##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934737.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	884885
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.96627923402476	33.0	31.0	34.0	30.0	34.0
2	32.157778694406616	33.0	31.0	34.0	30.0	34.0
3	32.155467659639385	34.0	31.0	34.0	30.0	34.0
4	35.77100979223289	37.0	35.0	37.0	35.0	37.0
5	35.64568955287975	37.0	35.0	37.0	33.0	37.0
6	35.589769292054896	37.0	35.0	37.0	33.0	37.0
7	35.975178695536705	37.0	35.0	37.0	35.0	37.0
8	35.83533001463467	37.0	35.0	37.0	35.0	37.0
9	37.62602485068681	39.0	38.0	39.0	35.0	39.0
10	37.18830356487001	39.0	37.0	39.0	34.0	39.0
11	37.08294863174311	39.0	37.0	39.0	33.0	39.0
12	36.972034784181	39.0	35.0	39.0	33.0	39.0
13	36.81441204224278	39.0	35.0	39.0	33.0	39.0
14	37.8016420212796	40.0	36.0	41.0	33.0	41.0
15	37.898690790328686	40.0	36.0	41.0	33.0	41.0
16	38.04387010741509	40.0	37.0	41.0	33.0	41.0
17	38.06010272521288	40.0	37.0	41.0	34.0	41.0
18	38.04796329466541	39.0	37.0	41.0	34.0	41.0
19	38.02716398176034	40.0	37.0	41.0	34.0	41.0
20	37.93042937782876	39.0	36.0	41.0	34.0	41.0
21	37.88172474389327	39.0	36.0	41.0	34.0	41.0
22	37.82543042316233	39.0	36.0	41.0	33.0	41.0
23	37.649487786548534	39.0	35.0	41.0	33.0	41.0
24	37.382436135769055	39.0	35.0	41.0	33.0	41.0
25	37.347071088333514	39.0	35.0	41.0	33.0	41.0
26	37.54769489820711	39.0	35.0	41.0	33.0	41.0
27	37.63540347050747	39.0	35.0	41.0	33.0	41.0
28	37.65382168304356	39.0	36.0	41.0	33.0	41.0
29	37.67836159500952	39.0	36.0	41.0	33.0	41.0
30	37.4470162789515	39.0	36.0	41.0	33.0	41.0
31	37.334035496137915	39.0	35.0	41.0	33.0	41.0
32	37.24608169423146	39.0	35.0	41.0	33.0	41.0
33	37.02313520966001	39.0	35.0	41.0	32.0	41.0
34	36.867243766139104	39.0	35.0	41.0	31.0	41.0
35	36.832071964153535	39.0	35.0	41.0	31.0	41.0
36	36.68607107138216	39.0	35.0	40.0	31.0	41.0
37	36.472091853743706	39.0	35.0	40.0	30.0	41.0
38	36.45059075473084	39.0	35.0	40.0	30.0	41.0
39	36.45637907750724	39.0	35.0	40.0	31.0	41.0
40	36.337767054476004	39.0	35.0	40.0	30.0	41.0
41	36.40736592890602	39.0	35.0	40.0	30.0	41.0
42	36.30883222113608	39.0	35.0	40.0	30.0	41.0
43	36.17130361572408	38.0	35.0	40.0	30.0	41.0
44	36.12226673522548	38.0	35.0	40.0	30.0	41.0
45	36.14155624742198	38.0	35.0	40.0	30.0	41.0
46	36.151219650011015	38.0	35.0	40.0	30.0	41.0
47	36.00882600563915	38.0	35.0	40.0	30.0	41.0
48	36.001486068811204	38.0	35.0	40.0	30.0	41.0
49	35.984669194302086	38.0	35.0	40.0	29.0	41.0
50	35.854255637738234	38.0	35.0	40.0	29.0	41.0
51	34.93201150431977	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	5.0
10	13.0
11	11.0
12	8.0
13	11.0
14	13.0
15	15.0
16	45.0
17	77.0
18	164.0
19	326.0
20	572.0
21	969.0
22	1525.0
23	2422.0
24	3576.0
25	5339.0
26	7165.0
27	8515.0
28	9160.0
29	10807.0
30	13229.0
31	17660.0
32	23449.0
33	33189.0
34	59434.0
35	84486.0
36	74592.0
37	115884.0
38	205152.0
39	206959.0
40	110.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.56149104120875	23.1593935935178	27.135729501573653	12.143385863699804
2	31.38091390406663	24.517875204122568	26.383202337026844	17.718008554783953
3	28.786452476875525	24.69213513620414	30.175559535984902	16.345852850935433
4	26.891629985817367	24.821869508467202	30.09351497652237	18.19298552919306
5	23.643072263627477	28.143205049243686	28.98625245088345	19.227470236245388
6	24.28937093520627	31.628403690875086	27.481650157930126	16.60057521598852
7	75.99778502291258	4.921882504506235	12.640738627053233	6.439593845527949
8	75.54687897297389	3.8238867197432436	12.231871938161456	8.397362369121412
9	71.52669555930997	4.704340111991954	14.750843329924226	9.01812099877385
10	36.46033100346372	23.057798470987755	23.11418997948886	17.367680546059656
11	25.820191324296378	25.940884973753654	27.274165569537285	20.964758132412687
12	24.978161003972268	22.33273250196353	33.37654045440933	19.31256603965487
13	21.40990072156269	26.913779756691547	35.648474095503936	16.027845426241825
14	16.995541793566396	30.509614243658778	31.55856410720037	20.93627985557445
15	14.714680438701075	26.21493188380411	40.65228815043763	18.418099527057187
16	15.685767077077811	26.414505839741885	34.59658599705047	23.303141086129838
17	16.565881442221304	25.68051215694695	30.19296292738605	27.5606434734457
18	17.664216254089517	25.29413426603457	35.62982760471699	21.411821875158918
19	18.79362855060262	26.969832238087438	31.484204162122765	22.752335049187185
20	19.234815823525093	29.019251089124577	31.431089915638754	20.314843171711576
21	18.605694525277297	28.35464495386406	32.74391587607429	20.29574464478435
22	16.20312243963905	26.538815778321478	29.626787661673546	27.631274120365923
23	16.146278894997653	27.27100131655526	30.688055510038026	25.894664278409056
24	19.678489295219155	24.360679636336926	32.32668651858716	23.63414454985676
25	15.941732541516696	27.87932895234974	29.66792295043989	26.511015555693678
26	15.658644908660445	28.215869858795212	33.219909931799045	22.905575300745294
27	18.406459596444734	29.389694706091753	30.299191420354056	21.904654277109454
28	14.227950524644445	26.02948405725038	32.561406284432444	27.18115913367274
29	17.8175695146827	24.502392966317657	32.905744814297904	24.774292704701743
30	19.338219090616295	31.47889273747436	27.742475010877122	21.440413161032225
31	19.236058922910885	31.695531057708067	24.573362640343095	24.495047379037953
32	23.38507263655729	29.35974731179758	25.30193188945456	21.953248162190565
33	22.675827932443198	30.970352079648766	23.47028144900185	22.883538538906187
34	18.443413550913395	27.437011589076548	28.740231781530934	25.37934307847912
35	22.282556490391407	26.688213722687127	27.637150590189684	23.39207919673178
36	24.048096645326794	29.41557377512332	26.334834470015878	20.201495109534008
37	21.37486792069026	29.257135107951882	28.02963096899597	21.338366002361887
38	23.26991642981856	28.24344406335286	24.209021511269828	24.277617995558746
39	24.520022375788944	27.87718178068336	26.869480214943188	20.733315628584506
40	23.21544607491369	26.812410652231645	29.25125863812812	20.72088463472655
41	22.687354854020576	26.83241325143945	25.730801177554145	24.749430716985824
42	25.18417647490917	23.136565768433186	28.542578979189386	23.13667877746826
43	25.76210468026919	22.946258553371344	28.05268481215073	23.23895195420874
44	21.236318843691553	24.90493114924538	26.82133836600236	27.037411641060704
45	21.951553026664484	24.7920351232081	26.096950451188572	27.159461398938845
46	24.74219813874119	25.834882498855784	27.71908214061714	21.703837221785882
47	19.6966837498658	25.382168304355936	31.748984331297287	23.17216361448098
48	22.22752109031117	22.484503636065703	29.543273984755082	25.744701288868043
49	23.78478559360821	19.739062138017935	32.86924289596953	23.606909372404324
50	21.59670465653729	20.5219887329992	31.108788147612405	26.772518462851103
51	20.59578363290145	20.51758138063138	26.235386519152204	32.651248467314964
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	300.0
1	425.5
2	551.0
3	3598.5
4	6646.0
5	4227.0
6	1808.0
7	1944.0
8	2080.0
9	2193.5
10	2307.0
11	2559.5
12	2812.0
13	2994.0
14	3176.0
15	3069.0
16	2962.0
17	2777.5
18	2593.0
19	2718.5
20	2844.0
21	2741.0
22	2638.0
23	2696.5
24	2755.0
25	3235.0
26	4365.0
27	5015.0
28	5836.5
29	6658.0
30	8048.0
31	9438.0
32	11456.5
33	13475.0
34	15815.0
35	18155.0
36	20752.5
37	23350.0
38	25843.0
39	28336.0
40	33162.5
41	37989.0
42	47211.0
43	56433.0
44	65726.0
45	75019.0
46	95796.0
47	116573.0
48	130518.5
49	144464.0
50	136423.5
51	128383.0
52	100953.5
53	73524.0
54	57181.5
55	40839.0
56	32486.5
57	24134.0
58	19976.5
59	15819.0
60	13694.5
61	11570.0
62	9374.0
63	7178.0
64	6174.0
65	5170.0
66	3837.0
67	2504.0
68	2050.0
69	1596.0
70	1191.0
71	786.0
72	667.0
73	548.0
74	442.5
75	255.0
76	173.0
77	171.5
78	170.0
79	104.0
80	38.0
81	24.0
82	10.0
83	11.0
84	12.0
85	7.0
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	884885.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.343307202468697
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.03248840467477	17.131708201853304
2	12.898633050221303	6.795853058677912
3	6.280010201130453	4.963087138890502
4	3.713944505492132	3.913503241643996
5	2.327272903995601	3.065403252696877
6	1.6302133008667448	2.5767125874169903
7	1.1021176046005212	2.0323395841868317
8	0.8525882452712	1.7967995249914361
9	0.6774702392839621	1.6062125970588652
>10	4.333691395596707	22.99327399263745
>50	0.8759214422958067	16.5168265350643
>100	0.26134617934308846	10.27365036431154
>500	0.007801378487853386	1.357192348253776
>1k	0.005634328907894112	3.3045527160350834
>5k	8.668198319837096E-4	1.672884856281175
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC	8212	0.9280301960141714	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC	6440	0.72777818586596	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4882	0.5517101092232324	No Hit
CTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGCT	4725	0.5339676907168728	Illumina Single End Adapter 1 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC	4089	0.46209394441085566	Illumina Single End Adapter 1 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC	2598	0.2935974731179758	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTG	1948	0.22014160032094565	No Hit
ACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTG	1546	0.17471196822185933	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGC	1531	0.17301683269577403	No Hit
GCCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTG	1418	0.16024681173259803	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTC	1386	0.15663052261028268	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCG	1309	0.14792882690971143	No Hit
AGCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTG	1301	0.14702475462913261	No Hit
GGCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTG	1150	0.12996039033320714	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCC	1060	0.11978957717669526	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.565610220537132	0.0
2	0.0	0.0	0.0	3.1277510636975427	0.0
3	0.0	0.0	0.0	4.4316493103623635	0.0
4	0.0	0.0	0.0	5.870254326833431	0.0
5	0.0	0.0	0.0	9.564406674313611	0.0
6	0.0	0.0	0.0	11.42555247292021	0.0
7	0.0	0.0	0.0	13.525599371669765	0.0
8	0.0	0.0	0.0	16.61165010142561	0.0
9	0.0	0.0	0.0	17.628505399006652	0.0
10	0.0	0.0	0.0	20.273368855840026	0.0
11	0.0	0.0	0.0	24.090136006373708	0.0
12	0.0	0.0	0.0	26.86349073608435	0.0
13	0.0	0.0	0.0	27.89469818111958	0.0
14	0.0	0.0	0.0	28.27101826791052	0.0
15	0.0	0.0	0.0	28.979245890708963	0.0
16	0.0	0.0	0.0	30.68477824802093	0.0
17	0.0	0.0	0.0	32.80200252010148	0.0
18	0.0	0.0	0.0	35.07371014312594	0.0
19	0.0	0.0	0.0	36.33726416426993	0.0
20	0.0	0.0	0.0	37.4692756685897	0.0
21	0.0	0.0	0.0	38.96122094961492	0.0
22	0.0	0.0	0.0	40.40366827327845	0.0
23	0.0	0.0	0.0	41.8267910519446	0.0
24	0.0	0.0	0.0	42.91077371635862	0.0
25	0.0	0.0	0.0	43.84784463517858	0.0
26	0.0	0.0	0.0	44.693943280765296	0.0
27	0.0	0.0	0.0	45.437429722506316	0.0
28	0.0	0.0	0.0	46.212671703102664	0.0
29	0.0	0.0	0.0	47.002943885363635	0.0
30	0.0	0.0	0.0	47.92532362962419	0.0
31	0.0	0.0	0.0	48.76023438073874	0.0
32	0.0	0.0	0.0	49.50213869598874	0.0
33	1.1300903507235404E-4	0.0	0.0	50.18222706905417	0.0
34	1.1300903507235404E-4	0.0	0.0	50.915994733778966	0.0
35	1.1300903507235404E-4	0.0	0.0	51.65869011227448	0.0
36	1.1300903507235404E-4	0.0	0.0	52.305779847098776	0.0
37	1.1300903507235404E-4	0.0	0.0	52.92687750385643	0.0
38	1.1300903507235404E-4	0.0	0.0	53.58639823253869	0.0
39	1.1300903507235404E-4	0.0	0.0	54.24716206060675	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCCGT	20	7.0324045E-4	45.000004	10
CTTCGCG	30	2.164934E-6	45.000004	1
TCGCATA	20	7.0324045E-4	45.000004	35
TCGTTAG	30	2.164934E-6	45.000004	1
GCAAGTC	20	7.0324045E-4	45.000004	14
TCGCACA	20	7.0324045E-4	45.000004	29
ACCGGGT	30	2.164934E-6	45.000004	4
AGATCGA	20	7.0324045E-4	45.000004	14
CGAGTTA	30	2.164934E-6	45.000004	36
GGTAACG	45	3.8380676E-10	45.000004	1
GATCGAT	20	7.0324045E-4	45.000004	15
GATCGAA	20	7.0324045E-4	45.000004	9
GCGCGAG	45	3.8380676E-10	45.000004	1
CCTACGG	20	7.0324045E-4	45.000004	2
CGTCTGG	35	1.2115015E-7	45.000004	2
TTCGTAG	30	2.164934E-6	45.000004	1
ATAGACG	30	2.164934E-6	45.000004	1
TAACGTG	35	1.2115015E-7	45.000004	1
CCTCGTA	20	7.0324045E-4	45.000004	17
AGGCGAT	35	1.2115015E-7	45.000004	6
>>END_MODULE