##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934717.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	443006
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.970846895978834	33.0	31.0	34.0	30.0	34.0
2	32.15741547518544	33.0	31.0	34.0	30.0	34.0
3	32.064231635688905	33.0	31.0	34.0	30.0	34.0
4	35.81422373511871	37.0	35.0	37.0	35.0	37.0
5	32.131178810219275	37.0	35.0	37.0	10.0	37.0
6	33.96855121601062	37.0	35.0	37.0	19.0	37.0
7	20.703579635490264	32.0	0.0	37.0	0.0	37.0
8	28.053188895861457	35.0	17.0	37.0	17.0	37.0
9	34.79518787555925	37.0	32.0	39.0	32.0	39.0
10	36.188566294813164	37.0	35.0	39.0	32.0	39.0
11	36.59146828711123	38.0	35.0	39.0	32.0	39.0
12	36.92191527879984	39.0	37.0	39.0	33.0	39.0
13	36.66240637824318	39.0	35.0	39.0	33.0	39.0
14	37.954975779109084	40.0	37.0	41.0	33.0	41.0
15	38.15793465551257	40.0	37.0	41.0	33.0	41.0
16	38.25509135316452	40.0	37.0	41.0	33.0	41.0
17	38.23280045868453	40.0	37.0	41.0	34.0	41.0
18	38.146905459519736	40.0	37.0	41.0	33.0	41.0
19	38.0995404125452	40.0	37.0	41.0	34.0	41.0
20	38.079339783208354	40.0	37.0	41.0	34.0	41.0
21	37.976756522485026	40.0	37.0	41.0	33.0	41.0
22	37.99148769994086	40.0	36.0	41.0	33.0	41.0
23	37.94919707633757	40.0	36.0	41.0	33.0	41.0
24	37.80398685345119	40.0	36.0	41.0	33.0	41.0
25	37.67170873532187	40.0	36.0	41.0	33.0	41.0
26	37.52292294009562	39.0	36.0	41.0	33.0	41.0
27	37.48474964221703	39.0	35.0	41.0	33.0	41.0
28	37.39162900728207	39.0	35.0	41.0	33.0	41.0
29	37.3495708861731	39.0	35.0	41.0	33.0	41.0
30	37.191706207139404	39.0	35.0	41.0	32.0	41.0
31	37.063044292853824	39.0	35.0	41.0	31.0	41.0
32	36.97088075556539	39.0	35.0	41.0	31.0	41.0
33	36.84009923116165	39.0	35.0	41.0	31.0	41.0
34	36.65277671182783	39.0	35.0	41.0	30.0	41.0
35	36.545019706279376	39.0	35.0	41.0	30.0	41.0
36	36.416867943097834	39.0	35.0	41.0	30.0	41.0
37	36.3294650636786	39.0	35.0	41.0	30.0	41.0
38	36.203733132282636	39.0	35.0	40.0	30.0	41.0
39	36.0977571409868	39.0	35.0	40.0	29.0	41.0
40	35.983968614420576	39.0	35.0	40.0	29.0	41.0
41	35.8689137393173	39.0	35.0	40.0	28.0	41.0
42	35.78853108084315	38.0	35.0	40.0	28.0	41.0
43	35.704026582032746	38.0	35.0	40.0	28.0	41.0
44	35.55231757583419	38.0	34.0	40.0	27.0	41.0
45	35.44241838710988	38.0	34.0	40.0	27.0	41.0
46	35.31738847780843	38.0	34.0	40.0	26.0	41.0
47	35.246594854245764	38.0	34.0	40.0	26.0	41.0
48	35.135801772436494	38.0	34.0	40.0	26.0	41.0
49	35.019715308596275	38.0	34.0	40.0	26.0	41.0
50	34.838510087899486	37.0	34.0	40.0	25.0	41.0
51	32.96567992307102	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	10.0
9	27.0
10	18.0
11	10.0
12	10.0
13	8.0
14	20.0
15	33.0
16	41.0
17	75.0
18	176.0
19	314.0
20	548.0
21	868.0
22	1350.0
23	2098.0
24	2899.0
25	3972.0
26	4940.0
27	5713.0
28	6632.0
29	7625.0
30	9516.0
31	12462.0
32	16369.0
33	24419.0
34	32838.0
35	40253.0
36	51039.0
37	82889.0
38	83246.0
39	52579.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.96780179049494	17.563644736188674	22.92993774350686	15.53861572980953
2	31.238628822182996	24.254299038839203	26.312961901193212	18.19411023778459
3	32.914226895346786	23.412323986582575	27.37886168584624	16.294587432224393
4	29.224209152923436	25.947278366432958	26.158787917093672	18.66972456354993
5	23.016166823925634	37.579400730464144	22.7428070951635	16.66162535044672
6	27.413624194706166	35.13564150372681	24.455876444111365	12.994857857455655
7	49.64695737755245	43.079326239373735	4.8450811049963205	2.428635278077498
8	85.34218498169325	4.829505695182458	7.102612605698343	2.7256967174259494
9	79.30976104161117	7.362428499839731	8.9310302794996	4.396780179049493
10	44.6677923098107	25.846602529085384	16.43612050401123	13.04948465709268
11	34.43181356460183	25.301688916177206	23.121583003390473	17.144914515830486
12	31.852164530502975	22.22159519284163	26.93868706067186	18.987553215983528
13	26.64546304113263	24.179356487271054	28.58132846959183	20.593852002004486
14	21.54079177257193	25.699877654027258	29.879505017990727	22.879825555410086
15	21.00648749678334	25.7150016026871	32.58375733060049	20.694753569929077
16	25.045258980691003	24.586574448201603	30.513130747664817	19.85503582344257
17	24.77280217423692	24.838941233301583	27.90503966086238	22.48321693159912
18	25.283630470016206	24.93374807564683	28.88087294528742	20.90174850904954
19	26.166688487289115	26.564200033408124	25.881365037945308	21.387746441357454
20	27.617233175171442	25.600781930718767	27.4775059480007	19.304478946109082
21	26.3416296844738	26.809343440043705	27.496241585892744	19.352785289589757
22	25.87504458178896	24.41885662948132	27.85425028103457	21.851848507695156
23	24.85338799023038	25.507555202412608	28.82918064315156	20.809876164205452
24	23.544827835288913	24.90485456178923	29.93210024243464	21.618217360487215
25	23.76581807018415	26.28000523694939	27.72784115790757	22.226335534958892
26	23.06086147817411	26.72356582077895	27.436422982984425	22.77914971806251
27	23.370563829835262	27.063064608605753	27.978627828968456	21.58774373259053
28	21.79383574940294	27.627165320559993	28.37049611066216	22.208502819374907
29	23.2087150061173	27.139135813058967	27.990365818973107	21.661783361850627
30	23.930375660826265	25.816580362342723	28.25311621061566	21.999927766215354
31	25.501009015679244	25.688591125176636	27.399177437777368	21.411222421366755
32	26.6720992492201	26.538015286474675	26.443208444129425	20.3466770201758
33	25.610036884376285	26.21725213653991	26.458783853943284	21.71392712514052
34	22.63942249089177	26.0635296135944	29.05987729285834	22.237170602655496
35	23.503970600849648	26.613183568619835	28.30480851275152	21.578037317778993
36	25.021105808950665	28.34972889757701	25.846376798508373	20.78278849496395
37	23.76649526191519	28.860105732202275	27.20527487212363	20.168124133758912
38	23.732861405940326	27.988334243779995	26.84071999024844	21.43808436003124
39	23.858819067913302	26.985413290113453	26.790382071574655	22.365385570398594
40	24.564001390500355	25.74795826693092	28.13144742960592	21.556592912962806
41	21.22409177302339	25.83734757542787	28.911120842607097	24.02743980894164
42	22.427912940231057	26.13824643458554	27.48676090165822	23.94707972352519
43	22.67102477167352	25.800553491374835	27.89939639643707	23.629025340514577
44	22.741001250547395	26.995796896656028	26.97525541414789	23.28794643864869
45	23.120228619928398	26.444788558168515	26.7985083723471	23.63647444955599
46	22.373511871171043	28.07840074400798	26.29377480214715	23.254312582673826
47	22.188412798020792	26.67006767402699	28.59013196209532	22.551387565856896
48	22.421366753497697	26.4933206322262	28.70457736464066	22.380735249635446
49	22.73332641092897	26.744333033864105	27.894881784896818	22.627458770310106
50	21.17443104608064	27.081574515920774	28.770264962551295	22.973729475447286
51	21.68345349724383	26.21725213653991	27.473217067037464	24.626077299178792
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	205.0
1	730.0
2	1255.0
3	2665.5
4	4076.0
5	2818.0
6	1560.0
7	1379.0
8	1198.0
9	1183.5
10	1169.0
11	1203.5
12	1238.0
13	1203.0
14	1168.0
15	1169.0
16	1170.0
17	1173.0
18	1176.0
19	1254.5
20	1333.0
21	1435.0
22	1537.0
23	1854.5
24	2172.0
25	2564.0
26	3654.5
27	4353.0
28	5371.5
29	6390.0
30	7479.5
31	8569.0
32	9735.5
33	10902.0
34	12286.0
35	13670.0
36	14460.5
37	15251.0
38	16559.5
39	17868.0
40	19841.0
41	21814.0
42	23368.5
43	24923.0
44	26385.5
45	27848.0
46	28977.0
47	30106.0
48	30186.0
49	30266.0
50	29891.0
51	29516.0
52	28540.0
53	27564.0
54	27048.0
55	26532.0
56	26145.0
57	25758.0
58	25425.0
59	25092.0
60	23651.5
61	22211.0
62	19578.5
63	16946.0
64	14287.0
65	11628.0
66	9837.5
67	8047.0
68	6820.5
69	5594.0
70	4657.5
71	3721.0
72	3136.5
73	2552.0
74	2126.5
75	1311.0
76	921.0
77	705.0
78	489.0
79	383.5
80	278.0
81	199.0
82	120.0
83	99.0
84	78.0
85	60.0
86	42.0
87	32.0
88	22.0
89	17.0
90	12.0
91	8.0
92	4.0
93	2.5
94	1.0
95	1.5
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	443006.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.46096699772022
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.10581304509233	28.651938340131966
2	8.850171947495	7.3387337407847895
3	4.767943665197693	5.930506648492523
4	3.3975698735232776	5.634661295943882
5	2.6912195793049447	5.579028308059039
6	2.1427341821174934	5.330389872579625
7	1.7744258165349132	5.149858715347976
8	1.546538241852393	5.129677680492344
9	1.2716196479981117	4.745032223337207
>10	4.416109808404935	24.703783269787337
>50	0.026330422601718934	0.7609275207563385
>100	0.008403326362250333	0.5702310938512655
>500	5.602217574833555E-4	0.175598658636072
>1k	5.602217574833555E-4	0.29963263179964666
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1290	0.29119244434612623	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	756	0.17065231622145072	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0234759800093001	0.0
2	0.0	0.0	0.0	0.10315887369471294	0.0
3	0.0	0.0	0.0	0.1388243048626881	0.0
4	0.0	0.0	0.0	0.18058446161000077	0.0
5	0.0	0.0	0.0	0.3367900209026514	0.0
6	0.0	0.0	0.0	0.49186692731023957	0.0
7	0.0	0.0	0.0	0.6295625792878652	0.0
8	0.0	0.0	0.0	0.9613865274962415	0.0
9	0.0	0.0	0.0	1.1162377033268172	0.0
10	0.0	0.0	0.0	1.484630005011219	0.0
11	0.0	0.0	0.0	1.887559084978533	0.0
12	0.0	0.0	0.0	2.234506981846747	0.0
13	0.0	0.0	0.0	2.37649151478761	0.0
14	0.0	0.0	0.0	2.448048107700573	0.0
15	0.0	0.0	0.0	2.5681367746712236	0.0
16	0.0	0.0	0.0	2.7918357764906117	0.0
17	0.0	0.0	0.0	3.0575206656343257	0.0
18	0.0	0.0	0.0	3.349390301711489	0.0
19	0.0	0.0	0.0	3.51643092870074	0.0
20	0.0	0.0	0.0	3.698595504349828	0.0
21	0.0	0.0	0.0	3.9617973571464042	0.0
22	0.0	0.0	0.0	4.229513821483231	0.0
23	0.0	0.0	0.0	4.502873550245369	0.0
24	0.0	0.0	0.0	4.720026365331395	0.0
25	0.0	0.0	0.0	4.919797925987458	0.0
26	0.0	0.0	0.0	5.111217455294059	0.0
27	0.0	0.0	0.0	5.332884881920335	0.0
28	0.0	0.0	0.0	5.537622515270674	0.0
29	0.0	0.0	0.0	5.7547753303567	0.0
30	0.0	0.0	0.0	6.094725579337526	0.0
31	0.0	0.0	0.0	6.343254944628289	0.0
32	0.0	0.0	0.0	6.586592506647765	0.0
33	0.0	0.0	0.0	6.821352306740766	0.0
34	0.0	0.0	0.0	7.07349336126373	0.0
35	0.0	0.0	0.0	7.3771009873455435	0.0
36	0.0	0.0	0.0	7.623824508020207	0.0
37	0.0	0.0	0.0	7.8901865888949585	0.0
38	0.0	0.0	0.0	8.172349810160584	0.0
39	0.0	0.0	0.0	8.586565418978523	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGG	30	2.1624692E-6	45.000004	2
TCGATCA	30	2.1624692E-6	45.000004	17
CGATGTC	30	2.1624692E-6	45.000004	10
ATGATCG	25	3.8869115E-5	45.0	27
CGACGTT	20	7.028588E-4	45.0	27
ACGCACT	20	7.028588E-4	45.0	40
TTAACGG	25	3.8869115E-5	45.0	2
AGGCGAT	270	0.0	42.500004	7
GTACGGG	90	0.0	42.5	3
CGTTTTA	315	0.0	40.714287	1
TAACGGG	75	0.0	39.000004	3
GACCGAC	35	6.2395993E-6	38.571426	23
GCCTCGA	35	6.2395993E-6	38.571426	14
AACACGT	70	0.0	38.571426	41
TAGGACC	100	0.0	38.25	6
GATCGTG	30	1.1387895E-4	37.500004	29
AAGGTAT	120	0.0	37.500004	6
TATTGCG	30	1.1387895E-4	37.500004	1
TTACGCA	30	1.1387895E-4	37.500004	1
TCGCGCA	30	1.1387895E-4	37.500004	34
>>END_MODULE