2822852836409782	9.730937324429075
>500	0.0031922406873777826	0.5957104822820846
>1k	0.002280171919555559	1.506246818397564
>5k	4.560343839111118E-4	1.182785741144739
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9862	1.1710363826351287	No Hit
GCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	4382	0.5203286786358886	TruSeq Adapter, Index 27 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	3119	0.3703571767835091	TruSeq Adapter, Index 27 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGCT	2138	0.25387099838510496	TruSeq Adapter, Index 20 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	1714	0.20352427092239006	TruSeq Adapter, Index 27 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	1206	0.14320319179253346	TruSeq Adapter, Index 20 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTG	968	0.11494252873563218	No Hit
ACCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTG	849	0.10081219720718153	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2693074950128242	0.0
2	0.0	0.0	0.0	1.5765412748171368	0.0
3	0.0	0.0	0.0	2.2591906526075807	0.0
4	0.0	0.0	0.0	3.0067920585161962	0.0
5	0.0	0.0	0.0	4.962596181248219	0.0
6	0.0	0.0	0.0	6.008834425762325	0.0
7	0.0	0.0	0.0	7.16764035337703	0.0
8	0.0	0.0	0.0	9.033437826541276	0.0
9	0.0	0.0	0.0	9.694832335898166	0.0
10	0.0	0.0	0.0	11.093497672651278	0.0
11	0.0	0.0	0.0	13.089080459770114	0.0
12	0.0	0.0	0.0	14.673577467464614	0.0
13	0.0	0.0	0.0	15.308492447990881	0.0
14	0.0	0.0	0.0	15.53374655647383	0.0
15	0.0	0.0	0.0	15.967868338557993	0.0
16	0.0	0.0	0.0	16.945592286501377	0.0
17	0.0	0.0	0.0	18.118647287926287	0.0
18	0.0	0.0	0.0	19.513750356226844	0.0
19	0.0	0.0	0.0	20.31953548019379	0.0
20	0.0	0.0	0.0	21.0000474969127	0.0
21	1.1874228175168615E-4	0.0	0.0	21.852617079889807	0.0
22	1.1874228175168615E-4	0.0	0.0	22.644153130046547	0.0
23	1.1874228175168615E-4	0.0	0.0	23.526645768025077	0.0
24	1.1874228175168615E-4	0.0	0.0	24.255960862543933	0.0
25	1.1874228175168615E-4	0.0	0.0	24.85430322029068	0.0
26	1.1874228175168615E-4	0.0	0.0	25.411442006269592	0.0
27	1.1874228175168615E-4	0.0	0.0	25.946850954687946	0.0
28	1.1874228175168615E-4	0.0	0.0	26.495440296380735	0.0
29	1.1874228175168615E-4	0.0	0.0	27.044504607200533	0.0
30	1.1874228175168615E-4	0.0	0.0	27.71884202526836	0.0
31	2.374845635033723E-4	0.0	0.0	28.353757005794623	0.0
32	2.374845635033723E-4	0.0	0.0	28.873491973021753	0.0
33	2.374845635033723E-4	0.0	0.0	29.407713498622588	0.0
34	2.374845635033723E-4	0.0	0.0	29.915811722238054	0.0
35	2.374845635033723E-4	0.0	0.0	30.522941008834426	0.0
36	2.374845635033723E-4	0.0	0.0	31.038876223045502	0.0
37	2.374845635033723E-4	0.0	0.0	31.530706754060986	0.0
38	2.374845635033723E-4	0.0	0.0	32.05032297900637	0.0
39	2.374845635033723E-4	0.0	0.0	32.59463759855609	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACTT	25	3.889914E-5	45.000004	9
GTACAAC	25	3.889914E-5	45.000004	10
ATCGTCC	25	3.889914E-5	45.000004	30
TGCGACG	25	3.889914E-5	45.000004	1
TCGCTAC	25	3.889914E-5	45.000004	25
AGCGCGT	25	3.889914E-5	45.000004	11
CAGCTCG	25	3.889914E-5	45.000004	27
TAGATCC	25	3.889914E-5	45.000004	17
ACGTAAC	50	2.1827873E-11	45.000004	11
CGCGTAA	25	3.889914E-5	45.000004	31
CGTCACA	25	3.889914E-5	45.000004	29
TACGTCA	25	3.889914E-5	45.000004	18
ATAGCGA	20	7.032213E-4	45.0	12
CGCGCAT	20	7.032213E-4	45.0	24
CGTCTAG	20	7.032213E-4	45.0	17
TAACGAG	35	1.2114106E-7	45.0	1
ACTTACG	20	7.032213E-4	45.0	1
GACCCGT	20	7.032213E-4	45.0	10
CGTTAAG	20	7.032213E-4	45.0	41
CTAACGG	35	1.2114106E-7	45.0	2
>>END_MODULE