##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934741.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3395809 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.21358827896386 33.0 31.0 34.0 30.0 34.0 2 32.40697606961994 34.0 31.0 34.0 30.0 34.0 3 32.37541245694325 34.0 31.0 34.0 30.0 34.0 4 35.94973598338422 37.0 35.0 37.0 35.0 37.0 5 35.84820347669731 37.0 35.0 37.0 35.0 37.0 6 35.78391305282482 37.0 35.0 37.0 35.0 37.0 7 36.098549417826504 37.0 35.0 37.0 35.0 37.0 8 35.953680551526894 37.0 35.0 37.0 35.0 37.0 9 37.676414662897706 39.0 38.0 39.0 35.0 39.0 10 37.33265004009354 39.0 37.0 39.0 34.0 39.0 11 37.23881231247105 39.0 37.0 39.0 34.0 39.0 12 37.19495383868763 39.0 37.0 39.0 34.0 39.0 13 37.19471678177424 39.0 37.0 39.0 34.0 39.0 14 38.34995342788714 40.0 38.0 41.0 34.0 41.0 15 38.419008253997795 40.0 38.0 41.0 34.0 41.0 16 38.486076808206825 40.0 38.0 41.0 34.0 41.0 17 38.47695527045249 40.0 38.0 41.0 34.0 41.0 18 38.408710266095646 40.0 38.0 41.0 34.0 41.0 19 38.30862395382072 40.0 37.0 41.0 34.0 41.0 20 38.22690852165125 40.0 37.0 41.0 34.0 41.0 21 38.16582823121088 40.0 37.0 41.0 34.0 41.0 22 38.12548438383902 40.0 37.0 41.0 34.0 41.0 23 37.94660977693386 40.0 36.0 41.0 34.0 41.0 24 37.70813582271559 39.0 36.0 41.0 33.0 41.0 25 37.62623575118624 39.0 36.0 41.0 33.0 41.0 26 37.80004205183507 40.0 36.0 41.0 33.0 41.0 27 37.884325355165736 40.0 36.0 41.0 34.0 41.0 28 37.825231336627 40.0 36.0 41.0 33.0 41.0 29 37.82797707409339 40.0 36.0 41.0 33.0 41.0 30 37.677818157617224 40.0 36.0 41.0 33.0 41.0 31 37.52438815021693 40.0 36.0 41.0 33.0 41.0 32 37.366634283612534 40.0 36.0 41.0 33.0 41.0 33 37.14390885942054 40.0 36.0 41.0 32.0 41.0 34 36.96927389025708 40.0 35.0 41.0 31.0 41.0 35 36.865079278604895 40.0 35.0 41.0 31.0 41.0 36 36.78169885290957 40.0 35.0 41.0 31.0 41.0 37 36.70982290228926 39.0 35.0 41.0 31.0 41.0 38 36.63193542392991 39.0 35.0 41.0 31.0 41.0 39 36.61665394019511 39.0 35.0 41.0 31.0 41.0 40 36.53815953724135 39.0 35.0 41.0 30.0 41.0 41 36.49463441553986 39.0 35.0 41.0 30.0 41.0 42 36.4054983657797 39.0 35.0 41.0 30.0 41.0 43 36.36135748506468 39.0 35.0 41.0 30.0 41.0 44 36.308135410442695 39.0 35.0 41.0 30.0 41.0 45 36.240170751653 39.0 35.0 41.0 30.0 41.0 46 36.18503072463734 39.0 35.0 41.0 30.0 41.0 47 36.075243925674265 39.0 35.0 41.0 30.0 41.0 48 36.017329891051 39.0 35.0 41.0 29.0 41.0 49 35.99883326771323 39.0 35.0 41.0 29.0 41.0 50 35.89869924957499 39.0 35.0 40.0 29.0 41.0 51 34.97008518441408 38.0 34.0 40.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 3.0 8 19.0 9 46.0 10 61.0 11 57.0 12 47.0 13 54.0 14 65.0 15 91.0 16 177.0 17 364.0 18 695.0 19 1394.0 20 2383.0 21 3922.0 22 6357.0 23 9932.0 24 16223.0 25 27336.0 26 38244.0 27 41460.0 28 40268.0 29 41739.0 30 47807.0 31 58562.0 32 75337.0 33 105761.0 34 184131.0 35 238694.0 36 256680.0 37 400412.0 38 734686.0 39 1062163.0 40 639.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.554109197543205 21.592115457612604 25.290232754551273 13.563542590292919 2 35.10630309301848 23.068876959805454 25.033975703580502 16.79084424359556 3 30.15140133028683 23.582657328489322 29.7216068394895 16.544334501734344 4 26.662748110980328 25.38487883152439 29.83085915609506 18.121513901400228 5 24.09207938373448 29.659589217179178 28.278121649362493 17.97020974972385 6 25.741082610947792 32.91336467981562 26.824211844659107 14.521340864577484 7 78.96377564226962 5.467121384035439 10.982537592662013 4.586565381032914 8 80.89168148149675 3.7991830518147514 10.575771487736796 4.733363978951702 9 75.41663856830581 5.856630923588459 12.455529742691652 6.271200765414074 10 38.36626264904769 24.841414814555236 21.681990948254157 15.110331588142914 11 27.01762672753385 24.844153484486316 28.110061549398097 20.028158238581735 12 23.492575701401343 22.285852944026004 33.06310808411192 21.158463270460736 13 22.671917060117337 23.70024344714323 34.75793249855925 18.86990699418018 14 20.74144923934179 26.334225511505505 32.89207372970623 20.03225151944647 15 19.659910201074325 25.44901671442652 35.0512057656953 19.839867318803854 16 22.46613399045706 25.25262757711049 31.93127175291661 20.349966679515838 17 22.911123682162334 25.401134162728233 29.723697652017535 21.964044503091902 18 23.566549237604352 24.935795858954375 31.440578666232405 20.057076237208864 19 22.294186746074352 26.95578579360618 29.6765807499774 21.073446710342072 20 22.408297993202797 27.223468693321678 30.175342606135974 20.192890707339547 21 22.27260131532722 26.883755829612326 31.388661729796937 19.454981125263522 22 21.79922369014276 24.350515591424607 30.649132504213284 23.201128214219352 23 20.298196983399244 26.35875574863015 30.906979750627904 22.4360675173427 24 21.062109205788666 24.94062534141349 31.55572059559298 22.441544857204867 25 20.49040449565921 27.144812914978434 29.937549491152183 22.427233098210174 26 19.8909891575174 26.763784417792635 30.744691471163428 22.600534953526537 27 21.147420246545078 26.02348954255083 30.659498222662112 22.169591988241976 28 19.507663711357146 26.094312135929908 31.1623828077492 23.23564134496375 29 20.465609225960588 25.080297507898706 30.916785955865013 23.537307310275697 30 21.652012819331123 25.63819107611765 29.956749628733537 22.753046475817694 31 20.892341118125312 27.96205558086453 28.09056693117899 23.055036369831168 32 20.790715850037504 27.645577239473717 28.505872974598983 23.0578339358898 33 20.866721302640993 27.40224788850021 27.369383849327217 24.361646959531587 34 19.506898061698994 28.30212182133919 28.647606505548456 23.54337361141336 35 20.007043976854998 26.882195082232247 28.383015652529338 24.727745288383414 36 20.098450766812856 28.499453296696014 26.679975228288754 24.722120708202375 37 19.92806426981023 28.69970013036658 27.55685022331939 23.8153853765038 38 20.687647626824713 28.15146552706586 27.00299103983764 24.15789580627179 39 21.35691377224102 27.05596810656901 27.198290598793985 24.38882752239599 40 20.86362925594461 26.661864669067075 28.646163550423477 23.82834252456484 41 18.966437747234902 25.945746654184614 28.132383181739606 26.955432416840875 42 19.91230955569056 25.350277356588663 28.96308361277092 25.77432947494986 43 20.704109094474983 25.069961237513656 29.27214104209041 24.95378862592095 44 20.777994286486667 26.28725584978425 27.70356047704685 25.231189386682228 45 20.90727128645928 25.278600769360114 28.34917982725177 25.464948116928838 46 21.23856200392896 26.419566000325695 27.358900338623286 24.982971657122057 47 19.062320642886572 25.780071847385997 30.060347917094276 25.097259592633154 48 19.372290962183094 25.591427550842816 29.042416696580993 25.9938647903931 49 21.00124005796557 24.302309111024794 29.47344800605688 25.223002824952758 50 19.63004986440639 24.226833723569257 30.12607599544026 26.01704041658409 51 19.398146362177613 24.085571361640188 27.929692158775715 28.586590117406484 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 597.0 1 1331.5 2 2066.0 3 17668.0 4 33270.0 5 23377.0 6 13484.0 7 13307.5 8 13131.0 9 13400.0 10 13669.0 11 13656.5 12 13644.0 13 13791.0 14 13938.0 15 13406.5 16 12875.0 17 12385.0 18 11895.0 19 11973.5 20 12052.0 21 11718.0 22 11384.0 23 12449.5 24 13515.0 25 15960.0 26 20948.0 27 23491.0 28 29781.5 29 36072.0 30 40560.0 31 45048.0 32 54112.5 33 63177.0 34 72567.0 35 81957.0 36 92792.5 37 103628.0 38 115109.5 39 126591.0 40 142643.5 41 158696.0 42 177748.5 43 196801.0 44 219228.5 45 241656.0 46 284178.0 47 326700.0 48 357784.0 49 388868.0 50 380582.0 51 372296.0 52 316812.0 53 261328.0 54 229738.5 55 198149.0 56 174682.0 57 151215.0 58 134525.0 59 117835.0 60 106482.0 61 95129.0 62 83535.5 63 71942.0 64 62239.0 65 52536.0 66 44002.5 67 35469.0 68 29814.0 69 24159.0 70 20314.0 71 16469.0 72 13158.5 73 9848.0 74 8183.0 75 4782.5 76 3047.0 77 2339.0 78 1631.0 79 1245.0 80 859.0 81 660.5 82 462.0 83 332.0 84 202.0 85 124.0 86 46.0 87 35.5 88 25.0 89 19.0 90 13.0 91 9.5 92 6.0 93 8.0 94 10.0 95 7.5 96 5.0 97 2.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 3395809.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.5909549228332 #Duplication Level Percentage of deduplicated Percentage of total 1 69.88709247058675 18.583645255732478 2 12.392709792822181 6.590679749453764 3 5.040598478607819 4.021029807862863 4 2.721956731620128 2.895177150096528 5 1.6050278115551975 2.1339611093478936 6 1.0939944317429273 1.745421397218402 7 0.7809239759587805 1.4535859970015725 8 0.5766253353461166 1.226641463964173 9 0.4643404901844222 1.111253133900622 >10 3.952130474585174 23.76693706299499 >50 1.1809788794388452 22.34604107734634 >100 0.29647603647511017 10.380738917143129 >500 0.00453656576286214 0.8339866751746517 >1k 0.0017012121610729912 0.8058795087153384 >5k 7.938990085007292E-4 1.5376007316762044 >10k+ 1.1341414407153275E-4 0.5674209623710461 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18815 0.5540653199281821 No Hit CGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTG 8613 0.25363617329478777 Illumina PCR Primer Index 8 (95% over 22bp) CGTTTCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTT 8063 0.23743973821849224 No Hit GCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC 7904 0.2327574960782541 RNA PCR Primer, Index 40 (96% over 27bp) CGTTCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC 7453 0.21947641931569176 RNA PCR Primer, Index 40 (95% over 24bp) CCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC 6840 0.20142475622156605 Illumina PCR Primer Index 8 (95% over 23bp) CTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGCT 6518 0.1919424796859894 Illumina PCR Primer Index 8 (95% over 24bp) CGTTTTCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCT 5594 0.16473246875781294 No Hit CGTCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCT 3789 0.11157871364378856 Illumina PCR Primer Index 8 (95% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.061364464255793E-4 0.0 0.0 0.20404563389754843 0.0 2 2.061364464255793E-4 0.0 0.0 0.804903927164337 0.0 3 2.061364464255793E-4 0.0 0.0 1.3719558432173304 0.0 4 2.061364464255793E-4 0.0 0.0 1.9302027882015744 0.0 5 2.061364464255793E-4 0.0 0.0 3.4453351174933573 0.0 6 2.061364464255793E-4 0.0 0.0 4.664691094228209 0.0 7 2.3558451020066205E-4 0.0 0.0 5.744728281243144 0.0 8 2.3558451020066205E-4 0.0 0.0 7.299350464057313 0.0 9 2.3558451020066205E-4 0.0 0.0 7.927065391486977 0.0 10 2.3558451020066205E-4 0.0 0.0 9.204728534496493 0.0 11 2.3558451020066205E-4 0.0 0.0 10.908799640969207 0.0 12 2.3558451020066205E-4 0.0 0.0 12.22480416301388 0.0 13 2.3558451020066205E-4 0.0 0.0 12.801662284303976 0.0 14 2.3558451020066205E-4 0.0 0.0 13.043902056917807 0.0 15 2.3558451020066205E-4 0.0 0.0 13.439477897608493 0.0 16 2.3558451020066205E-4 0.0 0.0 14.264465404267437 0.0 17 2.3558451020066205E-4 0.0 0.0 15.319707321583753 0.0 18 2.3558451020066205E-4 0.0 0.0 16.470066484893586 0.0 19 2.3558451020066205E-4 0.0 0.0 17.165659199324814 0.0 20 2.3558451020066205E-4 0.0 0.0 17.831185440641686 0.0 21 2.3558451020066205E-4 0.0 0.0 18.695780593078116 0.0 22 2.3558451020066205E-4 0.0 0.0 19.63823642613586 0.0 23 2.3558451020066205E-4 0.0 0.0 20.619298670802745 0.0 24 2.3558451020066205E-4 0.0 0.0 21.35862175993997 0.0 25 2.3558451020066205E-4 0.0 0.0 22.047176387128957 0.0 26 2.650325739757448E-4 0.0 0.0 22.684108558520222 0.0 27 2.650325739757448E-4 0.0 0.0 23.334056774100073 0.0 28 2.650325739757448E-4 0.0 0.0 23.983798853233502 0.0 29 2.650325739757448E-4 0.0 0.0 24.664049126437913 0.0 30 2.650325739757448E-4 0.0 0.0 25.433585929008373 0.0 31 2.944806377508276E-4 0.0 0.0 26.16077641587027 0.0 32 2.944806377508276E-4 0.0 0.0 26.856958091577 0.0 33 2.944806377508276E-4 0.0 0.0 27.53058255043202 0.0 34 2.944806377508276E-4 0.0 0.0 28.17843995348384 0.0 35 2.944806377508276E-4 0.0 0.0 28.86083993534383 0.0 36 3.239287015259103E-4 0.0 0.0 29.49456226778361 0.0 37 3.239287015259103E-4 0.0 0.0 30.170660363995736 0.0 38 3.239287015259103E-4 0.0 0.0 30.856623561572516 0.0 39 3.239287015259103E-4 0.0 0.0 31.614322242505395 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCATA 30 2.1667602E-6 45.000004 12 CGTTTTT 15165 0.0 43.56083 1 ACGTACG 45 1.9301297E-8 40.0 1 TTGTACG 190 0.0 39.078945 1 TTGCGAA 75 0.0 39.0 1 CTTACGA 30 1.14039154E-4 37.500004 32 CGCACGG 305 0.0 36.885246 2 TATTGCG 165 0.0 36.81818 1 GTTACGT 25 0.002107687 36.000004 45 CGTTCTG 995 0.0 35.954773 1 GTTTTTT 18730 0.0 35.77416 2 CTAGACG 170 0.0 35.735294 1 CGTAAGG 400 0.0 34.875004 2 ACGTAGG 480 0.0 34.687504 2 CGTTTTC 790 0.0 34.462025 1 CGTTTCT 1120 0.0 34.35268 1 GACCGAT 1460 0.0 34.212326 9 TAGGGAT 2815 0.0 34.049732 5 TTACACG 975 0.0 33.92308 34 TACGGGA 995 0.0 33.919598 4 >>END_MODULE