FastQCFastQC Report
Sat 14 Jan 2017
SRR2934716.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934716.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437216
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32220.7369355192856619No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18600.4254190148576448No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6680.15278489350801433No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGAT207.028485E-445.00000435
CGGGTAT253.8868267E-545.06
ACGTTGA253.8868267E-545.01
CGTTAAG253.8868267E-545.02
CGGTAAG302.1624055E-644.9999962
CGTTATT2650.044.1509441
CGTTTTA5600.042.991071
TTAATCG950.042.6315820
AGGCGAT2350.041.170217
CGTTTTT11050.040.7239841
TTACACG1350.040.00000434
CTATACG403.451678E-739.3750041
CGAGATA1600.039.37500419
CTAAGGC750.039.04
TAACGGG750.039.03
CTTACAC1500.039.033
TCGTTGA1050.038.5714324
ACACGCG1400.038.57142636
GTCTTAC1550.037.74193631
TTAGGCC601.546141E-1037.4999965
Produced by FastQC (version 0.11.5)