SRR2934729.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934729.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1041094
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	4743	0.4555784588135174	RNA PCR Primer, Index 23 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	4508	0.4330060494057213	RNA PCR Primer, Index 1 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGC	3666	0.35212958676161804	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3019	0.289983421285686	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCG	2924	0.28085840471657697	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTC	2625	0.2521386157253812	No Hit
CTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGCT	2421	0.23254384330329442	Illumina Paired End PCR Primer 2 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	1829	0.17568058215684657	RNA PCR Primer, Index 23 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	1197	0.11497520877077382	RNA PCR Primer, Index 23 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCTTCG	30	2.1653032E-6	45.000004	24
GTCCGTT	30	2.1653032E-6	45.000004	11
TTACGGA	30	2.1653032E-6	45.000004	22
ATTTACG	20	7.03298E-4	45.0	20
TATCACG	20	7.03298E-4	45.0	1
TTCTACG	35	1.2117744E-7	45.0	1
AGCGTAA	25	3.8905513E-5	45.0	15
AACCGGC	20	7.03298E-4	45.0	45
GTACCGT	20	7.03298E-4	45.0	42
ACACGTA	20	7.03298E-4	45.0	39
GTCGATT	25	3.8905513E-5	45.0	16
GTCGACT	20	7.03298E-4	45.0	9
TTTACGT	20	7.03298E-4	45.0	16
CTATCCG	25	3.8905513E-5	45.0	18
TAGACGT	25	3.8905513E-5	45.0	22
CTATACG	25	3.8905513E-5	45.0	1
CGGCACG	20	7.03298E-4	45.0	34
ATTACCG	25	3.8905513E-5	45.0	1
GCGCCTA	20	7.03298E-4	45.0	15
ATTCGAC	25	3.8905513E-5	45.0	12