##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934725.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	958726
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1053898611282	33.0	31.0	34.0	30.0	34.0
2	32.28704968885792	34.0	31.0	34.0	30.0	34.0
3	32.28563113965826	34.0	31.0	34.0	30.0	34.0
4	35.87264453034548	37.0	35.0	37.0	35.0	37.0
5	35.749204673702394	37.0	35.0	37.0	35.0	37.0
6	35.65847280662045	37.0	35.0	37.0	33.0	37.0
7	35.98858276504444	37.0	35.0	37.0	35.0	37.0
8	35.79493932573019	37.0	35.0	37.0	35.0	37.0
9	37.468168173179826	39.0	37.0	39.0	35.0	39.0
10	37.266598590212425	39.0	37.0	39.0	34.0	39.0
11	37.10195509457343	39.0	37.0	39.0	34.0	39.0
12	36.87012660551607	39.0	35.0	39.0	33.0	39.0
13	36.67575303058434	39.0	35.0	39.0	33.0	39.0
14	37.67187705350642	40.0	36.0	41.0	33.0	41.0
15	37.809155066202436	40.0	36.0	41.0	33.0	41.0
16	37.954710730698864	40.0	36.0	41.0	33.0	41.0
17	37.96539887308783	40.0	36.0	41.0	34.0	41.0
18	37.93934241900188	40.0	36.0	41.0	34.0	41.0
19	37.89795833220336	40.0	36.0	41.0	34.0	41.0
20	37.82058586081946	40.0	35.0	41.0	34.0	41.0
21	37.71745316180014	39.0	35.0	41.0	33.0	41.0
22	37.661747986390274	39.0	35.0	41.0	33.0	41.0
23	37.48857546368827	39.0	35.0	41.0	33.0	41.0
24	37.2073001045137	39.0	35.0	41.0	32.0	41.0
25	37.1306035301014	39.0	35.0	41.0	32.0	41.0
26	37.31169698120214	39.0	35.0	41.0	33.0	41.0
27	37.411210293660545	39.0	35.0	41.0	33.0	41.0
28	37.40514808193373	39.0	35.0	41.0	33.0	41.0
29	37.42074690787566	39.0	35.0	41.0	33.0	41.0
30	37.3010714218661	39.0	35.0	41.0	33.0	41.0
31	37.20660438957533	39.0	35.0	41.0	33.0	41.0
32	37.08348683565482	39.0	35.0	41.0	32.0	41.0
33	36.92573686329567	39.0	35.0	41.0	32.0	41.0
34	36.86402371480486	39.0	35.0	41.0	32.0	41.0
35	36.74436491760941	39.0	35.0	41.0	31.0	41.0
36	36.65253576099949	39.0	35.0	41.0	31.0	41.0
37	36.562155402064825	39.0	35.0	41.0	31.0	41.0
38	36.4383390040533	39.0	35.0	41.0	31.0	41.0
39	36.45955152984273	39.0	35.0	41.0	31.0	41.0
40	36.35263985747753	39.0	35.0	40.0	31.0	41.0
41	36.245888814948174	38.0	35.0	40.0	30.0	41.0
42	36.14111226773864	38.0	35.0	40.0	30.0	41.0
43	36.10241403696155	38.0	35.0	40.0	30.0	41.0
44	36.04829012668896	38.0	35.0	40.0	30.0	41.0
45	36.001669924462256	38.0	35.0	40.0	30.0	41.0
46	35.919412845797446	38.0	35.0	40.0	30.0	41.0
47	35.77639492409719	38.0	35.0	40.0	30.0	41.0
48	35.751552581238016	38.0	35.0	40.0	30.0	41.0
49	35.72055728122529	38.0	35.0	40.0	29.0	41.0
50	35.594644350940726	38.0	35.0	40.0	29.0	41.0
51	34.60555674926934	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	10.0
10	18.0
11	17.0
12	11.0
13	9.0
14	26.0
15	43.0
16	70.0
17	138.0
18	286.0
19	445.0
20	873.0
21	1301.0
22	2126.0
23	3188.0
24	4379.0
25	6158.0
26	8047.0
27	9896.0
28	11099.0
29	12867.0
30	15427.0
31	19673.0
32	25679.0
33	36938.0
34	71794.0
35	98173.0
36	74082.0
37	112330.0
38	195769.0
39	247730.0
40	115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.830671119798566	21.21784534893181	25.499777830162113	13.45170570110751
2	31.971074112937377	24.199301990349692	26.081800222378448	17.74782367433448
3	29.822493600882837	25.219614363227866	28.197003106205525	16.760888929683766
4	27.934049978826064	25.7012952605854	27.550520169474908	18.814134591113625
5	25.145036225157135	29.131993916927257	25.858483028519096	19.864486829396512
6	26.428197420326562	32.836597734910704	25.471302541080558	15.263902303682178
7	76.73141231175539	6.33580397318942	10.990105619332322	5.942678095722866
8	76.34329307852296	5.437632858606109	10.853048733423314	7.366025329447622
9	70.80083360626497	7.0833585403963175	13.732182083306387	8.383625770032314
10	43.49386581776232	22.96359961031619	19.227182740428443	14.315351831493045
11	33.04343472483275	22.831653673729512	24.556546917471728	19.568364683966013
12	29.961219368203217	20.317066607143232	30.129985000928315	19.591729023725236
13	23.88711686133473	25.487365524665023	30.836964888821207	19.78855272517904
14	17.299520405204408	30.503397216723027	29.487361352461495	22.70972102561107
15	15.785427744736245	25.016740966657835	39.362549883908436	19.835281404697483
16	17.580309702667915	24.724895329843978	35.61319918308255	22.081595784405554
17	18.5081034623031	24.016350865627928	29.671981358594635	27.80356431347434
18	20.512847257714924	24.653342039331363	31.518285725014238	23.315524977939475
19	22.886309539951977	26.61688532489992	27.665255766506803	22.8315493686413
20	25.629324749719938	26.44645081076345	27.50587759172068	20.418346847795927
21	22.346635013549232	27.33669473864274	28.77985993912755	21.53681030868048
22	19.776349029858377	25.654670886155166	26.02401520350966	28.544964880476797
23	20.035651479150456	27.596414408287668	27.587235560525116	24.780698552036764
24	22.07168680102553	23.384887861599665	29.949954418676448	24.593470918698358
25	19.48930142710222	25.94119696347027	28.620377459253216	25.949124150174296
26	18.50591305545067	27.443085928617773	28.46266816587847	25.588332850053092
27	21.254039214540967	27.404388740891555	27.035565948978125	24.306006095589357
28	17.443774342199962	25.591253392522994	30.110897169785737	26.854075095491307
29	18.48035830883902	22.729434687282914	30.746636682430644	28.04357032144742
30	20.726672688547094	23.923415032032093	28.622046340664593	26.727865938756224
31	22.76239509515753	25.21627660040512	23.49054891595722	28.530779388480127
32	22.48369189945824	24.703825702025394	26.64588213942253	26.16660025909384
33	21.630580582982	24.719888685609863	27.846433704728984	25.803097026679154
34	21.263009452127093	22.018282595861592	29.223260869111716	27.4954470828996
35	19.08824836293164	21.740831061220828	30.57390745635354	28.597013119493997
36	21.531282139005306	24.590654681316664	27.82661573796893	26.0514474417091
37	21.8524375056064	24.56071912100016	29.872142822871183	23.714700550522256
38	19.116202126572137	26.598110409021974	29.70963549543874	24.57605196896715
39	21.824900962318743	24.236643211929167	30.268919378425117	23.669536447326976
40	24.139430869716687	21.189161449673836	31.575653523530185	23.09575415707929
41	19.597987329017887	23.804194316207134	27.907765096596943	28.69005325817804
42	20.810951199821428	23.537486205652083	28.77224566768816	26.879316926838325
43	22.11716381948544	23.39417101445043	28.381414502162244	26.107250663901887
44	20.58471346349218	23.70979821137635	27.823486585322605	27.88200173980887
45	21.033851173327935	21.707870653346212	28.1629996474488	29.09527852587705
46	22.77094811239082	23.210281143934765	29.293562498565805	24.72520824510861
47	19.203192570139958	21.56132200441002	32.744704952196976	26.490780473253047
48	19.76570991086087	20.612771532220886	31.23009076628776	28.39142779063048
49	20.428255831175957	20.397590135241977	33.0516748268014	26.122479206780664
50	19.35099288013468	20.969599238990078	31.852687837818106	27.82672004305714
51	18.13500416177302	21.68325465252846	27.777488041421638	32.404253144276886
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	316.0
1	660.5
2	1005.0
3	3483.0
4	5961.0
5	4167.5
6	2374.0
7	2368.5
8	2363.0
9	2426.0
10	2489.0
11	2506.5
12	2524.0
13	2527.5
14	2531.0
15	2625.5
16	2720.0
17	2482.5
18	2245.0
19	2426.5
20	2608.0
21	2577.0
22	2546.0
23	2665.0
24	2784.0
25	3375.5
26	5436.5
27	6906.0
28	7376.0
29	7846.0
30	9244.5
31	10643.0
32	11855.0
33	13067.0
34	15145.0
35	17223.0
36	18728.5
37	20234.0
38	22409.0
39	24584.0
40	29192.0
41	33800.0
42	41976.5
43	50153.0
44	60583.0
45	71013.0
46	85265.0
47	99517.0
48	108858.0
49	118199.0
50	113654.5
51	109110.0
52	93234.0
53	77358.0
54	67455.0
55	57552.0
56	50862.5
57	44173.0
58	40850.5
59	37528.0
60	36946.5
61	36365.0
62	33358.0
63	30351.0
64	24633.5
65	18916.0
66	15322.5
67	11729.0
68	10172.5
69	8616.0
70	7476.5
71	6337.0
72	5427.5
73	4518.0
74	3799.5
75	2639.0
76	2197.0
77	1385.0
78	573.0
79	445.5
80	318.0
81	282.5
82	247.0
83	160.0
84	73.0
85	43.5
86	14.0
87	29.0
88	44.0
89	24.5
90	5.0
91	4.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	958726.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.083468407468033
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.16212993269893	16.41580502819886
2	12.139226976873251	5.847093787772213
3	5.600159711320778	4.046138084531077
4	3.1370620921915795	3.022053431582459
5	1.9654590691892622	2.366753569949556
6	1.299816164608153	1.8782448921514032
7	0.969160742873608	1.633852649292835
8	0.7288762725086524	1.404309494553215
9	0.5448829789355883	1.1810404808066082
>10	3.6002267351599735	18.442574200894647
>50	1.3365036426442731	23.713529946788178
>100	0.5073115918334075	16.41274892123982
>500	0.005248050950000767	0.9477238672980204
>1k	0.003061363054167114	1.5185911001003396
>5k	8.746751583334611E-4	1.1695405448407665
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	5947	0.6203023595897055	TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	5157	0.5379013399031631	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3449	0.3597482492390944	No Hit
CTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGCT	3426	0.35734923221024567	Illumina Paired End PCR Primer 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	2296	0.2394844825320269	TruSeq Adapter, Index 14 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	1584	0.16521925972592796	No Hit
CGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTG	1548	0.16146427655033868	No Hit
GCCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTG	1113	0.11609156317863498	No Hit
AGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTG	1002	0.10451369838723472	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC	982	0.10242759662301847	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3805049617930462	0.0
2	0.0	0.0	0.0	2.050846644401007	0.0
3	0.0	0.0	0.0	2.9286782667832103	0.0
4	0.0	0.0	0.0	3.88692911217595	0.0
5	1.043050882108131E-4	0.0	0.0	6.428322586432412	0.0
6	1.043050882108131E-4	0.0	0.0	7.750806799857311	0.0
7	1.043050882108131E-4	0.0	0.0	9.313609936519923	0.0
8	1.043050882108131E-4	0.0	0.0	11.822668833431033	0.0
9	1.043050882108131E-4	0.0	0.0	12.76005866118161	0.0
10	1.043050882108131E-4	0.0	0.0	14.6226346213621	0.0
11	1.043050882108131E-4	0.0	0.0	17.031352023414406	0.0
12	1.043050882108131E-4	0.0	0.0	19.03828622567866	0.0
13	1.043050882108131E-4	0.0	0.0	19.785632182709136	0.0
14	1.043050882108131E-4	0.0	0.0	20.065065514025907	0.0
15	1.043050882108131E-4	0.0	0.0	20.59712576898926	0.0
16	1.043050882108131E-4	0.0	0.0	21.7029683142003	0.0
17	1.043050882108131E-4	0.0	0.0	23.02148893427319	0.0
18	1.043050882108131E-4	0.0	0.0	24.720514516139126	0.0
19	1.043050882108131E-4	0.0	0.0	25.53044352609609	0.0
20	1.043050882108131E-4	0.0	0.0	26.275077550833085	0.0
21	1.043050882108131E-4	0.0	0.0	27.15968900394899	0.0
22	1.043050882108131E-4	0.0	0.0	28.07027242402939	0.0
23	1.043050882108131E-4	0.0	0.0	28.984715132373587	0.0
24	1.043050882108131E-4	0.0	0.0	29.732999835197962	0.0
25	1.043050882108131E-4	0.0	0.0	30.34318460123122	0.0
26	1.043050882108131E-4	0.0	0.0	30.900173772276958	0.0
27	1.043050882108131E-4	0.0	0.0	31.437344976562645	0.0
28	1.043050882108131E-4	0.0	0.0	31.96252109570409	0.0
29	1.043050882108131E-4	0.0	0.0	32.525142741513214	0.0
30	1.043050882108131E-4	0.0	0.0	33.16025642362886	0.0
31	3.129152646324393E-4	0.0	0.0	33.751457663607745	0.0
32	3.129152646324393E-4	0.0	0.0	34.301771309008	0.0
33	3.129152646324393E-4	0.0	0.0	34.83383156397135	0.0
34	3.129152646324393E-4	0.0	0.0	35.34513510638076	0.0
35	3.129152646324393E-4	0.0	0.0	35.94927017729779	0.0
36	5.215254410540655E-4	0.0	0.0	36.47382046590997	0.0
37	5.215254410540655E-4	0.0	0.0	37.03487753539593	0.0
38	6.258305292648786E-4	0.0	0.0	37.553378128891886	0.0
39	6.258305292648786E-4	0.0	0.0	38.13529621602001	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGA	30	2.165123E-6	45.000004	26
CTATGCA	20	7.0326997E-4	45.000004	11
TCGTTGA	20	7.0326997E-4	45.000004	25
ACACGAT	20	7.0326997E-4	45.000004	12
CCCTCCG	20	7.0326997E-4	45.000004	20
TCCGATA	20	7.0326997E-4	45.000004	11
CCGGCAT	40	6.8139343E-9	45.000004	15
CGTGACC	20	7.0326997E-4	45.000004	33
TAGAACG	30	2.165123E-6	45.000004	1
TCGGGTC	20	7.0326997E-4	45.000004	5
CCGTGAT	20	7.0326997E-4	45.000004	26
CCGTGAC	20	7.0326997E-4	45.000004	32
ATTAACG	20	7.0326997E-4	45.000004	1
TTATCGG	20	7.0326997E-4	45.000004	26
GCGTTAC	20	7.0326997E-4	45.000004	36
CCCACTA	20	7.0326997E-4	45.000004	14
CGTTATA	30	2.165123E-6	45.000004	14
CGTTAGC	30	2.165123E-6	45.000004	38
CGTTAGA	20	7.0326997E-4	45.000004	14
ACCTAGT	20	7.0326997E-4	45.000004	28
>>END_MODULE