##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934742.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	491520
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12763468424479	33.0	31.0	34.0	30.0	34.0
2	32.293922932942706	34.0	31.0	34.0	30.0	34.0
3	32.28111572265625	34.0	31.0	34.0	30.0	34.0
4	35.88071085611979	37.0	35.0	37.0	35.0	37.0
5	35.750482177734376	37.0	35.0	37.0	35.0	37.0
6	35.70614827473958	37.0	35.0	37.0	33.0	37.0
7	36.06855875651042	37.0	35.0	37.0	35.0	37.0
8	35.719502766927086	37.0	35.0	37.0	35.0	37.0
9	37.538429768880206	39.0	38.0	39.0	35.0	39.0
10	37.25702107747396	39.0	37.0	39.0	34.0	39.0
11	37.17726236979167	39.0	37.0	39.0	34.0	39.0
12	37.08776652018229	39.0	37.0	39.0	34.0	39.0
13	37.08224690755208	39.0	37.0	39.0	33.0	39.0
14	38.184086100260416	40.0	37.0	41.0	33.0	41.0
15	38.255000813802084	40.0	37.0	41.0	34.0	41.0
16	38.32835896809896	40.0	37.0	41.0	34.0	41.0
17	38.28602091471354	40.0	37.0	41.0	34.0	41.0
18	38.33519287109375	40.0	37.0	41.0	34.0	41.0
19	38.255141194661455	40.0	37.0	41.0	34.0	41.0
20	38.21196492513021	40.0	37.0	41.0	34.0	41.0
21	38.108046468098955	40.0	36.0	41.0	34.0	41.0
22	38.071608479817705	40.0	36.0	41.0	34.0	41.0
23	37.9253662109375	40.0	36.0	41.0	34.0	41.0
24	37.63364054361979	39.0	36.0	41.0	33.0	41.0
25	37.55296223958333	39.0	35.0	41.0	33.0	41.0
26	37.750362141927084	39.0	36.0	41.0	33.0	41.0
27	37.87926025390625	40.0	36.0	41.0	34.0	41.0
28	37.86243693033854	40.0	36.0	41.0	34.0	41.0
29	37.88491617838542	40.0	36.0	41.0	34.0	41.0
30	37.748313395182294	40.0	36.0	41.0	33.0	41.0
31	37.700968424479164	40.0	36.0	41.0	33.0	41.0
32	37.571248372395836	40.0	36.0	41.0	33.0	41.0
33	37.42296549479167	40.0	36.0	41.0	33.0	41.0
34	37.36676839192708	40.0	36.0	41.0	33.0	41.0
35	37.31851603190104	40.0	36.0	41.0	33.0	41.0
36	37.249273681640624	40.0	35.0	41.0	33.0	41.0
37	37.1560546875	39.0	35.0	41.0	32.0	41.0
38	37.0800537109375	39.0	35.0	41.0	32.0	41.0
39	37.1452392578125	39.0	35.0	41.0	32.0	41.0
40	37.06431477864583	39.0	35.0	41.0	32.0	41.0
41	37.02939046223958	39.0	35.0	41.0	32.0	41.0
42	36.969040934244795	39.0	35.0	41.0	32.0	41.0
43	36.904754638671875	39.0	35.0	41.0	32.0	41.0
44	36.853399658203124	39.0	35.0	41.0	31.0	41.0
45	36.76902669270833	39.0	35.0	41.0	31.0	41.0
46	36.7366943359375	39.0	35.0	41.0	31.0	41.0
47	36.680092366536456	39.0	35.0	41.0	31.0	41.0
48	36.65023396809896	39.0	35.0	41.0	31.0	41.0
49	36.61455078125	39.0	35.0	41.0	31.0	41.0
50	36.53994140625	39.0	35.0	41.0	31.0	41.0
51	35.62240397135417	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	6.0
11	4.0
12	2.0
13	3.0
14	3.0
15	6.0
16	23.0
17	35.0
18	69.0
19	147.0
20	232.0
21	420.0
22	651.0
23	984.0
24	1566.0
25	2410.0
26	3405.0
27	4197.0
28	4752.0
29	5367.0
30	6391.0
31	8407.0
32	11220.0
33	16080.0
34	28473.0
35	42895.0
36	37367.0
37	57057.0
38	104701.0
39	154564.0
40	81.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.869466145833336	22.263997395833332	27.044677734375	12.821858723958332
2	31.339518229166668	23.554280598958332	27.146402994791668	17.959798177083332
3	29.285481770833332	23.306070963541668	30.561930338541664	16.846516927083332
4	27.20458984375	25.237833658854164	29.607747395833332	17.9498291015625
5	24.345296223958332	28.433837890625004	28.546752929687504	18.674112955729168
6	24.969075520833332	32.081298828125	26.720784505208332	16.228841145833332
7	79.12150065104167	4.975992838541667	10.13671875	5.765787760416666
8	79.37540690104167	3.6120605468749996	10.2301025390625	6.782430013020833
9	74.98779296875	5.310465494791667	12.7386474609375	6.963094075520833
10	37.70345052083333	27.031656901041668	20.141194661458332	15.123697916666668
11	26.139933268229164	26.962890625000004	27.843424479166668	19.053751627604168
12	23.475748697916668	23.6810302734375	33.53373209635417	19.309488932291664
13	20.6134033203125	26.121419270833336	34.85616048177083	18.409016927083332
14	17.469482421875	29.651896158854168	32.713623046875	20.164998372395836
15	16.376139322916668	25.870361328125004	38.4649658203125	19.288533528645832
16	17.5140380859375	25.961100260416664	35.1165771484375	21.408284505208332
17	18.248697916666668	25.6243896484375	30.519816080729168	25.607096354166664
18	19.192708333333332	25.611368815104168	34.089762369791664	21.106160481770832
19	19.4390869140625	26.871337890625004	31.6650390625	22.0245361328125
20	20.680745442708336	27.474568684895832	30.946248372395836	20.8984375
21	19.705810546875	27.997233072916668	32.34375	19.953206380208332
22	19.035441080729168	25.743204752604164	29.558919270833332	25.662434895833336
23	17.508341471354168	26.682535807291668	31.027832031250004	24.781290690104164
24	20.349527994791668	24.3017578125	31.662190755208336	23.6865234375
25	16.805216471354168	27.484334309895836	30.737915039062504	24.9725341796875
26	17.374674479166664	28.235066731770836	32.0050048828125	22.38525390625
27	20.81298828125	27.186279296875	30.4058837890625	21.5948486328125
28	16.800130208333332	26.043294270833332	32.8070068359375	24.349568684895832
29	18.810628255208332	24.367472330729168	32.5811767578125	24.24072265625
30	23.46435546875	25.313720703124996	28.789876302083332	22.432047526041668
31	21.158040364583332	26.500244140625	26.5667724609375	25.774943033854164
32	20.914103190104168	27.808227539062504	28.513387044270832	22.7642822265625
33	23.162841796875	25.0811767578125	28.069254557291668	23.686726888020836
34	18.941446940104168	23.34228515625	30.663655598958332	27.0526123046875
35	19.273274739583332	23.997395833333332	31.667073567708332	25.062255859375
36	23.1768798828125	22.688191731770832	31.702473958333332	22.432454427083336
37	20.310262044270832	24.715576171875	31.2481689453125	23.725992838541668
38	19.7711181640625	27.030029296875004	28.812459309895832	24.386393229166668
39	21.744588216145832	23.907470703125	31.163126627604164	23.184814453125
40	21.933797200520832	21.927693684895832	32.152913411458336	23.985595703125
41	18.441365559895832	22.582804361979168	31.780598958333332	27.195231119791668
42	20.932210286458332	21.987101236979168	33.473917643229164	23.606770833333332
43	23.259073893229168	22.050374348958332	31.453043619791664	23.237508138020832
44	21.1041259765625	24.1094970703125	28.10546875	26.680908203124996
45	21.786295572916668	23.164469401041668	27.666015625	27.383219401041664
46	24.019571940104168	22.327880859375	29.9700927734375	23.682454427083332
47	18.601888020833332	22.374267578125	34.140218098958336	24.883626302083332
48	19.765421549479168	20.638631184895832	33.316650390625	26.279296875000004
49	21.737874348958332	19.012044270833332	34.7314453125	24.518636067708332
50	20.400797526041668	20.184326171875	32.4847412109375	26.930135091145836
51	19.9609375	19.798177083333332	29.501139322916664	30.739746093749996
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	176.0
1	221.5
2	267.0
3	1847.5
4	3428.0
5	2276.5
6	1125.0
7	1168.0
8	1211.0
9	1238.0
10	1265.0
11	1362.5
12	1460.0
13	1413.0
14	1366.0
15	1342.5
16	1319.0
17	1272.0
18	1225.0
19	1405.5
20	1586.0
21	1644.0
22	1702.0
23	1911.5
24	2121.0
25	2317.5
26	3344.5
27	4175.0
28	5155.0
29	6135.0
30	6952.5
31	7770.0
32	8745.0
33	9720.0
34	10632.5
35	11545.0
36	12956.0
37	14367.0
38	15744.0
39	17121.0
40	19115.5
41	21110.0
42	25551.5
43	29993.0
44	35183.5
45	40374.0
46	47151.0
47	53928.0
48	60378.0
49	66828.0
50	64725.0
51	62622.0
52	50348.5
53	38075.0
54	31565.0
55	25055.0
56	21199.5
57	17344.0
58	15306.5
59	13269.0
60	11937.0
61	10605.0
62	9028.0
63	7451.0
64	6180.5
65	4910.0
66	4173.0
67	3436.0
68	2691.0
69	1946.0
70	1537.5
71	1129.0
72	895.0
73	661.0
74	630.0
75	451.0
76	303.0
77	215.5
78	128.0
79	116.5
80	105.0
81	75.5
82	46.0
83	25.5
84	5.0
85	2.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	491520.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.49660630216732
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.39940967456822	22.55245293797266
2	12.700691725136625	8.254587575139185
3	5.1688353457730125	5.0390882181693755
4	2.729940374277723	3.5485519028517793
5	1.5151153419340404	2.461805338460207
6	1.0719195712710499	2.090024897710998
7	0.7192159528673163	1.6360454366597117
8	0.5500331095238035	1.4299367530681542
9	0.483411784392126	1.4138318195297201
>10	5.373813898567047	41.05383518947963
>50	0.2300905773512442	4.59500106303484
>100	0.051895429130851274	2.994116102802627
>500	0.0025009845364265676	0.6341365605895086
>1k	0.003126230670533209	2.2965862045316294
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC	2851	0.5800374348958334	TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC	2821	0.5739339192708334	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2141	0.43558756510416663	No Hit
CTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGCT	2082	0.42358398437500006	TruSeq Adapter, Index 19 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC	1408	0.2864583333333333	TruSeq Adapter, Index 13 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTG	924	0.18798828125	No Hit
ACTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC	920	0.18717447916666669	TruSeq Adapter, Index 19 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTG	661	0.13448079427083334	No Hit
GCCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTG	616	0.12532552083333334	No Hit
AGCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTG	493	0.10030110677083333	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4429117838541667	0.0
2	0.0	0.0	0.0	2.1331787109375	0.0
3	0.0	0.0	0.0	3.0985514322916665	0.0
4	0.0	0.0	0.0	4.08203125	0.0
5	0.0	0.0	0.0	6.592000325520833	0.0
6	0.0	0.0	0.0	7.9766845703125	0.0
7	0.0	0.0	0.0	9.482421875	0.0
8	0.0	0.0	0.0	11.888427734375	0.0
9	0.0	0.0	0.0	12.79296875	0.0
10	0.0	0.0	0.0	14.766845703125	0.0
11	0.0	0.0	0.0	17.5018310546875	0.0
12	0.0	0.0	0.0	19.516398111979168	0.0
13	0.0	0.0	0.0	20.306396484375	0.0
14	0.0	0.0	0.0	20.583089192708332	0.0
15	0.0	0.0	0.0	21.139119466145832	0.0
16	0.0	0.0	0.0	22.350667317708332	0.0
17	0.0	0.0	0.0	23.958740234375	0.0
18	0.0	0.0	0.0	25.814208984375	0.0
19	0.0	0.0	0.0	26.785888671875	0.0
20	0.0	0.0	0.0	27.697550455729168	0.0
21	0.0	0.0	0.0	28.855387369791668	0.0
22	0.0	0.0	0.0	30.041707356770832	0.0
23	0.0	0.0	0.0	31.147054036458332	0.0
24	0.0	0.0	0.0	32.001953125	0.0
25	0.0	0.0	0.0	32.7569580078125	0.0
26	0.0	0.0	0.0	33.408203125	0.0
27	0.0	0.0	0.0	34.088541666666664	0.0
28	2.0345052083333334E-4	0.0	0.0	34.791056315104164	0.0
29	2.0345052083333334E-4	0.0	0.0	35.536295572916664	0.0
30	2.0345052083333334E-4	0.0	0.0	36.353759765625	0.0
31	2.0345052083333334E-4	0.0	0.0	37.119547526041664	0.0
32	2.0345052083333334E-4	0.0	0.0	37.816975911458336	0.0
33	2.0345052083333334E-4	0.0	0.0	38.502197265625	0.0
34	2.0345052083333334E-4	0.0	0.0	39.136555989583336	0.0
35	2.0345052083333334E-4	0.0	0.0	39.815266927083336	0.0
36	2.0345052083333334E-4	0.0	0.0	40.522867838541664	0.0
37	2.0345052083333334E-4	0.0	0.0	41.168009440104164	0.0
38	2.0345052083333334E-4	0.0	0.0	41.806640625	0.0
39	2.0345052083333334E-4	0.0	0.0	42.427164713541664	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCTA	20	7.02934E-4	45.000004	37
AAGTACG	20	7.02934E-4	45.000004	1
TCGTTAG	20	7.02934E-4	45.000004	1
GTACACG	20	7.02934E-4	45.000004	1
CGTGCGA	20	7.02934E-4	45.000004	2
TAGCGCG	20	7.02934E-4	45.000004	1
GAATCGT	20	7.02934E-4	45.000004	45
GTTAGCG	35	1.2100281E-7	45.000004	1
GTCTACG	20	7.02934E-4	45.000004	1
TCTAGCG	20	7.02934E-4	45.000004	1
ACGATTA	20	7.02934E-4	45.000004	11
AATGCCG	20	7.02934E-4	45.000004	37
ATATCGG	20	7.02934E-4	45.000004	2
TCGTGGT	20	7.02934E-4	45.000004	1
TCATGCG	35	1.2100281E-7	45.000004	1
ACTACGG	20	7.02934E-4	45.000004	2
TCCACGG	35	1.2100281E-7	45.000004	2
TATTACG	20	7.02934E-4	45.000004	1
CTCGCCT	20	7.02934E-4	45.000004	36
AATTACG	20	7.02934E-4	45.000004	1
>>END_MODULE