##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934730.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	762292
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.094660576262115	33.0	31.0	34.0	30.0	34.0
2	32.28264365886039	34.0	31.0	34.0	30.0	34.0
3	32.294988009844	34.0	31.0	34.0	30.0	34.0
4	35.859131408961396	37.0	35.0	37.0	35.0	37.0
5	35.739758517733364	37.0	35.0	37.0	35.0	37.0
6	35.6714566071794	37.0	35.0	37.0	33.0	37.0
7	36.00265121501997	37.0	35.0	37.0	35.0	37.0
8	35.87262361404816	37.0	35.0	37.0	35.0	37.0
9	37.654654646775775	39.0	38.0	39.0	35.0	39.0
10	37.29596532562325	39.0	37.0	39.0	34.0	39.0
11	37.181484260624536	39.0	37.0	39.0	34.0	39.0
12	37.00634140198244	39.0	35.0	39.0	33.0	39.0
13	36.83516421528758	39.0	35.0	39.0	33.0	39.0
14	37.909138492861004	40.0	37.0	41.0	33.0	41.0
15	38.02116905332865	40.0	37.0	41.0	33.0	41.0
16	38.142573974277575	40.0	37.0	41.0	34.0	41.0
17	38.17476373882974	40.0	37.0	41.0	34.0	41.0
18	38.133514453778865	40.0	37.0	41.0	34.0	41.0
19	38.09899618518888	40.0	37.0	41.0	34.0	41.0
20	38.019144894607315	40.0	36.0	41.0	34.0	41.0
21	37.945585156344286	40.0	36.0	41.0	34.0	41.0
22	37.90260031588945	40.0	36.0	41.0	34.0	41.0
23	37.726457840302665	39.0	35.0	41.0	33.0	41.0
24	37.47412015343202	39.0	35.0	41.0	33.0	41.0
25	37.4075144432842	39.0	35.0	41.0	33.0	41.0
26	37.56552082404118	39.0	35.0	41.0	33.0	41.0
27	37.636254873460565	39.0	35.0	41.0	33.0	41.0
28	37.59057815115467	39.0	35.0	41.0	33.0	41.0
29	37.570576629428096	39.0	36.0	41.0	33.0	41.0
30	37.50191921206047	39.0	36.0	41.0	33.0	41.0
31	37.31190803524109	39.0	35.0	41.0	33.0	41.0
32	37.200475670740346	39.0	35.0	41.0	33.0	41.0
33	37.10745750972068	39.0	35.0	41.0	32.0	41.0
34	37.006835963121745	39.0	35.0	41.0	32.0	41.0
35	36.96878492756057	39.0	35.0	41.0	32.0	41.0
36	36.89384120520745	39.0	35.0	41.0	32.0	41.0
37	36.80356739936927	39.0	35.0	41.0	31.0	41.0
38	36.730405146584246	39.0	35.0	41.0	31.0	41.0
39	36.80148945548425	39.0	35.0	41.0	31.0	41.0
40	36.73782749917355	39.0	35.0	41.0	31.0	41.0
41	36.72033682630803	39.0	35.0	41.0	31.0	41.0
42	36.63193763019945	39.0	35.0	41.0	31.0	41.0
43	36.583130873733424	39.0	35.0	41.0	31.0	41.0
44	36.5071048889402	39.0	35.0	41.0	31.0	41.0
45	36.47695240144197	39.0	35.0	40.0	31.0	41.0
46	36.424952642819285	39.0	35.0	40.0	31.0	41.0
47	36.294454093706875	39.0	35.0	40.0	30.0	41.0
48	36.30525441694259	39.0	35.0	40.0	31.0	41.0
49	36.33679875953047	39.0	35.0	40.0	31.0	41.0
50	36.247561301968275	39.0	35.0	40.0	31.0	41.0
51	35.281267545769865	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	5.0
11	6.0
12	10.0
13	7.0
14	8.0
15	14.0
16	27.0
17	80.0
18	135.0
19	294.0
20	501.0
21	931.0
22	1341.0
23	2072.0
24	2901.0
25	4305.0
26	6004.0
27	6982.0
28	7660.0
29	8786.0
30	10856.0
31	13803.0
32	18490.0
33	26545.0
34	49983.0
35	74351.0
36	56883.0
37	89851.0
38	166691.0
39	212636.0
40	127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.52144847381319	22.02108903149974	27.881441757226888	12.576020737460187
2	29.632345610343542	24.971008484937528	27.090799850975745	18.305846053743185
3	30.289705257302973	23.35561700765586	29.949678076117813	16.40499965892335
4	27.22303264365886	24.924700770833226	29.95151464268286	17.90075194282506
5	24.30420363850073	28.269613219081403	27.873439574336345	19.552743568081524
6	23.217874515277607	32.51063896774464	27.466246530201026	16.80523998677672
7	76.34987642530685	5.7571114481064996	11.787477764426232	6.105534362160432
8	77.89508482313863	3.8979813509783656	10.728303589700534	7.478630236182459
9	73.34184275841803	4.736636354572788	13.513850335566948	8.40767055144223
10	39.57289332696657	22.550807302188662	21.393507999559226	16.482791371285543
11	28.819402538659727	25.12068865998856	26.84050206482555	19.21940673652616
12	27.042130837002095	21.40059189916725	32.070650092090695	19.48662717173996
13	21.71674371500685	26.893631311885734	33.43089000015742	17.95873497295
14	17.473356666474267	31.077461130380485	29.879232630015796	21.569949573129456
15	14.391860336983727	25.107439144055032	40.5680500385679	19.93265048039334
16	16.294149748390378	24.8563542579484	35.74784990528564	23.10164608837558
17	17.040320507102265	24.31601013784744	30.260844925566584	28.382824429483716
18	18.137537846389574	25.341601381098055	34.81644829015653	21.704412482355842
19	19.80068005436237	27.211750877616453	30.354772187035937	22.63279688098524
20	22.94750568023802	26.96722515781354	29.8019656509579	20.28330351099054
21	19.33563516342819	28.4488096424992	31.55536198726997	20.660193206802642
22	18.129010930194728	25.66824786302362	28.98075802973139	27.221983177050262
23	17.634843340872003	27.272751124241108	29.25296343133602	25.83944210355087
24	20.25050767947191	23.50096813294643	31.917821517213874	24.330702670367785
25	16.7425343569131	27.34975573664685	29.90455101194818	26.003158894491875
26	17.677084371868	29.189208334863803	29.845518515214643	23.288188778053552
27	20.914426492735068	28.694384828910707	29.36473162515152	21.02645705320271
28	15.728224879704891	26.69567567283928	34.174174725695664	23.401924721760164
29	17.59771845959291	27.761802563846928	31.31201691740173	23.328462059158433
30	23.745625036075417	26.542322364658162	28.64584699826313	21.06620560100329
31	20.847129446458837	28.51322065560179	28.09605767868481	22.543592219254563
32	21.450441563075568	30.662659453332843	25.173555540396592	22.713343443194997
33	23.388806389152712	25.935861848215648	26.477386618251273	24.19794514438037
34	18.191060643427978	25.718491076910162	31.58527178561496	24.5051764940469
35	20.023298158710833	25.78106552344771	26.788159917721817	27.40747640011964
36	23.53232094787824	24.508587260524838	28.64715883152388	23.311932960073044
37	19.881357799898204	25.451401825022437	32.97004297565762	21.697197399421743
38	18.813131975673365	27.537610259585566	27.053806153022723	26.595451611718346
39	21.86091419036275	24.02596380389667	29.881331563232987	24.231790442507595
40	22.737349991866633	22.172343406463664	30.742681282238305	24.347625319431398
41	18.193946676601616	23.352599791156145	27.904792389268156	30.548661142974083
42	20.74192041894707	22.959180996258652	28.968164430428235	27.33073415436604
43	23.28071132846731	23.357584757546977	28.63705771541614	24.724646198569577
44	21.313486170653764	24.409281482686424	28.711176294648244	25.566056052011565
45	21.606418537778175	24.05534886893736	27.83906954290482	26.499163050379643
46	25.114260677010908	23.235321897645523	28.76181305851301	22.88860436683056
47	18.37301191669334	23.16684420143462	32.8059326347384	25.654211247133645
48	19.666217145135985	21.14019299690932	30.58985270736148	28.60373715059321
49	21.150818846321357	18.544599707198817	34.21221789025728	26.092363556222548
50	20.16038473445871	20.0126723092988	32.12876430554171	27.698178650700783
51	19.0163349477628	20.313869225965902	27.85651692527273	32.81327890099857
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	214.0
1	307.5
2	401.0
3	2578.5
4	4756.0
5	3190.0
6	1624.0
7	1747.5
8	1871.0
9	1966.0
10	2061.0
11	2071.0
12	2081.0
13	2044.5
14	2008.0
15	1972.0
16	1936.0
17	1981.5
18	2027.0
19	1812.5
20	1598.0
21	1663.5
22	1729.0
23	1893.0
24	2057.0
25	3226.0
26	4755.0
27	5115.0
28	5837.5
29	6560.0
30	7397.0
31	8234.0
32	9870.5
33	11507.0
34	13309.0
35	15111.0
36	17126.0
37	19141.0
38	21980.5
39	24820.0
40	27317.0
41	29814.0
42	37310.0
43	44806.0
44	55020.5
45	65235.0
46	80989.5
47	96744.0
48	104648.5
49	112553.0
50	108124.5
51	103696.0
52	83976.5
53	64257.0
54	51237.5
55	38218.0
56	32118.0
57	26018.0
58	22310.5
59	18603.0
60	16913.0
61	15223.0
62	13814.5
63	12406.0
64	9859.5
65	7313.0
66	5412.0
67	3511.0
68	2860.0
69	2209.0
70	1542.0
71	875.0
72	709.0
73	543.0
74	513.5
75	348.5
76	213.0
77	209.5
78	206.0
79	153.5
80	101.0
81	57.0
82	13.0
83	8.0
84	3.0
85	2.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	762292.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.23867020608127
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.83908436236369	16.6169093689206
2	12.637226993586292	6.3789360882102475
3	6.086719774292751	4.6086213906062445
4	3.4817339796952833	3.5149734263534445
5	2.1898365972368765	2.763428184143437
6	1.5621807233877578	2.3656418447928655
7	1.1023323276811228	1.9474981453092037
8	0.7905119906830621	1.5961177141442078
9	0.6034557765614134	1.3707379195729361
>10	4.21743461379762	23.161299840233845
>50	1.2348479691224918	22.016629308309014
>100	0.24413448369097107	8.827296495580054
>500	0.004725183555309117	0.8367890683997785
>1k	0.005250203950343464	3.244329936769624
>5k	5.250203950343463E-4	0.7507912686544964
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC	5666	0.7432847255382452	TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC	4853	0.6366326814396582	TruSeq Adapter, Index 22 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGCT	3885	0.5096472217995204	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2928	0.38410477874620225	No Hit
TCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC	2712	0.35576918031410537	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGC	2112	0.2770591846693918	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTC	1944	0.255020385888872	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCG	1844	0.24190205328141973	No Hit
ACTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC	1629	0.21369763817539736	TruSeq Adapter, Index 20 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTG	1437	0.188510439569089	No Hit
ACCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTG	1140	0.1495489917249558	No Hit
GCCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTG	983	0.12895320953125575	TruSeq Adapter, Index 22 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTG	892	0.11701552685847419	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCC	878	0.11517896029343086	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.536670986970872	0.0
2	0.0	0.0	0.0	2.6049073058617958	0.0
3	0.0	0.0	0.0	3.6644750305657148	0.0
4	1.3118332607452263E-4	0.0	0.0	4.842632482041003	0.0
5	1.3118332607452263E-4	0.0	0.0	7.8509285155819555	0.0
6	1.3118332607452263E-4	0.0	0.0	9.358618482156444	0.0
7	1.3118332607452263E-4	0.0	0.0	11.120148184685133	0.0
8	1.3118332607452263E-4	0.0	0.0	13.786711653801955	0.0
9	1.3118332607452263E-4	0.0	0.0	14.743694017515597	0.0
10	1.3118332607452263E-4	0.0	0.0	17.065245339056425	0.0
11	1.3118332607452263E-4	0.0	0.0	20.146610485220886	0.0
12	1.3118332607452263E-4	0.0	0.0	22.725805859172077	0.0
13	1.3118332607452263E-4	0.0	0.0	23.643170858411214	0.0
14	1.3118332607452263E-4	0.0	0.0	23.963520540685195	0.0
15	1.3118332607452263E-4	0.0	0.0	24.556469174542038	0.0
16	1.3118332607452263E-4	0.0	0.0	25.93953498134573	0.0
17	1.3118332607452263E-4	0.0	0.0	27.656199986356935	0.0
18	1.3118332607452263E-4	0.0	0.0	29.71171152261863	0.0
19	1.3118332607452263E-4	0.0	0.0	30.753175948324266	0.0
20	1.3118332607452263E-4	0.0	0.0	31.684839930105525	0.0
21	1.3118332607452263E-4	0.0	0.0	32.92386644487939	0.0
22	1.3118332607452263E-4	0.0	0.0	34.10687767941943	0.0
23	1.3118332607452263E-4	0.0	0.0	35.310353512827106	0.0
24	1.3118332607452263E-4	0.0	0.0	36.24162394463014	0.0
25	1.3118332607452263E-4	0.0	0.0	37.03869383385894	0.0
26	1.3118332607452263E-4	0.0	0.0	37.708909446773674	0.0
27	1.3118332607452263E-4	0.0	0.0	38.40864130805518	0.0
28	1.3118332607452263E-4	0.0	0.0	39.04934067260315	0.0
29	1.3118332607452263E-4	0.0	0.0	39.73319935142963	0.0
30	1.3118332607452263E-4	0.0	0.0	40.48658519307562	0.0
31	1.3118332607452263E-4	0.0	0.0	41.231575301852835	0.0
32	1.3118332607452263E-4	0.0	0.0	41.920418947070154	0.0
33	1.3118332607452263E-4	0.0	0.0	42.525698813577996	0.0
34	1.3118332607452263E-4	0.0	0.0	43.13832494634602	0.0
35	1.3118332607452263E-4	0.0	0.0	43.83936864088827	0.0
36	1.3118332607452263E-4	0.0	0.0	44.482560488631655	0.0
37	1.3118332607452263E-4	0.0	0.0	45.101614604377325	0.0
38	1.3118332607452263E-4	0.0	0.0	45.67698467254018	0.0
39	1.3118332607452263E-4	0.0	0.0	46.24513965776894	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGCG	30	2.1645355E-6	45.000004	1
GGTACGA	30	2.1645355E-6	45.000004	14
TCACCGC	30	2.1645355E-6	45.000004	42
GCGGTCC	30	2.1645355E-6	45.000004	21
TCCGAGC	30	2.1645355E-6	45.000004	23
ACCGCGT	30	2.1645355E-6	45.000004	44
TAGCGCG	60	0.0	45.000004	1
CAATTCG	30	2.1645355E-6	45.000004	1
TCTAGCG	115	0.0	45.000004	1
ATCGTAG	30	2.1645355E-6	45.000004	1
GCCGATC	30	2.1645355E-6	45.000004	9
TCGTGCG	30	2.1645355E-6	45.000004	1
AGGTTCG	30	2.1645355E-6	45.000004	1
ATCCAAC	30	2.1645355E-6	45.000004	14
CCGCGTT	30	2.1645355E-6	45.000004	45
AGTACGT	30	2.1645355E-6	45.000004	10
TCCGGAA	25	3.8895647E-5	45.0	45
TCACGCC	20	7.0317904E-4	45.0	12
TCACGAC	20	7.0317904E-4	45.0	25
CTTCGTT	20	7.0317904E-4	45.0	31
>>END_MODULE