FastQCFastQC Report
Sat 14 Jan 2017
SRR2934724.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934724.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880830
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC77540.880306074952034Illumina PCR Primer Index 8 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC57730.6554045616066664RNA PCR Primer, Index 40 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGCT44840.5090653133975909RNA PCR Primer, Index 40 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42640.4840888707242033No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGC26450.3002849585050464No Hit
TCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC24900.282687919348796Illumina PCR Primer Index 8 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTC22580.25634912525686RNA PCR Primer, Index 40 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCG22450.2548732445534326No Hit
ACTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC19070.21650034626431888TruSeq Adapter, Index 19 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTG17740.20140095137540728RNA PCR Primer, Index 40 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTG13010.14770159962762394Illumina PCR Primer Index 8 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTG11650.13226161688407526TruSeq Adapter, Index 19 (95% over 23bp)
GGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTG10450.1186381026985911RNA PCR Primer, Index 40 (95% over 24bp)
AGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTG10400.11807045627419592RNA PCR Primer, Index 40 (95% over 24bp)
GCAGCATATACACCTGTCTCTTATACACATCTGACGCACAGACTCTCGTAT9550.10842046705947798No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCC9500.10785282063508282No Hit
CGTTCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTC8950.10160870996673592TruSeq Adapter, Index 19 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAATG351.2115015E-745.0000041
GTCGACG253.89006E-545.0000041
GGTAACG351.2115015E-745.0000041
CAACGAT253.89006E-545.00000429
ATAATCG253.89006E-545.00000433
CGTTGTC253.89006E-545.00000424
GCACGTC253.89006E-545.00000413
ACCGACA253.89006E-545.00000410
TGAACGT253.89006E-545.00000424
CTAAGCG351.2115015E-745.0000041
AAGCGAA253.89006E-545.00000417
GCGTTGT253.89006E-545.00000423
CGTTCGG253.89006E-545.0000042
TCGATAA253.89006E-545.00000416
TGGCACG253.89006E-545.00000414
TCAATCG253.89006E-545.00000432
CCACTAA253.89006E-545.00000424
TCAGCGT253.89006E-545.0000042
CTACGTT253.89006E-545.00000419
CTACGAC253.89006E-545.00000421
Produced by FastQC (version 0.11.5)