##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934741.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3395809
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21358827896386	33.0	31.0	34.0	30.0	34.0
2	32.40697606961994	34.0	31.0	34.0	30.0	34.0
3	32.37541245694325	34.0	31.0	34.0	30.0	34.0
4	35.94973598338422	37.0	35.0	37.0	35.0	37.0
5	35.84820347669731	37.0	35.0	37.0	35.0	37.0
6	35.78391305282482	37.0	35.0	37.0	35.0	37.0
7	36.098549417826504	37.0	35.0	37.0	35.0	37.0
8	35.953680551526894	37.0	35.0	37.0	35.0	37.0
9	37.676414662897706	39.0	38.0	39.0	35.0	39.0
10	37.33265004009354	39.0	37.0	39.0	34.0	39.0
11	37.23881231247105	39.0	37.0	39.0	34.0	39.0
12	37.19495383868763	39.0	37.0	39.0	34.0	39.0
13	37.19471678177424	39.0	37.0	39.0	34.0	39.0
14	38.34995342788714	40.0	38.0	41.0	34.0	41.0
15	38.419008253997795	40.0	38.0	41.0	34.0	41.0
16	38.486076808206825	40.0	38.0	41.0	34.0	41.0
17	38.47695527045249	40.0	38.0	41.0	34.0	41.0
18	38.408710266095646	40.0	38.0	41.0	34.0	41.0
19	38.30862395382072	40.0	37.0	41.0	34.0	41.0
20	38.22690852165125	40.0	37.0	41.0	34.0	41.0
21	38.16582823121088	40.0	37.0	41.0	34.0	41.0
22	38.12548438383902	40.0	37.0	41.0	34.0	41.0
23	37.94660977693386	40.0	36.0	41.0	34.0	41.0
24	37.70813582271559	39.0	36.0	41.0	33.0	41.0
25	37.62623575118624	39.0	36.0	41.0	33.0	41.0
26	37.80004205183507	40.0	36.0	41.0	33.0	41.0
27	37.884325355165736	40.0	36.0	41.0	34.0	41.0
28	37.825231336627	40.0	36.0	41.0	33.0	41.0
29	37.82797707409339	40.0	36.0	41.0	33.0	41.0
30	37.677818157617224	40.0	36.0	41.0	33.0	41.0
31	37.52438815021693	40.0	36.0	41.0	33.0	41.0
32	37.366634283612534	40.0	36.0	41.0	33.0	41.0
33	37.14390885942054	40.0	36.0	41.0	32.0	41.0
34	36.96927389025708	40.0	35.0	41.0	31.0	41.0
35	36.865079278604895	40.0	35.0	41.0	31.0	41.0
36	36.78169885290957	40.0	35.0	41.0	31.0	41.0
37	36.70982290228926	39.0	35.0	41.0	31.0	41.0
38	36.63193542392991	39.0	35.0	41.0	31.0	41.0
39	36.61665394019511	39.0	35.0	41.0	31.0	41.0
40	36.53815953724135	39.0	35.0	41.0	30.0	41.0
41	36.49463441553986	39.0	35.0	41.0	30.0	41.0
42	36.4054983657797	39.0	35.0	41.0	30.0	41.0
43	36.36135748506468	39.0	35.0	41.0	30.0	41.0
44	36.308135410442695	39.0	35.0	41.0	30.0	41.0
45	36.240170751653	39.0	35.0	41.0	30.0	41.0
46	36.18503072463734	39.0	35.0	41.0	30.0	41.0
47	36.075243925674265	39.0	35.0	41.0	30.0	41.0
48	36.017329891051	39.0	35.0	41.0	29.0	41.0
49	35.99883326771323	39.0	35.0	41.0	29.0	41.0
50	35.89869924957499	39.0	35.0	40.0	29.0	41.0
51	34.97008518441408	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	19.0
9	46.0
10	61.0
11	57.0
12	47.0
13	54.0
14	65.0
15	91.0
16	177.0
17	364.0
18	695.0
19	1394.0
20	2383.0
21	3922.0
22	6357.0
23	9932.0
24	16223.0
25	27336.0
26	38244.0
27	41460.0
28	40268.0
29	41739.0
30	47807.0
31	58562.0
32	75337.0
33	105761.0
34	184131.0
35	238694.0
36	256680.0
37	400412.0
38	734686.0
39	1062163.0
40	639.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.554109197543205	21.592115457612604	25.290232754551273	13.563542590292919
2	35.10630309301848	23.068876959805454	25.033975703580502	16.79084424359556
3	30.15140133028683	23.582657328489322	29.7216068394895	16.544334501734344
4	26.662748110980328	25.38487883152439	29.83085915609506	18.121513901400228
5	24.09207938373448	29.659589217179178	28.278121649362493	17.97020974972385
6	25.741082610947792	32.91336467981562	26.824211844659107	14.521340864577484
7	78.96377564226962	5.467121384035439	10.982537592662013	4.586565381032914
8	80.89168148149675	3.7991830518147514	10.575771487736796	4.733363978951702
9	75.41663856830581	5.856630923588459	12.455529742691652	6.271200765414074
10	38.36626264904769	24.841414814555236	21.681990948254157	15.110331588142914
11	27.01762672753385	24.844153484486316	28.110061549398097	20.028158238581735
12	23.492575701401343	22.285852944026004	33.06310808411192	21.158463270460736
13	22.671917060117337	23.70024344714323	34.75793249855925	18.86990699418018
14	20.74144923934179	26.334225511505505	32.89207372970623	20.03225151944647
15	19.659910201074325	25.44901671442652	35.0512057656953	19.839867318803854
16	22.46613399045706	25.25262757711049	31.93127175291661	20.349966679515838
17	22.911123682162334	25.401134162728233	29.723697652017535	21.964044503091902
18	23.566549237604352	24.935795858954375	31.440578666232405	20.057076237208864
19	22.294186746074352	26.95578579360618	29.6765807499774	21.073446710342072
20	22.408297993202797	27.223468693321678	30.175342606135974	20.192890707339547
21	22.27260131532722	26.883755829612326	31.388661729796937	19.454981125263522
22	21.79922369014276	24.350515591424607	30.649132504213284	23.201128214219352
23	20.298196983399244	26.35875574863015	30.906979750627904	22.4360675173427
24	21.062109205788666	24.94062534141349	31.55572059559298	22.441544857204867
25	20.49040449565921	27.144812914978434	29.937549491152183	22.427233098210174
26	19.8909891575174	26.763784417792635	30.744691471163428	22.600534953526537
27	21.147420246545078	26.02348954255083	30.659498222662112	22.169591988241976
28	19.507663711357146	26.094312135929908	31.1623828077492	23.23564134496375
29	20.465609225960588	25.080297507898706	30.916785955865013	23.537307310275697
30	21.652012819331123	25.63819107611765	29.956749628733537	22.753046475817694
31	20.892341118125312	27.96205558086453	28.09056693117899	23.055036369831168
32	20.790715850037504	27.645577239473717	28.505872974598983	23.0578339358898
33	20.866721302640993	27.40224788850021	27.369383849327217	24.361646959531587
34	19.506898061698994	28.30212182133919	28.647606505548456	23.54337361141336
35	20.007043976854998	26.882195082232247	28.383015652529338	24.727745288383414
36	20.098450766812856	28.499453296696014	26.679975228288754	24.722120708202375
37	19.92806426981023	28.69970013036658	27.55685022331939	23.8153853765038
38	20.687647626824713	28.15146552706586	27.00299103983764	24.15789580627179
39	21.35691377224102	27.05596810656901	27.198290598793985	24.38882752239599
40	20.86362925594461	26.661864669067075	28.646163550423477	23.82834252456484
41	18.966437747234902	25.945746654184614	28.132383181739606	26.955432416840875
42	19.91230955569056	25.350277356588663	28.96308361277092	25.77432947494986
43	20.704109094474983	25.069961237513656	29.27214104209041	24.95378862592095
44	20.777994286486667	26.28725584978425	27.70356047704685	25.231189386682228
45	20.90727128645928	25.278600769360114	28.34917982725177	25.464948116928838
46	21.23856200392896	26.419566000325695	27.358900338623286	24.982971657122057
47	19.062320642886572	25.780071847385997	30.060347917094276	25.097259592633154
48	19.372290962183094	25.591427550842816	29.042416696580993	25.9938647903931
49	21.00124005796557	24.302309111024794	29.47344800605688	25.223002824952758
50	19.63004986440639	24.226833723569257	30.12607599544026	26.01704041658409
51	19.398146362177613	24.085571361640188	27.929692158775715	28.586590117406484
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	597.0
1	1331.5
2	2066.0
3	17668.0
4	33270.0
5	23377.0
6	13484.0
7	13307.5
8	13131.0
9	13400.0
10	13669.0
11	13656.5
12	13644.0
13	13791.0
14	13938.0
15	13406.5
16	12875.0
17	12385.0
18	11895.0
19	11973.5
20	12052.0
21	11718.0
22	11384.0
23	12449.5
24	13515.0
25	15960.0
26	20948.0
27	23491.0
28	29781.5
29	36072.0
30	40560.0
31	45048.0
32	54112.5
33	63177.0
34	72567.0
35	81957.0
36	92792.5
37	103628.0
38	115109.5
39	126591.0
40	142643.5
41	158696.0
42	177748.5
43	196801.0
44	219228.5
45	241656.0
46	284178.0
47	326700.0
48	357784.0
49	388868.0
50	380582.0
51	372296.0
52	316812.0
53	261328.0
54	229738.5
55	198149.0
56	174682.0
57	151215.0
58	134525.0
59	117835.0
60	106482.0
61	95129.0
62	83535.5
63	71942.0
64	62239.0
65	52536.0
66	44002.5
67	35469.0
68	29814.0
69	24159.0
70	20314.0
71	16469.0
72	13158.5
73	9848.0
74	8183.0
75	4782.5
76	3047.0
77	2339.0
78	1631.0
79	1245.0
80	859.0
81	660.5
82	462.0
83	332.0
84	202.0
85	124.0
86	46.0
87	35.5
88	25.0
89	19.0
90	13.0
91	9.5
92	6.0
93	8.0
94	10.0
95	7.5
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3395809.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.5909549228332
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.88709247058675	18.583645255732478
2	12.392709792822181	6.590679749453764
3	5.040598478607819	4.021029807862863
4	2.721956731620128	2.895177150096528
5	1.6050278115551975	2.1339611093478936
6	1.0939944317429273	1.745421397218402
7	0.7809239759587805	1.4535859970015725
8	0.5766253353461166	1.226641463964173
9	0.4643404901844222	1.111253133900622
>10	3.952130474585174	23.76693706299499
>50	1.1809788794388452	22.34604107734634
>100	0.29647603647511017	10.380738917143129
>500	0.00453656576286214	0.8339866751746517
>1k	0.0017012121610729912	0.8058795087153384
>5k	7.938990085007292E-4	1.5376007316762044
>10k+	1.1341414407153275E-4	0.5674209623710461
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18815	0.5540653199281821	No Hit
CGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTG	8613	0.25363617329478777	Illumina PCR Primer Index 8 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTT	8063	0.23743973821849224	No Hit
GCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	7904	0.2327574960782541	RNA PCR Primer, Index 40 (96% over 27bp)
CGTTCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC	7453	0.21947641931569176	RNA PCR Primer, Index 40 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	6840	0.20142475622156605	Illumina PCR Primer Index 8 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGCT	6518	0.1919424796859894	Illumina PCR Primer Index 8 (95% over 24bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCT	5594	0.16473246875781294	No Hit
CGTCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCT	3789	0.11157871364378856	Illumina PCR Primer Index 8 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.061364464255793E-4	0.0	0.0	0.20404563389754843	0.0
2	2.061364464255793E-4	0.0	0.0	0.804903927164337	0.0
3	2.061364464255793E-4	0.0	0.0	1.3719558432173304	0.0
4	2.061364464255793E-4	0.0	0.0	1.9302027882015744	0.0
5	2.061364464255793E-4	0.0	0.0	3.4453351174933573	0.0
6	2.061364464255793E-4	0.0	0.0	4.664691094228209	0.0
7	2.3558451020066205E-4	0.0	0.0	5.744728281243144	0.0
8	2.3558451020066205E-4	0.0	0.0	7.299350464057313	0.0
9	2.3558451020066205E-4	0.0	0.0	7.927065391486977	0.0
10	2.3558451020066205E-4	0.0	0.0	9.204728534496493	0.0
11	2.3558451020066205E-4	0.0	0.0	10.908799640969207	0.0
12	2.3558451020066205E-4	0.0	0.0	12.22480416301388	0.0
13	2.3558451020066205E-4	0.0	0.0	12.801662284303976	0.0
14	2.3558451020066205E-4	0.0	0.0	13.043902056917807	0.0
15	2.3558451020066205E-4	0.0	0.0	13.439477897608493	0.0
16	2.3558451020066205E-4	0.0	0.0	14.264465404267437	0.0
17	2.3558451020066205E-4	0.0	0.0	15.319707321583753	0.0
18	2.3558451020066205E-4	0.0	0.0	16.470066484893586	0.0
19	2.3558451020066205E-4	0.0	0.0	17.165659199324814	0.0
20	2.3558451020066205E-4	0.0	0.0	17.831185440641686	0.0
21	2.3558451020066205E-4	0.0	0.0	18.695780593078116	0.0
22	2.3558451020066205E-4	0.0	0.0	19.63823642613586	0.0
23	2.3558451020066205E-4	0.0	0.0	20.619298670802745	0.0
24	2.3558451020066205E-4	0.0	0.0	21.35862175993997	0.0
25	2.3558451020066205E-4	0.0	0.0	22.047176387128957	0.0
26	2.650325739757448E-4	0.0	0.0	22.684108558520222	0.0
27	2.650325739757448E-4	0.0	0.0	23.334056774100073	0.0
28	2.650325739757448E-4	0.0	0.0	23.983798853233502	0.0
29	2.650325739757448E-4	0.0	0.0	24.664049126437913	0.0
30	2.650325739757448E-4	0.0	0.0	25.433585929008373	0.0
31	2.944806377508276E-4	0.0	0.0	26.16077641587027	0.0
32	2.944806377508276E-4	0.0	0.0	26.856958091577	0.0
33	2.944806377508276E-4	0.0	0.0	27.53058255043202	0.0
34	2.944806377508276E-4	0.0	0.0	28.17843995348384	0.0
35	2.944806377508276E-4	0.0	0.0	28.86083993534383	0.0
36	3.239287015259103E-4	0.0	0.0	29.49456226778361	0.0
37	3.239287015259103E-4	0.0	0.0	30.170660363995736	0.0
38	3.239287015259103E-4	0.0	0.0	30.856623561572516	0.0
39	3.239287015259103E-4	0.0	0.0	31.614322242505395	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCATA	30	2.1667602E-6	45.000004	12
CGTTTTT	15165	0.0	43.56083	1
ACGTACG	45	1.9301297E-8	40.0	1
TTGTACG	190	0.0	39.078945	1
TTGCGAA	75	0.0	39.0	1
CTTACGA	30	1.14039154E-4	37.500004	32
CGCACGG	305	0.0	36.885246	2
TATTGCG	165	0.0	36.81818	1
GTTACGT	25	0.002107687	36.000004	45
CGTTCTG	995	0.0	35.954773	1
GTTTTTT	18730	0.0	35.77416	2
CTAGACG	170	0.0	35.735294	1
CGTAAGG	400	0.0	34.875004	2
ACGTAGG	480	0.0	34.687504	2
CGTTTTC	790	0.0	34.462025	1
CGTTTCT	1120	0.0	34.35268	1
GACCGAT	1460	0.0	34.212326	9
TAGGGAT	2815	0.0	34.049732	5
TTACACG	975	0.0	33.92308	34
TACGGGA	995	0.0	33.919598	4
>>END_MODULE