##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934736.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1666782
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17723553530096	33.0	31.0	34.0	30.0	34.0
2	32.35600216465021	34.0	31.0	34.0	30.0	34.0
3	32.35336714699343	34.0	31.0	34.0	30.0	34.0
4	35.916593771711	37.0	35.0	37.0	35.0	37.0
5	35.80967037081034	37.0	35.0	37.0	35.0	37.0
6	35.73905765721012	37.0	35.0	37.0	35.0	37.0
7	36.08968539377075	37.0	35.0	37.0	35.0	37.0
8	35.94724265080856	37.0	35.0	37.0	35.0	37.0
9	37.64932966638709	39.0	38.0	39.0	35.0	39.0
10	37.37753707443445	39.0	37.0	39.0	34.0	39.0
11	37.228266203978684	39.0	37.0	39.0	34.0	39.0
12	37.154673496594036	39.0	37.0	39.0	34.0	39.0
13	37.095401198237084	39.0	37.0	39.0	33.0	39.0
14	38.20430446213122	40.0	37.0	41.0	33.0	41.0
15	38.28349898187045	40.0	37.0	41.0	34.0	41.0
16	38.37737208585166	40.0	37.0	41.0	34.0	41.0
17	38.372709808481254	40.0	37.0	41.0	34.0	41.0
18	38.364694963108555	40.0	37.0	41.0	34.0	41.0
19	38.333137146909436	40.0	37.0	41.0	34.0	41.0
20	38.28552384175015	40.0	37.0	41.0	34.0	41.0
21	38.21940901689603	40.0	37.0	41.0	34.0	41.0
22	38.15377475878669	40.0	37.0	41.0	34.0	41.0
23	37.98591657457304	40.0	36.0	41.0	34.0	41.0
24	37.74576339317319	39.0	36.0	41.0	33.0	41.0
25	37.685690150241605	39.0	36.0	41.0	33.0	41.0
26	37.88963163749069	40.0	36.0	41.0	34.0	41.0
27	37.96741625479517	40.0	36.0	41.0	34.0	41.0
28	37.93387677572712	40.0	36.0	41.0	34.0	41.0
29	37.97909444666429	40.0	36.0	41.0	34.0	41.0
30	37.861507983647535	40.0	36.0	41.0	34.0	41.0
31	37.73693740393165	40.0	36.0	41.0	33.0	41.0
32	37.6207482442215	40.0	36.0	41.0	33.0	41.0
33	37.50049796554079	40.0	36.0	41.0	33.0	41.0
34	37.445353381546	40.0	36.0	41.0	33.0	41.0
35	37.369920001535895	40.0	36.0	41.0	33.0	41.0
36	37.2749219753993	40.0	35.0	41.0	33.0	41.0
37	37.172460465735774	39.0	35.0	41.0	32.0	41.0
38	37.07994986746917	39.0	35.0	41.0	32.0	41.0
39	37.12523593367339	39.0	35.0	41.0	32.0	41.0
40	37.07725905367349	39.0	35.0	41.0	32.0	41.0
41	36.99219634001327	39.0	35.0	41.0	32.0	41.0
42	36.87200005759601	39.0	35.0	41.0	31.0	41.0
43	36.8298175766237	39.0	35.0	41.0	31.0	41.0
44	36.79584612744798	39.0	35.0	41.0	31.0	41.0
45	36.784973679821356	39.0	35.0	41.0	31.0	41.0
46	36.72191984314686	39.0	35.0	41.0	31.0	41.0
47	36.575997940942486	39.0	35.0	41.0	31.0	41.0
48	36.52188588549672	39.0	35.0	41.0	31.0	41.0
49	36.50736029066788	39.0	35.0	40.0	31.0	41.0
50	36.41167711194385	39.0	35.0	40.0	31.0	41.0
51	35.47347703538915	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	14.0
10	22.0
11	24.0
12	16.0
13	21.0
14	32.0
15	30.0
16	61.0
17	143.0
18	280.0
19	496.0
20	835.0
21	1498.0
22	2342.0
23	3517.0
24	5287.0
25	7994.0
26	10998.0
27	13350.0
28	15008.0
29	17556.0
30	21850.0
31	27816.0
32	37394.0
33	53133.0
34	97434.0
35	139163.0
36	125346.0
37	195680.0
38	367519.0
39	521567.0
40	346.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.76722810781494	22.454946117728653	25.114322088911443	12.66350368554496
2	31.347470755023753	24.532782331462663	26.542583253238877	17.577163660274707
3	30.084198173486392	24.306897962660983	29.48735947472435	16.121544389128275
4	27.81209540299811	26.152910218612874	28.433472403709665	17.60152197467935
5	25.17803767979256	28.6019407457004	27.11050395312644	19.1095176213806
6	25.75291789808145	32.48625195136496	26.39655335850759	15.364276792045992
7	79.57399347965121	5.250596658711217	9.58985638193837	5.585553479699205
8	80.28062458077902	3.617569664179239	9.485763585159907	6.616042169881845
9	74.55192100706631	6.112916986144558	12.364844352770788	6.970317654018341
10	39.34611724868639	27.704342859474124	18.748582598084212	14.200957293755273
11	26.12297229031751	24.959052833543918	28.511527002331437	20.40644787380713
12	24.53512216954587	22.604035800722592	32.254607981127705	20.606234048603834
13	22.036295088379884	25.615287422110388	33.08747034705198	19.260947142457745
14	18.532237569160216	29.114965244405088	30.715654476710213	21.63714270972449
15	17.675376863921016	25.888328527665887	36.63706471512172	19.799229893291383
16	19.225969562906247	26.573481115106834	32.3727398064054	21.827809515581524
17	19.466072947752018	25.797434817510627	29.17184130858145	25.564650926155906
18	20.384669380878844	25.55529157382309	32.22197023965941	21.83806880563865
19	20.747584267168712	26.783106609022656	29.809297196633995	22.66001192717464
20	22.42032851326688	27.007671069162015	29.74672152687034	20.825278890700762
21	21.812450578419973	27.455240097385257	30.313742289033595	20.418567035161168
22	20.613493546246602	25.75945744554477	27.947326045037684	25.67972296317095
23	19.091458871046125	26.711171586926184	29.32117097496853	24.87619856705916
24	21.880845845467494	24.428329559594474	30.17449192515878	23.516332669779253
25	18.060130238987462	27.090525335646774	29.077227855832376	25.772116569533388
26	19.27546613774327	26.894818878533606	30.70959489603319	23.120120087689934
27	21.6555014393004	27.71448215783468	28.842104126394453	21.78791227647047
28	18.188881329412006	27.08062602067937	31.11774665193169	23.61274599797694
29	20.92799178296862	24.06667458611864	31.1091672456266	23.89616638528614
30	25.216854993634442	25.948684351042907	27.744600073674903	21.089860581647752
31	24.37583319234309	28.310840889810425	25.463497925943525	21.84982799190296
32	24.613836722498803	28.617299682861947	26.059856657919273	20.70900693671998
33	25.991881361809767	27.425122181545035	25.238573490714444	21.344422965930757
34	23.11706029942728	26.614938246273358	27.46729926289101	22.800702191408355
35	23.387701571051284	25.991461390871752	27.22815581161784	23.39268122645913
36	25.864330188350966	27.94696606994796	26.40369286445378	19.785010877247295
37	24.609996988208415	28.454410954761933	28.0199810173136	18.91561103971605
38	23.50283360391461	29.688105583093648	24.977411563119833	21.83164924987191
39	25.51383444265657	26.41767189710472	27.283951950525026	20.78454170971369
40	26.058236770015515	23.985020236599627	28.52406613462348	21.432676858761372
41	21.884925563151032	27.760798952712474	26.168449143319283	24.185826340817215
42	22.253000092393606	27.168279955027113	27.371965859962486	23.20675409261679
43	24.462527193118238	25.929545675439257	27.612369224049694	21.99555790739281
44	23.16601691162972	25.334446856277548	27.05794759002677	24.44158864206597
45	24.47050664094045	23.629484839649095	26.52914418322252	25.370864336187935
46	26.266662346965592	24.244202301200758	27.563652595240413	21.925482756593244
47	21.276987632455835	25.390063007639874	30.68745642801518	22.645492931889112
48	22.09317115255624	24.40073146938232	29.218158103459242	24.287939274602195
49	23.937923495694097	22.571098080012863	31.215779868033135	22.275198556259905
50	23.16319710676021	22.165406153894153	30.061999709620096	24.609397029725542
51	21.690778998093332	22.411869098658375	26.855101626967414	29.04225027628088
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	446.0
1	767.0
2	1088.0
3	5110.5
4	9133.0
5	6129.5
6	3126.0
7	3161.5
8	3197.0
9	3376.5
10	3556.0
11	3784.5
12	4013.0
13	3936.0
14	3859.0
15	3824.5
16	3790.0
17	3513.5
18	3237.0
19	3267.5
20	3298.0
21	3741.5
22	4185.0
23	4381.0
24	4577.0
25	5248.5
26	8176.5
27	10433.0
28	13037.0
29	15641.0
30	17315.0
31	18989.0
32	23003.0
33	27017.0
34	32197.5
35	37378.0
36	41180.0
37	44982.0
38	48168.5
39	51355.0
40	62754.5
41	74154.0
42	87625.5
43	101097.0
44	114250.5
45	127404.0
46	155323.0
47	183242.0
48	207881.0
49	232520.0
50	222691.5
51	212863.0
52	175887.0
53	138911.0
54	116124.5
55	93338.0
56	78683.5
57	64029.0
58	58224.5
59	52420.0
60	47567.5
61	42715.0
62	36325.5
63	29936.0
64	25564.0
65	21192.0
66	17568.5
67	13945.0
68	11126.0
69	8307.0
70	6575.0
71	4843.0
72	3732.5
73	2622.0
74	1968.0
75	1468.0
76	1622.0
77	1174.5
78	727.0
79	449.5
80	172.0
81	156.0
82	140.0
83	84.5
84	29.0
85	20.0
86	11.0
87	8.5
88	6.0
89	3.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1666782.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.645247453580637
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.83537370122127	14.6821308120953
2	13.054548034348798	5.912469412649664
3	6.252616321737932	4.247761315141578
4	3.5394838115271887	3.2060994707990385
5	2.3072569847770383	2.612420267963918
6	1.5406267894395005	2.0932724928283775
7	1.0686222381343684	1.6939450511866363
8	0.8255038428833756	1.4954991036780632
9	0.6012612984022738	1.225413979792261
>10	4.113094819999485	19.38325452394029
>50	1.2348173658528232	20.40301613509382
>100	0.6144203024254904	18.55097016905831
>500	0.008339329712308512	1.2612488428505675
>1k	0.002959116994690117	1.2826311044348289
>5k	8.070319076427593E-4	1.321253195159677
>10k+	2.690106358809198E-4	0.6286141233276181
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC	10319	0.6190971584766334	No Hit
GCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC	9972	0.5982785991209408	No Hit
CTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGCT	6072	0.3642947908004766	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5645	0.33867656358180015	No Hit
TCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC	3956	0.23734357582455295	No Hit
ACTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC	2960	0.17758771093040362	No Hit
CGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTG	2627	0.1576090934507332	No Hit
ACCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTG	1958	0.1174718709465305	No Hit
AGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTG	1777	0.10661262240652947	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.599750897237911E-4	0.0	0.0	0.38313348716268836	0.0
2	3.599750897237911E-4	0.0	0.0	2.1105939468988746	0.0
3	3.599750897237911E-4	0.0	0.0	2.9681146064692325	0.0
4	3.599750897237911E-4	0.0	0.0	3.9296080711214785	0.0
5	3.599750897237911E-4	0.0	0.0	6.330641919579165	0.0
6	3.599750897237911E-4	0.0	0.0	7.562116701524254	0.0
7	3.599750897237911E-4	0.0	0.0	8.95138056446494	0.0
8	3.599750897237911E-4	0.0	0.0	11.14446880275885	0.0
9	3.599750897237911E-4	0.0	0.0	11.93575404582003	0.0
10	3.599750897237911E-4	0.0	0.0	13.60537850780726	0.0
11	3.599750897237911E-4	0.0	0.0	16.063348416289593	0.0
12	3.599750897237911E-4	0.0	0.0	18.01267352299221	0.0
13	3.599750897237911E-4	0.0	0.0	18.738023328785648	0.0
14	3.599750897237911E-4	0.0	0.0	19.01226435130689	0.0
15	3.599750897237911E-4	0.0	0.0	19.509569937760308	0.0
16	3.599750897237911E-4	0.0	0.0	20.62639265362837	0.0
17	4.199709380110896E-4	0.0	0.0	22.0985707789021	0.0
18	4.199709380110896E-4	0.0	0.0	23.66938207876015	0.0
19	4.199709380110896E-4	0.0	0.0	24.6407748583798	0.0
20	4.199709380110896E-4	0.0	0.0	25.475617087297557	0.0
21	4.199709380110896E-4	0.0	0.0	26.57606093658319	0.0
22	4.199709380110896E-4	0.0	0.0	27.68400426690473	0.0
23	4.199709380110896E-4	0.0	0.0	28.844083989387933	0.0
24	4.199709380110896E-4	0.0	0.0	29.696684989398733	0.0
25	4.199709380110896E-4	0.0	0.0	30.471051403242896	0.0
26	4.199709380110896E-4	0.0	0.0	31.157103928408155	0.0
27	5.399626345856867E-4	0.0	0.0	31.82233789421772	0.0
28	6.599543311602837E-4	0.0	0.0	32.49639124972552	0.0
29	6.599543311602837E-4	0.0	0.0	33.19834267468691	0.0
30	6.599543311602837E-4	0.0	0.0	33.976608818669746	0.0
31	7.199501794475822E-4	0.0	0.0	34.705078408574124	0.0
32	7.199501794475822E-4	0.0	0.0	35.3650927355827	0.0
33	7.199501794475822E-4	0.0	0.0	36.01376784726497	0.0
34	7.199501794475822E-4	0.0	0.0	36.66442282194072	0.0
35	7.199501794475822E-4	0.0	0.0	37.363494446184326	0.0
36	7.199501794475822E-4	0.0	0.0	38.028428432752456	0.0
37	7.799460277348807E-4	0.0	0.0	38.643805848635274	0.0
38	7.799460277348807E-4	0.0	0.0	39.263862940684504	0.0
39	7.799460277348807E-4	0.0	0.0	39.8977790736881	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGAT	35	1.2123746E-7	45.000004	21
CGTTCAA	30	2.1660926E-6	45.000004	20
CTACGTT	30	2.1660926E-6	45.000004	30
CTTGCGA	30	2.1660926E-6	45.000004	21
CGCGTAA	35	1.2123746E-7	45.000004	31
GCGTAAC	35	1.2123746E-7	45.000004	32
CCGCAAT	35	1.2123746E-7	45.000004	33
CTTCGTA	25	3.891564E-5	45.0	22
GTACCGA	40	6.8175723E-9	45.0	36
CACGCAA	50	2.1827873E-11	45.0	45
GCACGGA	25	3.891564E-5	45.0	3
CGACATA	25	3.891564E-5	45.0	13
CCGCTAG	20	7.0342026E-4	45.0	1
CGTTCCG	20	7.0342026E-4	45.0	25
CGTTAGC	20	7.0342026E-4	45.0	12
ACGTCGC	20	7.0342026E-4	45.0	20
CGTCCGT	20	7.0342026E-4	45.0	30
CGATATC	25	3.891564E-5	45.0	10
ATACCGA	25	3.891564E-5	45.0	21
TCGAACG	20	7.0342026E-4	45.0	1
>>END_MODULE