##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934732.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1418821
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20879659943009	33.0	31.0	34.0	30.0	34.0
2	32.38655052328659	34.0	31.0	34.0	30.0	34.0
3	32.398103777714034	34.0	31.0	34.0	30.0	34.0
4	35.943409351849176	37.0	35.0	37.0	35.0	37.0
5	35.82266050474302	37.0	35.0	37.0	35.0	37.0
6	35.74263702045572	37.0	35.0	37.0	35.0	37.0
7	36.0337287085545	37.0	35.0	37.0	35.0	37.0
8	35.81330555440045	37.0	35.0	37.0	35.0	37.0
9	37.50252145971902	39.0	37.0	39.0	35.0	39.0
10	37.301905596266195	39.0	37.0	39.0	34.0	39.0
11	37.17197377259006	39.0	37.0	39.0	34.0	39.0
12	36.973048749630856	39.0	35.0	39.0	33.0	39.0
13	36.80566681773106	39.0	35.0	39.0	33.0	39.0
14	37.77963816436323	40.0	37.0	41.0	33.0	41.0
15	37.90176209683956	40.0	36.0	41.0	33.0	41.0
16	38.05701071523469	40.0	37.0	41.0	33.0	41.0
17	38.07408756989078	40.0	36.0	41.0	33.0	41.0
18	38.063158072794245	40.0	36.0	41.0	34.0	41.0
19	38.0395927322756	40.0	36.0	41.0	34.0	41.0
20	37.97424904198627	40.0	36.0	41.0	33.0	41.0
21	37.908208998880056	40.0	36.0	41.0	33.0	41.0
22	37.87274575157825	40.0	36.0	41.0	33.0	41.0
23	37.72663993555212	40.0	35.0	41.0	33.0	41.0
24	37.47051601294314	39.0	35.0	41.0	33.0	41.0
25	37.38008177211925	39.0	35.0	41.0	33.0	41.0
26	37.54158346965544	39.0	35.0	41.0	33.0	41.0
27	37.615751387948166	39.0	35.0	41.0	33.0	41.0
28	37.55315434434647	39.0	35.0	41.0	33.0	41.0
29	37.579306339559395	39.0	35.0	41.0	33.0	41.0
30	37.46840087650239	39.0	35.0	41.0	33.0	41.0
31	37.34834133410769	39.0	35.0	41.0	33.0	41.0
32	37.278596101974806	39.0	35.0	41.0	33.0	41.0
33	37.147102418134494	39.0	35.0	41.0	32.0	41.0
34	37.047631801333644	39.0	35.0	41.0	32.0	41.0
35	36.979105186630306	39.0	35.0	41.0	31.0	41.0
36	36.925684776303704	39.0	35.0	41.0	31.0	41.0
37	36.87038393144731	39.0	35.0	41.0	31.0	41.0
38	36.770568662290735	39.0	35.0	41.0	31.0	41.0
39	36.78655869908889	39.0	35.0	41.0	31.0	41.0
40	36.712091236315224	39.0	35.0	41.0	31.0	41.0
41	36.609112072629316	39.0	35.0	41.0	31.0	41.0
42	36.53506749618169	39.0	35.0	41.0	31.0	41.0
43	36.50497420041006	39.0	35.0	41.0	31.0	41.0
44	36.47949248002391	39.0	35.0	41.0	31.0	41.0
45	36.43062585061823	39.0	35.0	41.0	31.0	41.0
46	36.38360793926788	39.0	35.0	40.0	31.0	41.0
47	36.225517524761756	38.0	35.0	40.0	31.0	41.0
48	36.18471604240422	38.0	35.0	40.0	31.0	41.0
49	36.16552475611793	38.0	35.0	40.0	31.0	41.0
50	36.05549748699801	38.0	35.0	40.0	30.0	41.0
51	35.11096819119537	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	9.0
10	22.0
11	23.0
12	29.0
13	22.0
14	36.0
15	65.0
16	106.0
17	218.0
18	410.0
19	690.0
20	1106.0
21	1848.0
22	2825.0
23	4104.0
24	5896.0
25	8053.0
26	10354.0
27	12666.0
28	14326.0
29	17426.0
30	21270.0
31	27395.0
32	36738.0
33	53989.0
34	100387.0
35	122258.0
36	106249.0
37	164593.0
38	289074.0
39	416321.0
40	309.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.571120669908325	21.44245116191542	24.23646111806916	11.749967050107097
2	31.329181059485308	24.45946317400151	25.607035700768456	18.60432006574473
3	31.02364568892059	25.2931835657916	26.348989759807615	17.3341809854802
4	28.715532121388108	25.01725023804976	26.694346926074537	19.572870714487593
5	26.14135257372142	28.278267660261587	25.972761891739694	19.607617874277306
6	26.63161878771177	32.82739683159468	24.757245628588805	15.783738752104739
7	79.06747926623584	5.952547925354925	9.508458078926093	5.471514729483141
8	78.97409186923508	5.935421029150259	9.05716788798587	6.033319213628781
9	73.39974528146962	6.851322330301003	11.827637171989982	7.921295216239399
10	42.78693365829798	24.169433635391638	18.56858617119425	14.475046535116128
11	32.16550925028598	24.62354306850547	24.460802314033973	18.750145367174575
12	29.400396526411722	22.215416884864265	28.571398365262425	19.81278822346159
13	25.60907965134432	25.069406218261502	30.538665554005757	18.782848576388425
14	20.116843491885163	28.267484058947534	30.151936008841147	21.46373644032616
15	18.56153806576023	24.901731789986194	35.444781265571905	21.091948878681666
16	21.759052058011548	25.38198969426023	31.481349655805772	21.37760859192245
17	22.811757085636593	24.640458521547114	28.121235871191647	24.426548521624643
18	23.81484345100615	23.682198106737918	30.558259287112328	21.944699155143603
19	24.34204173747076	26.56882016829466	26.576925489543786	22.512212604690795
20	25.023311608723013	27.669381831816697	27.522358352463066	19.784948206997218
21	23.7196940276469	26.906847304910205	29.16611750178493	20.207341165657965
22	21.196683725431186	24.696702402910585	29.118542790105305	24.988071081552924
23	20.936749597024573	26.50390711724735	29.200018888922564	23.359324396805516
24	22.905496887909045	23.947277352111364	29.44226227268979	23.7049634872898
25	22.197726140224876	26.22212386199528	27.066204968773366	24.51394502900648
26	20.0411468395238	26.77497725223971	27.844386289743383	25.3394896184931
27	22.48754423567173	26.678629650956676	27.884701452825972	22.94912466054562
28	17.980492253779722	25.967828217935878	30.144746941298443	25.906932586985953
29	20.608025959581937	24.4322574870262	28.533691001190427	26.426025552201445
30	24.067306587652705	24.062866281229276	27.91014511344278	23.959682017675238
31	22.071071685575557	25.480592689282155	28.13815132423329	24.310184300908993
32	23.846700887567916	26.125423855440538	25.145243832731545	24.88263142426
33	23.619258525212132	25.73242149643965	25.84822186872058	24.80009810962764
34	19.86360506364087	24.2742389631955	30.343644476646453	25.518511496517178
35	20.169845244748984	27.158605631013355	26.588554863509916	26.082994260727745
36	22.80611860128938	27.442644280004313	25.82594985554908	23.925287263157227
37	21.086028470117093	26.903746138519235	29.754422862362485	22.255802529001194
38	20.06299596636926	27.718648088800492	27.182357746325998	25.03599819850425
39	20.079981900465246	26.044582086112346	27.98626465213018	25.889171361292227
40	22.065221758065324	24.11748909834292	29.239276836190047	24.578012307401707
41	18.849382691685562	26.110481871920417	27.771790803772994	27.268344632621027
42	20.38121792671521	25.491517252704888	29.17239031562121	24.954874504958692
43	21.4936204073664	24.688456119552786	28.376024882631423	25.441898590449398
44	20.73672436480712	24.438882706134176	28.151824648775285	26.67256828028342
45	20.372830681248725	23.515721856386392	27.05528040535064	29.056167057014235
46	22.087987138617205	24.460167984544913	27.670086642360097	25.78175823447778
47	17.92692665248118	24.968759272663714	31.774268917643596	25.33004515721152
48	19.368898543227086	24.643630168992424	29.866487738763382	26.120983549017108
49	20.983478536052118	22.947010228915417	31.193152624608743	24.876358610423726
50	20.4157536433419	23.729561375254523	29.436059939907857	26.41862504149572
51	19.2387905169151	23.84761714127434	26.911569535550996	30.002022806259564
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	358.0
1	773.5
2	1189.0
3	3929.5
4	6670.0
5	4590.0
6	2510.0
7	2447.5
8	2385.0
9	2453.0
10	2521.0
11	2455.0
12	2389.0
13	2507.5
14	2626.0
15	2575.5
16	2525.0
17	2677.0
18	2829.0
19	2775.5
20	2722.0
21	2971.0
22	3220.0
23	4074.5
24	4929.0
25	5762.5
26	7468.5
27	8341.0
28	10558.5
29	12776.0
30	15898.0
31	19020.0
32	21045.5
33	23071.0
34	27001.0
35	30931.0
36	35357.0
37	39783.0
38	43657.5
39	47532.0
40	56235.0
41	64938.0
42	72708.0
43	80478.0
44	90051.0
45	99624.0
46	115823.0
47	132022.0
48	143537.0
49	155052.0
50	153273.5
51	151495.0
52	132133.0
53	112771.0
54	101859.5
55	90948.0
56	80659.0
57	70370.0
58	65432.0
59	60494.0
60	54110.0
61	47726.0
62	43526.5
63	39327.0
64	35252.5
65	31178.0
66	26132.0
67	21086.0
68	18672.5
69	16259.0
70	12278.0
71	8297.0
72	6682.5
73	5068.0
74	4251.5
75	2368.0
76	1301.0
77	1275.0
78	1249.0
79	874.5
80	500.0
81	320.5
82	141.0
83	101.0
84	61.0
85	59.5
86	58.0
87	34.5
88	11.0
89	8.0
90	5.0
91	4.0
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1418821.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.24967331588072
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.94459795479943	16.96133650614505
2	11.909897639184459	5.776222539516043
3	5.0224172959170605	3.6537593604605325
4	2.7120643839140652	2.6306670128620557
5	1.6492500315724123	1.9996887240918482
6	1.10947241193654	1.6142606125466004
7	0.7916510438263649	1.3438095435075725
8	0.5928489304821778	1.1501114311889673
9	0.4352442004992349	0.9499076707264322
>10	3.8590042432783718	21.675943188820714
>50	1.6566689056887278	28.88883751295011
>100	0.30807249665477876	10.01801626243525
>500	0.00469891319807173	0.745926268791151
>1k	0.0038178669734332802	2.136935614029719
>5k	2.936820748794831E-4	0.45457775192800426
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC	6383	0.4498805698534205	No Hit
CCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC	4083	0.28777414487098796	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3459	0.2437939669627106	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGC	3304	0.23286940353998142	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCG	2995	0.21109075774886332	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTC	2969	0.20925825033601841	No Hit
CTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGCT	2932	0.20665045132543147	No Hit
TCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC	2103	0.14822165727741554	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1576	0.11107814164013642	No Hit
ACTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC	1570	0.1106552553140953	No Hit
GAGCGCCATTATTCCTGTCAGTTTAAGTTATGTTGGTGGTTGTTAAACAGG	1449	0.10212704773893255	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.8192421736075236E-4	0.0	0.0	0.2182798252915625	0.0
2	3.524052717009404E-4	0.0	0.0	1.2768347804268474	0.0
3	3.524052717009404E-4	0.0	0.0	1.8363838708336007	0.0
4	3.524052717009404E-4	0.0	0.0	2.488967952969402	0.0
5	3.524052717009404E-4	0.0	0.0	4.334514360867227	0.0
6	3.524052717009404E-4	0.0	0.0	5.212778779000311	0.0
7	3.524052717009404E-4	0.0	0.0	6.248920758855416	0.0
8	3.524052717009404E-4	0.0	0.0	7.861386320050239	0.0
9	3.524052717009404E-4	0.0	0.0	8.414028267131654	0.0
10	3.524052717009404E-4	0.0	0.0	9.858466994779468	0.0
11	3.524052717009404E-4	0.0	0.0	11.599983366471175	0.0
12	4.2288632604112853E-4	0.0	0.0	13.226263214316676	0.0
13	4.2288632604112853E-4	0.0	0.0	13.78214728989774	0.0
14	4.2288632604112853E-4	0.0	0.0	14.004374054232352	0.0
15	4.2288632604112853E-4	0.0	0.0	14.492032469212113	0.0
16	4.2288632604112853E-4	0.0	0.0	15.374102864279568	0.0
17	4.2288632604112853E-4	0.0	0.0	16.41334601052564	0.0
18	7.048105434018808E-4	0.0	0.0	17.55633726876047	0.0
19	7.048105434018808E-4	0.0	0.0	18.29793892252793	0.0
20	7.048105434018808E-4	0.0	0.0	18.971103472531066	0.0
21	7.048105434018808E-4	0.0	0.0	19.79023428607273	0.0
22	7.048105434018808E-4	0.0	0.0	20.68555511935614	0.0
23	7.75291597742069E-4	0.0	0.0	21.491999343116575	0.0
24	7.75291597742069E-4	0.0	0.0	22.150644795925633	0.0
25	7.75291597742069E-4	0.0	0.0	22.72774366886309	0.0
26	7.75291597742069E-4	0.0	0.0	23.260791882837935	0.0
27	7.75291597742069E-4	0.0	0.0	23.865448848022407	0.0
28	7.75291597742069E-4	0.0	0.0	24.40977403069168	0.0
29	7.75291597742069E-4	0.0	0.0	25.002731140855683	0.0
30	7.75291597742069E-4	0.0	0.0	25.69887251457372	0.0
31	7.75291597742069E-4	0.0	0.0	26.25031628373135	0.0
32	7.75291597742069E-4	0.0	0.0	26.781038622912966	0.0
33	7.75291597742069E-4	0.0	0.0	27.339037130124236	0.0
34	7.75291597742069E-4	0.0	0.0	27.914655900920554	0.0
35	7.75291597742069E-4	0.0	0.0	28.52699530102811	0.0
36	7.75291597742069E-4	0.0	0.0	29.05926822340521	0.0
37	7.75291597742069E-4	0.0	0.0	29.624667241322197	0.0
38	7.75291597742069E-4	0.0	0.0	30.216849059888457	0.0
39	9.162537064224451E-4	0.0	0.0	31.0875015241528	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACTTA	20	7.033846E-4	45.000004	16
TCGCGAT	20	7.033846E-4	45.000004	40
GTTAACG	35	1.2121927E-7	45.0	1
GTTCGCA	25	3.89127E-5	45.0	29
ATACCCG	25	3.89127E-5	45.0	1
AACCGCA	30	2.1658634E-6	44.999996	37
CGACGGT	30	2.1658634E-6	44.999996	28
TATTGCG	95	0.0	42.63158	1
GCGTCAG	75	0.0	42.0	1
CGTTTTT	2085	0.0	40.89928	1
CTCGATC	50	1.0822987E-9	40.5	16
ATAACGG	150	0.0	40.5	2
ACGTAAG	50	1.0822987E-9	40.5	1
ACGATAG	95	0.0	40.263157	1
CAGTTCG	45	1.9288564E-8	40.0	1
TTTAGCG	125	0.0	39.600002	1
GCCGACG	40	3.4589902E-7	39.375004	1
CGTAAGG	195	0.0	39.230766	2
ACTACCG	35	6.2493273E-6	38.571426	1
AATTACG	35	6.2493273E-6	38.571426	1
>>END_MODULE