##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934723.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1020000
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09208823529412	33.0	31.0	34.0	30.0	34.0
2	32.26712156862745	34.0	31.0	34.0	30.0	34.0
3	32.26794901960784	34.0	31.0	34.0	30.0	34.0
4	35.851125490196075	37.0	35.0	37.0	35.0	37.0
5	35.73594509803922	37.0	35.0	37.0	35.0	37.0
6	35.66088529411765	37.0	35.0	37.0	33.0	37.0
7	36.009549019607846	37.0	35.0	37.0	35.0	37.0
8	35.83922352941177	37.0	35.0	37.0	35.0	37.0
9	37.47835980392157	39.0	37.0	39.0	35.0	39.0
10	37.2837568627451	39.0	37.0	39.0	34.0	39.0
11	37.16316764705882	39.0	37.0	39.0	34.0	39.0
12	36.9418137254902	39.0	35.0	39.0	33.0	39.0
13	36.79354019607843	39.0	35.0	39.0	33.0	39.0
14	37.85806176470588	40.0	36.0	41.0	33.0	41.0
15	37.953290196078434	40.0	36.0	41.0	33.0	41.0
16	38.05123235294118	40.0	36.0	41.0	33.0	41.0
17	38.039261764705884	40.0	36.0	41.0	34.0	41.0
18	38.01057254901961	40.0	36.0	41.0	34.0	41.0
19	37.969979411764704	40.0	36.0	41.0	34.0	41.0
20	37.871291176470585	40.0	36.0	41.0	34.0	41.0
21	37.79051862745098	39.0	35.0	41.0	33.0	41.0
22	37.72839117647059	39.0	35.0	41.0	33.0	41.0
23	37.517316666666666	39.0	35.0	41.0	33.0	41.0
24	37.26946176470588	39.0	35.0	41.0	32.0	41.0
25	37.19599901960785	39.0	35.0	41.0	32.0	41.0
26	37.34977647058823	39.0	35.0	41.0	33.0	41.0
27	37.40330098039216	39.0	35.0	41.0	33.0	41.0
28	37.37842647058824	39.0	35.0	41.0	33.0	41.0
29	37.396593137254904	39.0	35.0	41.0	33.0	41.0
30	37.24762843137255	39.0	35.0	41.0	33.0	41.0
31	37.151466666666664	39.0	35.0	41.0	33.0	41.0
32	37.05774411764706	39.0	35.0	41.0	32.0	41.0
33	36.865249019607845	39.0	35.0	41.0	32.0	41.0
34	36.81413725490196	39.0	35.0	41.0	31.0	41.0
35	36.68470980392157	39.0	35.0	41.0	31.0	41.0
36	36.58902254901961	39.0	35.0	41.0	31.0	41.0
37	36.48587647058824	39.0	35.0	41.0	31.0	41.0
38	36.41194215686274	39.0	35.0	40.0	31.0	41.0
39	36.451099019607845	39.0	35.0	41.0	31.0	41.0
40	36.35982941176471	39.0	35.0	40.0	31.0	41.0
41	36.268260784313725	38.0	35.0	40.0	31.0	41.0
42	36.116669607843136	38.0	35.0	40.0	30.0	41.0
43	36.00147156862745	38.0	35.0	40.0	30.0	41.0
44	35.91424509803922	38.0	35.0	40.0	30.0	41.0
45	35.88160784313725	38.0	35.0	40.0	30.0	41.0
46	35.8210637254902	38.0	35.0	40.0	30.0	41.0
47	35.6784862745098	38.0	35.0	40.0	29.0	41.0
48	35.63280784313726	38.0	35.0	40.0	29.0	41.0
49	35.59928921568628	38.0	35.0	40.0	29.0	41.0
50	35.465555882352945	37.0	35.0	40.0	29.0	41.0
51	34.43622450980392	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	8.0
9	15.0
10	18.0
11	9.0
12	11.0
13	17.0
14	19.0
15	34.0
16	62.0
17	155.0
18	275.0
19	501.0
20	925.0
21	1497.0
22	2202.0
23	3326.0
24	4574.0
25	6425.0
26	8783.0
27	10796.0
28	12007.0
29	13946.0
30	16440.0
31	21028.0
32	27377.0
33	39040.0
34	75850.0
35	100995.0
36	81084.0
37	122390.0
38	210932.0
39	259112.0
40	145.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.98980392156863	21.278039215686277	25.95823529411765	13.77392156862745
2	32.73470588235294	24.41313725490196	25.460882352941177	17.39127450980392
3	29.748137254901962	24.828235294117647	29.189019607843136	16.234607843137255
4	27.90794117647059	26.004215686274513	27.479313725490194	18.608529411764707
5	25.139607843137256	28.80872549019608	26.61980392156863	19.431862745098037
6	25.619019607843136	33.459215686274504	25.255	15.666764705882352
7	77.66960784313726	5.965098039215686	10.65813725490196	5.707156862745098
8	76.26009803921569	4.58343137254902	11.498823529411764	7.657647058823529
9	69.8778431372549	6.850588235294118	14.866960784313726	8.404607843137255
10	41.4964705882353	22.431960784313727	20.439607843137257	15.631960784313726
11	31.277352941176474	21.756176470588233	26.273529411764706	20.69294117647059
12	28.882941176470588	18.70872549019608	32.12205882352941	20.286274509803924
13	23.767254901960786	23.810000000000002	32.71568627450981	19.70705882352941
14	18.74578431372549	27.403823529411763	31.343039215686275	22.50735294117647
15	17.2171568627451	23.122352941176473	38.9814705882353	20.679019607843138
16	18.937745098039215	22.824019607843137	35.22264705882353	23.015588235294118
17	18.839803921568627	22.65843137254902	30.59647058823529	27.90529411764706
18	20.52401960784314	23.82029411764706	32.66147058823529	22.99421568627451
19	22.375882352941176	25.57029411764706	29.34441176470588	22.709411764705884
20	23.77098039215686	26.260392156862743	28.08362745098039	21.884999999999998
21	21.99921568627451	27.10549019607843	29.661176470588234	21.234117647058824
22	19.87872549019608	25.28666666666667	27.54313725490196	27.291470588235295
23	19.966764705882355	25.421764705882353	29.062549019607843	25.548921568627453
24	22.707549019607843	21.670882352941177	30.526470588235295	25.095098039215685
25	19.754019607843137	24.804411764705883	28.679411764705883	26.762156862745094
26	20.300196078431373	25.994607843137256	29.878235294117644	23.826960784313727
27	21.43764705882353	25.30980392156863	29.945	23.307549019607844
28	18.429901960784314	24.440686274509805	31.22490196078431	25.904509803921567
29	21.79480392156863	22.342745098039217	30.44607843137255	25.416372549019606
30	22.737450980392158	22.31362745098039	29.442745098039214	25.506176470588237
31	22.696470588235293	23.825196078431375	26.94656862745098	26.531764705882356
32	25.084313725490198	22.606862745098038	29.12970588235294	23.179117647058824
33	22.9521568627451	22.40872549019608	28.453039215686278	26.18607843137255
34	21.340098039215686	20.486078431372547	31.858235294117648	26.31558823529412
35	21.185588235294116	20.477450980392156	33.01941176470588	25.317549019607842
36	24.542058823529413	21.314607843137253	29.997843137254904	24.14549019607843
37	21.665686274509806	22.4578431372549	33.408725490196076	22.467745098039217
38	20.202745098039216	23.14941176470588	30.371764705882352	26.27607843137255
39	23.952156862745095	20.83235294117647	30.99735294117647	24.21813725490196
40	23.694019607843135	19.200098039215685	33.00294117647059	24.102941176470587
41	21.451764705882354	21.020098039215686	30.340392156862745	27.18774509803922
42	24.36941176470588	20.236470588235296	30.536470588235293	24.857647058823527
43	24.24264705882353	19.498431372549017	31.044901960784316	25.214019607843134
44	21.232352941176472	21.59264705882353	29.249901960784314	27.925098039215683
45	21.340392156862745	20.091666666666665	30.347156862745102	28.22078431372549
46	24.031960784313725	20.752745098039217	30.432156862745096	24.78313725490196
47	20.02901960784314	20.497647058823528	34.21127450980392	25.262058823529408
48	21.41480392156863	18.419607843137257	32.88039215686275	27.285196078431373
49	22.732156862745097	17.24480392156863	34.28892156862745	25.734117647058824
50	21.022058823529413	17.911078431372548	33.245098039215684	27.82176470588235
51	19.395588235294117	18.51411764705882	29.555392156862748	32.53490196078431
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	435.0
1	633.0
2	831.0
3	4597.5
4	8364.0
5	5550.5
6	2737.0
7	2706.0
8	2675.0
9	2806.0
10	2937.0
11	3039.5
12	3142.0
13	3090.0
14	3038.0
15	2968.5
16	2899.0
17	2935.0
18	2971.0
19	2736.0
20	2501.0
21	2505.5
22	2510.0
23	3016.5
24	3523.0
25	4272.5
26	5446.0
27	5870.0
28	6610.0
29	7350.0
30	8600.0
31	9850.0
32	11851.5
33	13853.0
34	14858.0
35	15863.0
36	17026.5
37	18190.0
38	20929.0
39	23668.0
40	28794.5
41	33921.0
42	41568.0
43	49215.0
44	59247.0
45	69279.0
46	86109.0
47	102939.0
48	116377.0
49	129815.0
50	127546.5
51	125278.0
52	103890.0
53	82502.0
54	69161.5
55	55821.0
56	51964.0
57	48107.0
58	44583.0
59	41059.0
60	39257.5
61	37456.0
62	35480.0
63	33504.0
64	28748.0
65	23992.0
66	19842.0
67	15692.0
68	14071.0
69	12450.0
70	10462.0
71	8474.0
72	6849.0
73	5224.0
74	4300.5
75	2594.5
76	1812.0
77	1388.5
78	965.0
79	691.0
80	417.0
81	359.0
82	301.0
83	197.5
84	94.0
85	68.5
86	43.0
87	31.5
88	20.0
89	15.0
90	10.0
91	6.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1020000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.58213727198368
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.63057614378086	17.301346826935468
2	12.202476435996486	6.243308544876165
3	5.667571970293326	4.3496581242867265
4	3.211016700454658	3.2857868005465267
5	1.9882071355878002	2.5431293933872285
6	1.3641655754838633	2.0938962608245686
7	0.9797501290779033	1.7544871606020256
8	0.7144782351002137	1.4622304230542635
9	0.5634499711347458	1.297282905671788
>10	4.065728177144571	22.80579081009888
>50	1.374194846236918	24.757779394024084
>100	0.22948395303123534	8.414688649643294
>500	0.005030845513332231	0.814768610498765
>1k	0.0030959049312813728	1.7196270673588758
>5k	7.739762328203432E-4	1.1562190281913824
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	5945	0.582843137254902	TruSeq Adapter, Index 23 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	5734	0.562156862745098	TruSeq Adapter, Index 23 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4819	0.4724509803921568	No Hit
CTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGCT	3813	0.3738235294117647	TruSeq Adapter, Index 20 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	2374	0.23274509803921567	TruSeq Adapter, Index 23 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTG	1776	0.17411764705882354	TruSeq Adapter, Index 20 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	1452	0.1423529411764706	TruSeq Adapter, Index 20 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTG	1086	0.10647058823529411	TruSeq Adapter, Index 23 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTG	1036	0.1015686274509804	TruSeq Adapter, Index 20 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3996078431372549	0.0
2	0.0	0.0	0.0	2.0245098039215685	0.0
3	0.0	0.0	0.0	2.8823529411764706	0.0
4	0.0	0.0	0.0	3.8194117647058823	0.0
5	0.0	0.0	0.0	6.2880392156862746	0.0
6	0.0	0.0	0.0	7.708333333333333	0.0
7	0.0	0.0	0.0	9.351274509803922	0.0
8	0.0	0.0	0.0	12.12343137254902	0.0
9	0.0	0.0	0.0	13.236862745098039	0.0
10	0.0	0.0	0.0	15.386372549019608	0.0
11	0.0	0.0	0.0	18.09029411764706	0.0
12	0.0	0.0	0.0	20.19970588235294	0.0
13	0.0	0.0	0.0	21.00549019607843	0.0
14	0.0	0.0	0.0	21.321666666666665	0.0
15	0.0	0.0	0.0	21.899607843137254	0.0
16	0.0	0.0	0.0	23.190196078431374	0.0
17	0.0	0.0	0.0	24.705	0.0
18	0.0	0.0	0.0	26.453039215686275	0.0
19	0.0	0.0	0.0	27.369313725490198	0.0
20	0.0	0.0	0.0	28.19843137254902	0.0
21	9.80392156862745E-5	0.0	0.0	29.225490196078432	0.0
22	9.80392156862745E-5	0.0	0.0	30.293039215686274	0.0
23	9.80392156862745E-5	0.0	0.0	31.271372549019606	0.0
24	9.80392156862745E-5	0.0	0.0	32.093333333333334	0.0
25	9.80392156862745E-5	0.0	0.0	32.78078431372549	0.0
26	9.80392156862745E-5	0.0	0.0	33.40078431372549	0.0
27	9.80392156862745E-5	0.0	0.0	34.007745098039216	0.0
28	9.80392156862745E-5	0.0	0.0	34.64029411764706	0.0
29	9.80392156862745E-5	0.0	0.0	35.26882352941176	0.0
30	9.80392156862745E-5	0.0	0.0	35.996274509803925	0.0
31	1.96078431372549E-4	0.0	0.0	36.663039215686275	0.0
32	1.96078431372549E-4	0.0	0.0	37.26833333333333	0.0
33	1.96078431372549E-4	0.0	0.0	37.86147058823529	0.0
34	1.96078431372549E-4	0.0	0.0	38.453039215686275	0.0
35	1.96078431372549E-4	0.0	0.0	39.058235294117644	0.0
36	1.96078431372549E-4	0.0	0.0	39.676764705882356	0.0
37	2.941176470588235E-4	0.0	0.0	40.27392156862745	0.0
38	2.941176470588235E-4	0.0	0.0	40.86558823529412	0.0
39	3.92156862745098E-4	0.0	0.0	41.45852941176471	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAAGG	95	0.0	45.000004	2
ACTATCG	25	3.890496E-5	45.0	1
TCGTTAG	35	1.2117562E-7	45.0	1
CGAACTG	25	3.890496E-5	45.0	29
CGTGCAA	20	7.032914E-4	45.0	36
TAGCGTA	25	3.890496E-5	45.0	1
CGGTTAT	20	7.032914E-4	45.0	6
TATATCG	25	3.890496E-5	45.0	13
TAACGGT	20	7.032914E-4	45.0	37
ACCCTTA	35	1.2117562E-7	45.0	22
TCTAGTC	20	7.032914E-4	45.0	15
CGTTAGG	100	0.0	45.0	2
AAACGCC	25	3.890496E-5	45.0	25
TACGCTA	20	7.032914E-4	45.0	26
ACGATAT	20	7.032914E-4	45.0	32
CCGTAGG	70	0.0	45.0	2
TACGATC	25	3.890496E-5	45.0	11
AACGCGC	20	7.032914E-4	45.0	1
CGTACAG	20	7.032914E-4	45.0	1
AATTACG	20	7.032914E-4	45.0	1
>>END_MODULE