##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934731.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2983357
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28918798521263	33.0	31.0	34.0	30.0	34.0
2	32.487647639890234	34.0	31.0	34.0	31.0	34.0
3	32.44491423587589	34.0	31.0	34.0	30.0	34.0
4	36.008015131947	37.0	35.0	37.0	35.0	37.0
5	35.915318884062486	37.0	35.0	37.0	35.0	37.0
6	35.86086445571214	37.0	35.0	37.0	35.0	37.0
7	36.15114885680795	37.0	35.0	37.0	35.0	37.0
8	36.006874135411884	37.0	36.0	37.0	35.0	37.0
9	37.71680358736819	39.0	38.0	39.0	35.0	39.0
10	37.45414243082541	39.0	37.0	39.0	35.0	39.0
11	37.35855916673734	39.0	37.0	39.0	34.0	39.0
12	37.3190292009974	39.0	37.0	39.0	34.0	39.0
13	37.33503767735474	39.0	37.0	39.0	34.0	39.0
14	38.575230185324784	40.0	38.0	41.0	34.0	41.0
15	38.623126900334086	40.0	38.0	41.0	35.0	41.0
16	38.66988597073699	40.0	38.0	41.0	35.0	41.0
17	38.63457608325118	40.0	38.0	41.0	35.0	41.0
18	38.52935635929592	40.0	38.0	41.0	35.0	41.0
19	38.41533614649538	40.0	37.0	41.0	34.0	41.0
20	38.30293458007205	40.0	37.0	41.0	34.0	41.0
21	38.23428506880001	40.0	37.0	41.0	34.0	41.0
22	38.19238160233589	40.0	36.0	41.0	34.0	41.0
23	38.0133222406839	40.0	36.0	41.0	34.0	41.0
24	37.79156165353325	40.0	36.0	41.0	33.0	41.0
25	37.722041981566406	40.0	36.0	41.0	33.0	41.0
26	37.88207646620904	40.0	36.0	41.0	34.0	41.0
27	37.95683017486677	40.0	36.0	41.0	34.0	41.0
28	37.89965163404849	40.0	36.0	41.0	34.0	41.0
29	37.88885942915984	40.0	36.0	41.0	34.0	41.0
30	37.73241083785816	40.0	36.0	41.0	33.0	41.0
31	37.580432713885735	40.0	36.0	41.0	33.0	41.0
32	37.35137028521897	40.0	36.0	41.0	33.0	41.0
33	37.05694993927981	40.0	35.0	41.0	32.0	41.0
34	36.83623381311724	40.0	35.0	41.0	31.0	41.0
35	36.67750993260277	40.0	35.0	41.0	31.0	41.0
36	36.54995831876641	40.0	35.0	41.0	30.0	41.0
37	36.4840661040566	40.0	35.0	41.0	30.0	41.0
38	36.430564629040376	39.0	35.0	41.0	30.0	41.0
39	36.42257363098013	39.0	35.0	41.0	30.0	41.0
40	36.36252215205891	39.0	35.0	41.0	30.0	41.0
41	36.27228890139531	39.0	35.0	41.0	30.0	41.0
42	36.16596940962815	39.0	35.0	41.0	29.0	41.0
43	36.11315776154178	39.0	35.0	41.0	29.0	41.0
44	36.067021144301535	39.0	35.0	41.0	29.0	41.0
45	36.010210645256336	39.0	35.0	41.0	29.0	41.0
46	35.96065472553234	39.0	35.0	41.0	29.0	41.0
47	35.85458998034764	39.0	35.0	41.0	28.0	41.0
48	35.78801028505807	39.0	35.0	41.0	28.0	41.0
49	35.74448783702386	39.0	35.0	41.0	28.0	41.0
50	35.64373623404775	39.0	35.0	41.0	27.0	41.0
51	34.75246073466903	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	32.0
10	37.0
11	44.0
12	28.0
13	32.0
14	58.0
15	83.0
16	178.0
17	325.0
18	616.0
19	1262.0
20	2107.0
21	3700.0
22	5861.0
23	9237.0
24	15194.0
25	27203.0
26	40065.0
27	44344.0
28	41625.0
29	39870.0
30	41675.0
31	48905.0
32	61423.0
33	84953.0
34	153700.0
35	205564.0
36	209803.0
37	325642.0
38	621317.0
39	997774.0
40	690.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.73008057701442	20.997989848348688	25.057309601231097	16.214619973405796
2	35.18093878808335	23.06750415722959	25.546691193846392	16.204865860840656
3	29.548257214942765	22.44143091155366	32.0764829686826	15.93382890482098
4	26.08410592496976	24.60262047083202	32.12320885499121	17.190064749207018
5	24.44957140563466	28.200647793743762	29.774143691150606	17.575637109470975
6	25.167051747410717	32.263017801758224	28.104413920291805	14.465516530539254
7	78.09464304808309	4.810051227526575	12.48861601209644	4.606689712293902
8	80.09530874112619	3.585893340957854	11.861738303528542	4.457059614387417
9	74.29405867283064	6.043125244481301	13.96172164444282	5.701094438245239
10	36.47552069698665	28.580555394476757	21.04649225687707	13.897431651659522
11	25.232984185265124	23.338574632536435	32.16058286018067	19.26785832201778
12	23.573947067012092	21.80644153549173	34.09662336756882	20.52298802992736
13	21.777648467816626	22.732780555595593	36.159098626144974	19.330472350442808
14	18.864520739556145	25.59127184577642	34.76271864212027	20.781488772547167
15	18.34175393692408	25.59646733528706	36.48520777097745	19.576570956811405
16	21.39462357337724	24.776049262625964	34.815142807247	19.014184356749794
17	21.23352987926018	24.81178082274431	32.40044687913649	21.554242418859022
18	21.657817016200205	24.80584790891603	33.38537761320553	20.15095746167824
19	21.45311472948092	26.67665988348025	31.698284851595034	20.171940535443795
20	22.930477311297306	25.92150386293025	31.55381672391202	19.59420210186042
21	21.848642318033008	26.914579783780486	32.30756493440108	18.929212963785428
22	21.550421220122164	23.675912738569334	32.02405880355586	22.749607237752638
23	20.206968190531676	26.145312143333836	32.183945803334964	21.463773862799524
24	20.47508896856796	23.955966382836515	33.650247020386765	21.918697628208758
25	20.024053440469913	26.109412986779656	31.70850823418049	22.15802533856994
26	19.39580144112823	26.918535059666006	32.69397527684417	20.99168822236159
27	20.579099316642292	26.135356915045705	32.05261723622081	21.2329265320912
28	19.388125524367346	26.48238209506941	32.33622392492752	21.793268455635715
29	20.847555287550232	24.601547853642725	32.29529687529853	22.25559998350851
30	21.33475812649978	24.948573033666438	32.109868178699365	21.60680066113442
31	21.96780338390612	26.41410330711343	29.81785284161433	21.800240467366123
32	23.092341949019175	26.52277283610376	29.813093102836834	20.571792112040228
33	22.501329877718288	26.90023352887368	28.547740012341798	22.05069658106623
34	21.26584247208765	26.724558944839654	30.437725019164652	21.57187356390804
35	21.8217933690135	26.775407703469618	29.68320586507079	21.719593062446098
36	21.533125267944804	29.209075548115766	28.17872617993757	21.079073004001867
37	22.577284582435155	29.440827899577553	28.062380734186355	19.919506783800934
38	22.726814122480146	27.52137943933629	27.714651649132172	22.037154789051392
39	22.322605038552208	26.9874842333653	27.844371290462387	22.845539437620104
40	22.354180207062043	25.895660492525703	29.91730456663416	21.83285473377809
41	20.590797547863026	27.808136941036558	28.80748096858673	22.793584542513685
42	21.77533563700221	27.64231702742917	28.812877573820366	21.769469761748258
43	22.20411435842241	26.691508927694542	29.352906809342628	21.75146990454042
44	22.304973893503192	25.87202939507407	28.58786259907882	23.235134112343914
45	21.904518969737783	24.44444295469835	28.85279904483439	24.798239030729476
46	21.83778206899141	25.951470105656142	28.285920860292617	23.924826965059832
47	20.817454967675676	26.09489913543703	30.427501636579198	22.6601442603081
48	21.25793192031661	24.946662434298005	30.25836331354243	23.537042331842954
49	21.804631493984797	24.40649912162708	30.411579975175616	23.377289409212505
50	20.260163299263212	24.294946933940523	31.033262194232876	24.41162757256339
51	20.336888947584885	23.61346630658014	29.12024943712737	26.929395308707605
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	438.0
1	1121.0
2	1804.0
3	23982.5
4	46161.0
5	32247.0
6	18333.0
7	17977.0
8	17621.0
9	17794.5
10	17968.0
11	17553.5
12	17139.0
13	16567.5
14	15996.0
15	15196.5
16	14397.0
17	14117.0
18	13837.0
19	13846.0
20	13855.0
21	13978.5
22	14102.0
23	15135.5
24	16169.0
25	18327.5
26	23399.5
27	26313.0
28	33480.0
29	40647.0
30	46269.5
31	51892.0
32	59028.5
33	66165.0
34	73830.0
35	81495.0
36	90042.5
37	98590.0
38	106104.0
39	113618.0
40	123860.5
41	134103.0
42	148018.0
43	161933.0
44	180770.5
45	199608.0
46	232248.0
47	264888.0
48	287776.0
49	310664.0
50	305213.5
51	299763.0
52	255699.0
53	211635.0
54	184817.5
55	158000.0
56	142046.5
57	126093.0
58	115968.0
59	105843.0
60	100525.0
61	95207.0
62	85440.5
63	75674.0
64	64183.0
65	52692.0
66	41390.5
67	30089.0
68	24687.5
69	19286.0
70	15555.5
71	11825.0
72	9778.0
73	7731.0
74	6501.0
75	4395.5
76	3520.0
77	2443.5
78	1367.0
79	984.0
80	601.0
81	478.0
82	355.0
83	232.0
84	109.0
85	73.0
86	37.0
87	24.5
88	12.0
89	11.0
90	10.0
91	7.0
92	4.0
93	5.0
94	6.0
95	4.5
96	3.0
97	2.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2983357.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.802145101530822
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.49010493090353	19.428903107832713
2	11.416876955063193	6.119935855118543
3	4.580077216997642	3.682676824385589
4	2.2504639660360883	2.412690470538631
5	1.3719881634622342	1.8386112917348798
6	0.9030165749892602	1.4521668763169728
7	0.6442460663463897	1.2087023585914474
8	0.48736913849676833	1.0450030694398764
9	0.3745044256330671	0.9033769761284631
>10	3.874952415514227	24.93530807019707
>50	1.4212271892691093	26.480732461319374
>100	0.17940212714762901	6.602991883327134
>500	0.003462498084536048	0.5823864667494081
>1k	0.0012824066979763116	0.6706518377723344
>5k	7.694440187857869E-4	1.3942010299077081
>10k+	2.564813395952623E-4	1.2416614206398924
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25661	0.8601384279521358	No Hit
CGCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTG	10464	0.3507458208990744	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTT	8713	0.292053549072404	No Hit
CCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC	7185	0.2408360782836248	Illumina Single End Adapter 1 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTC	7076	0.23718247598259276	No Hit
CTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGCT	6005	0.20128331942841574	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC	5924	0.19856825716801577	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCT	5660	0.18971916535634187	No Hit
CGTCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCT	3928	0.13166375998581462	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.340771486617257E-4	0.0	0.0	0.21217708775718092	0.0
2	1.340771486617257E-4	0.0	0.0	0.8142840431098256	0.0
3	1.340771486617257E-4	0.0	0.0	1.4431393896204845	0.0
4	1.340771486617257E-4	0.0	0.0	1.9595040084039557	0.0
5	1.340771486617257E-4	0.0	0.0	3.291292326060877	0.0
6	1.340771486617257E-4	0.0	0.0	4.577460893885647	0.0
7	1.340771486617257E-4	0.0	0.0	5.650111602466617	0.0
8	1.340771486617257E-4	0.0	0.0	7.19679207014112	0.0
9	1.340771486617257E-4	0.0	0.0	7.901535082794315	0.0
10	1.340771486617257E-4	0.0	0.0	9.146977716713085	0.0
11	1.340771486617257E-4	0.0	0.0	10.705490492756985	0.0
12	1.340771486617257E-4	0.0	0.0	11.9315924979813	0.0
13	1.340771486617257E-4	0.0	0.0	12.470046327006791	0.0
14	1.340771486617257E-4	0.0	0.0	12.717552743436338	0.0
15	1.340771486617257E-4	0.0	0.0	13.063605864132251	0.0
16	1.340771486617257E-4	0.0	0.0	13.756483049128883	0.0
17	1.340771486617257E-4	0.0	0.0	14.657347410987018	0.0
18	1.340771486617257E-4	0.0	0.0	15.621194513429	0.0
19	1.340771486617257E-4	0.0	0.0	16.24371471466539	0.0
20	1.340771486617257E-4	0.0	0.0	16.848235058693948	0.0
21	1.340771486617257E-4	0.0	0.0	17.60091065199371	0.0
22	1.340771486617257E-4	0.0	0.0	18.394044024902147	0.0
23	1.340771486617257E-4	0.0	0.0	19.228774833182886	0.0
24	1.340771486617257E-4	0.0	0.0	19.86976416164743	0.0
25	1.340771486617257E-4	0.0	0.0	20.447670191666635	0.0
26	1.340771486617257E-4	0.0	0.0	20.996716115436403	0.0
27	1.6759643582715713E-4	0.0	0.0	21.527661624136837	0.0
28	1.6759643582715713E-4	0.0	0.0	22.092193458577032	0.0
29	1.6759643582715713E-4	0.0	0.0	22.678345233238932	0.0
30	1.6759643582715713E-4	0.0	0.0	23.376585504182035	0.0
31	1.6759643582715713E-4	0.0	0.0	24.004837503523714	0.0
32	1.6759643582715713E-4	0.0	0.0	24.619614749424894	0.0
33	1.6759643582715713E-4	0.0	0.0	25.192694002092274	0.0
34	1.6759643582715713E-4	0.0	0.0	25.73242156403005	0.0
35	1.6759643582715713E-4	0.0	0.0	26.29018250246283	0.0
36	1.6759643582715713E-4	0.0	0.0	26.852602621811602	0.0
37	1.6759643582715713E-4	0.0	0.0	27.404229530693108	3.3519287165431426E-4
38	1.6759643582715713E-4	0.0	0.0	27.97305183389048	3.3519287165431426E-4
39	1.6759643582715713E-4	0.0	0.0	28.55565056411284	3.3519287165431426E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGT	20	7.035099E-4	45.0	37
CGTACGA	30	2.166671E-6	44.999996	32
CGTTTTT	19060	0.0	43.890347	1
CGTAAGG	405	0.0	38.333332	2
TTATCCG	65	9.094947E-12	38.076927	1
CGGTCTA	90	0.0	37.5	31
CGTACCG	30	1.14035865E-4	37.499996	2
AACTCGC	30	1.14035865E-4	37.499996	38
GCGTAAC	60	1.5643309E-10	37.499996	32
TAGCACG	145	0.0	37.241383	1
GTTTTTT	23035	0.0	36.541134	2
TGACGTA	25	0.0021076463	36.0	32
CGGTCGA	25	0.0021076463	36.0	18
CGACTAT	25	0.0021076463	36.0	37
CGTTTCT	1175	0.0	35.425533	1
CGTTCTG	970	0.0	35.257732	1
TAGGGCG	1025	0.0	35.121952	5
CGAGGGA	1755	0.0	34.358974	4
GCGTAAG	210	0.0	34.285717	1
TGGGCGA	1725	0.0	34.173912	6
>>END_MODULE