##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934739.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1813396
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19983941731425	33.0	31.0	34.0	30.0	34.0
2	32.37937494071896	34.0	31.0	34.0	30.0	34.0
3	32.3814202744464	34.0	31.0	34.0	30.0	34.0
4	35.93840120966408	37.0	35.0	37.0	35.0	37.0
5	35.8256525325963	37.0	35.0	37.0	35.0	37.0
6	35.76062812535155	37.0	35.0	37.0	35.0	37.0
7	36.08328241597533	37.0	35.0	37.0	35.0	37.0
8	35.917986473996855	37.0	35.0	37.0	35.0	37.0
9	37.58848205245848	39.0	38.0	39.0	35.0	39.0
10	37.341961711617316	39.0	37.0	39.0	34.0	39.0
11	37.21596441152401	39.0	37.0	39.0	34.0	39.0
12	37.12979183807619	39.0	37.0	39.0	34.0	39.0
13	37.05210058917081	39.0	37.0	39.0	33.0	39.0
14	38.19777367988018	40.0	37.0	41.0	33.0	41.0
15	38.2754908470075	40.0	37.0	41.0	34.0	41.0
16	38.36188455251914	40.0	37.0	41.0	34.0	41.0
17	38.36659725730067	40.0	37.0	41.0	34.0	41.0
18	38.34598179327626	40.0	37.0	41.0	34.0	41.0
19	38.343262034326756	40.0	37.0	41.0	34.0	41.0
20	38.2858669590095	40.0	37.0	41.0	34.0	41.0
21	38.221347129915365	40.0	37.0	41.0	34.0	41.0
22	38.14980346267445	40.0	37.0	41.0	34.0	41.0
23	37.981240170376466	40.0	36.0	41.0	34.0	41.0
24	37.76030221749689	40.0	36.0	41.0	33.0	41.0
25	37.69973133281423	39.0	36.0	41.0	33.0	41.0
26	37.877898153519695	40.0	36.0	41.0	34.0	41.0
27	37.97418986255622	40.0	36.0	41.0	34.0	41.0
28	37.93992873040417	40.0	36.0	41.0	34.0	41.0
29	37.96188146439057	40.0	36.0	41.0	34.0	41.0
30	37.85081912610373	40.0	36.0	41.0	34.0	41.0
31	37.75386291797269	40.0	36.0	41.0	33.0	41.0
32	37.66635858907817	40.0	36.0	41.0	33.0	41.0
33	37.56419281833642	40.0	36.0	41.0	33.0	41.0
34	37.483035696560485	40.0	36.0	41.0	33.0	41.0
35	37.41475165931766	40.0	36.0	41.0	33.0	41.0
36	37.35032281972608	40.0	36.0	41.0	33.0	41.0
37	37.253465321419036	39.0	35.0	41.0	33.0	41.0
38	37.167034668654836	39.0	35.0	41.0	32.0	41.0
39	37.213047232926506	39.0	35.0	41.0	33.0	41.0
40	37.1422414078337	39.0	35.0	41.0	32.0	41.0
41	37.10242660731578	39.0	35.0	41.0	32.0	41.0
42	37.00387284410024	39.0	35.0	41.0	32.0	41.0
43	36.93568310506916	39.0	35.0	41.0	32.0	41.0
44	36.87967768760932	39.0	35.0	41.0	32.0	41.0
45	36.84667827655956	39.0	35.0	41.0	31.0	41.0
46	36.815843864219396	39.0	35.0	41.0	31.0	41.0
47	36.69487249337707	39.0	35.0	41.0	31.0	41.0
48	36.668142534780046	39.0	35.0	41.0	31.0	41.0
49	36.65315573652969	39.0	35.0	41.0	31.0	41.0
50	36.53185239186587	39.0	35.0	40.0	31.0	41.0
51	35.60018164813422	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	28.0
10	32.0
11	21.0
12	29.0
13	32.0
14	18.0
15	47.0
16	74.0
17	188.0
18	296.0
19	598.0
20	983.0
21	1685.0
22	2585.0
23	3909.0
24	5589.0
25	8183.0
26	10995.0
27	13716.0
28	15869.0
29	18588.0
30	23270.0
31	29719.0
32	39697.0
33	57319.0
34	108407.0
35	149810.0
36	135085.0
37	211049.0
38	395993.0
39	579223.0
40	351.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.187191325005685	22.15660561730587	27.44315086169816	12.213052195990286
2	30.946357000897763	24.441103873616132	26.954233934562556	17.65830519092355
3	30.026756428270495	24.083983862322405	28.960138877553497	16.929120831853606
4	27.570260439528926	26.09005424077256	28.058074463603095	18.281610856095416
5	24.664993195088112	30.11642244716543	26.161577504306837	19.057006853439624
6	26.584595973521502	33.004870419919314	25.177677683197714	15.232855923361472
7	80.73437903248933	5.0986105627232	8.809217622626276	5.357792782161205
8	80.71281727763821	4.076881166606743	8.83695563462145	6.373345921133608
9	74.68556233718392	6.710062225790726	11.593165530308879	7.011209906716459
10	39.5310235602152	28.754723182360607	17.630787759540663	14.08346549788353
11	26.75488420620758	23.65032237856486	28.832919009416585	20.761874405810975
12	24.974192068362346	21.615907391435737	32.39888033281203	21.01102020738989
13	22.231933896402108	25.546984773320336	33.04738733293776	19.173693997339797
14	18.97864559092443	28.695111271889868	30.331047382921327	21.995195754264373
15	17.05816049004189	25.926604007067404	36.62503942878445	20.390196074106264
16	19.155826967744495	26.82811696948708	31.98099036283305	22.03506569993537
17	19.890911858193135	24.574886014968598	29.080355311250273	26.45384681558799
18	20.735184151724166	24.408347652691415	32.67681190429448	22.179656291289934
19	21.27632353881888	26.185455355586978	29.523060600111613	23.01516050548253
20	22.03341134534321	27.34201465096427	29.335291353901738	21.28928264979078
21	22.18610827419935	26.52900965922501	30.379905988543044	20.904976078032597
22	20.559160823118614	25.198357115599677	28.527635442010457	25.71484661927125
23	19.475448274949322	26.624907080417078	29.200185728875542	24.699458915758058
24	20.95923890865536	24.09098729676254	30.790075637091952	24.159698157490144
25	18.658307396729672	27.340415441525185	29.038941301293264	24.96233586045188
26	18.19481238516022	26.893188250111944	31.095248914191938	23.8167504505359
27	20.15384394804003	27.408795431334358	30.32806954465544	22.10929107597017
28	17.666190947812833	27.00783502334846	31.784011876060163	23.541962152778545
29	19.623788736712775	23.47622913031682	32.538673295849335	24.361308837121072
30	20.733143781060505	24.90812817498219	30.617030146752285	23.74169789720502
31	20.251064852905817	28.297514718241356	26.908849473584368	24.54257095526846
32	22.04747335937655	27.873834507189827	27.875819732700414	22.20287240073321
33	21.968670935636784	25.5525544337806	28.047982900590934	24.430791729991686
34	20.098367924049683	25.455774690139382	28.571475838702632	25.8743815471083
35	20.2281244692279	25.43051821003245	29.1496176235086	25.191739697231053
36	22.249690635691266	25.175085861003332	29.17774165157528	23.397481851730124
37	20.64540784252309	27.446128699964046	29.800275284604133	22.10818817290873
38	20.977381664016022	28.962840990054023	26.325910060461144	23.73386728546881
39	23.414521704029344	24.39704289631167	27.326629153257205	24.86180624640178
40	21.817848941985094	23.38187577341077	29.337442014871545	25.462833269732588
41	19.12770293967782	25.87939975603784	27.693399566338517	27.29949773794582
42	22.35060626581287	24.447004404994825	29.391870280953526	23.810519048238774
43	23.62523133391714	23.590600177787973	29.7074659919841	23.076702496310787
44	20.788674950203927	24.47325349785706	28.19979750699792	26.538274044941097
45	21.354795091640216	23.02084045624894	27.889054569437672	27.73530988267317
46	22.64271014163481	24.150544062080208	28.978502213526447	24.228243582758537
47	19.211799298112492	24.635435393041565	31.116479798124626	25.036285510721324
48	20.27097225316478	23.63251049412263	30.227595075758412	25.86892217695418
49	22.399299435975376	21.59622057178906	31.93648822430401	24.06799176793155
50	20.68307198207121	21.849777985613734	30.76625293096489	26.700897101350172
51	20.116290098798057	21.749634387635133	27.55129050687219	30.582785006694625
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	394.0
1	817.0
2	1240.0
3	5279.0
4	9318.0
5	6363.0
6	3408.0
7	3278.0
8	3148.0
9	3430.5
10	3713.0
11	3664.0
12	3615.0
13	3669.5
14	3724.0
15	3659.0
16	3594.0
17	3690.0
18	3786.0
19	3647.0
20	3508.0
21	4006.0
22	4504.0
23	5388.5
24	6273.0
25	7740.0
26	11313.0
27	13419.0
28	16209.0
29	18999.0
30	21671.5
31	24344.0
32	28300.5
33	32257.0
34	38819.5
35	45382.0
36	49426.5
37	53471.0
38	58380.5
39	63290.0
40	72450.5
41	81611.0
42	96438.5
43	111266.0
44	125385.5
45	139505.0
46	163134.5
47	186764.0
48	212583.0
49	238402.0
50	228609.5
51	218817.0
52	181004.0
53	143191.0
54	120417.0
55	97643.0
56	83534.5
57	69426.0
58	63768.0
59	58110.0
60	54663.0
61	51216.0
62	44196.0
63	37176.0
64	31713.5
65	26251.0
66	21703.0
67	17155.0
68	13749.5
69	10344.0
70	8368.0
71	6392.0
72	5531.0
73	4670.0
74	3363.5
75	1706.0
76	1355.0
77	1064.5
78	774.0
79	530.0
80	286.0
81	266.5
82	247.0
83	175.5
84	104.0
85	60.5
86	17.0
87	14.0
88	11.0
89	8.5
90	6.0
91	4.5
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1813396.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.563240218041294
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.97672357858251	14.011086991062424
2	12.792919679632023	5.517136002840264
3	6.124529475816093	3.9619410092849083
4	3.529034245659188	3.043896527073729
5	2.282539010325466	2.460946849334913
6	1.492235931617251	1.9306465113273257
7	1.1076996139328668	1.6719915005266206
8	0.8272322721320222	1.4270246560079123
9	0.6448807294558031	1.2515146273117062
>10	4.060149667668883	18.291034337623852
>50	1.4857338091593029	23.64083189654725
>100	0.664114946165201	18.760431728252474
>500	0.008570900322625627	1.2358615495596668
>1k	0.002597242522007766	1.0797757137585704
>5k	0.0010388970088031065	1.715880099488334
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	9253	0.5102581013744378	No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	9211	0.5079420049454174	No Hit
CTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGCT	6743	0.3718437671639289	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5431	0.2994933263335752	No Hit
TCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	4025	0.22195924111446147	No Hit
ACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	2703	0.14905734875338866	No Hit
CGCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTG	2458	0.13554678625076927	No Hit
ACCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTG	2008	0.11073146736840712	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.654354592157477E-4	0.0	0.0	0.38893876461622284	0.0
2	1.654354592157477E-4	0.0	0.0	1.8550829493392509	0.0
3	1.654354592157477E-4	0.0	0.0	2.6229240607126076	0.0
4	1.654354592157477E-4	0.0	0.0	3.472104272867041	0.0
5	1.654354592157477E-4	0.0	0.0	5.653977399310465	0.0
6	1.654354592157477E-4	0.0	0.0	6.778883376824477	0.0
7	2.2058061228766358E-4	0.0	0.0	8.053618735234885	0.0
8	2.2058061228766358E-4	0.0	0.0	10.160218727735144	0.0
9	2.2058061228766358E-4	0.0	0.0	10.926626065128632	0.0
10	2.2058061228766358E-4	0.0	0.0	12.46528612614123	0.0
11	2.2058061228766358E-4	0.0	0.0	14.746199947501815	0.0
12	2.2058061228766358E-4	0.0	0.0	16.564501079742097	0.0
13	2.2058061228766358E-4	0.0	0.0	17.26914584569504	0.0
14	2.2058061228766358E-4	0.0	0.0	17.535166064113962	0.0
15	2.2058061228766358E-4	0.0	0.0	18.044983004263823	0.0
16	2.2058061228766358E-4	0.0	0.0	19.13172853585207	0.0
17	2.2058061228766358E-4	0.0	0.0	20.47754599657218	0.0
18	2.2058061228766358E-4	0.0	0.0	22.012456187175886	0.0
19	2.7572576535957947E-4	0.0	0.0	22.895771249081832	0.0
20	2.7572576535957947E-4	0.0	0.0	23.72813218954933	0.0
21	2.7572576535957947E-4	0.0	0.0	24.7496410050535	0.0
22	2.7572576535957947E-4	0.0	0.0	25.80975142770801	0.0
23	2.7572576535957947E-4	0.0	0.0	26.852105111073367	0.0
24	2.7572576535957947E-4	0.0	0.0	27.662518280618244	0.0
25	2.7572576535957947E-4	0.0	0.0	28.382052237900602	0.0
26	2.7572576535957947E-4	0.0	0.0	29.03270989899614	0.0
27	3.308709184314954E-4	0.0	0.0	29.651107645544602	0.0
28	3.860160715034113E-4	0.0	0.0	30.282960809442613	0.0
29	3.860160715034113E-4	0.0	0.0	30.939243276151487	0.0
30	3.860160715034113E-4	0.0	0.0	31.720264079109032	0.0
31	3.860160715034113E-4	0.0	0.0	32.41525844327439	0.0
32	3.860160715034113E-4	0.0	0.0	33.07247837758548	0.0
33	3.860160715034113E-4	0.0	0.0	33.687126253725054	0.0
34	3.860160715034113E-4	0.0	0.0	34.27431184363481	0.0
35	4.4116122457532717E-4	0.0	0.0	34.92838850422081	0.0
36	4.963063776472431E-4	0.0	0.0	35.56647307041595	0.0
37	4.963063776472431E-4	0.0	0.0	36.20389589477423	0.0
38	6.065966837910749E-4	0.0	0.0	36.822348786475764	0.0
39	6.065966837910749E-4	0.0	0.0	37.44195972639181	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGCA	25	3.8917E-5	45.000004	40
CGTTCCA	25	3.8917E-5	45.000004	37
TATTCGT	25	3.8917E-5	45.000004	29
AACGTAT	20	7.0343673E-4	45.0	36
CGAGTTA	20	7.0343673E-4	45.0	18
CGTAAAT	20	7.0343673E-4	45.0	16
CGCGTTG	20	7.0343673E-4	45.0	13
ATCGATT	20	7.0343673E-4	45.0	22
AGTCCGT	30	2.166198E-6	44.999996	15
ATATGCG	60	0.0	44.999996	1
GTCAACG	30	2.166198E-6	44.999996	1
CGCCGAT	30	2.166198E-6	44.999996	27
GCGATAT	80	0.0	42.1875	9
TCGATTG	65	0.0	41.538464	1
CGTTTTT	3285	0.0	41.36986	1
ACGGGTT	55	6.184564E-11	40.909092	5
CACGTAA	100	0.0	40.500004	27
TATTGCG	140	0.0	40.178574	1
GGTCGAC	40	3.4596997E-7	39.375	8
TATTACG	40	3.4596997E-7	39.375	1
>>END_MODULE