##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934712.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	185414
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.88803434476361	31.0	31.0	34.0	30.0	34.0
2	32.06977358775497	33.0	31.0	34.0	30.0	34.0
3	31.80916759252268	33.0	31.0	34.0	30.0	34.0
4	35.67179932475433	37.0	35.0	37.0	35.0	37.0
5	31.991208862329707	37.0	35.0	37.0	0.0	37.0
6	33.898972030159534	37.0	35.0	37.0	19.0	37.0
7	20.853301260961956	32.0	0.0	37.0	0.0	37.0
8	28.11913879210847	35.0	17.0	37.0	17.0	37.0
9	34.78962753621625	37.0	32.0	39.0	31.0	39.0
10	36.28934708274456	37.0	35.0	39.0	32.0	39.0
11	36.66155737970164	38.0	35.0	39.0	33.0	39.0
12	36.882268868585975	39.0	35.0	39.0	33.0	39.0
13	36.5395870861963	39.0	35.0	39.0	32.0	39.0
14	37.92988123874141	40.0	37.0	41.0	33.0	41.0
15	38.1086056069121	40.0	37.0	41.0	33.0	41.0
16	38.2227339898821	40.0	37.0	41.0	34.0	41.0
17	38.180056522161216	40.0	37.0	41.0	34.0	41.0
18	38.076995264650996	40.0	37.0	41.0	34.0	41.0
19	37.97299017334182	40.0	37.0	41.0	34.0	41.0
20	37.93019944556506	40.0	36.0	41.0	34.0	41.0
21	37.83479133183039	40.0	36.0	41.0	33.0	41.0
22	37.79026934319954	39.0	35.0	41.0	33.0	41.0
23	37.73621193653122	39.0	35.0	41.0	33.0	41.0
24	37.56234157075517	39.0	35.0	41.0	33.0	41.0
25	37.43716763566937	39.0	35.0	41.0	33.0	41.0
26	37.2868715415233	39.0	35.0	41.0	33.0	41.0
27	37.23937782475973	39.0	35.0	41.0	33.0	41.0
28	37.14215215679506	39.0	35.0	41.0	33.0	41.0
29	37.14021055583721	39.0	35.0	41.0	33.0	41.0
30	36.99421295047839	39.0	35.0	41.0	32.0	41.0
31	36.85384059456136	39.0	35.0	41.0	32.0	41.0
32	36.69935927168391	39.0	35.0	41.0	31.0	41.0
33	36.49486015079768	39.0	35.0	41.0	31.0	41.0
34	36.26770362540046	39.0	35.0	40.0	30.0	41.0
35	36.03036987498247	39.0	35.0	40.0	29.0	41.0
36	35.85873235030796	39.0	35.0	40.0	29.0	41.0
37	35.73606092312339	38.0	35.0	40.0	28.0	41.0
38	35.64832213317225	38.0	35.0	40.0	27.0	41.0
39	35.570830681609806	38.0	35.0	40.0	27.0	41.0
40	35.42049144077578	38.0	35.0	40.0	26.0	41.0
41	35.30605024431812	38.0	34.0	40.0	26.0	41.0
42	35.18381028401307	38.0	34.0	40.0	25.0	41.0
43	35.059019275782845	38.0	34.0	40.0	24.0	41.0
44	34.88615207050169	38.0	34.0	40.0	24.0	41.0
45	34.7692946595187	38.0	34.0	40.0	23.0	41.0
46	34.66419472100273	38.0	34.0	40.0	23.0	41.0
47	34.57692515128308	38.0	34.0	40.0	23.0	41.0
48	34.49138684241751	37.0	34.0	40.0	23.0	41.0
49	34.36483760665322	37.0	34.0	40.0	23.0	41.0
50	34.21767504071968	37.0	33.0	40.0	22.0	41.0
51	32.37522517177775	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	5.0
9	8.0
10	6.0
11	1.0
12	5.0
13	3.0
14	8.0
15	10.0
16	19.0
17	38.0
18	77.0
19	133.0
20	223.0
21	404.0
22	684.0
23	1054.0
24	1541.0
25	2221.0
26	2780.0
27	3040.0
28	3254.0
29	3628.0
30	4180.0
31	5292.0
32	6989.0
33	10794.0
34	14627.0
35	18547.0
36	20241.0
37	32541.0
38	32950.0
39	20105.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.973022533357785	18.436579762046016	19.983927858737744	18.606469845858456
2	33.44623383347536	25.67066133085959	23.62928365711327	17.25382117855178
3	30.219940241837186	24.1982806044851	29.298219120454768	16.283560033222948
4	27.387360177764354	24.04996386464884	30.464797695966862	18.097878261619943
5	22.09110423161142	36.9993635863527	24.716580193512897	16.19295198852298
6	25.45654589189597	34.75249981123324	27.454776877689923	12.33617741918086
7	46.678783694866624	42.671535051290626	7.955170591217492	2.69451066262526
8	79.90928408858015	5.563765411457603	11.474861660931753	3.052088839030494
9	73.55431628679604	7.867798548113951	12.807015651461054	5.77086951362896
10	44.15254511525559	24.063447204633952	19.450526928926617	12.333480751183837
11	35.45039748886276	23.499843593256173	25.48620923986322	15.563549678017841
12	32.48298402493879	21.62512000172587	29.09489035347924	16.797005619856105
13	23.844477763275695	26.65224848177592	31.07424466329403	18.429029091654353
14	19.696463050255105	29.213543745348247	31.151908701608296	19.938084502788357
15	19.400908237781398	24.7408502054861	38.661589739717606	17.196651817014896
16	21.41801589955451	22.191420281100672	37.18920901334311	19.201354806001707
17	21.31985718446288	22.509087771149964	31.93286375354612	24.238191290841037
18	23.523574271629975	24.229022619651158	31.54346489477601	20.703938213942852
19	25.0941137130961	25.23487978254069	29.26801643888811	20.402990065475098
20	26.8388579071699	24.290506649983282	31.073165996095227	17.79746944675159
21	23.86982644244771	26.42141370123076	30.065151498808074	19.643608357513457
22	23.134175412859868	23.386044203781807	30.849881885941734	22.629898497416594
23	22.87745261954329	26.085408868801707	30.48259570474721	20.554542806907786
24	22.730214546905845	23.121770740073565	33.712125297981814	20.435889415038776
25	20.81180493382377	25.0471916899479	32.548243390466745	21.592759985761592
26	20.6154875036405	27.87491775162609	30.38929099205022	21.120303752683185
27	21.125157755077826	28.360857324689615	30.431898346403187	20.08208657382938
28	18.899867323934547	26.483437065162285	34.26494223737151	20.351753373531665
29	20.983313018434423	25.113529722674667	33.19005037375818	20.713106885132728
30	20.97738034884097	27.300527468260217	31.501396874022458	20.22069530887635
31	24.670197503964104	25.31631915605078	28.829538222572186	21.183945117412925
32	25.61510997012092	26.81890256399193	28.427734691015782	19.13825277487137
33	23.24042413194257	27.605790285523206	27.576126937555955	21.577658644978264
34	21.771818740763912	27.316707476242353	29.303073122849405	21.608400660144326
35	21.944405492573377	27.625745628701175	28.906662927287048	21.523185951438403
36	23.126085408868803	28.57281542925561	28.169933230500394	20.131165931375193
37	20.644072184408945	30.996041291380372	29.27287044128275	19.087016082927935
38	21.04102171357071	29.687078645625466	26.642540476986635	22.629359163817185
39	22.581897807069584	28.298833960758085	28.522118070911585	20.597150161260746
40	22.805721250822483	27.0270853333621	28.655872803563913	21.511320612251502
41	20.170537284131726	26.95049996224665	29.520963897008855	23.35799885661277
42	21.526961286634236	26.299524307765328	28.607332779617504	23.566181625982935
43	22.71673120692073	26.30276030936175	27.93154777956357	23.04896070415395
44	21.08794373671891	27.720129008596977	28.44391469899792	22.748012555686195
45	20.411619403065572	29.183341063781594	27.77460170213684	22.630437831015996
46	22.214072292275663	29.17956572858576	27.217470093951913	21.388891885186663
47	20.480114770189953	27.9822451379076	30.08133150679021	21.456308585112236
48	22.667112515775507	25.60378396453342	30.086724842784257	21.642378676906816
49	20.39220339348701	26.219702935053448	30.775993182823303	22.61210048863624
50	19.618799011940848	27.49253022964825	30.7754538492239	22.11321690918701
51	20.312921354374534	27.33126948342628	28.23303526163073	24.122773900568458
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	230.0
1	480.0
2	730.0
3	2334.5
4	3939.0
5	2730.0
6	1521.0
7	1354.0
8	1187.0
9	1133.0
10	1079.0
11	1069.0
12	1059.0
13	1017.5
14	976.0
15	944.5
16	913.0
17	893.5
18	874.0
19	853.5
20	833.0
21	893.5
22	954.0
23	967.0
24	980.0
25	1194.0
26	1666.5
27	1925.0
28	2406.5
29	2888.0
30	3211.5
31	3535.0
32	3847.5
33	4160.0
34	4660.0
35	5160.0
36	5460.0
37	5760.0
38	6536.5
39	7313.0
40	8495.0
41	9677.0
42	11415.5
43	13154.0
44	14521.0
45	15888.0
46	14746.5
47	13605.0
48	13131.0
49	12657.0
50	11831.0
51	11005.0
52	10440.5
53	9876.0
54	9538.5
55	9201.0
56	8892.5
57	8584.0
58	8368.5
59	8153.0
60	7768.5
61	7384.0
62	6786.0
63	6188.0
64	5304.0
65	4420.0
66	3681.5
67	2943.0
68	2465.0
69	1987.0
70	1648.5
71	1310.0
72	1037.5
73	765.0
74	639.0
75	408.0
76	303.0
77	235.5
78	168.0
79	138.0
80	108.0
81	76.5
82	45.0
83	34.5
84	24.0
85	16.5
86	9.0
87	11.0
88	13.0
89	10.0
90	7.0
91	4.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	185414.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.00445489553108
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.40600831272688	28.459555373380653
2	8.827011101173252	7.238935571208215
3	4.689061924554112	5.768172845631938
4	3.3816488662071866	5.5465067362766565
5	2.768716788551586	5.676486133733159
6	2.147892881569948	5.284390606966033
7	1.688851475772084	4.847530391448326
8	1.4297364129005103	4.69004498042219
9	1.1771978744673015	4.344332143203857
>10	4.445730520334614	25.12485572826216
>50	0.02367548797811333	0.6569083240747733
>100	0.011837743989056664	1.2695912929983713
>500	0.0013153048876729627	0.3872415243724853
>1k	0.0013153048876729627	0.7054483480211851
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1308	0.7054483480211851	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	718	0.3872415243724853	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCG	450	0.24270011973205907	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGC	337	0.18175542299934203	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTC	316	0.17042941741184592	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	293	0.1580247446255407	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCG	279	0.15047407423387663	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGC	217	0.11703539107079293	No Hit
GAATCTATCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTC	215	0.11595672387198377	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.028045347169037938	0.0
2	0.0	0.0	0.0	0.16611474861660933	0.0
3	0.0	0.0	0.0	0.20171076617731132	0.0
4	0.0	0.0	0.0	0.2842288068862114	0.0
5	0.0	0.0	0.0	0.6606836592706052	0.0
6	0.0	0.0	0.0	0.7890450559288943	0.0
7	0.0	0.0	0.0	0.9713398125276409	0.0
8	0.0	0.0	0.0	1.3887840184667823	0.0
9	0.0	0.0	0.0	1.5344040903060179	0.0
10	0.0	0.0	0.0	2.3164378094426525	0.0
11	0.0	0.0	0.0	2.7322640145835804	0.0
12	0.0	0.0	0.0	3.4501170353910706	0.0
13	0.0	0.0	0.0	3.606523779218398	0.0
14	0.0	0.0	0.0	3.685266484731466	0.0
15	0.0	0.0	0.0	3.850302566149266	0.0
16	0.0	0.0	0.0	4.118890698652745	0.0
17	0.0	0.0	0.0	4.4085128415330015	0.0
18	0.0	0.0	0.0	4.728876999579319	0.0
19	0.0	0.0	0.0	4.933284433753654	0.0
20	0.0	0.0	0.0	5.152253875111912	0.0
21	0.0	0.0	0.0	5.413830670823131	0.0
22	0.0	0.0	0.0	5.708846149697433	0.0
23	0.0	0.0	0.0	5.989299621387813	0.0
24	0.0	0.0	0.0	6.247640415502605	0.0
25	0.0	0.0	0.0	6.468227857659077	0.0
26	0.0	0.0	0.0	6.656455283851273	0.0
27	0.0	0.0	0.0	6.8786607268059585	0.0
28	0.0	0.0	0.0	7.084146828179102	0.0
29	0.0	0.0	0.0	7.3284649487093745	0.0
30	0.0	0.0	0.0	7.599210415610472	0.0
31	0.0	0.0	0.0	7.844067869740149	0.0
32	0.0	0.0	0.0	8.11049866784601	0.0
33	0.0	0.0	0.0	8.345108783587	0.0
34	0.0	0.0	0.0	8.603988911301196	0.0
35	0.0	0.0	0.0	8.860711704617774	0.0
36	0.0	0.0	0.0	9.102872490750428	0.0
37	0.0	0.0	0.0	9.363370619262838	0.0
38	0.0	0.0	0.0	9.616318077383585	0.0
39	0.0	0.0	0.0	9.879512873893018	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	85	0.0	45.000004	1
ACACGTT	25	3.878116E-5	45.0	20
ACGCCGG	20	7.017971E-4	45.0	27
CGTGAGT	20	7.017971E-4	45.0	32
CCGTGTC	25	3.878116E-5	45.0	38
CGACCGG	20	7.017971E-4	45.0	33
AAGGTCC	20	7.017971E-4	45.0	6
TCGATAG	20	7.017971E-4	45.0	2
ATCCGGA	25	3.878116E-5	45.0	39
TAACCTC	20	7.017971E-4	45.0	19
ATGCGGC	20	7.017971E-4	45.0	12
ACCCGTG	35	1.2045712E-7	45.0	36
GGTTCGT	20	7.017971E-4	45.0	10
CGTCATA	40	6.7611836E-9	45.0	38
TTACACG	20	7.017971E-4	45.0	34
AGTACGG	20	7.017971E-4	45.0	2
TACACGT	25	3.878116E-5	45.0	19
GTACGGG	35	1.2045712E-7	45.0	3
ACTGGAC	25	3.878116E-5	45.0	17
CGTTTTA	290	0.0	43.448273	1
>>END_MODULE