##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934734.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1072079
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.098930209434194	33.0	31.0	34.0	30.0	34.0
2	32.287741854844654	34.0	31.0	34.0	30.0	34.0
3	32.284731815472554	34.0	31.0	34.0	30.0	34.0
4	35.86660124860202	37.0	35.0	37.0	35.0	37.0
5	35.75290347073303	37.0	35.0	37.0	35.0	37.0
6	35.678232667555285	37.0	35.0	37.0	33.0	37.0
7	36.016873756504886	37.0	35.0	37.0	35.0	37.0
8	35.877238524399786	37.0	35.0	37.0	35.0	37.0
9	37.56176643698832	39.0	38.0	39.0	35.0	39.0
10	37.29510884925458	39.0	37.0	39.0	34.0	39.0
11	37.17466250155073	39.0	37.0	39.0	34.0	39.0
12	36.99278504662436	39.0	35.0	39.0	33.0	39.0
13	36.84366077499886	39.0	35.0	39.0	33.0	39.0
14	37.905401560892436	40.0	37.0	41.0	33.0	41.0
15	38.00499496772159	40.0	37.0	41.0	33.0	41.0
16	38.11526296103179	40.0	37.0	41.0	34.0	41.0
17	38.107862387006925	40.0	37.0	41.0	34.0	41.0
18	38.09446785171615	40.0	37.0	41.0	34.0	41.0
19	38.03454969269988	40.0	36.0	41.0	34.0	41.0
20	37.96723282519292	40.0	36.0	41.0	34.0	41.0
21	37.88064592254862	40.0	36.0	41.0	34.0	41.0
22	37.79440041265616	39.0	35.0	41.0	33.0	41.0
23	37.62471235795123	39.0	35.0	41.0	33.0	41.0
24	37.362942469724715	39.0	35.0	41.0	33.0	41.0
25	37.29375820252052	39.0	35.0	41.0	33.0	41.0
26	37.45713702068598	39.0	35.0	41.0	33.0	41.0
27	37.49226129790809	39.0	35.0	41.0	33.0	41.0
28	37.49207008065637	39.0	35.0	41.0	33.0	41.0
29	37.50532096981659	39.0	35.0	41.0	33.0	41.0
30	37.238099057998525	39.0	35.0	41.0	33.0	41.0
31	37.15102804923891	39.0	35.0	41.0	32.0	41.0
32	37.04379528001201	39.0	35.0	41.0	32.0	41.0
33	36.81272462197282	39.0	35.0	41.0	31.0	41.0
34	36.78216250854648	39.0	35.0	41.0	31.0	41.0
35	36.66420851448447	39.0	35.0	41.0	31.0	41.0
36	36.50758013168806	39.0	35.0	41.0	31.0	41.0
37	36.46589010697905	39.0	35.0	40.0	31.0	41.0
38	36.3971031985516	39.0	35.0	40.0	30.0	41.0
39	36.42388760529774	39.0	35.0	41.0	31.0	41.0
40	36.365206295431584	39.0	35.0	40.0	30.0	41.0
41	36.32172535792605	39.0	35.0	40.0	30.0	41.0
42	36.24462376373383	39.0	35.0	40.0	30.0	41.0
43	36.17535741302647	39.0	35.0	40.0	30.0	41.0
44	36.075037380640794	38.0	35.0	40.0	30.0	41.0
45	35.99305461631093	38.0	35.0	40.0	30.0	41.0
46	35.90410128358078	38.0	35.0	40.0	29.0	41.0
47	35.787992302806046	38.0	35.0	40.0	29.0	41.0
48	35.78041263750153	38.0	35.0	40.0	29.0	41.0
49	35.79129336550758	38.0	35.0	40.0	29.0	41.0
50	35.67319292701377	38.0	35.0	40.0	29.0	41.0
51	34.66515060923682	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	18.0
10	24.0
11	15.0
12	8.0
13	16.0
14	17.0
15	29.0
16	51.0
17	160.0
18	273.0
19	480.0
20	923.0
21	1432.0
22	2117.0
23	3113.0
24	4712.0
25	7007.0
26	9691.0
27	11420.0
28	12844.0
29	14350.0
30	17402.0
31	21701.0
32	27476.0
33	39802.0
34	74219.0
35	102201.0
36	84736.0
37	129601.0
38	229114.0
39	276945.0
40	179.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.51514580548635	22.01917955673043	25.490378973937556	14.975295663845667
2	32.16012999042049	24.292612764544405	25.332461507034466	18.21479573800065
3	29.715254193021224	23.65338748357164	29.515642037573723	17.11571628583341
4	28.065655609334762	25.098243692862187	27.982825892494862	18.85327480530819
5	24.55789172253164	28.473461377379838	27.130649886808715	19.837997013279804
6	25.860687505305112	30.834294860733213	26.985791159047047	16.31922647491463
7	76.70041107045283	5.7306411187981485	11.60819305293733	5.960754757811691
8	76.93668097220447	4.323935083142194	11.462681388218591	7.27670255643474
9	71.4865229148225	5.94844223233549	14.565717638345681	7.99931721449632
10	40.30635802025784	22.654487215960764	21.47789481931835	15.56125994446305
11	29.60369525007019	23.234575063964503	26.249558101595127	20.91217158437018
12	27.21459892414645	19.900119300909726	32.2373631047712	20.647918670172626
13	21.98298819396705	25.963385160981606	32.39584023192321	19.657786413128136
14	17.421104228326456	28.96446996909743	30.80528580449762	22.8091399980785
15	16.339654074000144	24.76020890251558	38.055497775816896	20.84463924766738
16	18.50600562085443	24.325259612397968	33.526540488154325	23.642194278593276
17	17.96164275207331	24.53140113741618	30.230887835691213	27.2760682748193
18	19.91672255496097	23.888258234700988	33.66179171497623	22.533227495361814
19	20.251772490646676	26.526496648101492	30.022880776509943	23.19885008474189
20	22.644972991729155	26.08427177474794	29.931562879228117	21.339192354294788
21	21.01253732234285	26.851939082847437	30.92384050056013	21.211683094249583
22	18.72520588501407	26.065243326284726	28.32804298936925	26.88150779933195
23	18.73406717228861	25.973459045462132	29.585599568688504	25.706874213560756
24	21.491233388584234	23.159860420733917	30.5898166086641	24.759089582017744
25	18.614113325603803	26.05171820360253	29.336923864752507	25.997244606041157
26	19.2850526873486	26.756890117239497	30.48264167099626	23.475415524415645
27	21.17996901347755	25.958161665325036	30.19618890025828	22.66568042093913
28	19.187298697204216	24.607328377852753	32.117782364919	24.087590560024026
29	22.4466667101958	21.798020481699577	31.119348480848892	24.63596432725573
30	22.738995913547416	23.407323527463927	32.10239170807375	21.751288850914904
31	24.43075556931905	24.99703846451614	28.352947870446116	22.21925809571869
32	25.873000030781313	23.98181477297848	27.5309002414934	22.614284954746804
33	23.214893678544215	22.97713134946212	31.865002485824274	21.942972486169396
34	22.82537014529713	21.547665797016823	31.696451474191733	23.930512583494313
35	22.513266279817064	21.046863150943167	30.589070394998874	25.85080017424089
36	23.500693512325117	21.60596373961247	34.01661631279038	20.876726435272026
37	23.2697403829382	22.604677453807042	34.122112269711465	20.003469893543294
38	22.082421164858186	24.15269770231485	29.625335446361696	24.13954568646527
39	22.451330545603447	21.126894566538475	34.17518671665054	22.24658817120753
40	22.814643323859528	20.309137666160794	34.10924008398635	22.766978925993328
41	19.677374521840274	21.519962614695373	31.386492972999193	27.416169890465163
42	22.441629767955533	20.15112692254955	31.837952240459895	25.569291069035028
43	24.190941152657594	20.873461750486673	32.11339835963581	22.822198737219924
44	23.12422871821946	21.55857917187073	29.67057464981592	25.64661746009389
45	22.932451806256815	21.18892357746024	29.309966896096277	26.568657720186668
46	23.99524661895252	21.364936725745025	31.501969537692652	23.1378471176098
47	19.38028820637285	21.784495359017384	34.43132455723878	24.403891877370977
48	20.252985087852668	19.554622373910878	33.28588658111949	26.906505957116966
49	22.251904943572256	17.85166951316088	35.519863741384725	24.376561801882136
50	21.197598311318476	18.335589075058834	34.24999463658928	26.216817977033408
51	19.600048130781406	19.08665312910709	30.383301976813275	30.929996763298227
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	448.0
1	647.5
2	847.0
3	4894.0
4	8941.0
5	5967.0
6	2993.0
7	3043.5
8	3094.0
9	3267.0
10	3440.0
11	3417.0
12	3394.0
13	3358.0
14	3322.0
15	3222.0
16	3122.0
17	3000.0
18	2878.0
19	2951.5
20	3025.0
21	3027.0
22	3029.0
23	3130.5
24	3232.0
25	3573.0
26	4835.5
27	5757.0
28	6979.5
29	8202.0
30	9789.5
31	11377.0
32	12860.0
33	14343.0
34	17988.5
35	21634.0
36	24451.5
37	27269.0
38	29448.0
39	31627.0
40	35434.0
41	39241.0
42	48076.5
43	56912.0
44	68311.0
45	79710.0
46	95004.5
47	110299.0
48	126835.5
49	143372.0
50	140904.5
51	138437.0
52	114121.0
53	89805.0
54	75850.0
55	61895.0
56	53056.5
57	44218.0
58	40032.0
59	35846.0
60	33531.5
61	31217.0
62	27655.5
63	24094.0
64	21140.5
65	18187.0
66	15501.5
67	12816.0
68	10874.5
69	8933.0
70	6408.5
71	3884.0
72	3365.0
73	2846.0
74	2578.0
75	1727.0
76	1144.0
77	788.0
78	432.0
79	404.5
80	377.0
81	245.0
82	113.0
83	89.0
84	65.0
85	43.0
86	21.0
87	14.0
88	7.0
89	5.0
90	3.0
91	3.5
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1072079.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.558768126890463
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.85490666681042	16.418741509751005
2	12.24284029079392	6.013381518323202
3	5.881602696731807	4.333347505305904
4	3.422979926707163	3.3625668129200683
5	2.1371500603483433	2.6242886392232463
6	1.5168711098914571	2.2351491519721964
7	1.0076366044372402	1.7322419607179
8	0.7876950233469019	1.5475855546465709
9	0.5937623050363469	1.312386369468808
>10	3.844253335522105	19.553481438114005
>50	1.2879863191124956	22.919910295611782
>100	0.41389995062073276	13.786179349919655
>500	0.00420785532054335	0.7129502823671865
>1k	0.0026777261130730406	1.0893887830188294
>5k	0.0015301292074703092	2.3584008286395903
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGC	7896	0.7365128875763819	No Hit
GCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGC	6770	0.631483314196062	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5378	0.5016421364470341	No Hit
CTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGCT	5060	0.47198014325436843	No Hit
TCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGC	2731	0.2547386899659447	No Hit
CGCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTG	2241	0.20903310297095642	No Hit
ACTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGC	1993	0.1859004793490032	No Hit
GCCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTG	1353	0.12620338613105936	No Hit
ACCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTG	1192	0.11118583611842037	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCC	1078	0.10055229138897412	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.49986987899212654	0.0
2	0.0	0.0	0.0	2.4333094855882824	0.0
3	9.327670815303723E-5	0.0	0.0	3.390421787946597	0.0
4	9.327670815303723E-5	0.0	0.0	4.393892614256972	0.0
5	9.327670815303723E-5	0.0	0.0	6.943424878203938	0.0
6	9.327670815303723E-5	0.0	0.0	8.27756163491683	0.0
7	9.327670815303723E-5	0.0	0.0	9.82698103404693	0.0
8	9.327670815303723E-5	0.0	0.0	12.19415733355471	0.0
9	9.327670815303723E-5	0.0	0.0	13.055940840180622	0.0
10	9.327670815303723E-5	0.0	0.0	14.856740967783157	0.0
11	9.327670815303723E-5	0.0	0.0	17.604113129722716	0.0
12	9.327670815303723E-5	0.0	0.0	19.69351139235075	0.0
13	9.327670815303723E-5	0.0	0.0	20.527778270071515	0.0
14	9.327670815303723E-5	0.0	0.0	20.81814866255192	0.0
15	9.327670815303723E-5	0.0	0.0	21.40495243354268	0.0
16	9.327670815303723E-5	0.0	0.0	22.636671364703535	0.0
17	9.327670815303723E-5	0.0	0.0	24.152790979023003	0.0
18	9.327670815303723E-5	0.0	0.0	25.867869811832897	0.0
19	9.327670815303723E-5	0.0	0.0	26.77769082315762	0.0
20	9.327670815303723E-5	0.0	0.0	27.629120615178547	0.0
21	9.327670815303723E-5	0.0	0.0	28.682587757058947	0.0
22	9.327670815303723E-5	0.0	0.0	29.736894389312727	0.0
23	9.327670815303723E-5	0.0	0.0	30.818157990222737	0.0
24	9.327670815303723E-5	0.0	0.0	31.663991179754476	0.0
25	1.8655341630607447E-4	0.0	0.0	32.44154581891819	0.0
26	1.8655341630607447E-4	0.0	0.0	33.09560209648729	0.0
27	1.8655341630607447E-4	0.0	0.0	33.78090607128766	0.0
28	1.8655341630607447E-4	0.0	0.0	34.399610476466755	0.0
29	1.8655341630607447E-4	0.0	0.0	35.11438989104348	0.0
30	1.8655341630607447E-4	0.0	0.0	35.86358841092867	0.0
31	1.8655341630607447E-4	0.0	0.0	36.58032663637661	0.0
32	1.8655341630607447E-4	0.0	0.0	37.24156522047349	0.0
33	1.8655341630607447E-4	0.0	0.0	37.85719149428353	0.0
34	1.8655341630607447E-4	0.0	0.0	38.47001946684899	0.0
35	1.8655341630607447E-4	0.0	0.0	39.161199874263	0.0
36	2.798301244591117E-4	0.0	0.0	39.78074376981547	0.0
37	2.798301244591117E-4	0.0	0.0	40.41595815233765	0.0
38	2.798301244591117E-4	0.0	0.0	41.00947784631543	0.0
39	2.798301244591117E-4	0.0	0.0	41.60971346328022	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCTAG	115	0.0	45.000004	1
AACCGTA	20	7.0330757E-4	45.0	20
AAACTCG	20	7.0330757E-4	45.0	37
CGGGTAT	70	0.0	45.0	6
ACTATCG	20	7.0330757E-4	45.0	1
GATACCG	20	7.0330757E-4	45.0	33
GGTACGA	25	3.890629E-5	45.0	8
CGAACTA	20	7.0330757E-4	45.0	45
CACGGGT	25	3.890629E-5	45.0	4
ACGTTGA	25	3.890629E-5	45.0	9
TAGCGCG	75	0.0	45.0	1
ATTACCG	20	7.0330757E-4	45.0	1
ATTGTCG	20	7.0330757E-4	45.0	42
CGATTTA	20	7.0330757E-4	45.0	11
GTCTAGC	40	6.8139343E-9	45.0	12
ATATGCG	40	6.8139343E-9	45.0	1
GCCATTA	25	3.890629E-5	45.0	8
ACGGGTC	20	7.0330757E-4	45.0	5
CCATGCG	20	7.0330757E-4	45.0	1
GCACCGT	20	7.0330757E-4	45.0	16
>>END_MODULE