##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934716.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	437216
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.98091332430652	33.0	31.0	34.0	30.0	34.0
2	32.181658493742226	33.0	31.0	34.0	30.0	34.0
3	31.932260027080435	33.0	31.0	34.0	30.0	34.0
4	35.760685793749545	37.0	35.0	37.0	35.0	37.0
5	32.10648512405768	37.0	35.0	37.0	0.0	37.0
6	33.970666672765866	37.0	35.0	37.0	19.0	37.0
7	20.596082942984705	32.0	0.0	37.0	0.0	37.0
8	28.012344013027885	35.0	17.0	37.0	17.0	37.0
9	34.82969058771866	37.0	32.0	39.0	32.0	39.0
10	36.23172070555515	37.0	35.0	39.0	32.0	39.0
11	36.694357022615826	38.0	35.0	39.0	33.0	39.0
12	37.022725609309816	39.0	37.0	39.0	34.0	39.0
13	36.78854616482471	39.0	37.0	39.0	33.0	39.0
14	38.05853628412501	40.0	37.0	41.0	33.0	41.0
15	38.26995809851424	40.0	38.0	41.0	33.0	41.0
16	38.36633380297153	40.0	38.0	41.0	34.0	41.0
17	38.35628385054527	40.0	38.0	41.0	34.0	41.0
18	38.24077801361341	40.0	37.0	41.0	34.0	41.0
19	38.17872859181732	40.0	37.0	41.0	34.0	41.0
20	38.10436946497841	40.0	37.0	41.0	34.0	41.0
21	38.01177907487374	40.0	37.0	41.0	33.0	41.0
22	37.98811342677303	40.0	36.0	41.0	34.0	41.0
23	37.9477192051526	40.0	36.0	41.0	34.0	41.0
24	37.81738774427286	40.0	36.0	41.0	33.0	41.0
25	37.66901257044573	40.0	35.0	41.0	33.0	41.0
26	37.541311388421285	39.0	35.0	41.0	33.0	41.0
27	37.49123545341433	39.0	35.0	41.0	33.0	41.0
28	37.38654349337627	39.0	35.0	41.0	33.0	41.0
29	37.33170332284271	39.0	35.0	41.0	33.0	41.0
30	37.146561425016465	39.0	35.0	41.0	32.0	41.0
31	37.01085275927688	39.0	35.0	41.0	32.0	41.0
32	36.90475417185098	39.0	35.0	41.0	31.0	41.0
33	36.70354698821635	39.0	35.0	41.0	31.0	41.0
34	36.49835550391568	39.0	35.0	41.0	30.0	41.0
35	36.29920222498719	39.0	35.0	41.0	30.0	41.0
36	36.10124972553612	39.0	35.0	41.0	29.0	41.0
37	36.03459159774574	39.0	35.0	40.0	29.0	41.0
38	35.85930524043036	39.0	35.0	40.0	28.0	41.0
39	35.74800556246798	39.0	35.0	40.0	27.0	41.0
40	35.62003220376199	39.0	35.0	40.0	26.0	41.0
41	35.482640159555004	39.0	35.0	40.0	26.0	41.0
42	35.40621111761692	38.0	35.0	40.0	26.0	41.0
43	35.35519285662007	38.0	34.0	40.0	25.0	41.0
44	35.19952609236624	38.0	34.0	40.0	25.0	41.0
45	35.09089100124424	38.0	34.0	40.0	24.0	41.0
46	35.00499981702408	38.0	34.0	40.0	24.0	41.0
47	34.93870764107444	38.0	34.0	40.0	24.0	41.0
48	34.788443240869505	38.0	34.0	40.0	23.0	41.0
49	34.68251161897094	38.0	34.0	40.0	24.0	41.0
50	34.51672857351973	37.0	34.0	40.0	23.0	41.0
51	32.659522981775595	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	12.0
9	23.0
10	17.0
11	21.0
12	7.0
13	16.0
14	12.0
15	32.0
16	38.0
17	81.0
18	155.0
19	300.0
20	500.0
21	894.0
22	1473.0
23	2397.0
24	3595.0
25	5001.0
26	6002.0
27	6669.0
28	7153.0
29	7728.0
30	9139.0
31	11670.0
32	15723.0
33	23027.0
34	31392.0
35	39280.0
36	50971.0
37	81941.0
38	80996.0
39	50939.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.804160872429186	19.14728646710093	23.17138439581351	18.877168264656373
2	33.54337444192344	24.130864378247825	25.287729634780064	17.03803154504867
3	32.03382309888019	23.022259020712873	29.15286723267218	15.791050647734759
4	28.181722535314353	24.740402912976652	29.62929078533265	17.448583766376345
5	22.069411915391935	35.96689050720926	25.575687989460587	16.388009587938228
6	26.157093976432698	33.39836602503111	27.351469296640563	13.093070701895632
7	48.14874112566786	43.09929188318817	6.471629583546805	2.2803374075971603
8	83.94752250603821	3.933296128229525	9.30249579155383	2.8166855741784382
9	78.40289467906022	6.040263851277173	11.194466808168045	4.362374661494547
10	42.270639683817606	24.29279806777428	19.828871770475008	13.607690477933104
11	31.78543328697943	24.725993559247602	26.214502671448436	17.27407048232453
12	29.350938666471492	21.339109273219645	30.44307619117324	18.866875869135622
13	25.216140305935742	22.62863207201932	31.9620050501354	20.193222571909537
14	21.12822952499451	23.51217704750055	33.377552514089146	21.982040913415794
15	20.65569421064188	24.63633535826685	34.576502232306225	20.13146819878504
16	25.569055112347215	23.62470723852741	31.191905145282885	19.614332503842494
17	24.84790126619337	24.516943570226157	29.262195345092586	21.37295981848789
18	25.209964868623292	23.80196516138476	31.193506184586106	19.794563785405842
19	24.679105979653077	25.436168850179314	28.504675034765427	21.380050135402183
20	25.286586035277757	26.57908219278343	29.517218034106712	18.6171137378321
21	25.317234501939545	26.101972480421576	30.30767401010027	18.273119007538607
22	25.272176681548707	22.705710678474713	31.08943862987631	20.93267401010027
23	23.833985947449314	24.317957256825	31.00389738710386	20.844159408621827
24	23.003503988875064	24.225096977237794	32.021700944155754	20.749698089731393
25	22.96073336748884	25.587581424284565	30.089017785259458	21.36266742296714
26	22.227457366610555	26.0077398814316	29.528654029129765	22.236148722828077
27	22.49643196955281	25.532459928273436	30.947174851789505	21.02393325038425
28	20.95348752104223	25.621431969552805	30.9172125448291	22.507867964575862
29	23.06891788040694	24.905309961209106	30.14528288077289	21.88048927761107
30	23.07760923662446	25.150040254702482	30.587169728463735	21.185180780209325
31	24.296915025982578	25.09194539998536	29.66245517089951	20.948684403132546
32	25.403004464612454	24.424998170240798	29.00259825806924	21.169399107077506
33	24.33648356876235	25.03773878357608	29.375182975920367	21.2505946717412
34	22.59501024665154	24.254830564297738	31.70995571982727	21.44020346922345
35	23.058396764985726	26.12758910927322	30.177761106638368	20.636253019102686
36	24.227384176242406	27.025543438483496	28.52708958501061	20.219982800263487
37	23.256696918685503	27.02485727878211	28.721501500402546	20.99694430212984
38	22.824873746614944	26.608587059942913	29.322119959013392	21.24441923442875
39	22.602558003366756	27.29314572202298	28.373389811900758	21.730906462709505
40	24.657835028910196	25.37944631486496	28.551105174559027	21.41161348166581
41	21.395831808534	25.059695894020344	29.58857864305057	23.955893654395084
42	21.37158749908512	25.25593756861597	29.714374588304178	23.658100343994732
43	21.70208775525141	25.554645758618165	29.84383005196516	22.899436434165263
44	22.568478738198053	25.97732013467028	28.71395374368733	22.74024738344434
45	21.629126107004318	25.87919929737247	28.514509990485255	23.97716460513796
46	22.431475517821855	26.45191392812706	28.279614652711704	22.83699590133938
47	21.0218747712801	25.576374149161968	30.912180707018955	22.489570372538974
48	22.083821269120985	25.99996340481593	30.075523311132258	21.840692014930834
49	22.364918026787674	25.32615457805753	29.79145319475957	22.517474200395228
50	21.526888311498208	25.789541096391716	30.27839786284125	22.405172729268827
51	21.687220961721437	25.793886774500475	28.995736661055403	23.52315560272268
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	271.0
1	578.0
2	885.0
3	4804.5
4	8724.0
5	5685.0
6	2646.0
7	2275.5
8	1905.0
9	1855.5
10	1806.0
11	1823.0
12	1840.0
13	1809.0
14	1778.0
15	1746.0
16	1714.0
17	1648.5
18	1583.0
19	1552.5
20	1522.0
21	1640.0
22	1758.0
23	2012.5
24	2267.0
25	2738.5
26	4021.0
27	4832.0
28	5600.5
29	6369.0
30	7293.5
31	8218.0
32	9752.0
33	11286.0
34	12402.0
35	13518.0
36	14495.5
37	15473.0
38	16771.0
39	18069.0
40	19370.0
41	20671.0
42	22465.5
43	24260.0
44	26600.0
45	28940.0
46	29393.0
47	29846.0
48	29992.5
49	30139.0
50	28774.0
51	27409.0
52	26162.5
53	24916.0
54	24791.0
55	24666.0
56	24629.5
57	24593.0
58	23495.0
59	22397.0
60	20623.5
61	18850.0
62	16961.5
63	15073.0
64	13246.0
65	11419.0
66	9879.5
67	8340.0
68	7061.0
69	5782.0
70	4874.5
71	3967.0
72	3263.5
73	2560.0
74	2117.5
75	1305.5
76	936.0
77	741.5
78	547.0
79	416.0
80	285.0
81	205.0
82	125.0
83	97.5
84	70.0
85	53.0
86	36.0
87	29.5
88	23.0
89	15.5
90	8.0
91	5.5
92	3.0
93	3.5
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	437216.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.48259489899528
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.4927290103078	32.30202371011794
2	9.696766133124374	9.014617039926344
3	5.511734502624482	7.685991662289271
4	3.794188231727355	7.054548581836715
5	2.9083809656880684	6.759454712001359
6	2.238782113195815	6.243864122087859
7	1.7095294771385816	5.562435630760673
8	1.2780532639664024	4.752578570263117
9	0.9673682221102249	4.046920666785978
>10	2.3790210984455995	14.03064490250006
>50	0.014589233039932124	0.46234985225694525
>100	0.007294616519959303	0.6919505122562346
>500	5.210440371399502E-4	0.16178611907146123
>1k	0.0010420880742799004	1.230833917846057
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3222	0.7369355192856619	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1860	0.4254190148576448	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	668	0.15278489350801433	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02447302934933763	0.0
2	0.0	0.0	0.0	0.1022377955061114	0.0
3	0.0	0.0	0.0	0.14592329649418137	0.0
4	0.0	0.0	0.0	0.2017309522066896	0.0
5	0.0	0.0	0.0	0.42290309595257264	0.0
6	0.0	0.0	0.0	0.6616866720339604	0.0
7	0.0	0.0	0.0	0.8638750640415721	0.0
8	0.0	0.0	0.0	1.359053648539852	0.0
9	0.0	0.0	0.0	1.6216240942691942	0.0
10	0.0	0.0	0.0	2.1129144404596354	0.0
11	0.0	0.0	0.0	2.542679133426041	0.0
12	0.0	0.0	0.0	2.9207531288882382	0.0
13	0.0	0.0	0.0	3.101441850252507	0.0
14	0.0	0.0	0.0	3.2336419527190223	0.0
15	0.0	0.0	0.0	3.348230622850033	0.0
16	0.0	0.0	0.0	3.5161110297884797	0.0
17	0.0	0.0	0.0	3.7018315889628925	0.0
18	0.0	0.0	0.0	3.9513650003659517	0.0
19	0.0	0.0	0.0	4.120389006806704	0.0
20	0.0	0.0	0.0	4.289870453048379	0.0
21	0.0	0.0	0.0	4.47536229232233	0.0
22	0.0	0.0	0.0	4.683039961941009	0.0
23	0.0	0.0	0.0	4.894377149967064	0.0
24	0.0	0.0	0.0	5.080326429041938	0.0
25	0.0	0.0	0.0	5.250265315084535	0.0
26	0.0	0.0	0.0	5.411055405108688	0.0
27	0.0	0.0	0.0	5.612328917514455	0.0
28	0.0	0.0	0.0	5.796219717485179	0.0
29	0.0	0.0	0.0	6.0087005050135405	0.0
30	0.0	0.0	0.0	6.3458336382932	0.0
31	0.0	0.0	0.0	6.580042816365366	0.0
32	0.0	0.0	0.0	6.801901119812633	0.0
33	0.0	0.0	0.0	7.014381907340994	0.0
34	0.0	0.0	0.0	7.220915977457366	0.0
35	0.0	0.0	0.0	7.48165666398302	0.0
36	0.0	0.0	0.0	7.707174485837664	0.0
37	0.0	0.0	0.0	7.94572934201859	0.0
38	0.0	0.0	0.0	8.193432994217961	0.0
39	0.0	0.0	0.0	8.648356876235088	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGAT	20	7.028485E-4	45.000004	35
CGGGTAT	25	3.8868267E-5	45.0	6
ACGTTGA	25	3.8868267E-5	45.0	1
CGTTAAG	25	3.8868267E-5	45.0	2
CGGTAAG	30	2.1624055E-6	44.999996	2
CGTTATT	265	0.0	44.150944	1
CGTTTTA	560	0.0	42.99107	1
TTAATCG	95	0.0	42.63158	20
AGGCGAT	235	0.0	41.17021	7
CGTTTTT	1105	0.0	40.723984	1
TTACACG	135	0.0	40.000004	34
CTATACG	40	3.451678E-7	39.375004	1
CGAGATA	160	0.0	39.375004	19
CTAAGGC	75	0.0	39.0	4
TAACGGG	75	0.0	39.0	3
CTTACAC	150	0.0	39.0	33
TCGTTGA	105	0.0	38.57143	24
ACACGCG	140	0.0	38.571426	36
GTCTTAC	155	0.0	37.741936	31
TTAGGCC	60	1.546141E-10	37.499996	5
>>END_MODULE