##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934715.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	400455
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.891401031326865	31.0	31.0	34.0	30.0	34.0
2	32.07597857437165	33.0	31.0	34.0	30.0	34.0
3	31.73925659562248	33.0	31.0	34.0	30.0	34.0
4	35.62682698430535	37.0	35.0	37.0	33.0	37.0
5	32.04947122647988	37.0	35.0	37.0	0.0	37.0
6	33.92644117316552	37.0	35.0	37.0	19.0	37.0
7	20.870075289358354	32.0	0.0	37.0	0.0	37.0
8	28.15277122273414	35.0	17.0	37.0	17.0	37.0
9	34.88124758087675	37.0	32.0	39.0	32.0	39.0
10	36.25412343459315	37.0	35.0	39.0	32.0	39.0
11	36.664231936172605	38.0	35.0	39.0	33.0	39.0
12	36.98554394376397	39.0	37.0	39.0	33.0	39.0
13	36.779675618983404	39.0	37.0	39.0	33.0	39.0
14	38.100568103781946	40.0	37.0	41.0	33.0	41.0
15	38.25915271378807	40.0	38.0	41.0	33.0	41.0
16	38.337338777141	40.0	38.0	41.0	34.0	41.0
17	38.287338152851135	40.0	37.0	41.0	34.0	41.0
18	38.154222072392656	40.0	37.0	41.0	33.0	41.0
19	38.05579153712652	40.0	37.0	41.0	34.0	41.0
20	38.000791599555505	40.0	36.0	41.0	34.0	41.0
21	37.88000399545517	40.0	36.0	41.0	33.0	41.0
22	37.85618359116505	40.0	36.0	41.0	33.0	41.0
23	37.77661909577855	39.0	36.0	41.0	33.0	41.0
24	37.624399745289736	39.0	35.0	41.0	33.0	41.0
25	37.46098812600667	39.0	35.0	41.0	33.0	41.0
26	37.33587793884456	39.0	35.0	41.0	33.0	41.0
27	37.29096153125819	39.0	35.0	41.0	33.0	41.0
28	37.191399782747126	39.0	35.0	41.0	33.0	41.0
29	37.13565069733178	39.0	35.0	41.0	32.0	41.0
30	36.92600666741581	39.0	35.0	41.0	32.0	41.0
31	36.770403665830116	39.0	35.0	41.0	31.0	41.0
32	36.587279469603324	39.0	35.0	41.0	31.0	41.0
33	36.326001673096854	39.0	35.0	41.0	30.0	41.0
34	36.00966900151078	39.0	35.0	40.0	29.0	41.0
35	35.76837347517199	39.0	35.0	40.0	27.0	41.0
36	35.53448202669463	39.0	35.0	40.0	26.0	41.0
37	35.429513927907	39.0	35.0	40.0	25.0	41.0
38	35.26914884319087	38.0	34.0	40.0	24.0	41.0
39	35.15795033149792	38.0	34.0	40.0	24.0	41.0
40	35.02384787304441	38.0	34.0	40.0	23.0	41.0
41	34.86244646714363	38.0	34.0	40.0	23.0	41.0
42	34.74415602252438	38.0	34.0	40.0	23.0	41.0
43	34.6448864416726	38.0	34.0	40.0	22.0	41.0
44	34.49815584772321	38.0	34.0	40.0	22.0	41.0
45	34.35755578029991	38.0	33.0	40.0	22.0	41.0
46	34.22118090671861	37.0	33.0	40.0	21.0	41.0
47	34.15249653519122	37.0	33.0	40.0	21.0	41.0
48	34.048389956424565	37.0	33.0	40.0	20.0	41.0
49	33.92500280930442	37.0	33.0	40.0	20.0	41.0
50	33.775720118365356	37.0	33.0	40.0	20.0	41.0
51	31.98612578192306	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	9.0
9	18.0
10	15.0
11	21.0
12	8.0
13	18.0
14	24.0
15	35.0
16	54.0
17	102.0
18	185.0
19	356.0
20	606.0
21	973.0
22	1785.0
23	2792.0
24	4191.0
25	6016.0
26	6872.0
27	7372.0
28	7612.0
29	8152.0
30	9292.0
31	11502.0
32	14938.0
33	21212.0
34	28614.0
35	35843.0
36	45536.0
37	71741.0
38	70376.0
39	44177.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.73532606659924	17.9720567854066	22.082880723177386	22.20973642481677
2	32.9395312831654	23.585671298897502	25.884306601241086	17.590490816696008
3	31.162053164525354	22.290394676055985	30.5934499506811	15.954102208737561
4	26.448664643967483	24.923649348865666	30.548501080021474	18.079184927145374
5	21.82792074015807	35.80602065150891	26.23191120100885	16.134147407324168
6	25.70625913023935	32.7320173302868	28.056086201945284	13.505637337528562
7	47.56239777253374	42.315865702763105	7.900263450325255	2.221473074377895
8	82.41475321821427	3.9262838521182153	11.195764817520072	2.4631981121474325
9	77.20992371177785	5.990685595135534	12.793447453521619	4.005943239564995
10	41.41863630120738	25.598132124708144	20.253211971382555	12.730019602701926
11	30.771996853579054	25.437065338177824	27.020514165137154	16.770423643105968
12	27.3426477381978	22.95588767776654	31.077649174064504	18.623815409971158
13	23.842379293553584	23.828644916407587	32.88384462673709	19.445131163301742
14	20.675231923686805	24.685170618421544	34.17662409009751	20.462973367794135
15	20.40528898378095	24.446941603925534	35.05812138692237	20.08964802537114
16	24.37352511518148	23.589916470015357	32.97424179995255	19.06231661485061
17	24.852230587706483	23.85811139828445	31.085390368455883	20.204267645553184
18	25.76419323020065	22.90844164762583	32.01932801438364	19.30803710778989
19	24.329075676418075	25.541696320435502	29.85454046022649	20.274687542919928
20	26.330299284563807	25.579902860496183	30.091271179033853	17.998526675906156
21	24.79654393127817	25.577405701015092	31.342597795008174	18.283452572698554
22	25.64907417812239	21.788465620356845	32.10348228889638	20.45897791262439
23	23.207851069408548	24.52959758274962	31.931927432545482	20.33062391529635
24	22.186263125694524	24.217452647613342	33.54933762844764	20.046946598244496
25	23.078747924236183	24.801038818344136	31.074153150790973	21.046060106628712
26	22.461949532406887	25.19933575557803	31.10461849646028	21.234096215554807
27	21.6019278071194	25.35016418823588	31.86475384250415	21.183154162140568
28	20.66649186550299	26.87917493850745	31.338602339838435	21.115730856151128
29	22.29414041527762	25.698268219899862	30.941803698293192	21.065787666529324
30	22.879974029541398	24.47615836985429	32.10223370915584	20.541633891448477
31	24.06537563521494	24.84274138167834	30.534017555031152	20.557865428075566
32	24.09683984467668	24.55332059781998	30.628160467468255	20.721679090035085
33	24.132549225256273	25.095453921164673	29.614313718145606	21.157683135433444
34	22.248442396773672	25.39661135458416	31.666978811601805	20.687967437040367
35	22.172279032600418	27.104169007753683	30.27456268494587	20.44898927470003
36	23.21259567242262	28.58573372788453	27.99865153388021	20.20301906581264
37	22.770598444269645	27.76341911076151	28.55476895031901	20.911213494649836
38	22.391529635040143	27.55590515788291	29.210023598157097	20.842541608919856
39	22.732391904208963	26.22691688204667	28.329025733228452	22.711665480515915
40	24.470414903047782	25.128416426315063	28.88139741044562	21.51977126019153
41	21.05280243722765	25.718744927644803	29.43202107602602	23.79643155910152
42	22.298635302343584	26.12752993469928	28.894882071643508	22.67895269131363
43	22.41500293416239	25.70975515351288	29.335131288159715	22.540110624165013
44	22.575820004744603	26.15499868899127	28.900375822501907	22.36880548376222
45	22.459951804822015	26.45290981508534	27.866551797330537	23.22058658276211
46	22.016955712876605	26.894907043238312	28.426165237042866	22.66197200684222
47	21.07702488419423	27.099923836635824	30.115993057896645	21.7070582212733
48	21.88485597632693	26.848709592838148	29.94743479292305	21.318999637911876
49	22.78682998089673	26.256383363923536	29.32239577480616	21.634390880373573
50	21.5382502403516	26.70212633129815	29.719943564195727	22.039679864154525
51	21.47931727659787	26.328551272927047	28.384462673708654	23.807668776766427
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	335.0
1	750.0
2	1165.0
3	5458.5
4	9752.0
5	6625.5
6	3499.0
7	3044.5
8	2590.0
9	2552.0
10	2514.0
11	2459.0
12	2404.0
13	2372.0
14	2340.0
15	2244.0
16	2148.0
17	2110.5
18	2073.0
19	2102.0
20	2131.0
21	2177.5
22	2224.0
23	2598.0
24	2972.0
25	3288.5
26	4217.0
27	4829.0
28	5700.5
29	6572.0
30	7168.5
31	7765.0
32	8808.0
33	9851.0
34	11331.5
35	12812.0
36	13367.5
37	13923.0
38	14656.0
39	15389.0
40	16753.0
41	18117.0
42	18969.5
43	19822.0
44	20611.5
45	21401.0
46	22446.0
47	23491.0
48	24428.5
49	25366.0
50	25451.5
51	25537.0
52	25267.5
53	24998.0
54	24151.0
55	23304.0
56	22295.0
57	21286.0
58	20544.5
59	19803.0
60	18445.0
61	17087.0
62	15499.0
63	13911.0
64	12387.5
65	10864.0
66	9357.5
67	7851.0
68	6571.5
69	5292.0
70	4645.0
71	3998.0
72	3447.0
73	2896.0
74	2428.0
75	1580.0
76	1200.0
77	919.0
78	638.0
79	502.0
80	366.0
81	271.0
82	176.0
83	134.0
84	92.0
85	74.5
86	57.0
87	39.0
88	21.0
89	18.0
90	15.0
91	11.0
92	7.0
93	5.5
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	400455.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.137937274813126
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.9723892186327	32.74519642012899
2	9.574027073783983	8.83451721195216
3	5.094457217342399	7.05143242628888
4	3.423604389338549	6.318321782763076
5	2.4535224276241716	5.660023193403562
6	1.9046870854613935	5.272699996629864
7	1.4927798153412286	4.8211647039724514
8	1.1775968742326064	4.3465512574688
9	0.9016118932842558	3.7438661680717056
>10	2.97978928537776	18.651500769473245
>50	0.01887348838591628	0.6325988363431193
>100	0.004995923396271931	0.36163139956132845
>500	5.551025995857701E-4	0.19387745713025895
>1k	0.0011102051991715401	1.3666183768124989
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3407	0.8507822352074516	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1929	0.48170206390231113	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	757	0.18903497271853267	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01498295688654156	0.0
2	0.0	0.0	0.0	0.058683247805621105	0.0
3	0.0	0.0	0.0	0.09688978786630208	0.0
4	0.0	0.0	0.0	0.14208837447403577	0.0
5	0.0	0.0	0.0	0.2801812937783272	0.0
6	0.0	0.0	0.0	0.43675319324268647	0.0
7	0.0	0.0	0.0	0.5718495211696695	0.0
8	0.0	0.0	0.0	0.9254473036920503	0.0
9	0.0	0.0	0.0	1.133960120363087	0.0
10	0.0	0.0	0.0	1.5020414278757912	0.0
11	0.0	0.0	0.0	1.8399071056673035	0.0
12	0.0	0.0	0.0	2.142562834775443	0.0
13	0.0	0.0	0.0	2.286399220886242	0.0
14	0.0	0.0	0.0	2.3865353160779614	0.0
15	0.0	0.0	0.0	2.4829256720480455	0.0
16	0.0	0.0	0.0	2.6190208637674646	0.0
17	0.0	0.0	0.0	2.78932714037782	0.0
18	0.0	0.0	0.0	2.9723689303417364	0.0
19	0.0	0.0	0.0	3.0979760522405764	0.0
20	0.0	0.0	0.0	3.248804484898428	0.0
21	0.0	0.0	0.0	3.4313468429661262	0.0
22	0.0	0.0	0.0	3.6158869286186963	0.0
23	0.0	0.0	0.0	3.8096665043513003	0.0
24	0.0	0.0	0.0	3.97647675768813	0.0
25	0.0	0.0	0.0	4.12256058733191	0.0
26	0.0	0.0	0.0	4.267645553183254	0.0
27	0.0	0.0	0.0	4.433207226779539	0.0
28	0.0	0.0	0.0	4.59677117279095	0.0
29	0.0	0.0	0.0	4.771572336467268	0.0
30	0.0	0.0	0.0	5.00455731605299	0.0
31	2.49715948109026E-4	0.0	0.0	5.200084903422357	0.0
32	2.49715948109026E-4	0.0	0.0	5.419085789913973	0.0
33	2.49715948109026E-4	0.0	0.0	5.606123035047633	0.0
34	2.49715948109026E-4	0.0	0.0	5.782921926308823	0.0
35	2.49715948109026E-4	0.0	0.0	5.987689003758225	0.0
36	2.49715948109026E-4	0.0	0.0	6.181968011387047	0.0
37	2.49715948109026E-4	0.0	0.0	6.387484236680776	0.0
38	2.49715948109026E-4	0.0	0.0	6.646439674869836	0.0
39	2.49715948109026E-4	0.0	0.0	7.038493713401007	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAATCGA	20	7.027774E-4	45.000004	22
TAGCCGT	20	7.027774E-4	45.000004	44
TGCGAAA	20	7.027774E-4	45.000004	1
TCCGGTA	20	7.027774E-4	45.000004	18
CGGGTAT	25	3.8862374E-5	45.0	6
ACTGCGC	25	3.8862374E-5	45.0	27
ACGGGTA	25	3.8862374E-5	45.0	5
TTAACGG	25	3.8862374E-5	45.0	2
ACGTCAT	25	3.8862374E-5	45.0	35
TACGGTT	30	2.1619471E-6	44.999996	33
CGTTTTA	685	0.0	44.014595	1
CACGACC	160	0.0	42.187504	27
CGGTTTA	60	3.6379788E-12	41.249996	1
ACGGGAA	60	3.6379788E-12	41.249996	5
CGTTTTT	1485	0.0	41.060608	1
CAGGCGA	45	1.9228537E-8	40.0	6
GCGAGAC	175	0.0	39.857143	21
TAACGGG	85	0.0	39.705883	3
CGGGTCT	40	3.4506957E-7	39.375004	6
CTCACTA	80	0.0	39.375004	45
>>END_MODULE