##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934714.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	385611
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.933565173192672	31.0	31.0	34.0	30.0	34.0
2	32.125880745103224	33.0	31.0	34.0	30.0	34.0
3	32.012043224908005	33.0	31.0	34.0	30.0	34.0
4	35.784565274330866	37.0	35.0	37.0	35.0	37.0
5	32.135494060076084	37.0	35.0	37.0	10.0	37.0
6	33.98350669457044	37.0	35.0	37.0	19.0	37.0
7	20.61488909808071	32.0	0.0	37.0	0.0	37.0
8	28.02146463664159	35.0	17.0	37.0	17.0	37.0
9	34.78241543939359	37.0	32.0	39.0	32.0	39.0
10	36.1461083838376	37.0	35.0	39.0	32.0	39.0
11	36.58291127587128	38.0	35.0	39.0	32.0	39.0
12	36.919621068901044	39.0	37.0	39.0	33.0	39.0
13	36.660419438242165	39.0	35.0	39.0	33.0	39.0
14	37.946658679342654	40.0	37.0	41.0	33.0	41.0
15	38.15556610158943	40.0	37.0	41.0	33.0	41.0
16	38.252645801079325	40.0	37.0	41.0	34.0	41.0
17	38.25805280451024	40.0	37.0	41.0	34.0	41.0
18	38.16255241681384	40.0	37.0	41.0	34.0	41.0
19	38.119524598624004	40.0	37.0	41.0	34.0	41.0
20	38.10183319459248	40.0	37.0	41.0	34.0	41.0
21	38.00571041801194	40.0	37.0	41.0	33.0	41.0
22	37.99936983125481	40.0	37.0	41.0	34.0	41.0
23	37.93493442873777	40.0	36.0	41.0	34.0	41.0
24	37.834294146173214	40.0	36.0	41.0	33.0	41.0
25	37.69485050996989	40.0	36.0	41.0	33.0	41.0
26	37.56933282504908	39.0	36.0	41.0	33.0	41.0
27	37.55171403305403	39.0	36.0	41.0	33.0	41.0
28	37.44633581510901	39.0	36.0	41.0	33.0	41.0
29	37.43447152700519	39.0	36.0	41.0	33.0	41.0
30	37.29181221490051	39.0	36.0	41.0	32.0	41.0
31	37.155003358306686	39.0	35.0	41.0	32.0	41.0
32	37.08444261185495	39.0	35.0	41.0	32.0	41.0
33	36.96214578940953	39.0	35.0	41.0	31.0	41.0
34	36.811245011164104	39.0	35.0	41.0	31.0	41.0
35	36.667530231243404	39.0	35.0	41.0	31.0	41.0
36	36.52267959160916	39.0	35.0	41.0	30.0	41.0
37	36.4569916314628	39.0	35.0	41.0	30.0	41.0
38	36.309340241849945	39.0	35.0	41.0	30.0	41.0
39	36.25024960387541	39.0	35.0	40.0	30.0	41.0
40	36.12274805438641	39.0	35.0	40.0	29.0	41.0
41	35.973198378676955	39.0	35.0	40.0	29.0	41.0
42	35.88302200922691	39.0	35.0	40.0	28.0	41.0
43	35.816288954412606	39.0	35.0	40.0	28.0	41.0
44	35.68325851700288	38.0	35.0	40.0	28.0	41.0
45	35.588351992033424	38.0	35.0	40.0	27.0	41.0
46	35.42951834880229	38.0	34.0	40.0	27.0	41.0
47	35.38127802370783	38.0	34.0	40.0	27.0	41.0
48	35.24905150527345	38.0	34.0	40.0	26.0	41.0
49	35.11680424054293	38.0	34.0	40.0	26.0	41.0
50	34.94861661104066	38.0	34.0	40.0	25.0	41.0
51	32.96720529238014	36.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	10.0
9	27.0
10	13.0
11	13.0
12	13.0
13	12.0
14	12.0
15	11.0
16	46.0
17	72.0
18	136.0
19	235.0
20	446.0
21	704.0
22	1126.0
23	1618.0
24	2456.0
25	3479.0
26	4239.0
27	5022.0
28	5499.0
29	6421.0
30	8114.0
31	10225.0
32	13955.0
33	20383.0
34	28453.0
35	36049.0
36	44564.0
37	73429.0
38	72916.0
39	45906.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.2677257650845	19.070773395987146	22.74079318276709	15.920707656161262
2	32.01386889896813	24.529642567250416	25.352492537816605	18.103995995964844
3	31.251701844605055	24.176955532907517	27.85812645386153	16.713216168625895
4	27.682042265391804	25.449481472260903	27.22847636607877	19.63999989626852
5	22.295266473207455	37.454585061110805	22.902614292642067	17.34753417303967
6	26.40614505291602	34.78038748894611	25.292328279016935	13.521139179120928
7	48.58471361034825	43.440410154274645	5.1909826223836975	2.7838936129934053
8	84.03390982103727	4.468233530682475	8.074977114242072	3.422879534038189
9	78.8284047913571	6.542863144464239	9.995306150498818	4.633425913679848
10	43.40955003876964	24.03536206176691	18.471724095007662	14.083363804455788
11	32.362406674083466	25.29933015396345	24.261496689669123	18.07676648228396
12	29.010583204317303	21.996001151419435	29.583180977721067	19.410234666542188
13	24.530679882057306	25.303998070594456	29.941054586098424	20.22426746124981
14	20.565025375313464	27.49195432702905	31.00819219368742	20.934828103970062
15	20.043774684850796	24.843170967633185	35.00600345944488	20.10705088807114
16	23.053802925746414	23.810783406075036	32.58102076963572	20.55439289854283
17	23.017756236206953	23.828936415195624	28.724024988913698	24.429282359683725
18	24.11938456112507	23.939410442129503	30.89460622233287	21.046598774412555
19	24.70054018168569	26.081465518359177	27.779549857239548	21.438444442715586
20	26.315120678611347	25.4642632082591	28.336847237241674	19.883768875887878
21	24.17254694497823	26.29956095650798	29.49941780706463	20.028474291449154
22	24.054293056992666	24.010985163804975	29.518608130992114	22.416113648210242
23	21.518577011547908	26.119586837512415	29.947537803641495	22.414298347298185
24	22.039049715905406	25.145288905140156	31.44490172738848	21.370759651565958
25	21.297628957680146	26.133071930001993	30.136329098495633	22.43297001382222
26	20.83265259549131	27.282416736037096	29.005655958984573	22.87927470948702
27	21.392024605107217	26.682070791548995	30.223204213572746	21.70270038977104
28	19.575945706943006	26.382027483655808	30.995225758601286	23.0468010507999
29	20.69520838357827	25.10561161377658	31.325351195894306	22.873828806750847
30	21.485901595130844	25.669651540023498	30.336530856225576	22.507916008620086
31	22.924138574885053	26.312008734190673	28.95586484825381	21.807987842670464
32	23.63781116202598	25.4813789025728	28.933821908607378	21.946988026793843
33	22.67777630824847	25.786868113202168	28.005684485141764	23.529671093407607
34	20.582400398328886	24.89711133759151	31.085731475502516	23.434756788577086
35	21.215162430532324	25.622453716309963	30.594303585737958	22.568080267419756
36	23.69590079121187	25.45544603240053	28.680717095726006	22.167936080661597
37	21.131399259875884	26.48498097823973	30.332381596998008	22.051238164886374
38	21.055675278972853	27.815337218077286	28.364336079624287	22.76465142332558
39	21.543472566913287	26.289965794544244	28.810381446587364	23.356180191955104
40	23.810005419969865	24.606922520363785	29.17136699938539	22.411705060280955
41	20.62181836099074	24.645043839517026	29.657348986413766	25.075788813078464
42	21.87982189304766	24.594734071382817	28.665935359727808	24.859508675841717
43	21.224238935092618	25.070342910342287	29.76341442541836	23.94200372914673
44	20.933790789163172	25.329412283363283	29.70065687960146	24.036140047872077
45	21.511056479197947	26.062793851835142	28.13872010912552	24.287429559841396
46	21.923389114937073	26.512210491920612	28.42294436621362	23.14145602692869
47	20.631932180357925	25.796981932569352	29.905526553962414	23.665559333110313
48	21.25847032372002	25.311259274242698	30.205051204452154	23.22521919758513
49	21.16744594941534	24.028100858118673	31.02038064266839	23.784072549797592
50	20.27353991457713	25.834843923020866	30.299706180580948	23.591909981821058
51	20.377271395266213	25.481638231274523	28.131977562880728	26.00911281057854
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	194.0
1	607.0
2	1020.0
3	2234.5
4	3449.0
5	2376.5
6	1304.0
7	1201.5
8	1099.0
9	1159.5
10	1220.0
11	1202.0
12	1184.0
13	1171.0
14	1158.0
15	1116.0
16	1074.0
17	1109.0
18	1144.0
19	1130.0
20	1116.0
21	1275.5
22	1435.0
23	1681.5
24	1928.0
25	2294.5
26	3208.0
27	3755.0
28	4487.5
29	5220.0
30	6103.0
31	6986.0
32	8094.5
33	9203.0
34	10590.5
35	11978.0
36	13092.0
37	14206.0
38	15618.5
39	17031.0
40	18671.0
41	20311.0
42	22468.0
43	24625.0
44	26382.5
45	28140.0
46	29550.5
47	30961.0
48	31168.5
49	31376.0
50	30389.0
51	29402.0
52	27027.0
53	24652.0
54	23228.0
55	21804.0
56	20468.0
57	19132.0
58	18047.5
59	16963.0
60	15574.5
61	14186.0
62	12641.0
63	11096.0
64	9911.5
65	8727.0
66	7374.0
67	6021.0
68	4975.0
69	3929.0
70	3201.0
71	2473.0
72	1940.5
73	1408.0
74	1182.5
75	751.0
76	545.0
77	415.5
78	286.0
79	209.5
80	133.0
81	90.5
82	48.0
83	38.5
84	29.0
85	23.5
86	18.0
87	16.5
88	15.0
89	9.0
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	2.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	385611.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.435042762516225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.27776732756955	29.533973372345258
2	8.460662947596832	7.011358620658226
3	4.185194423493038	5.202411297206353
4	2.86329926120028	4.745637093188589
5	2.2092928882558933	4.577107264990295
6	1.8288736675648498	4.546767517367367
7	1.5561181930477894	4.513447671072524
8	1.3239123491457683	4.388509184054257
9	1.1258520207975458	4.198475396341045
>10	5.141756608196672	29.84176206868923
>50	0.019659993188070157	0.5649732953824835
>100	0.0063419332864742455	0.4235985823984016
>500	6.341933286474244E-4	0.1742219975993426
>1k	6.341933286474244E-4	0.27775663870664424
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1057	0.27411043772091565	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	663	0.17193492924216372	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03604668953945816	0.0
2	0.0	0.0	0.0	0.2305432158314986	0.0
3	0.0	0.0	0.0	0.3135284003827692	0.0
4	0.0	0.0	0.0	0.4157039088615211	0.0
5	0.0	0.0	0.0	0.7214524481926086	0.0
6	0.0	0.0	0.0	0.9934882562997425	0.0
7	0.0	0.0	0.0	1.2424438099535542	0.0
8	0.0	0.0	0.0	1.8243774166193392	0.0
9	0.0	0.0	0.0	2.014984012385539	0.0
10	0.0	0.0	0.0	2.525342897375853	0.0
11	0.0	0.0	0.0	3.2763588175648515	0.0
12	0.0	0.0	0.0	3.8556991372134095	0.0
13	0.0	0.0	0.0	4.119177098163694	0.0
14	0.0	0.0	0.0	4.220055963133832	0.0
15	0.0	0.0	0.0	4.429852882827513	0.0
16	0.0	0.0	0.0	4.86137584249412	0.0
17	0.0	0.0	0.0	5.385738477377461	0.0
18	0.0	0.0	0.0	5.9687093988501365	0.0
19	0.0	0.0	0.0	6.315691201755136	0.0
20	0.0	0.0	0.0	6.6349248335758055	0.0
21	0.0	0.0	0.0	7.057111959980395	0.0
22	0.0	0.0	0.0	7.504453970452087	0.0
23	0.0	0.0	0.0	7.960613156782353	0.0
24	0.0	0.0	0.0	8.32548864010622	0.0
25	0.0	0.0	0.0	8.681287618869794	0.0
26	0.0	0.0	0.0	8.979256297149199	0.0
27	0.0	0.0	0.0	9.3148276371784	0.0
28	0.0	0.0	0.0	9.639247843033523	0.0
29	0.0	0.0	0.0	10.0030860115505	0.0
30	0.0	0.0	0.0	10.406601471431054	0.0
31	0.0	0.0	0.0	10.749174686406768	0.0
32	0.0	0.0	0.0	11.067630332122269	0.0
33	0.0	0.0	0.0	11.418242736851386	0.0
34	0.0	0.0	0.0	11.758222664809873	0.0
35	0.0	0.0	0.0	12.136842569325045	0.0
36	0.0	0.0	0.0	12.485899001843826	0.0
37	0.0	0.0	0.0	12.825100943697146	0.0
38	0.0	0.0	0.0	13.197756288072695	0.0
39	0.0	0.0	0.0	13.629538576441025	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCCG	20	7.02745E-4	45.0	44
TTTCGTC	25	3.885968E-5	45.0	16
CGGTTTA	20	7.02745E-4	45.0	1
ATAGACG	20	7.02745E-4	45.0	22
TTATCGG	20	7.02745E-4	45.0	23
GTCGTGT	20	7.02745E-4	45.0	39
GCTACTA	25	3.885968E-5	45.0	1
TATCGGC	20	7.02745E-4	45.0	24
GCGTACG	25	3.885968E-5	45.0	1
AGGCGAT	215	0.0	41.860462	7
ACGGGCC	65	0.0	41.53846	5
GCGATCA	55	6.002665E-11	40.90909	9
CGTTATT	105	0.0	40.714287	1
TACGGTT	50	1.0768417E-9	40.5	33
TACGGGA	95	0.0	40.263157	4
CACGACC	45	1.9226718E-8	40.0	27
CGTTTTA	215	0.0	39.76744	1
TCAAGCG	40	3.450259E-7	39.375	17
CTCACTA	115	0.0	39.130432	45
CGTTTTT	350	0.0	38.571426	1
>>END_MODULE