##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934738.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	976784
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13640170191158	33.0	31.0	34.0	30.0	34.0
2	32.32043931923537	34.0	31.0	34.0	30.0	34.0
3	32.32302228537732	34.0	31.0	34.0	30.0	34.0
4	35.87758091860636	37.0	35.0	37.0	35.0	37.0
5	35.7722208799489	37.0	35.0	37.0	35.0	37.0
6	35.70754537338859	37.0	35.0	37.0	33.0	37.0
7	36.02052040164458	37.0	35.0	37.0	35.0	37.0
8	35.845862544841026	37.0	35.0	37.0	35.0	37.0
9	37.568101033595966	39.0	38.0	39.0	35.0	39.0
10	37.32598199806713	39.0	37.0	39.0	34.0	39.0
11	37.20595239070255	39.0	37.0	39.0	34.0	39.0
12	36.96051020491736	39.0	35.0	39.0	33.0	39.0
13	36.76920997886943	39.0	35.0	39.0	33.0	39.0
14	37.828584415797145	40.0	36.0	41.0	33.0	41.0
15	37.9299353797769	40.0	36.0	41.0	33.0	41.0
16	38.075619584268374	40.0	36.0	41.0	34.0	41.0
17	38.07098498746908	40.0	36.0	41.0	34.0	41.0
18	38.08136189781978	40.0	36.0	41.0	34.0	41.0
19	38.0227972612164	40.0	36.0	41.0	34.0	41.0
20	37.9177975888221	40.0	36.0	41.0	34.0	41.0
21	37.83039443725532	40.0	35.0	41.0	34.0	41.0
22	37.76484360923193	39.0	35.0	41.0	33.0	41.0
23	37.56655923929958	39.0	35.0	41.0	33.0	41.0
24	37.33348314468706	39.0	35.0	41.0	33.0	41.0
25	37.256268530197055	39.0	35.0	41.0	33.0	41.0
26	37.42019013415453	39.0	35.0	41.0	33.0	41.0
27	37.44290651771528	39.0	35.0	41.0	33.0	41.0
28	37.433266720175595	39.0	35.0	41.0	33.0	41.0
29	37.446599248144935	39.0	35.0	41.0	33.0	41.0
30	37.25662377762126	39.0	35.0	41.0	33.0	41.0
31	37.16735941620665	39.0	35.0	41.0	33.0	41.0
32	37.08368585071008	39.0	35.0	41.0	32.0	41.0
33	36.868204229389505	39.0	35.0	41.0	32.0	41.0
34	36.85865861848679	39.0	35.0	41.0	32.0	41.0
35	36.793530606561944	39.0	35.0	41.0	32.0	41.0
36	36.612407656145066	39.0	35.0	41.0	31.0	41.0
37	36.54875489361005	39.0	35.0	41.0	31.0	41.0
38	36.47440785270848	39.0	35.0	41.0	31.0	41.0
39	36.47368097757539	39.0	35.0	41.0	31.0	41.0
40	36.393346942619864	39.0	35.0	40.0	31.0	41.0
41	36.33406976363249	39.0	35.0	40.0	31.0	41.0
42	36.22081340398696	38.0	35.0	40.0	31.0	41.0
43	36.14086021065046	38.0	35.0	40.0	30.0	41.0
44	36.07704569280414	38.0	35.0	40.0	30.0	41.0
45	35.982547830431294	38.0	35.0	40.0	30.0	41.0
46	35.89885071827548	38.0	35.0	40.0	30.0	41.0
47	35.788748587200445	38.0	35.0	40.0	30.0	41.0
48	35.763780938262705	38.0	35.0	40.0	30.0	41.0
49	35.74629498435683	38.0	35.0	40.0	30.0	41.0
50	35.615578265000245	37.0	35.0	40.0	29.0	41.0
51	34.62806004193353	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	12.0
10	17.0
11	18.0
12	13.0
13	19.0
14	19.0
15	32.0
16	63.0
17	117.0
18	273.0
19	492.0
20	801.0
21	1343.0
22	1983.0
23	2844.0
24	4079.0
25	5877.0
26	8024.0
27	9849.0
28	11195.0
29	12834.0
30	15234.0
31	19036.0
32	25128.0
33	35966.0
34	70259.0
35	101096.0
36	78048.0
37	119143.0
38	204678.0
39	248159.0
40	131.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.497354584022666	22.2085947353765	25.64978541827057	13.64426526233026
2	31.25962341725499	25.01238759029632	24.93376222378745	18.79422676866124
3	30.582605775688382	23.629891562515358	29.004774852986948	16.782727808809316
4	28.301036872020834	26.146517551475046	27.27552867368835	18.27691690281577
5	24.66471604776491	29.623949614244292	26.401230978394402	19.31010335959639
6	25.73424626120002	33.570472079804745	25.107597995053155	15.587683663942078
7	77.07364166489214	6.857913315533424	10.542248849285	5.526196170289439
8	76.66300840308604	5.288784419073203	10.880911235237269	7.167295942603483
9	71.67449507772446	6.005524251011483	13.683066061688152	8.636914609575914
10	42.14278694163704	21.620133007911676	20.199552818227982	16.037527232223294
11	32.40665285262658	22.45030631132369	24.158872381201984	20.984168454847747
12	27.91528116758669	19.204348146570787	32.316049402938624	20.564321282903897
13	23.15476092974496	25.115481007059902	33.27347704303101	18.45628102016413
14	18.36608707759341	28.538243869678453	30.89372880800668	22.201940244721452
15	16.26449655195007	23.461891267670232	39.83992366787335	20.433688512506347
16	19.13329866173074	22.726314108339203	34.9042367606349	23.236150469295154
17	19.63218070730069	22.70839817196023	29.706362921587576	27.953058199151503
18	21.80041851627381	22.247907418630934	32.13044030205245	23.8212337630428
19	23.599383282281448	24.96867270553162	27.305627446805026	24.126316565381906
20	24.286126717882357	24.88114055922292	29.102237546888567	21.73049517600616
21	22.358269586725417	26.07413716850399	30.681194614162393	20.8863986306082
22	21.346684630378874	24.30783059509574	26.94843486379793	27.39704991072745
23	19.762096840243085	27.02787924454127	27.800721551540562	25.409302363675078
24	22.179519730052906	22.371169060918277	30.91225900506151	24.537052203967306
25	20.724336188962962	24.338850759226197	29.865046929515636	25.07176612229521
26	21.22311585775361	26.517326246130157	28.419486805680684	23.84007109043555
27	22.267768513816772	27.084698357057448	28.084612360562826	22.562920768562957
28	20.720957755245788	24.387479729397697	31.64630051270291	23.245262002653604
29	24.71866861046045	22.511937132467363	29.14984274926698	23.619551507805205
30	23.58945273468853	27.958279414896232	25.557748693672295	22.894519156742945
31	25.794443807433375	27.837679568870904	24.082704057396516	22.285172566299202
32	26.670481907975564	27.408106602892758	22.98768202591361	22.933729463218068
33	23.637672197742795	29.235532113548135	23.486461694704253	23.640333994004816
34	23.125276417304132	24.949118740683712	28.602843617422067	23.32276122459008
35	22.982665563727497	24.52886206162263	26.24172795623188	26.246744418417993
36	24.855546364395813	28.493402840341364	25.691145637111173	20.95990515815165
37	23.904773214958475	27.094526527871054	28.895436452685548	20.10526380448492
38	22.813231993972057	28.545205490671428	24.24701878818654	24.394543727169978
39	23.90272567937231	26.661063244279188	27.369817687431407	22.0663933889171
40	23.997219446673984	23.405891169388525	29.035999770676014	23.56088961326148
41	19.91617389310226	25.64139052236728	26.037793411849496	28.404642172680962
42	23.211477665481826	24.396591262756147	26.611103375976676	25.78082769578535
43	24.756752772363182	24.84018984749955	26.991433111107472	23.411624269029797
44	24.29319071565464	24.576160133663123	26.69259529230618	24.43805385837606
45	23.957804386640237	23.978279742501922	25.821778407508724	26.242137463349113
46	24.144744385657425	25.580783469016694	27.116639912201673	23.15783223312421
47	20.36847450408688	24.450339071893072	31.012281118445838	24.168905305574214
48	20.40164458058281	22.83176219102688	29.7856025487723	26.98099067961801
49	22.30411227047126	21.285565693131748	32.33672951235892	24.07359252403807
50	21.683913741420827	21.151247358679093	31.163286867925766	26.001552031974317
51	20.41915100984455	22.649224393519958	26.41914691477338	30.51247768186211
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	359.0
1	597.0
2	835.0
3	3973.5
4	7112.0
5	4815.5
6	2519.0
7	2491.5
8	2464.0
9	2590.5
10	2717.0
11	2769.0
12	2821.0
13	2752.5
14	2684.0
15	2504.5
16	2325.0
17	2306.0
18	2287.0
19	2345.0
20	2403.0
21	2321.0
22	2239.0
23	2355.5
24	2472.0
25	3027.5
26	4398.0
27	5213.0
28	5338.0
29	5463.0
30	7145.0
31	8827.0
32	10294.0
33	11761.0
34	14583.5
35	17406.0
36	18176.0
37	18946.0
38	21404.0
39	23862.0
40	28764.0
41	33666.0
42	43468.0
43	53270.0
44	62083.0
45	70896.0
46	83333.0
47	95770.0
48	113609.0
49	131448.0
50	126691.5
51	121935.0
52	101119.0
53	80303.0
54	68429.0
55	56555.0
56	50692.5
57	44830.0
58	41863.5
59	38897.0
60	35391.5
61	31886.0
62	30850.5
63	29815.0
64	25704.5
65	21594.0
66	17275.0
67	12956.0
68	11579.5
69	10203.0
70	8170.5
71	6138.0
72	4776.5
73	3415.0
74	2776.0
75	1895.5
76	1654.0
77	1130.5
78	607.0
79	507.0
80	407.0
81	231.0
82	55.0
83	39.5
84	24.0
85	17.0
86	10.0
87	5.5
88	1.0
89	5.0
90	9.0
91	6.5
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	976784.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.994157137304626
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.67976382615812	15.332449672970409
2	12.212246872332368	5.616206471639348
3	5.886495779838151	4.06065026849037
4	3.3679320664242276	3.0977103665250314
5	2.167153297412482	2.4915931730665237
6	1.5214079972853773	2.099009673571914
7	1.0648111799975797	1.7139104916096177
8	0.807721784181622	1.4858305302957018
9	0.608797771834135	1.2598912467355525
>10	3.767964271464745	17.784374850938182
>50	1.2904621114955848	21.774345809883993
>100	0.6144629891346718	18.84380554994782
>500	0.005390026220479577	0.8359692961938826
>1k	0.004491688517066314	2.3482330020094038
>5k	8.983377034132629E-4	1.2560195961222886
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC	6378	0.6529590984291307	No Hit
CCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC	5783	0.5920449147406183	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	4719	0.4831160215564546	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4073	0.4169806221232125	No Hit
CTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGCT	3614	0.3699896804206457	No Hit
TCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC	2499	0.2558395714917525	No Hit
ACTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC	1839	0.18827089714819242	No Hit
CGCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTG	1797	0.18397107241723862	No Hit
GCCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTG	1079	0.11046454487378991	No Hit
ACCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTG	1062	0.1087241396255467	No Hit
GGCAAGGTGGGGTGAGGCCCGGAGTTGGGGAAGCTAGGAGGCTTAAAGCCT	1047	0.10718848793592033	No Hit
AGCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTG	1007	0.10309341676358334	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0237677930842438E-4	0.0	0.0	0.3901579059444053	0.0
2	1.0237677930842438E-4	0.0	0.0	2.171411489131681	0.0
3	1.0237677930842438E-4	0.0	0.0	3.059325296073646	0.0
4	1.0237677930842438E-4	0.0	0.0	3.9919777555733917	0.0
5	1.0237677930842438E-4	0.0	0.0	6.4189216858589	0.0
6	1.0237677930842438E-4	0.0	0.0	7.692898327572933	0.0
7	1.0237677930842438E-4	0.0	0.0	9.114502285049713	0.0
8	1.0237677930842438E-4	0.0	0.0	11.32010761847041	0.0
9	1.0237677930842438E-4	0.0	0.0	12.098785401890284	0.0
10	1.0237677930842438E-4	0.0	0.0	13.80602057363757	0.0
11	1.0237677930842438E-4	0.0	0.0	16.303297351307965	0.0
12	1.0237677930842438E-4	0.0	0.0	18.216002719127257	0.0
13	1.0237677930842438E-4	0.0	0.0	18.97809546429917	0.0
14	1.0237677930842438E-4	0.0	0.0	19.238849121197727	0.0
15	1.0237677930842438E-4	0.0	0.0	19.777760487477273	0.0
16	1.0237677930842438E-4	0.0	0.0	20.964000229323986	0.0
17	1.0237677930842438E-4	0.0	0.0	22.36185277400121	0.0
18	1.0237677930842438E-4	0.0	0.0	24.005307212239348	0.0
19	1.0237677930842438E-4	0.0	0.0	24.89782797425019	0.0
20	1.0237677930842438E-4	0.0	0.0	25.739160346606823	0.0
21	1.0237677930842438E-4	0.0	0.0	26.787908073842324	0.0
22	1.0237677930842438E-4	0.0	0.0	27.75178545103114	0.0
23	1.0237677930842438E-4	0.0	0.0	28.78486953103245	0.0
24	1.0237677930842438E-4	0.0	0.0	29.55208111516978	0.0
25	1.0237677930842438E-4	0.0	0.0	30.29676980785926	0.0
26	1.0237677930842438E-4	0.0	0.0	30.932120100247342	0.0
27	1.0237677930842438E-4	0.0	0.0	31.548121181346133	0.0
28	1.0237677930842438E-4	0.0	0.0	32.193913905223674	0.0
29	1.0237677930842438E-4	0.0	0.0	32.79896067093646	0.0
30	1.0237677930842438E-4	0.0	0.0	33.50075349309571	0.0
31	1.0237677930842438E-4	0.0	0.0	34.173676063489985	0.0
32	1.0237677930842438E-4	0.0	0.0	34.779439466657934	0.0
33	2.0475355861684875E-4	0.0	0.0	35.414380251928776	0.0
34	2.0475355861684875E-4	0.0	0.0	35.9991564153385	0.0
35	2.0475355861684875E-4	0.0	0.0	36.67218136251208	0.0
36	2.0475355861684875E-4	0.0	0.0	37.294939311045226	0.0
37	2.0475355861684875E-4	0.0	0.0	37.85381414928992	0.0
38	2.0475355861684875E-4	0.0	0.0	38.40951530737604	0.0
39	2.0475355861684875E-4	0.0	0.0	38.96644498681387	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAG	35	1.2116834E-7	45.000004	1
TCGGTAT	35	1.2116834E-7	45.000004	5
TTGATCG	20	7.032766E-4	45.0	1
CTAGAAA	20	7.032766E-4	45.0	9
CTATGCG	40	6.8139343E-9	45.0	1
TCGTTCA	40	6.8139343E-9	45.0	23
AGTCCAT	20	7.032766E-4	45.0	11
ACGCCTA	20	7.032766E-4	45.0	33
CGTATTT	25	3.890375E-5	45.0	22
CTCGTAG	20	7.032766E-4	45.0	1
GATCGAC	25	3.890375E-5	45.0	3
CGCGGGT	80	0.0	45.0	4
ACGTTGC	20	7.032766E-4	45.0	21
CTATCCG	20	7.032766E-4	45.0	34
TAGACGT	20	7.032766E-4	45.0	25
ACGCAAT	20	7.032766E-4	45.0	33
CGTTGAG	40	6.8139343E-9	45.0	17
TAGCGGA	20	7.032766E-4	45.0	43
CCGGATC	20	7.032766E-4	45.0	13
ATAGCGT	20	7.032766E-4	45.0	14
>>END_MODULE