SRR2934728.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934728.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1084723
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGC	36635	3.377359934287371	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCG	32385	2.9855548375022933	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC	28342	2.612832953666512	No Hit
GCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	10188	0.9392259590697348	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCT	7000	0.6453260417636576	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATGGCGAT	6804	0.6272569125942752	No Hit
CCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	5388	0.4967166732889411	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAATGGCGATCGTA	4750	0.4378998140539106	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTC	4536	0.4181712750628502	No Hit
GAACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCT	4142	0.38184863785501	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3856	0.3554824595772377	No Hit
CTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCT	3768	0.3473697893379231	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	2370	0.21848895985426697	No Hit
CGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG	1946	0.1794006396102968	No Hit
ACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	1809	0.16677068707863668	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATG	1590	0.14658120091488794	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAATGGCGA	1376	0.12685266192382755	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTAT	1282	0.11818685507728702	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTT	1229	0.11330081504679075	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAATGGCGATCGT	1192	0.10988980596889712	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGC	1164	0.1073085018018425	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTTCG	35	1.2118471E-7	45.000004	29
TAGAACG	35	1.2118471E-7	45.000004	1
TTGTCCG	35	1.2118471E-7	45.000004	24
ACTACGG	35	1.2118471E-7	45.000004	2
ATACGTA	35	1.2118471E-7	45.000004	29
TTCGCAG	90	0.0	45.000004	1
TGCAACC	35	1.2118471E-7	45.000004	30
CTTAACG	20	7.0331106E-4	45.0	11
AAGTACG	25	3.8906604E-5	45.0	1
TCGTTAA	20	7.0331106E-4	45.0	43
CTCGTTA	20	7.0331106E-4	45.0	42
TCACCGC	20	7.0331106E-4	45.0	19
CATATCG	20	7.0331106E-4	45.0	12
GTCGACA	25	3.8906604E-5	45.0	9
CGAGTAT	20	7.0331106E-4	45.0	33
CGTGCTC	25	3.8906604E-5	45.0	11
CAACGCG	20	7.0331106E-4	45.0	1
GCGGTAA	20	7.0331106E-4	45.0	37
GACGTCC	25	3.8906604E-5	45.0	34
CCGTGTA	25	3.8906604E-5	45.0	15

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content