##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934735.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2926164 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25080720014326 33.0 31.0 34.0 30.0 34.0 2 32.420253957057774 34.0 31.0 34.0 30.0 34.0 3 32.43725676346234 34.0 31.0 34.0 30.0 34.0 4 35.97191339924898 37.0 35.0 37.0 35.0 37.0 5 35.85819967711994 37.0 35.0 37.0 35.0 37.0 6 35.79338581159497 37.0 35.0 37.0 35.0 37.0 7 36.0910048103934 37.0 35.0 37.0 35.0 37.0 8 35.9198606093165 37.0 35.0 37.0 35.0 37.0 9 37.57906357948495 39.0 38.0 39.0 35.0 39.0 10 37.32383147356061 39.0 37.0 39.0 34.0 39.0 11 37.24449689080995 39.0 37.0 39.0 34.0 39.0 12 37.143836777432846 39.0 37.0 39.0 34.0 39.0 13 37.10337937313151 39.0 37.0 39.0 33.0 39.0 14 38.27357455016192 40.0 38.0 41.0 33.0 41.0 15 38.32679610575484 40.0 38.0 41.0 34.0 41.0 16 38.4139409137697 40.0 38.0 41.0 34.0 41.0 17 38.408332547321336 40.0 38.0 41.0 34.0 41.0 18 38.3899207289817 40.0 37.0 41.0 34.0 41.0 19 38.36281390926824 40.0 37.0 41.0 34.0 41.0 20 38.32733298612108 40.0 37.0 41.0 34.0 41.0 21 38.25583255073878 40.0 37.0 41.0 34.0 41.0 22 38.20317999948055 40.0 37.0 41.0 34.0 41.0 23 38.03095110185212 40.0 37.0 41.0 34.0 41.0 24 37.794866248098195 40.0 36.0 41.0 33.0 41.0 25 37.72018656507291 40.0 36.0 41.0 33.0 41.0 26 37.873106565455664 40.0 36.0 41.0 34.0 41.0 27 37.93157458023542 40.0 36.0 41.0 34.0 41.0 28 37.90186332686753 40.0 36.0 41.0 34.0 41.0 29 37.91193145702018 40.0 36.0 41.0 34.0 41.0 30 37.77555359166472 40.0 36.0 41.0 33.0 41.0 31 37.71190302389066 40.0 36.0 41.0 33.0 41.0 32 37.62742621397844 40.0 36.0 41.0 33.0 41.0 33 37.481432687983315 40.0 36.0 41.0 33.0 41.0 34 37.44352948091768 40.0 36.0 41.0 33.0 41.0 35 37.36701873169105 40.0 36.0 41.0 33.0 41.0 36 37.28688685938314 40.0 35.0 41.0 32.0 41.0 37 37.24057195700583 40.0 35.0 41.0 32.0 41.0 38 37.185541548593996 39.0 35.0 41.0 32.0 41.0 39 37.18842279516801 39.0 35.0 41.0 32.0 41.0 40 37.11281288403521 39.0 35.0 41.0 32.0 41.0 41 37.04393123556984 39.0 35.0 41.0 32.0 41.0 42 36.93632209267833 39.0 35.0 41.0 32.0 41.0 43 36.87100552122164 39.0 35.0 41.0 31.0 41.0 44 36.827013796902705 39.0 35.0 41.0 31.0 41.0 45 36.773979517210925 39.0 35.0 41.0 31.0 41.0 46 36.709855633518835 39.0 35.0 41.0 31.0 41.0 47 36.57384856077786 39.0 35.0 41.0 31.0 41.0 48 36.496557950955584 39.0 35.0 41.0 31.0 41.0 49 36.44333092745315 39.0 35.0 40.0 31.0 41.0 50 36.331628029051004 39.0 35.0 40.0 31.0 41.0 51 35.367576458462345 37.0 34.0 40.0 28.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 16.0 9 37.0 10 33.0 11 71.0 12 33.0 13 31.0 14 44.0 15 118.0 16 136.0 17 316.0 18 561.0 19 1061.0 20 1868.0 21 2839.0 22 4445.0 23 6560.0 24 9375.0 25 13632.0 26 18662.0 27 22825.0 28 26244.0 29 31061.0 30 38926.0 31 50001.0 32 65631.0 33 93096.0 34 170837.0 35 230767.0 36 221107.0 37 349260.0 38 630816.0 39 935116.0 40 639.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.96245596624113 22.504582791668547 25.928348513617145 11.604612728473182 2 30.874243548891993 25.052047663767308 26.206255015098268 17.86745377224243 3 31.766640557398695 24.530272397582635 26.818797579356456 16.88428946566221 4 28.466107846313466 26.49328609059506 26.4852892729184 18.555316790173073 5 25.466822775483532 30.19571015158412 25.347519824589465 18.98994724834288 6 26.508015271871294 34.624580167071976 23.903752489607555 14.963652071449173 7 81.44020635890537 5.989924009727411 7.881478960167645 4.6883906711995635 8 82.2502429802294 4.820714081644091 7.711768718363017 5.217274219763485 9 76.50528131711005 7.412708241916722 9.723959422643432 6.3580510183298 10 39.7910028282762 28.224050326639244 17.562925386273633 14.42202145881092 11 30.864674707227618 25.708299329770988 24.140239576455727 19.286786386545664 12 28.128430258864505 22.424682963770998 29.02988349251785 20.417003284846647 13 24.93349655043258 24.37860625720226 30.32550465387449 20.362392538490663 14 20.653797941605458 28.20163189759699 28.625770804370497 22.51879935642705 15 19.54217193568098 26.845556161582195 33.60686550719645 20.005406395540373 16 21.8921427507139 25.85032827961796 31.63117309897873 20.62635587068941 17 22.308831630763006 25.24697863824447 28.53291886579153 23.911270865200994 18 22.776235371633305 26.760427645203755 28.859079668808718 21.604257314354218 19 24.270307474222225 28.250091245740155 26.73872687928633 20.74087440075129 20 26.932393399686415 26.640851298833557 26.9660210432498 19.46073425823023 21 24.418727043323614 26.992813799910053 27.576615664740594 21.011843492025736 22 22.427997883919016 26.694744382064712 26.308812493079675 24.568445240936597 23 22.57231652087853 27.3942950565997 27.588542542386552 22.444845880135222 24 23.416425053414642 24.90868591097423 28.26820369603344 23.406685339577685 25 22.125212394110513 26.861173878155835 26.514200844518626 24.499412883215022 26 22.243285065362024 28.521333732490728 27.226806153038584 22.00857504910866 27 22.680102687340835 28.200094048043788 27.196322557450642 21.92348070716474 28 20.25259008039194 28.43285612152976 27.85366780535882 23.46088599271948 29 22.670294624634845 27.063042262839677 26.89247766017216 23.37418545235332 30 22.951994488347204 28.320798150753003 25.61582330997169 23.11138405092811 31 22.16560657570799 29.146281616478092 23.836087109266604 24.85202469854731 32 23.898763022168275 29.555520469802786 23.67345097540671 22.872265532622233 33 22.47895196578182 30.75145480567733 23.51655614654544 23.253037081995405 34 21.09632269414838 27.677635293168805 25.819297893077763 25.40674411960505 35 21.46434717944722 27.805481852691784 24.820481695489384 25.90968927237161 36 21.446268903588454 31.207956901937145 23.81349780805177 23.532276386422634 37 20.914993144608438 30.04855503655981 24.917742136120875 24.118709682710882 38 19.848819136589746 30.087855636252787 24.064577378438116 25.998747848719344 39 21.78890178404218 28.647164000377284 25.413784053115272 24.150150162465263 40 22.190109645255703 25.899710337493048 27.497775244313033 24.412404772938224 41 19.497164205423893 27.54927611712809 26.10530373553909 26.848255941908928 42 21.66033756139437 27.849908617562104 25.653483536807915 24.836270284235606 43 21.67605780127156 27.970202627057127 26.475412861343383 23.878326710327926 44 21.346650426975387 27.87099424365825 25.595660393607467 25.186694935758897 45 21.614783040185035 26.36332071613211 25.18242313144444 26.83947311223841 46 21.873825253813525 27.636865192791653 24.85096529107733 25.638344262317492 47 19.92154233323901 27.186480320310142 27.22906166571662 25.662915680734233 48 20.098258334119347 25.751256593957137 27.301272245848146 26.84921282607537 49 20.56846437861993 26.047514766773155 28.21130326256491 25.172717592042005 50 19.523888613215117 25.712947052865115 27.523679465675883 27.239484868243885 51 19.053614219845507 25.40667577073602 25.606937956997626 29.932772052420848 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 615.0 1 1356.5 2 2098.0 3 6711.5 4 11325.0 5 8005.0 6 4685.0 7 4746.5 8 4808.0 9 4821.0 10 4834.0 11 4898.5 12 4963.0 13 5130.0 14 5297.0 15 5294.5 16 5292.0 17 5251.0 18 5210.0 19 5713.5 20 6217.0 21 7324.5 22 8432.0 23 9198.5 24 9965.0 25 12137.5 26 17603.5 27 20897.0 28 24951.5 29 29006.0 30 35579.0 31 42152.0 32 48931.0 33 55710.0 34 63010.5 35 70311.0 36 78174.0 37 86037.0 38 95543.5 39 105050.0 40 117362.0 41 129674.0 42 146630.0 43 163586.0 44 184896.5 45 206207.0 46 236700.5 47 267194.0 48 293836.5 49 320479.0 50 312711.0 51 304943.0 52 265173.0 53 225403.0 54 198773.0 55 172143.0 56 156233.5 57 140324.0 58 131601.5 59 122879.0 60 122587.5 61 122296.0 62 108208.5 63 94121.0 64 77926.5 65 61732.0 66 48165.5 67 34599.0 68 28704.0 69 22809.0 70 19569.0 71 16329.0 72 13250.5 73 10172.0 74 8528.5 75 5546.5 76 4208.0 77 2860.5 78 1513.0 79 1202.5 80 892.0 81 600.5 82 309.0 83 213.0 84 117.0 85 97.5 86 78.0 87 54.5 88 31.0 89 24.0 90 17.0 91 11.5 92 6.0 93 3.0 94 0.0 95 2.0 96 4.0 97 2.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2926164.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.745785185961502 #Duplication Level Percentage of deduplicated Percentage of total 1 66.82169230654571 14.530901666605594 2 12.618800769708555 5.488114616850559 3 5.684516503044489 3.7084282428377566 4 3.1601643557902563 2.748810209333893 5 2.023461966222302 2.200088462471673 6 1.3450679966500847 1.7549735829398598 7 0.9183463037001125 1.3979113012609088 8 0.6809401306964031 1.1846062245299627 9 0.5344061681169534 1.0458973560531688 >10 4.0380750503107325 19.242078516791906 >50 1.4407462507505935 22.75416388139469 >100 0.7236744907400635 21.029163396732574 >500 0.00738023103646103 1.0836142015645285 >1k 0.002085717466825943 0.815144151626055 >5k 6.417592205618287E-4 1.0161041890069007 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC 9108 0.3112607495683769 No Hit GCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC 8270 0.2826225734442772 No Hit CTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGCT 5932 0.20272274554672945 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5814 0.19869016227388483 No Hit CGCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTG 3505 0.11978139297729042 No Hit TCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC 3223 0.11014420244388216 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.3922104160942448E-4 0.0 0.0 0.2170760080432949 0.0 2 2.3922104160942448E-4 0.0 0.0 1.0606035751926413 0.0 3 2.7339547612505654E-4 0.0 0.0 1.5395241004947091 0.0 4 3.075699106406886E-4 0.0 0.0 2.050978687455659 0.0 5 3.075699106406886E-4 0.0 0.0 3.446320848728916 0.0 6 3.075699106406886E-4 0.0 0.0 4.2802795742138855 0.0 7 3.075699106406886E-4 0.0 0.0 5.160134565253348 0.0 8 3.075699106406886E-4 0.0 0.0 6.6568380992999705 0.0 9 3.075699106406886E-4 0.0 0.0 7.17126586206378 0.0 10 3.075699106406886E-4 0.0 0.0 8.36528642960545 0.0 11 3.417443451563207E-4 0.0 0.0 10.0432853387575 0.0 12 3.417443451563207E-4 0.0 0.0 11.390270675191138 0.0 13 3.417443451563207E-4 0.0 0.0 11.905484449948807 0.0 14 3.417443451563207E-4 0.0 0.0 12.109403300703583 0.0 15 3.417443451563207E-4 0.0 0.0 12.495266840819585 0.0 16 3.7591877967195275E-4 0.0 0.0 13.339341198921181 0.0 17 3.7591877967195275E-4 0.0 0.0 14.419150806311608 0.0 18 3.7591877967195275E-4 0.0 0.0 15.563140001722392 0.0 19 3.7591877967195275E-4 0.0 0.0 16.242835329803796 0.0 20 3.7591877967195275E-4 0.0 0.0 16.931586883031848 0.0 21 3.7591877967195275E-4 0.0 0.0 17.793295249343508 0.0 22 3.7591877967195275E-4 0.0 0.0 18.74139658611069 0.0 23 3.7591877967195275E-4 0.0 0.0 19.649548008929095 0.0 24 3.7591877967195275E-4 0.0 0.0 20.363076027180977 0.0 25 3.7591877967195275E-4 0.0 0.0 21.001044370718798 0.0 26 3.7591877967195275E-4 0.0 0.0 21.579241628288777 0.0 27 3.7591877967195275E-4 0.0 0.0 22.156345303954254 0.0 28 4.100932141875848E-4 0.0 0.0 22.772339486098524 0.0 29 4.100932141875848E-4 0.0 0.0 23.388094447201183 0.0 30 4.100932141875848E-4 0.0 0.0 24.072642544983808 0.0 31 4.442676487032169E-4 0.0 0.0 24.730910502623914 0.0 32 4.442676487032169E-4 0.0 0.0 25.36887884616173 0.0 33 4.442676487032169E-4 0.0 0.0 25.98572738916889 0.0 34 4.442676487032169E-4 0.0 0.0 26.60155069914058 0.0 35 4.442676487032169E-4 0.0 0.0 27.245294522111543 0.0 36 4.442676487032169E-4 0.0 0.0 27.93038941084642 0.0 37 4.442676487032169E-4 0.0 0.0 28.565179531974284 0.0 38 4.442676487032169E-4 0.0 0.0 29.172322535579003 0.0 39 4.442676487032169E-4 0.0 0.0 29.786095379479757 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCGAT 30 2.1666565E-6 45.000004 42 AACGTCG 20 7.0350774E-4 45.0 11 CGTTTTT 4320 0.0 40.677086 1 TAACGCG 45 1.9301297E-8 40.0 1 CGTTCGG 40 3.4606637E-7 39.375 2 CTATCGG 35 6.251599E-6 38.57143 2 TAGATCG 35 6.251599E-6 38.57143 1 GCGTACG 170 0.0 38.38235 1 TTACGAG 200 0.0 38.25 1 TACGTTG 95 0.0 37.894737 1 TGCGTCG 95 0.0 37.894737 1 CGAGATA 530 0.0 37.35849 19 CTACGCG 85 0.0 37.058823 1 TTTAGCG 195 0.0 36.92308 1 ATCTCGC 55 2.752131E-9 36.81818 30 CGAATAT 315 0.0 36.428574 14 CGTTAGG 310 0.0 36.29032 2 TACGAAT 335 0.0 36.268658 12 TACGAGG 690 0.0 36.195652 2 CTACGTC 25 0.00210764 36.0 16 >>END_MODULE