##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934724.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	880830
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02177151096125	33.0	31.0	34.0	30.0	34.0
2	32.195725622424305	34.0	31.0	34.0	30.0	34.0
3	32.201028575321004	34.0	31.0	34.0	30.0	34.0
4	35.8092446896677	37.0	35.0	37.0	35.0	37.0
5	35.680953191875844	37.0	35.0	37.0	33.0	37.0
6	35.61550128855738	37.0	35.0	37.0	33.0	37.0
7	35.96278055924526	37.0	35.0	37.0	35.0	37.0
8	35.75583029642496	37.0	35.0	37.0	35.0	37.0
9	37.488670912661924	39.0	37.0	39.0	35.0	39.0
10	37.23727620539718	39.0	37.0	39.0	34.0	39.0
11	37.088891159474585	39.0	37.0	39.0	33.0	39.0
12	36.85045127890739	39.0	35.0	39.0	33.0	39.0
13	36.6736714235437	39.0	35.0	39.0	33.0	39.0
14	37.67300046547007	40.0	36.0	41.0	33.0	41.0
15	37.796438586333345	40.0	36.0	41.0	33.0	41.0
16	37.94325919871031	40.0	36.0	41.0	33.0	41.0
17	37.93273503400202	40.0	36.0	41.0	34.0	41.0
18	37.94042323717403	39.0	36.0	41.0	34.0	41.0
19	37.90012488221337	39.0	36.0	41.0	34.0	41.0
20	37.80868612558609	39.0	35.0	41.0	34.0	41.0
21	37.712639215285584	39.0	35.0	41.0	33.0	41.0
22	37.62812006857169	39.0	35.0	41.0	33.0	41.0
23	37.42991837244417	39.0	35.0	41.0	33.0	41.0
24	37.182161143466956	39.0	35.0	41.0	32.0	41.0
25	37.12357889717653	39.0	35.0	41.0	32.0	41.0
26	37.28745728460657	39.0	35.0	41.0	33.0	41.0
27	37.3619881248368	39.0	35.0	41.0	33.0	41.0
28	37.321553534734285	39.0	35.0	41.0	33.0	41.0
29	37.30669141605077	39.0	35.0	41.0	33.0	41.0
30	37.139930520077655	39.0	35.0	41.0	32.0	41.0
31	37.02265930996901	39.0	35.0	41.0	32.0	41.0
32	36.88997082297379	39.0	35.0	41.0	32.0	41.0
33	36.694462041483604	39.0	35.0	40.0	31.0	41.0
34	36.6275013339691	39.0	35.0	40.0	31.0	41.0
35	36.57035409783954	39.0	35.0	41.0	31.0	41.0
36	36.43747488164572	39.0	35.0	40.0	31.0	41.0
37	36.3842307823303	39.0	35.0	40.0	31.0	41.0
38	36.254497462620485	38.0	35.0	40.0	30.0	41.0
39	36.27601012681221	39.0	35.0	40.0	30.0	41.0
40	36.17910039394662	38.0	35.0	40.0	30.0	41.0
41	36.15781478832465	38.0	35.0	40.0	30.0	41.0
42	36.06660195497429	38.0	35.0	40.0	30.0	41.0
43	35.96633175527627	38.0	35.0	40.0	30.0	41.0
44	35.83607393027032	38.0	35.0	40.0	30.0	41.0
45	35.78570098656949	38.0	35.0	40.0	30.0	41.0
46	35.689192012079516	38.0	35.0	40.0	30.0	41.0
47	35.55264466469126	38.0	35.0	40.0	29.0	41.0
48	35.562988317836584	38.0	35.0	40.0	29.0	41.0
49	35.58260504297083	38.0	35.0	40.0	29.0	41.0
50	35.42759102210415	38.0	35.0	40.0	28.0	41.0
51	34.36194952487994	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	4.0
10	11.0
11	16.0
12	8.0
13	15.0
14	16.0
15	31.0
16	64.0
17	122.0
18	246.0
19	439.0
20	790.0
21	1291.0
22	2052.0
23	3088.0
24	4595.0
25	6212.0
26	7900.0
27	9441.0
28	10518.0
29	11907.0
30	14477.0
31	18418.0
32	24426.0
33	34707.0
34	65553.0
35	91968.0
36	71784.0
37	108727.0
38	185378.0
39	206499.0
40	124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.15632982527843	21.388463154070593	24.872449848438404	13.582757172212572
2	30.839208473825824	25.399225730277124	25.53239558144023	18.22917021445682
3	29.238672615601196	25.865263444705562	28.720638488698157	16.175425450995085
4	28.191478491876982	24.849857520747477	28.916703563684255	18.041960423691293
5	24.047091947367825	28.23620903011932	27.543112745932813	20.173586276580043
6	25.013566749543042	32.708354620074246	26.27919121737452	15.998887413008186
7	75.30635877524607	5.825868782852537	12.829944484179695	6.037827957721694
8	73.94934323308698	5.4867568089188605	12.203376360932303	8.360523597061862
9	68.74947492705743	5.999341530147702	15.488232689622288	9.762950853172576
10	41.60257938535245	21.90876786667121	21.605758205329064	14.882894542647277
11	30.172337454446374	22.78033218668756	26.31949411350658	20.72783624535949
12	25.733342415676123	20.544827038134486	33.08811007799462	20.63372046819477
13	21.920234324443992	26.591510280076747	33.93912559744786	17.5491297980314
14	17.39041585777051	30.11398340201855	31.368027882792365	21.12757285741857
15	14.806035216784169	24.13076303032367	41.328179103799826	19.735022649092336
16	16.212890115005166	24.185484145635368	36.258415358241656	23.34321038111781
17	16.192795431581576	24.196155898413995	30.228647979746377	29.382400690258052
18	18.540013396455617	23.50385431922164	35.005506170316636	22.95062611400611
19	19.786451415142537	25.399452788846883	30.183917441504036	24.630178354506548
20	21.655143444251447	25.733228886391245	30.07890285299093	22.532724816366382
21	19.81880726133306	27.942622299422137	31.70986455956314	20.528705879681663
22	18.281620744070935	25.69383422453822	28.36971946913706	27.654825562253784
23	17.519271596108215	27.540615101665473	29.536914046978417	25.403199255247895
24	20.05483464459657	22.694276988749248	32.58392652384683	24.666961842807353
25	16.533950932643073	26.727858951216465	30.24317972821089	26.495010387929568
26	16.43245575196122	29.41861653213446	29.556214025407858	24.592713690496463
27	19.30872018437156	28.1166626931417	28.520599888741298	24.054017233745444
28	15.817240557201732	27.548221563752367	31.402427256110716	25.23211062293519
29	18.162301465663067	26.205737770057784	29.6350033491139	25.99695741516524
30	19.173052688941112	28.492785213945936	26.643393163266467	25.690768933846485
31	20.768479729346183	30.242839140356253	22.60833532009582	26.380345810201746
32	22.853445046149652	29.330971924207848	23.5089631370412	24.306619892601297
33	20.506794727700008	27.822394786735238	23.633050645413984	28.037759840150766
34	17.861675919303384	27.662999670765075	26.72150131126324	27.753823098668303
35	18.558745728460657	25.611866080855556	28.11382446101972	27.715563729664066
36	20.29756025566795	27.371456467195713	25.371070467627128	26.959912809509213
37	19.318370173586278	26.191660138732786	28.365405356311662	26.124564331369278
38	18.99946641236107	28.886845361761065	26.22537833634186	25.888309889536004
39	21.57828411838834	25.427380992927123	26.73342188617554	26.260913002508996
40	20.5902387520861	23.405197370661764	29.7839537708752	26.22061010637694
41	16.7477265760703	24.36031924434908	26.311774122134807	32.580180057445816
42	20.18493920506795	22.234483384989158	28.901263580940707	28.67931382900219
43	22.343130910618395	22.785100416652476	27.70205374476346	27.16971492796567
44	19.529307584891523	25.833021127799917	26.86977055731526	27.7679007299933
45	20.531998228943156	24.08853013634867	25.91510280076746	29.464368833940714
46	23.185064087281315	24.457613841490414	27.783794829876367	24.573527241351904
47	17.27427539933926	23.193805842217	32.23062338930327	27.301295369140473
48	18.713486143750778	21.2725497542091	30.44571597243509	29.568248129605028
49	20.696615692017755	19.074622798950987	33.37965328156398	26.849108227467276
50	20.06834462949718	20.427097169714926	31.25336330506454	28.25119489572335
51	18.249491956450168	20.331505511846782	27.074577387237035	34.344425144466015
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	547.0
1	662.0
2	777.0
3	3844.0
4	6911.0
5	4627.0
6	2343.0
7	2423.0
8	2503.0
9	2682.5
10	2862.0
11	2859.5
12	2857.0
13	2860.0
14	2863.0
15	2754.5
16	2646.0
17	2634.5
18	2623.0
19	2553.0
20	2483.0
21	2455.0
22	2427.0
23	2900.5
24	3374.0
25	3203.0
26	4479.5
27	5927.0
28	6256.5
29	6586.0
30	8120.5
31	9655.0
32	9354.0
33	9053.0
34	11798.0
35	14543.0
36	15603.0
37	16663.0
38	19302.0
39	21941.0
40	27110.5
41	32280.0
42	40346.5
43	48413.0
44	59798.5
45	71184.0
46	87591.0
47	103998.0
48	116559.5
49	129121.0
50	124155.5
51	119190.0
52	96324.0
53	73458.0
54	62031.0
55	50604.0
56	42264.5
57	33925.0
58	29975.5
59	26026.0
60	22834.0
61	19642.0
62	17524.0
63	15406.0
64	13180.0
65	10954.0
66	9520.0
67	8086.0
68	7101.0
69	6116.0
70	4788.0
71	3460.0
72	2877.0
73	2294.0
74	2031.5
75	1418.5
76	1068.0
77	805.5
78	543.0
79	422.5
80	302.0
81	234.0
82	166.0
83	152.5
84	139.0
85	86.5
86	34.0
87	25.0
88	16.0
89	11.0
90	6.0
91	5.5
92	5.0
93	4.5
94	4.0
95	4.5
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	880830.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.80696405420316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.58396035265059	16.517459110546916
2	12.165878592163578	6.035970258472034
3	5.8874871722449535	4.381520479543883
4	3.5346999566437955	3.5074069906742444
5	2.1624374044967887	2.6821753481408113
6	1.5272771386446244	2.2732265447498077
7	1.1197940728088687	1.9445083918595552
8	0.826473367686986	1.6401836099173834
9	0.6348275347306458	1.4173329451213386
>10	3.9625288404469003	19.957324520665985
>50	1.2670213637181234	22.8284779988652
>100	0.3151380634233532	11.214041477398476
>500	0.006007030534462744	0.9986377323019066
>1k	0.0055449512625809945	3.0511638152447373
>5k	9.241585437634992E-4	1.5505707764976917
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	7754	0.880306074952034	Illumina PCR Primer Index 8 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	5773	0.6554045616066664	RNA PCR Primer, Index 40 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGCT	4484	0.5090653133975909	RNA PCR Primer, Index 40 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4264	0.4840888707242033	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGC	2645	0.3002849585050464	No Hit
TCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	2490	0.282687919348796	Illumina PCR Primer Index 8 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTC	2258	0.25634912525686	RNA PCR Primer, Index 40 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCG	2245	0.2548732445534326	No Hit
ACTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	1907	0.21650034626431888	TruSeq Adapter, Index 19 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTG	1774	0.20140095137540728	RNA PCR Primer, Index 40 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTG	1301	0.14770159962762394	Illumina PCR Primer Index 8 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTG	1165	0.13226161688407526	TruSeq Adapter, Index 19 (95% over 23bp)
GGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTG	1045	0.1186381026985911	RNA PCR Primer, Index 40 (95% over 24bp)
AGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTG	1040	0.11807045627419592	RNA PCR Primer, Index 40 (95% over 24bp)
GCAGCATATACACCTGTCTCTTATACACATCTGACGCACAGACTCTCGTAT	955	0.10842046705947798	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCC	950	0.10785282063508282	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTC	895	0.10160870996673592	TruSeq Adapter, Index 19 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.542215864582269	0.0
2	0.0	0.0	0.0	2.7210698999807	0.0
3	0.0	0.0	0.0	3.8139027962262864	0.0
4	0.0	0.0	0.0	4.955893872824495	0.0
5	0.0	0.0	0.0	8.285934856896336	0.0
6	0.0	0.0	0.0	9.946754765391733	0.0
7	0.0	0.0	0.0	11.833611480081286	0.0
8	0.0	0.0	0.0	14.853944575003123	0.0
9	0.0	0.0	0.0	15.95495157976	0.0
10	1.1352928487903455E-4	0.0	0.0	18.37028711556146	0.0
11	1.1352928487903455E-4	0.0	0.0	21.461235425678055	0.0
12	1.1352928487903455E-4	0.0	0.0	24.111463051894237	0.0
13	1.1352928487903455E-4	0.0	0.0	25.060794932052723	0.0
14	1.1352928487903455E-4	0.0	0.0	25.51638795227229	0.0
15	1.1352928487903455E-4	0.0	0.0	26.217544815685205	0.0
16	1.1352928487903455E-4	0.0	0.0	27.641542635922935	0.0
17	1.1352928487903455E-4	0.0	0.0	29.32620369424293	0.0
18	1.1352928487903455E-4	0.0	0.0	31.270279168511518	0.0
19	1.1352928487903455E-4	0.0	0.0	32.36436088689077	0.0
20	1.1352928487903455E-4	0.0	0.0	33.33253862833918	0.0
21	1.1352928487903455E-4	0.0	0.0	34.50472849471521	0.0
22	1.1352928487903455E-4	0.0	0.0	35.59733433239104	0.0
23	1.1352928487903455E-4	0.0	0.0	36.68233370797997	0.0
24	1.1352928487903455E-4	0.0	0.0	37.57467388712919	0.0
25	2.270585697580691E-4	0.0	0.0	38.30841365530239	0.0
26	2.270585697580691E-4	0.0	0.0	39.061339872620145	0.0
27	2.270585697580691E-4	0.0	0.0	39.701077392913504	0.0
28	2.270585697580691E-4	0.0	0.0	40.370218997990534	0.0
29	2.270585697580691E-4	0.0	0.0	41.06206646004337	0.0
30	2.270585697580691E-4	0.0	0.0	41.91682844589762	0.0
31	3.4058785463710365E-4	0.0	0.0	42.65794761758796	0.0
32	3.4058785463710365E-4	0.0	0.0	43.31607688203172	0.0
33	3.4058785463710365E-4	0.0	0.0	43.92686443468092	0.0
34	3.4058785463710365E-4	0.0	0.0	44.53197552308618	0.0
35	3.4058785463710365E-4	0.0	0.0	45.28263115470636	0.0
36	4.541171395161382E-4	0.0	0.0	45.92883984423782	0.0
37	4.541171395161382E-4	0.0	0.0	46.535880930486016	0.0
38	4.541171395161382E-4	0.0	0.0	47.12680085828139	0.0
39	4.541171395161382E-4	0.0	0.0	47.69058728699068	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCAATG	35	1.2115015E-7	45.000004	1
GTCGACG	25	3.89006E-5	45.000004	1
GGTAACG	35	1.2115015E-7	45.000004	1
CAACGAT	25	3.89006E-5	45.000004	29
ATAATCG	25	3.89006E-5	45.000004	33
CGTTGTC	25	3.89006E-5	45.000004	24
GCACGTC	25	3.89006E-5	45.000004	13
ACCGACA	25	3.89006E-5	45.000004	10
TGAACGT	25	3.89006E-5	45.000004	24
CTAAGCG	35	1.2115015E-7	45.000004	1
AAGCGAA	25	3.89006E-5	45.000004	17
GCGTTGT	25	3.89006E-5	45.000004	23
CGTTCGG	25	3.89006E-5	45.000004	2
TCGATAA	25	3.89006E-5	45.000004	16
TGGCACG	25	3.89006E-5	45.000004	14
TCAATCG	25	3.89006E-5	45.000004	32
CCACTAA	25	3.89006E-5	45.000004	24
TCAGCGT	25	3.89006E-5	45.000004	2
CTACGTT	25	3.89006E-5	45.000004	19
CTACGAC	25	3.89006E-5	45.000004	21
>>END_MODULE