##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934733.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1809543
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16854752829858	33.0	31.0	34.0	30.0	34.0
2	32.345037393419226	34.0	31.0	34.0	30.0	34.0
3	32.35144508862182	34.0	31.0	34.0	30.0	34.0
4	35.912113721530794	37.0	35.0	37.0	35.0	37.0
5	35.79909568327473	37.0	35.0	37.0	35.0	37.0
6	35.728119751782636	37.0	35.0	37.0	33.0	37.0
7	36.07218783969212	37.0	35.0	37.0	35.0	37.0
8	35.90667864759224	37.0	35.0	37.0	35.0	37.0
9	37.59379080795538	39.0	38.0	39.0	35.0	39.0
10	37.30392038210753	39.0	37.0	39.0	34.0	39.0
11	37.194222519166445	39.0	37.0	39.0	34.0	39.0
12	37.14360918751309	39.0	37.0	39.0	34.0	39.0
13	37.083757059102766	39.0	37.0	39.0	33.0	39.0
14	38.24017500551244	40.0	37.0	41.0	33.0	41.0
15	38.31495355457152	40.0	38.0	41.0	34.0	41.0
16	38.412268733044755	40.0	38.0	41.0	34.0	41.0
17	38.40067851385681	40.0	38.0	41.0	34.0	41.0
18	38.38000975937018	40.0	37.0	41.0	34.0	41.0
19	38.35543007267581	40.0	37.0	41.0	34.0	41.0
20	38.28654748740428	40.0	37.0	41.0	34.0	41.0
21	38.226718016648405	40.0	37.0	41.0	34.0	41.0
22	38.1710553438078	40.0	37.0	41.0	34.0	41.0
23	37.977856287471475	40.0	36.0	41.0	34.0	41.0
24	37.72388166514971	40.0	36.0	41.0	33.0	41.0
25	37.65687911257152	39.0	36.0	41.0	33.0	41.0
26	37.81899297225874	40.0	36.0	41.0	33.0	41.0
27	37.90640343998457	40.0	36.0	41.0	34.0	41.0
28	37.88502124569574	40.0	36.0	41.0	34.0	41.0
29	37.87416712396445	40.0	36.0	41.0	34.0	41.0
30	37.74401989894686	40.0	36.0	41.0	33.0	41.0
31	37.66515799845596	40.0	36.0	41.0	33.0	41.0
32	37.52999956342568	40.0	36.0	41.0	33.0	41.0
33	37.36185545190139	40.0	36.0	41.0	32.0	41.0
34	37.34712466075689	40.0	36.0	41.0	33.0	41.0
35	37.27746232059697	40.0	36.0	41.0	32.0	41.0
36	37.20013450909981	40.0	35.0	41.0	32.0	41.0
37	37.13274843427318	39.0	35.0	41.0	32.0	41.0
38	37.01132385359176	39.0	35.0	41.0	31.0	41.0
39	37.021405957194716	39.0	35.0	41.0	32.0	41.0
40	36.974557664559505	39.0	35.0	41.0	31.0	41.0
41	36.93056865739029	39.0	35.0	41.0	31.0	41.0
42	36.85163381030459	39.0	35.0	41.0	31.0	41.0
43	36.78902021118039	39.0	35.0	41.0	31.0	41.0
44	36.72533949179434	39.0	35.0	41.0	31.0	41.0
45	36.67622211796017	39.0	35.0	41.0	31.0	41.0
46	36.63586220388242	39.0	35.0	41.0	31.0	41.0
47	36.498756315821176	39.0	35.0	41.0	31.0	41.0
48	36.45611571540439	39.0	35.0	41.0	31.0	41.0
49	36.42736702029186	39.0	35.0	41.0	31.0	41.0
50	36.3078898926414	39.0	35.0	40.0	31.0	41.0
51	35.40609258801808	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	12.0
10	16.0
11	21.0
12	16.0
13	22.0
14	32.0
15	37.0
16	96.0
17	177.0
18	333.0
19	694.0
20	1156.0
21	1888.0
22	2942.0
23	4410.0
24	6310.0
25	9088.0
26	12714.0
27	15653.0
28	17769.0
29	20623.0
30	25358.0
31	32213.0
32	41438.0
33	58478.0
34	102866.0
35	141958.0
36	135846.0
37	214927.0
38	388693.0
39	573350.0
40	399.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.503675789964646	22.254348197307277	25.387072868674576	12.854903144053498
2	30.725879407121027	24.583831387261867	26.617162454829757	18.073126750787353
3	30.130425195753848	24.250874392042633	28.653090863273214	16.96560954893031
4	28.394738340011816	26.0810049830261	27.48953741359006	18.034719263372022
5	24.975864071757346	29.42118534900801	26.58372859887828	19.019221980356367
6	26.908617258611706	32.784465470010936	25.00393745824222	15.30297981313514
7	79.84988474990647	5.237565506871071	9.65641601221966	5.256133731002801
8	80.40168152953537	4.472289412299127	9.092627254505697	6.033401803659819
9	74.7554493040508	6.90710306414382	11.394313370834515	6.943134260970864
10	37.13296672143188	33.278347074371815	17.11365797883775	12.475028225358557
11	25.734508657710816	23.46343800617062	31.03573664731924	19.766316688799325
12	25.329047168262925	21.228288026313827	33.54703369856367	19.895631106859575
13	21.16954391246851	24.138415058387668	35.113064458816396	19.578976570327423
14	17.55233227394983	28.676080093150592	31.62594091436346	22.145646718536117
15	16.018188017637602	26.76758717532548	37.34169345519835	19.872531351838557
16	18.72516983569885	25.581265546052233	34.611888194975194	21.08167642327372
17	18.87786032163922	24.730111414871047	30.55981537880006	25.83221288468967
18	19.227617138691926	25.884159702201053	32.98197390169783	21.90624925740919
19	20.986237961739512	26.98675853516606	29.791168267347057	22.235835235747366
20	23.112410149966042	27.00112680383942	30.568933703150464	19.317529343044075
21	22.089389420422727	27.230963840041383	30.461392738387538	20.218254001148356
22	20.164594043910533	25.30804739097109	29.075683749985494	25.45167481513288
23	18.64940484973278	27.13723851823361	30.751410715302153	23.461945916731462
24	21.271834932908476	23.965830046591876	31.661198435185018	23.101136585314634
25	19.08012133450269	27.07910229267832	29.03854730172204	24.802229071096956
26	18.397739097661674	28.49056363954877	29.22251640331288	23.889180859476674
27	22.318508043191017	26.960840388982188	29.10375713647037	21.616894431356425
28	18.340708123542797	26.574223436525134	31.2283267101141	23.85674172981797
29	19.998143177586826	25.185419744101132	29.52927893948914	25.287158138822896
30	22.88257311376408	26.13383600168661	27.868030767989488	23.115560116559816
31	22.288887304695162	27.65140148645266	25.49853747603677	24.561173732815412
32	21.808489767858514	30.29770500065486	24.487066624003962	23.406738607482662
33	22.294192511589944	30.397177629931978	25.349162744405636	21.95946711407245
34	20.40233362788284	26.43131442579701	27.341875821685363	25.82447612463478
35	19.577152905457346	28.43425107886356	25.59237332298818	26.39622269269092
36	22.044792524963487	29.44102461229161	25.44355121707525	23.070631645669653
37	22.04440568696074	28.518802813749105	28.087257390401888	21.349534108888264
38	19.848326345381125	31.23534505673532	25.9649535822028	22.951375015680757
39	20.398962611001785	29.27158956708959	27.294128959632346	23.035318862276277
40	20.66615714575448	26.66010147313438	29.068775928507918	23.604965452603228
41	17.95608062367128	28.496310947018117	26.523326607878346	27.02428182143226
42	21.564173937839552	27.23367170606059	26.98377435628775	24.218379999812107
43	23.05952386873371	26.68872748533746	27.286392199577463	22.96535644635137
44	21.033542723217963	26.72094556470888	27.058655141104687	25.18685657096847
45	20.629131222634665	25.70825893609602	26.535650161394344	27.12695967987497
46	22.26998750513251	26.712545653792148	26.943708991717795	24.073757849357545
47	19.062603099235552	26.261326754876784	30.24404504341704	24.43202510247062
48	19.832631774984073	25.164198916522018	28.941285175317745	26.061884133176168
49	20.516119263261498	24.538516078368957	30.880669870790584	24.064694787578965
50	19.566708279383246	24.596541778780608	29.407756544055598	26.42899339778054
51	18.639844424807812	24.22865883817074	26.413022514524386	30.71847422249706
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	446.0
1	976.0
2	1506.0
3	6207.5
4	10909.0
5	7577.5
6	4246.0
7	4240.0
8	4234.0
9	4396.5
10	4559.0
11	4335.5
12	4112.0
13	4082.5
14	4053.0
15	4237.5
16	4422.0
17	4400.0
18	4378.0
19	4791.5
20	5205.0
21	5243.0
22	5281.0
23	6642.5
24	8004.0
25	9865.5
26	15364.0
27	19001.0
28	21570.5
29	24140.0
30	27390.5
31	30641.0
32	35921.0
33	41201.0
34	45241.5
35	49282.0
36	55416.5
37	61551.0
38	64995.5
39	68440.0
40	75638.5
41	82837.0
42	94469.0
43	106101.0
44	120494.0
45	134887.0
46	156129.5
47	177372.0
48	198030.5
49	218689.0
50	209566.5
51	200444.0
52	166927.5
53	133411.0
54	114943.0
55	96475.0
56	84399.5
57	72324.0
58	66304.0
59	60284.0
60	57111.0
61	53938.0
62	48817.5
63	43697.0
64	34417.0
65	25137.0
66	20303.5
67	15470.0
68	12113.0
69	8756.0
70	7003.5
71	5251.0
72	4154.0
73	3057.0
74	2303.5
75	1538.0
76	1526.0
77	990.5
78	455.0
79	368.5
80	282.0
81	197.0
82	112.0
83	71.0
84	30.0
85	62.5
86	95.0
87	53.0
88	11.0
89	8.5
90	6.0
91	3.5
92	1.0
93	4.0
94	7.0
95	3.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1809543.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.405015476604365
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.85386532127123	13.881979909194259
2	12.797228895184704	5.478497651181543
3	6.05163778234846	3.886062011699176
4	3.58906301132393	3.0729579721558795
5	2.3342412479696706	2.498223501945955
6	1.5354234317527602	1.9719457391885293
7	1.113309473902014	1.6681284563386012
8	0.8357969269422	1.431219692519693
9	0.6256041743666724	1.2051960331092226
>10	4.037787313031682	18.031288019146462
>50	1.583051957375493	25.02141539421256
>100	0.6314475925787705	17.839009592392564
>500	0.006558449972918837	0.9086757633533088
>1k	0.003935069983751302	1.4735237119116413
>5k	0.001049351995667014	1.6318765516506308
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	9408	0.5199102756883921	No Hit
CCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	7927	0.438066406822054	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6035	0.33350962093744113	No Hit
CTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGCT	5691	0.31449929623114786	No Hit
TCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	3882	0.21452930380764645	No Hit
ACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	2698	0.1490984187720325	No Hit
CGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTG	2302	0.12721444033106702	No Hit
ACCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTG	2005	0.11080145650034291	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.33417277179928856	0.0
2	0.0	0.0	0.0	1.7360736937447743	0.0
3	0.0	0.0	0.0	2.4946630171264235	0.0
4	0.0	0.0	0.0	3.3192358512618934	0.0
5	0.0	0.0	0.0	5.5036547901873565	0.0
6	0.0	0.0	0.0	6.548614760743458	0.0
7	0.0	0.0	0.0	7.777985933464969	0.0
8	0.0	0.0	0.0	9.715546964067723	0.0
9	0.0	0.0	0.0	10.380134652782498	0.0
10	0.0	0.0	0.0	11.900021165565008	0.0
11	0.0	0.0	0.0	14.002596235624132	0.0
12	0.0	0.0	0.0	15.8378109832151	0.0
13	0.0	0.0	0.0	16.49841976675879	0.0
14	0.0	0.0	0.0	16.74268033420593	0.0
15	0.0	0.0	0.0	17.215562161274974	0.0
16	1.105251436412398E-4	0.0	0.0	18.192825481350816	0.0
17	1.105251436412398E-4	0.0	0.0	19.4893959414062	0.0
18	1.105251436412398E-4	0.0	0.0	20.966453961027728	0.0
19	1.105251436412398E-4	0.0	0.0	21.829102707147605	0.0
20	1.105251436412398E-4	0.0	0.0	22.665335943937226	0.0
21	1.105251436412398E-4	0.0	0.0	23.648843934628797	0.0
22	1.657877154618597E-4	0.0	0.0	24.694411793474927	0.0
23	2.210502872824796E-4	0.0	0.0	25.7315244788325	0.0
24	2.210502872824796E-4	0.0	0.0	26.50707941176308	0.0
25	2.210502872824796E-4	0.0	0.0	27.197529984089904	0.0
26	2.210502872824796E-4	0.0	0.0	27.85410459989069	0.0
27	2.210502872824796E-4	0.0	0.0	28.44618779437681	0.0
28	2.763128591030995E-4	0.0	0.0	29.09950191844018	0.0
29	2.763128591030995E-4	0.0	0.0	29.758287037113792	0.0
30	2.763128591030995E-4	0.0	0.0	30.529642014586003	0.0
31	3.315754309237194E-4	0.0	0.0	31.2390476490473	0.0
32	3.315754309237194E-4	0.0	0.0	31.90562479034762	0.0
33	3.315754309237194E-4	0.0	0.0	32.51732619783006	0.0
34	3.315754309237194E-4	0.0	0.0	33.1357143765028	0.0
35	3.315754309237194E-4	0.0	0.0	33.82041764136028	0.0
36	3.315754309237194E-4	0.0	0.0	34.480694849473046	0.0
37	4.421005745649592E-4	0.0	0.0	35.11356182196278	0.0
38	4.421005745649592E-4	0.0	0.0	35.73084474919911	0.0
39	4.973631463855791E-4	0.0	0.0	36.343872458405244	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGAAC	35	1.2124474E-7	45.000004	29
CGTAGAT	30	2.1661963E-6	45.000004	32
AACCGCG	25	3.8916973E-5	45.0	1
CGTATCC	25	3.8916973E-5	45.0	22
CGTATAG	25	3.8916973E-5	45.0	36
TACGCGA	25	3.8916973E-5	45.0	28
CGTAGTA	25	3.8916973E-5	45.0	15
TCTCGAT	25	3.8916973E-5	45.0	1
GTCGTTA	20	7.034363E-4	45.0	40
ACTCGTT	20	7.034363E-4	45.0	42
TCGTAGT	20	7.034363E-4	45.0	31
CGTAAGA	25	3.8916973E-5	45.0	25
GCGTGTA	20	7.034363E-4	45.0	5
CGCACCG	20	7.034363E-4	45.0	15
TGTCGCG	40	6.8193913E-9	45.0	1
CAATACG	20	7.034363E-4	45.0	1
GTAACGA	25	3.8916973E-5	45.0	23
TCGCGCG	25	3.8916973E-5	45.0	1
TCTCGAG	75	0.0	42.0	1
CGTTTTT	3865	0.0	41.856403	1
>>END_MODULE