FastQCFastQC Report
Sat 14 Jan 2017
SRR2934741.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934741.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3395809
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188150.5540653199281821No Hit
CGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTG86130.25363617329478777Illumina PCR Primer Index 8 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTT80630.23743973821849224No Hit
GCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC79040.2327574960782541RNA PCR Primer, Index 40 (96% over 27bp)
CGTTCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC74530.21947641931569176RNA PCR Primer, Index 40 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC68400.20142475622156605Illumina PCR Primer Index 8 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGCT65180.1919424796859894Illumina PCR Primer Index 8 (95% over 24bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCT55940.16473246875781294No Hit
CGTCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCT37890.11157871364378856Illumina PCR Primer Index 8 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCATA302.1667602E-645.00000412
CGTTTTT151650.043.560831
ACGTACG451.9301297E-840.01
TTGTACG1900.039.0789451
TTGCGAA750.039.01
CTTACGA301.14039154E-437.50000432
CGCACGG3050.036.8852462
TATTGCG1650.036.818181
GTTACGT250.00210768736.00000445
CGTTCTG9950.035.9547731
GTTTTTT187300.035.774162
CTAGACG1700.035.7352941
CGTAAGG4000.034.8750042
ACGTAGG4800.034.6875042
CGTTTTC7900.034.4620251
CGTTTCT11200.034.352681
GACCGAT14600.034.2123269
TAGGGAT28150.034.0497325
TTACACG9750.033.9230834
TACGGGA9950.033.9195984
Produced by FastQC (version 0.11.5)