##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934728.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1084723
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.028361157641164	33.0	31.0	34.0	30.0	34.0
2	32.201857985863676	33.0	31.0	34.0	30.0	34.0
3	32.231047926521335	34.0	31.0	34.0	30.0	34.0
4	35.83501502226836	37.0	35.0	37.0	35.0	37.0
5	35.70202991915909	37.0	35.0	37.0	35.0	37.0
6	35.57467390292268	37.0	35.0	37.0	33.0	37.0
7	35.886134063719496	37.0	35.0	37.0	35.0	37.0
8	35.403167444591844	37.0	35.0	37.0	33.0	37.0
9	37.32848201798985	39.0	37.0	39.0	34.0	39.0
10	37.155414792532284	39.0	37.0	39.0	34.0	39.0
11	37.048728569413576	39.0	37.0	39.0	33.0	39.0
12	36.83592032251552	39.0	35.0	39.0	33.0	39.0
13	36.57519016375609	39.0	35.0	39.0	32.0	39.0
14	37.607084020528745	39.0	36.0	41.0	33.0	41.0
15	37.58257638125125	39.0	36.0	41.0	33.0	41.0
16	37.893419794731	40.0	36.0	41.0	33.0	41.0
17	37.73648940789492	39.0	36.0	41.0	33.0	41.0
18	37.769471099995116	39.0	36.0	41.0	33.0	41.0
19	37.724094538421326	39.0	36.0	41.0	33.0	41.0
20	37.50898155565983	39.0	35.0	41.0	32.0	41.0
21	37.61167966384045	39.0	35.0	41.0	33.0	41.0
22	37.525269584953946	39.0	35.0	41.0	33.0	41.0
23	37.24003363070572	39.0	35.0	41.0	32.0	41.0
24	36.99745557160676	39.0	35.0	41.0	32.0	41.0
25	36.989203695321294	39.0	35.0	40.0	32.0	41.0
26	37.12276221671339	39.0	35.0	41.0	32.0	41.0
27	37.118202527281156	39.0	35.0	41.0	32.0	41.0
28	37.10729559528101	39.0	35.0	41.0	32.0	41.0
29	37.07276696446927	39.0	35.0	41.0	32.0	41.0
30	36.97243904665062	39.0	35.0	41.0	32.0	41.0
31	36.9402289801175	39.0	35.0	41.0	32.0	41.0
32	36.71647508165679	39.0	35.0	40.0	31.0	41.0
33	36.56795421503923	39.0	35.0	40.0	31.0	41.0
34	36.43038637513909	39.0	35.0	40.0	30.0	41.0
35	36.37668234194352	39.0	35.0	41.0	30.0	41.0
36	36.25219894848731	39.0	35.0	40.0	30.0	41.0
37	36.11704370608902	39.0	35.0	40.0	30.0	41.0
38	36.04090906157609	38.0	35.0	40.0	30.0	41.0
39	36.10236807000497	39.0	35.0	40.0	30.0	41.0
40	36.02630072377925	38.0	35.0	40.0	30.0	41.0
41	35.921537572264995	38.0	35.0	40.0	29.0	41.0
42	35.91692164727769	38.0	35.0	40.0	30.0	41.0
43	35.832238276500085	38.0	35.0	40.0	29.0	41.0
44	35.90994475087188	38.0	35.0	40.0	30.0	41.0
45	35.85952266154585	38.0	35.0	40.0	29.0	41.0
46	35.7674309478088	38.0	35.0	40.0	29.0	41.0
47	35.454900467677	38.0	35.0	40.0	28.0	41.0
48	35.50664270970561	38.0	35.0	40.0	28.0	41.0
49	35.6076703453324	38.0	35.0	40.0	28.0	41.0
50	35.47908175635623	38.0	35.0	40.0	28.0	41.0
51	34.48987621724625	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	12.0
10	17.0
11	16.0
12	10.0
13	14.0
14	20.0
15	25.0
16	77.0
17	127.0
18	296.0
19	674.0
20	1402.0
21	2680.0
22	4223.0
23	5507.0
24	7062.0
25	8942.0
26	10787.0
27	11753.0
28	12798.0
29	14550.0
30	18184.0
31	23907.0
32	32454.0
33	44612.0
34	77020.0
35	110181.0
36	87711.0
37	128048.0
38	217875.0
39	263562.0
40	168.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.343749510243626	18.58262431975721	20.41811596140213	11.655510208597034
2	27.761280990630787	36.476040426910835	20.767237349996268	14.995441232462111
3	25.762521860419664	35.30763153358046	24.205995447685723	14.72385115831415
4	23.396756591314098	22.50851138954369	37.82652345345309	16.26820856568912
5	32.32650178893598	25.331536253956077	23.50010094743082	18.841861009677125
6	22.663942776174192	38.72601576623709	25.08778738903849	13.522254068550218
7	68.1583224472976	4.913512481988489	21.407308593991278	5.520856476722629
8	65.58374810896423	14.397131802312666	12.597962797875587	7.421157290847526
9	61.87736408281192	5.473378917935731	12.795432566655265	19.85382443259708
10	43.88696469052468	17.764258709366352	22.127123698861368	16.221652901247598
11	34.55877675683101	20.475734357988166	24.61494777929481	20.35054110588602
12	25.956857188425065	18.440468211700132	30.849534858208038	24.753139741666764
13	22.985315144972496	23.892182612519512	37.42642130755963	15.69608093494837
14	18.91542817843818	32.23634052195814	27.483329845499725	21.36490145410395
15	14.510524806793992	21.873326185579174	44.35067754624914	19.265471461377697
16	15.939092284389655	24.002533365661094	30.484833455177036	29.573540894772215
17	16.79857438258431	24.22019262060452	32.63164881725565	26.349584179555517
18	17.265882626255735	24.00778816342974	33.26775591556554	25.45857329474898
19	18.522240240135037	25.664893249244276	29.492045434640918	26.320821075979765
20	21.31244566585202	25.668949584363936	33.73303599167714	19.28556875810691
21	18.96852929273188	32.21172594293659	29.619819990910123	19.19992477342142
22	17.017985236783954	23.6792250187375	30.69843637500081	28.604353369477735
23	18.166849969992338	29.76603243408686	29.365561530455242	22.70155606546556
24	20.90257143989756	23.324203506332953	28.367149954412323	27.406075099357164
25	15.781909298502935	33.749076953286696	27.03473605703945	23.434277691170927
26	16.2975248058721	26.34064180440536	30.967629523850785	26.394203865871745
27	21.368957789223607	30.19803212433036	25.684898356538948	22.748111729907084
28	14.66586400398996	26.278690504396053	36.022284030116445	23.033161461497546
29	21.45819716185607	25.804652432003376	27.984748179950085	24.752402226190465
30	19.197988795296126	32.98685470852927	28.607672189121093	19.207484307053505
31	22.639696954890788	24.39406189414256	26.480216608295386	26.48602454267126
32	29.655958249248886	29.324352853216908	22.167687050057943	18.85200184747627
33	24.467352494600004	25.2951214273137	22.862795386471937	27.374730691614356
34	23.294334129542747	26.446475275254606	25.181636233397835	25.07755436180481
35	24.76964165044901	27.140846096192302	22.848690402987675	25.240821850371013
36	21.99427872369259	32.411500447579705	25.876560190942755	19.717660637784945
37	23.31848776139162	27.06810863234208	29.9974279147764	19.6159756914899
38	25.487612966628348	25.053769487694094	25.53002010651567	23.928597439161887
39	27.81604151474616	23.970728010745603	25.088248336211176	23.12498213829706
40	28.12524487818549	21.330146037283253	26.655376533916954	23.889232550614302
41	19.040805809409406	28.55669143182176	22.84030116444475	29.562201594324083
42	24.280484510792157	23.082390619540657	30.463076748626143	22.17404812104104
43	24.04429517950666	27.875780268326565	24.72686575282353	23.353058799343245
44	22.881509841683084	23.142129373121065	28.665751532879824	25.31060925231603
45	21.17775690199249	21.379743953064516	27.17643121792384	30.266067927019158
46	29.657709848505103	25.047684985014605	24.40429492137624	20.89031024510405
47	18.56639897927858	22.51985068999182	36.779620234843364	22.13413009588623
48	22.311594757371235	23.73426211115649	26.821778463257438	27.132364668214837
49	21.884204538854622	18.546486061418445	35.685608215184885	23.883701184542044
50	23.416208562001543	20.137491322669472	29.693940296278402	26.752359819050575
51	21.203846511966653	20.196215992469966	24.96038159050744	33.63955590505594
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	290.0
1	479.5
2	669.0
3	3589.5
4	6510.0
5	4528.5
6	2547.0
7	2507.0
8	2467.0
9	2687.5
10	2908.0
11	3030.0
12	3152.0
13	3073.5
14	2995.0
15	2866.5
16	2738.0
17	2633.0
18	2528.0
19	2631.5
20	2735.0
21	2699.0
22	2663.0
23	2983.5
24	3304.0
25	3374.0
26	4523.0
27	5602.0
28	6174.0
29	6746.0
30	8230.5
31	9715.0
32	11137.0
33	12559.0
34	14226.5
35	15894.0
36	19306.0
37	22718.0
38	25251.0
39	27784.0
40	33265.5
41	38747.0
42	49603.0
43	60459.0
44	67715.5
45	74972.0
46	146984.5
47	218997.0
48	179512.0
49	140027.0
50	131751.0
51	123475.0
52	102330.0
53	81185.0
54	69410.0
55	57635.0
56	49283.0
57	40931.0
58	35518.0
59	30105.0
60	26987.0
61	23869.0
62	20712.0
63	17555.0
64	14579.5
65	11604.0
66	9713.0
67	7822.0
68	7043.5
69	6265.0
70	5061.0
71	3857.0
72	3602.0
73	3347.0
74	2560.0
75	1378.0
76	983.0
77	730.0
78	477.0
79	338.0
80	199.0
81	236.0
82	273.0
83	191.5
84	110.0
85	87.0
86	64.0
87	38.5
88	13.0
89	10.0
90	7.0
91	5.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1084723.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.17393501347427
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.02323239091788	15.083427344426712
2	12.192848307273755	5.40726851989276
3	5.591970810716684	3.7198799206223034
4	3.22016353814206	2.8561478811008567
5	2.076817834007896	2.3025611843057745
6	1.425857817935437	1.8970127156012768
7	0.9498075528553026	1.4742679666624356
8	0.7090615461014962	1.2578147715046535
9	0.5311660619492945	1.0600237565124035
>10	3.6312511200092197	16.969962720011505
>50	1.2196320402979168	19.665505443324662
>100	0.4131079315812182	12.244698449248066
>500	0.005865629860179569	0.9361827776198418
>1k	0.006284603421620966	3.3505439986959997
>5k	0.0012569206843241931	1.78299294116106
>10k+	0.001675894245765591	9.991709609309712
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGC	36635	3.377359934287371	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCG	32385	2.9855548375022933	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC	28342	2.612832953666512	No Hit
GCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	10188	0.9392259590697348	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCT	7000	0.6453260417636576	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATGGCGAT	6804	0.6272569125942752	No Hit
CCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	5388	0.4967166732889411	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAATGGCGATCGTA	4750	0.4378998140539106	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTC	4536	0.4181712750628502	No Hit
GAACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCT	4142	0.38184863785501	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3856	0.3554824595772377	No Hit
CTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCT	3768	0.3473697893379231	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	2370	0.21848895985426697	No Hit
CGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG	1946	0.1794006396102968	No Hit
ACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	1809	0.16677068707863668	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATG	1590	0.14658120091488794	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAATGGCGA	1376	0.12685266192382755	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTAT	1282	0.11818685507728702	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTT	1229	0.11330081504679075	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAATGGCGATCGT	1192	0.10988980596889712	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGC	1164	0.1073085018018425	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.37023276910326414	0.0
2	0.0	0.0	0.0	2.310359418948432	0.0
3	0.0	0.0	0.0	3.085395995106585	0.0
4	0.0	0.0	0.0	4.315018672969965	0.0
5	0.0	0.0	0.0	9.108961458363103	0.0
6	9.218943453766538E-5	0.0	0.0	10.277185972824398	0.0
7	9.218943453766538E-5	0.0	0.0	12.157389490220083	0.0
8	9.218943453766538E-5	0.0	0.0	14.275902695895635	0.0
9	9.218943453766538E-5	0.0	0.0	14.916250508194258	0.0
10	9.218943453766538E-5	0.0	0.0	19.515489207843846	0.0
11	9.218943453766538E-5	0.0	0.0	21.635108686733847	0.0
12	9.218943453766538E-5	0.0	0.0	26.89488468484581	0.0
13	9.218943453766538E-5	0.0	0.0	27.74265872485418	0.0
14	9.218943453766538E-5	0.0	0.0	28.119252564940542	0.0
15	9.218943453766538E-5	0.0	0.0	29.0837384290736	0.0
16	9.218943453766538E-5	0.0	0.0	30.204946331920684	0.0
17	9.218943453766538E-5	0.0	0.0	31.46987756321199	0.0
18	9.218943453766538E-5	0.0	0.0	32.74347460134984	0.0
19	9.218943453766538E-5	0.0	0.0	34.260728314970734	0.0
20	9.218943453766538E-5	0.0	0.0	35.10186471569239	0.0
21	9.218943453766538E-5	0.0	0.0	35.96033273010713	0.0
22	9.218943453766538E-5	0.0	0.0	36.86185321045096	0.0
23	9.218943453766538E-5	0.0	0.0	37.76807535195621	0.0
24	9.218943453766538E-5	0.0	0.0	38.48899673004076	0.0
25	9.218943453766538E-5	0.0	0.0	39.10869410900295	0.0
26	9.218943453766538E-5	0.0	0.0	39.674368479326056	0.0
27	9.218943453766538E-5	0.0	0.0	40.25995576750931	0.0
28	9.218943453766538E-5	0.0	0.0	40.77547908544393	0.0
29	9.218943453766538E-5	0.0	0.0	41.34585511692847	0.0
30	9.218943453766538E-5	0.0	0.0	41.977813690684165	0.0
31	1.8437886907533076E-4	0.0	0.0	42.59446881830661	0.0
32	1.8437886907533076E-4	0.0	0.0	43.157469695028134	0.0
33	1.8437886907533076E-4	0.0	0.0	43.670319519361165	0.0
34	1.8437886907533076E-4	0.0	0.0	44.197366516612995	0.0
35	1.8437886907533076E-4	0.0	0.0	44.734738730533046	0.0
36	1.8437886907533076E-4	0.0	0.0	45.27367816484024	0.0
37	1.8437886907533076E-4	0.0	0.0	45.79058432429293	0.0
38	1.8437886907533076E-4	0.0	0.0	46.28259933642045	0.0
39	1.8437886907533076E-4	0.0	0.0	46.77111115003554	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTTCG	35	1.2118471E-7	45.000004	29
TAGAACG	35	1.2118471E-7	45.000004	1
TTGTCCG	35	1.2118471E-7	45.000004	24
ACTACGG	35	1.2118471E-7	45.000004	2
ATACGTA	35	1.2118471E-7	45.000004	29
TTCGCAG	90	0.0	45.000004	1
TGCAACC	35	1.2118471E-7	45.000004	30
CTTAACG	20	7.0331106E-4	45.0	11
AAGTACG	25	3.8906604E-5	45.0	1
TCGTTAA	20	7.0331106E-4	45.0	43
CTCGTTA	20	7.0331106E-4	45.0	42
TCACCGC	20	7.0331106E-4	45.0	19
CATATCG	20	7.0331106E-4	45.0	12
GTCGACA	25	3.8906604E-5	45.0	9
CGAGTAT	20	7.0331106E-4	45.0	33
CGTGCTC	25	3.8906604E-5	45.0	11
CAACGCG	20	7.0331106E-4	45.0	1
GCGGTAA	20	7.0331106E-4	45.0	37
GACGTCC	25	3.8906604E-5	45.0	34
CCGTGTA	25	3.8906604E-5	45.0	15
>>END_MODULE