##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934743.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	851694
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05599311489807	33.0	31.0	34.0	30.0	34.0
2	32.238882744272004	34.0	31.0	34.0	30.0	34.0
3	32.26848844772888	34.0	31.0	34.0	30.0	34.0
4	35.86914783948225	37.0	35.0	37.0	35.0	37.0
5	35.73842600746278	37.0	35.0	37.0	35.0	37.0
6	35.614249953621844	37.0	35.0	37.0	33.0	37.0
7	35.922274901549144	37.0	35.0	37.0	35.0	37.0
8	35.46422424016137	37.0	35.0	37.0	33.0	37.0
9	37.35690165716795	39.0	37.0	39.0	34.0	39.0
10	37.24534163678504	39.0	37.0	39.0	34.0	39.0
11	37.15200294941611	39.0	37.0	39.0	34.0	39.0
12	36.89107120632528	39.0	35.0	39.0	33.0	39.0
13	36.62895828783577	39.0	35.0	39.0	33.0	39.0
14	37.68524728364882	40.0	36.0	41.0	33.0	41.0
15	37.686393235129046	40.0	36.0	41.0	33.0	41.0
16	37.9759866806623	40.0	36.0	41.0	34.0	41.0
17	37.841110774527	40.0	36.0	41.0	33.0	41.0
18	37.8782320880504	39.0	36.0	41.0	33.0	41.0
19	37.83493132510033	39.0	36.0	41.0	34.0	41.0
20	37.620157004745835	39.0	35.0	41.0	33.0	41.0
21	37.67986389477911	39.0	35.0	41.0	33.0	41.0
22	37.60329296672279	39.0	35.0	41.0	33.0	41.0
23	37.362949603965745	39.0	35.0	41.0	33.0	41.0
24	37.120455233922044	39.0	35.0	41.0	32.0	41.0
25	37.074721672337716	39.0	35.0	40.0	32.0	41.0
26	37.297438986302595	39.0	35.0	41.0	33.0	41.0
27	37.320281697417144	39.0	35.0	41.0	33.0	41.0
28	37.299222490706754	39.0	35.0	41.0	33.0	41.0
29	37.33328989050058	39.0	35.0	41.0	33.0	41.0
30	37.143172313060795	39.0	35.0	41.0	32.0	41.0
31	37.03891655923371	39.0	35.0	41.0	32.0	41.0
32	36.83174238635003	39.0	35.0	41.0	32.0	41.0
33	36.501440658264585	39.0	35.0	40.0	31.0	41.0
34	36.48378290794581	39.0	35.0	40.0	31.0	41.0
35	36.459748454257046	39.0	35.0	41.0	31.0	41.0
36	36.27969435031831	39.0	35.0	40.0	30.0	41.0
37	36.17851247044126	39.0	35.0	40.0	30.0	41.0
38	35.898220487639925	38.0	35.0	40.0	30.0	41.0
39	36.02258909890172	38.0	35.0	40.0	30.0	41.0
40	35.83021836481178	38.0	35.0	40.0	30.0	41.0
41	35.81003975606262	38.0	35.0	40.0	29.0	41.0
42	35.846084391812084	38.0	35.0	40.0	30.0	41.0
43	35.733123633605494	38.0	35.0	40.0	29.0	41.0
44	35.72906936059195	38.0	35.0	40.0	29.0	41.0
45	35.710924346067955	38.0	35.0	40.0	29.0	41.0
46	35.631515544315214	38.0	35.0	40.0	29.0	41.0
47	35.309660511873986	38.0	34.0	40.0	28.0	41.0
48	35.33224256599201	38.0	35.0	40.0	28.0	41.0
49	35.42206355803845	38.0	35.0	40.0	28.0	41.0
50	35.274442464077474	38.0	34.0	40.0	27.0	41.0
51	34.209793658285726	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	6.0
10	8.0
11	8.0
12	9.0
13	7.0
14	10.0
15	19.0
16	51.0
17	84.0
18	220.0
19	458.0
20	945.0
21	1705.0
22	2759.0
23	3877.0
24	5130.0
25	6609.0
26	8578.0
27	9522.0
28	10394.0
29	11702.0
30	14351.0
31	18717.0
32	24675.0
33	34132.0
34	60911.0
35	91902.0
36	68487.0
37	100017.0
38	172743.0
39	203500.0
40	154.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.436043931271094	19.192573858686334	21.996515180334722	12.374867029707854
2	27.144843100925918	34.1072028216707	22.051229666993073	16.69672441041031
3	25.465484082311253	33.165080416205825	26.00499709989738	15.364438401585545
4	24.617644365229765	22.373998173052765	37.428818331466466	15.579539130251005
5	30.608411002073517	24.596744840283012	25.635498195361244	19.159345962282227
6	21.69675963432876	37.975493545804014	25.795062545937856	14.53268427392937
7	68.34309035874386	5.540487545996567	20.374453735731375	5.741968359528187
8	65.03392063346695	13.42583134318194	13.087916552188933	8.452331471162179
9	60.97988244604283	5.549998003977954	14.85932741101851	18.610792138960708
10	41.57619990278199	20.864301028303593	21.544005241319063	16.015493827595357
11	33.156157023531925	20.697926720160055	25.974821943092234	20.17109431321578
12	27.436379732626975	18.479054683959262	31.258527123591335	22.826038459822424
13	22.382451913480665	26.02108268932269	35.57251782917339	16.02394756802326
14	17.229192644306522	34.13949141358281	26.479463281413278	22.151852660697386
15	12.481595502610093	23.27925287720707	44.44366169070112	19.795489929481715
16	13.795212834656578	24.15996825150817	32.51531653387249	29.529502379962757
17	13.393073099023828	25.693969899987557	32.50909364161307	28.403863359375553
18	16.08911181715499	24.142591118406376	34.660570580513664	25.10772648392498
19	17.23612001493494	27.399159792131915	29.14626614723128	26.21845404570186
20	20.317273574781552	26.08061111150249	34.13726056541434	19.46485474830162
21	18.141844371335246	32.0571707679049	29.76538522051347	20.035599640246378
22	15.875185219104516	24.48191486613737	30.206623505625256	29.436276409132855
23	15.828807059812561	30.139345821386556	28.434155929242195	25.59769118955869
24	20.325727315209452	23.103015871897654	29.24054883561467	27.330707977278223
25	14.162833130208737	33.29129945731683	27.779812937510425	24.766054474964015
26	14.899600091112536	25.918933325818898	32.35446063961939	26.827005943449173
27	21.752765664663603	28.283514971339475	27.43356181915101	22.53015754484592
28	13.746721240257651	27.908615065974402	34.88623848471399	23.458425209053956
29	19.994152829537367	20.5165235401447	34.230016883998246	25.259306746319687
30	22.07130730050934	27.112084856767805	31.808489903650845	19.008117939072015
31	23.59438953426935	28.01334751683116	23.049592928915786	25.342670019983704
32	29.127949709637495	28.187118847849106	23.996294443779103	18.68863699873429
33	21.71437159355355	25.854003902810163	25.74434010337046	26.68728440026582
34	21.121200806862557	24.501522847407635	28.929404222643345	25.447872123086462
35	25.654871350508518	21.766972645104932	26.920701566525068	25.65745443786149
36	21.598132662669926	29.323325043971195	29.036484934730076	20.042057358628803
37	25.96507665898785	24.390097852045454	29.85414949500642	19.79067599396027
38	21.082571909629515	28.970029141921867	26.451636385838107	23.495762562610516
39	24.827813745312284	23.827924113590093	28.510122180031793	22.834139961065826
40	23.42355352978887	20.633701775520315	32.84783032403657	23.094914370654248
41	19.16615591984915	26.222328676731316	24.60120653661996	30.01030886679958
42	25.166080775489792	22.430473855633597	30.207680223178745	22.19576514569787
43	24.816072439162422	26.040338431408465	26.45950306095851	22.684086068470602
44	22.51982519543404	23.47239736337229	29.297024518195503	24.710752922998168
45	19.910554729750356	22.332433949282255	27.52079972384448	30.23621159712291
46	28.390947922610703	24.400782440641827	26.66626746225757	20.542002174489898
47	18.274168891644184	22.317287664348935	37.154893658990204	22.253649785016684
48	21.757579600185043	23.042665558287368	27.692222793632453	27.507532047895133
49	21.58509981284358	17.98791584771056	36.16686274647937	24.260121592966488
50	21.987122135414833	19.478709489558458	31.70082212625661	26.833346248770095
51	20.268781980382624	18.828710781102135	27.378260267185162	33.52424697133008
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	333.0
1	545.5
2	758.0
3	3557.5
4	6357.0
5	4316.5
6	2276.0
7	2330.5
8	2385.0
9	2501.0
10	2617.0
11	2688.5
12	2760.0
13	2790.0
14	2820.0
15	2616.5
16	2413.0
17	2363.0
18	2313.0
19	2245.5
20	2178.0
21	2179.0
22	2180.0
23	2273.5
24	2367.0
25	2814.0
26	4388.5
27	5516.0
28	5976.0
29	6436.0
30	8492.0
31	10548.0
32	10749.0
33	10950.0
34	13960.0
35	16970.0
36	17433.0
37	17896.0
38	20787.0
39	23678.0
40	29532.5
41	35387.0
42	40951.0
43	46515.0
44	54304.5
45	62094.0
46	113016.5
47	163939.0
48	139531.0
49	115123.0
50	108969.0
51	102815.0
52	80798.0
53	58781.0
54	48337.5
55	37894.0
56	32230.5
57	26567.0
58	23754.0
59	20941.0
60	18438.0
61	15935.0
62	14852.5
63	13770.0
64	10899.0
65	8028.0
66	7111.0
67	6194.0
68	4985.5
69	3777.0
70	3356.0
71	2935.0
72	2355.5
73	1776.0
74	1309.5
75	854.0
76	865.0
77	602.5
78	340.0
79	229.0
80	118.0
81	73.5
82	29.0
83	20.0
84	11.0
85	6.5
86	2.0
87	1.5
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	851694.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.777489753398516
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.51638297650618	15.378537214338994
2	12.296666355015278	5.601743838046415
3	5.779122234682158	3.9490169245233115
4	3.274067629046796	2.983001674901887
5	2.076108741260917	2.3644272790505814
6	1.4335820416564395	1.9592040158691408
7	1.0128417952675783	1.6148995529464714
8	0.7469460251475479	1.3610844347312019
9	0.5822179316786348	1.1935316675749486
>10	3.749813259475991	17.635157440081056
>50	1.1057588757022745	18.179826639583734
>100	0.4093284267059531	12.850282460847492
>500	0.006761460669856913	1.1682182663078926
>1k	0.007281573029076675	3.919176218030393
>5k	0.0015603370776592876	2.4098870269987986
>10k+	0.0015603370776592876	7.432005346167664
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGC	24305	2.8537244597237974	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCG	20915	2.4556941812434983	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTC	17514	2.0563723590867142	No Hit
GCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC	8804	1.0337045934337918	No Hit
CCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC	6253	0.7341838735508293	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGAGTCGAT	5285	0.6205280300201715	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCT	4534	0.532350820834713	No Hit
CTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGCT	4299	0.5047587513825388	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4018	0.4717656811014284	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGAGTCGATCGTA	3379	0.39673873480381455	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTC	2913	0.34202424814546073	No Hit
GAACTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCT	2882	0.3383844432390037	No Hit
TCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC	2085	0.24480623322460882	No Hit
CGCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTG	1814	0.2129872935584846	No Hit
ACTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC	1778	0.20876042334453454	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATG	1155	0.13561208603089842	No Hit
GCCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTG	1133	0.13302899867792892	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGAGTCGA	1045	0.12269664926605095	No Hit
ACCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTG	1037	0.12175734477406204	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1010	0.11858719211359947	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGC	972	0.11412549577665218	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGAGTCGATCGT	936	0.1098986255627021	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCC	915	0.10743295127123122	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTAT	905	0.10625882065624509	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTC	900	0.105671755348752	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1741306149861335E-4	0.0	0.0	0.5419786918775993	0.0
2	1.1741306149861335E-4	0.0	0.0	2.923467818253974	0.0
3	1.1741306149861335E-4	0.0	0.0	3.890364379695055	0.0
4	1.1741306149861335E-4	0.0	0.0	5.1880135353777295	0.0
5	1.1741306149861335E-4	0.0	0.0	9.784265240802448	0.0
6	1.1741306149861335E-4	0.0	0.0	11.133458730483014	0.0
7	1.1741306149861335E-4	0.0	0.0	13.13805192944884	0.0
8	1.1741306149861335E-4	0.0	0.0	15.79334831523998	0.0
9	1.1741306149861335E-4	0.0	0.0	16.634378074754547	0.0
10	1.1741306149861335E-4	0.0	0.0	21.039363902998026	0.0
11	1.1741306149861335E-4	0.0	0.0	23.582061162811996	0.0
12	1.1741306149861335E-4	0.0	0.0	28.61755513130303	0.0
13	1.1741306149861335E-4	0.0	0.0	29.51329937747595	0.0
14	1.1741306149861335E-4	0.0	0.0	29.907572437988293	0.0
15	1.1741306149861335E-4	0.0	0.0	30.860849084295534	0.0
16	1.1741306149861335E-4	0.0	0.0	32.08206233694261	0.0
17	1.1741306149861335E-4	0.0	0.0	33.5563007371192	0.0
18	1.1741306149861335E-4	0.0	0.0	35.02208539686789	0.0
19	1.1741306149861335E-4	0.0	0.0	36.579334831524	0.0
20	1.1741306149861335E-4	0.0	0.0	37.44466909476878	0.0
21	2.348261229972267E-4	0.0	0.0	38.36002132221197	0.0
22	2.348261229972267E-4	0.0	0.0	39.37188708620702	0.0
23	2.348261229972267E-4	0.0	0.0	40.32316771046878	0.0
24	2.348261229972267E-4	0.0	0.0	41.10419939555756	0.0
25	2.348261229972267E-4	0.0	0.0	41.74527471133999	0.0
26	2.348261229972267E-4	0.0	0.0	42.312614624501286	0.0
27	2.348261229972267E-4	0.0	0.0	42.948054113331786	0.0
28	2.348261229972267E-4	0.0	0.0	43.462792974941706	0.0
29	2.348261229972267E-4	0.0	0.0	44.07944637393242	0.0
30	2.348261229972267E-4	0.0	0.0	44.7582112824559	0.0
31	2.348261229972267E-4	0.0	0.0	45.3680547238797	0.0
32	2.348261229972267E-4	0.0	0.0	45.89852693573044	0.0
33	2.348261229972267E-4	0.0	0.0	46.43076034350365	0.0
34	2.348261229972267E-4	0.0	0.0	46.985184819900105	0.0
35	2.348261229972267E-4	0.0	0.0	47.59808100092287	0.0
36	2.348261229972267E-4	0.0	0.0	48.12233032051417	0.0
37	2.348261229972267E-4	0.0	0.0	48.63530798620162	0.0
38	2.348261229972267E-4	0.0	0.0	49.109656754656015	0.0
39	2.348261229972267E-4	0.0	0.0	49.5769607394205	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGAAT	30	2.1648375E-6	45.000004	41
AGATCGA	30	2.1648375E-6	45.000004	13
TACCAGT	30	2.1648375E-6	45.000004	25
GAGTATA	30	2.1648375E-6	45.000004	13
CGACCGA	30	2.1648375E-6	45.000004	25
AGTATAT	30	2.1648375E-6	45.000004	14
GTTCGTT	30	2.1648375E-6	45.000004	28
ACTCGTT	30	2.1648375E-6	45.000004	24
GACGGAC	30	2.1648375E-6	45.000004	4
ACCGTCT	30	2.1648375E-6	45.000004	38
GACGCAC	30	2.1648375E-6	45.000004	14
CGTTTAA	30	2.1648375E-6	45.000004	32
AATCGTT	30	2.1648375E-6	45.000004	22
GACTAGA	30	2.1648375E-6	45.000004	30
GTGCCAA	30	2.1648375E-6	45.000004	1
ACGCGAT	25	3.8899514E-5	45.0	44
TCCGGAT	25	3.8899514E-5	45.0	45
CGGGTTC	20	7.032256E-4	45.0	6
AACGTGC	20	7.032256E-4	45.0	22
GTGCAAG	70	0.0	45.0	1
>>END_MODULE