FastQCFastQC Report
Sat 14 Jan 2017
SRR2934723.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934723.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1020000
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC59450.582843137254902TruSeq Adapter, Index 23 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC57340.562156862745098TruSeq Adapter, Index 23 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48190.4724509803921568No Hit
CTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGCT38130.3738235294117647TruSeq Adapter, Index 20 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC23740.23274509803921567TruSeq Adapter, Index 23 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTG17760.17411764705882354TruSeq Adapter, Index 20 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC14520.1423529411764706TruSeq Adapter, Index 20 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTG10860.10647058823529411TruSeq Adapter, Index 23 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTG10360.1015686274509804TruSeq Adapter, Index 20 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGG950.045.0000042
ACTATCG253.890496E-545.01
TCGTTAG351.2117562E-745.01
CGAACTG253.890496E-545.029
CGTGCAA207.032914E-445.036
TAGCGTA253.890496E-545.01
CGGTTAT207.032914E-445.06
TATATCG253.890496E-545.013
TAACGGT207.032914E-445.037
ACCCTTA351.2117562E-745.022
TCTAGTC207.032914E-445.015
CGTTAGG1000.045.02
AAACGCC253.890496E-545.025
TACGCTA207.032914E-445.026
ACGATAT207.032914E-445.032
CCGTAGG700.045.02
TACGATC253.890496E-545.011
AACGCGC207.032914E-445.01
CGTACAG207.032914E-445.01
AATTACG207.032914E-445.01
Produced by FastQC (version 0.11.5)