FastQCFastQC Report
Sat 14 Jan 2017
SRR2934730.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934730.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences762292
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC56660.7432847255382452TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC48530.6366326814396582TruSeq Adapter, Index 22 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGCT38850.5096472217995204TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29280.38410477874620225No Hit
TCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC27120.35576918031410537TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGC21120.2770591846693918No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTC19440.255020385888872No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCG18440.24190205328141973No Hit
ACTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC16290.21369763817539736TruSeq Adapter, Index 20 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTG14370.188510439569089No Hit
ACCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTG11400.1495489917249558No Hit
GCCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTG9830.12895320953125575TruSeq Adapter, Index 22 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTG8920.11701552685847419No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCC8780.11517896029343086No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCG302.1645355E-645.0000041
GGTACGA302.1645355E-645.00000414
TCACCGC302.1645355E-645.00000442
GCGGTCC302.1645355E-645.00000421
TCCGAGC302.1645355E-645.00000423
ACCGCGT302.1645355E-645.00000444
TAGCGCG600.045.0000041
CAATTCG302.1645355E-645.0000041
TCTAGCG1150.045.0000041
ATCGTAG302.1645355E-645.0000041
GCCGATC302.1645355E-645.0000049
TCGTGCG302.1645355E-645.0000041
AGGTTCG302.1645355E-645.0000041
ATCCAAC302.1645355E-645.00000414
CCGCGTT302.1645355E-645.00000445
AGTACGT302.1645355E-645.00000410
TCCGGAA253.8895647E-545.045
TCACGCC207.0317904E-445.012
TCACGAC207.0317904E-445.025
CTTCGTT207.0317904E-445.031
Produced by FastQC (version 0.11.5)