##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934706.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	310067
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.980765447467807	33.0	31.0	34.0	30.0	34.0
2	32.1581109889153	33.0	31.0	34.0	30.0	34.0
3	32.002757468547124	33.0	31.0	34.0	30.0	34.0
4	35.77976372848449	37.0	35.0	37.0	35.0	37.0
5	31.996220171769327	37.0	35.0	37.0	0.0	37.0
6	33.91202546546391	37.0	35.0	37.0	18.0	37.0
7	20.896464312551803	32.0	0.0	37.0	0.0	37.0
8	28.17063086365205	35.0	17.0	37.0	17.0	37.0
9	34.929270125489005	37.0	32.0	39.0	32.0	39.0
10	36.22823131774746	37.0	35.0	39.0	32.0	39.0
11	36.62582925625752	38.0	35.0	39.0	33.0	39.0
12	36.98330038346551	39.0	37.0	39.0	33.0	39.0
13	36.746390296290805	39.0	37.0	39.0	33.0	39.0
14	38.05135019205527	40.0	37.0	41.0	33.0	41.0
15	38.23417519439347	40.0	38.0	41.0	33.0	41.0
16	38.34111659738057	40.0	38.0	41.0	34.0	41.0
17	38.320991914650705	40.0	37.0	41.0	34.0	41.0
18	38.22934720560395	40.0	37.0	41.0	34.0	41.0
19	38.15780782863059	40.0	37.0	41.0	34.0	41.0
20	38.12286054304392	40.0	37.0	41.0	34.0	41.0
21	38.01280045925558	40.0	37.0	41.0	33.0	41.0
22	38.01103954951672	40.0	37.0	41.0	34.0	41.0
23	37.942128636714	40.0	36.0	41.0	33.0	41.0
24	37.82917885489265	40.0	36.0	41.0	33.0	41.0
25	37.67628609300571	40.0	36.0	41.0	33.0	41.0
26	37.56441672283732	39.0	36.0	41.0	33.0	41.0
27	37.5278181812318	39.0	35.0	41.0	33.0	41.0
28	37.45240867296423	39.0	35.0	41.0	33.0	41.0
29	37.44049834390632	40.0	35.0	41.0	33.0	41.0
30	37.26444929644238	39.0	35.0	41.0	32.0	41.0
31	37.118980736421484	39.0	35.0	41.0	32.0	41.0
32	36.9924790448516	39.0	35.0	41.0	31.0	41.0
33	36.85357358248378	39.0	35.0	41.0	31.0	41.0
34	36.647273008736825	39.0	35.0	41.0	30.0	41.0
35	36.486585157401464	39.0	35.0	41.0	30.0	41.0
36	36.319959879638915	39.0	35.0	41.0	30.0	41.0
37	36.250778057645604	39.0	35.0	41.0	30.0	41.0
38	36.095650294936256	39.0	35.0	41.0	29.0	41.0
39	35.985686964430265	39.0	35.0	40.0	29.0	41.0
40	35.89556773213531	39.0	35.0	40.0	28.0	41.0
41	35.74204607391306	39.0	35.0	40.0	27.0	41.0
42	35.63280194280591	39.0	35.0	40.0	27.0	41.0
43	35.561965639684324	38.0	35.0	40.0	27.0	41.0
44	35.43911799707805	38.0	35.0	40.0	26.0	41.0
45	35.352568960902	38.0	34.0	40.0	26.0	41.0
46	35.234765389415834	38.0	34.0	40.0	26.0	41.0
47	35.19081037324191	38.0	34.0	40.0	26.0	41.0
48	35.086268451657226	38.0	34.0	40.0	25.0	41.0
49	34.96697487962279	38.0	34.0	40.0	25.0	41.0
50	34.80229434283558	38.0	34.0	40.0	24.0	41.0
51	32.99294991082572	36.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	7.0
10	16.0
11	10.0
12	6.0
13	4.0
14	20.0
15	13.0
16	26.0
17	65.0
18	138.0
19	196.0
20	418.0
21	644.0
22	997.0
23	1614.0
24	2309.0
25	3181.0
26	3944.0
27	4327.0
28	4695.0
29	5511.0
30	6413.0
31	8201.0
32	10963.0
33	15690.0
34	21695.0
35	27438.0
36	35507.0
37	58432.0
38	59384.0
39	38192.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.77398110730907	17.56620343345148	22.238096927438264	17.421718531801194
2	31.402567832113704	24.268303302189526	26.222397094821442	18.106731770875324
3	32.309791109663394	23.212724991695342	28.065869634627354	16.411614264013906
4	27.991692118155107	26.06726933211209	27.35376547649379	18.58727307323901
5	21.689505816484825	37.043929215298625	24.126075977127524	17.140488991089022
6	26.066946821170912	34.77667729877736	25.829256257518534	13.327119622533196
7	49.66313732193365	42.47920610706718	5.517517181770391	2.3401393892287796
8	85.70405751015103	4.048802355619915	7.890552686999906	2.3565874472291473
9	80.88316396133739	5.991608265310401	9.353139805267894	3.772087968084317
10	42.061231927293136	27.13091041613587	17.455582180625477	13.352275475945522
11	31.28227125105219	26.59360718812386	25.50287518504065	16.6212463757833
12	29.07597390241464	24.624677892197493	28.509322178754914	17.790026026632955
13	24.907197476674398	25.668000786926697	30.018350872553352	19.406450863845556
14	21.74175258895658	26.50717425588663	30.78366933598222	20.967403819174564
15	20.196925180686755	26.546198079769855	33.17444294297684	20.082433796566548
16	25.164561207738974	25.459336207980854	30.6240264200963	18.75207616418387
17	25.149725704444524	25.50997042574669	28.666385007111366	20.673918862697416
18	25.697994304456778	24.56307830243141	29.799688454430818	19.939238938680994
19	24.69982294149329	26.283996684587525	28.03910122650911	20.977079147410077
20	26.28012655329332	26.493306285415734	28.815062550997045	18.411504610293903
21	25.189717061151303	27.33086719966975	29.50201085571828	17.97740488346067
22	25.23357854915228	24.650156256551004	29.87612354749135	20.24014164680537
23	23.660692688999475	26.462345235062102	30.300870457030253	19.57609161890817
24	22.654781063447576	25.65445532739698	31.495128472233418	20.195635136922018
25	23.19305182428314	26.766150541657126	29.20304321324101	20.837754420818726
26	22.491913038149818	27.57146036179277	28.613815723698426	21.32281087635898
27	21.66402745213131	27.535984158262565	30.252816326793884	20.54717206281223
28	20.295936039630146	27.881393376270292	30.432454921033198	21.39021566306637
29	21.61145816871837	27.018676608603947	30.13509983326184	21.234765389415834
30	22.626077589682232	26.72293407553851	30.2347557140876	20.41623262069166
31	23.740352891471844	26.378492390354342	29.096614602650394	20.78454011552342
32	23.55523161123241	27.275072806844975	28.502549448990056	20.667146132932558
33	23.64005198876372	26.484921000944954	28.045873956273965	21.829153054017358
34	21.3540944376538	26.982877894132557	30.310545785265763	21.35248188294788
35	22.078776522493527	27.418912686612895	29.84903262843192	20.65327816246166
36	23.182408963224077	28.843121002880025	27.254754617550404	20.7197154163455
37	21.552761177422944	29.155634104887007	27.997497315096414	21.29410740259363
38	22.15263152802459	28.810869908761656	28.35903208016332	20.677466483050438
39	21.81238248507581	28.232607791219316	27.875588179328986	22.079421544375894
40	24.22282925948263	26.665204617066635	28.553183666755892	20.558782456694843
41	21.322165854476612	26.33463090235336	28.80022704770259	23.54297619546743
42	21.728852151309233	26.680362631302266	28.73443481570112	22.85635040168738
43	21.353771926712614	27.627577265558735	28.15810776380589	22.860543043922764
44	21.411501385184494	27.797540531562532	28.355806970751484	22.43515111250149
45	21.504062025304208	28.021685635685188	27.447616160378242	23.02663617863236
46	21.678217933543394	28.298400023220786	27.19702515907852	22.826356884157295
47	20.706492467756966	27.995562249449314	29.13467089371007	22.16327438908365
48	21.383442933301513	27.599841324616936	29.375586566774274	21.64112917530727
49	22.603824334740555	27.268622588021294	28.438692282635685	21.68886079460246
50	21.15188007753163	27.932995126859677	28.7428201001719	22.172304695436793
51	20.921284754585297	27.608549120028897	28.09683068498099	23.373335440404816
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	197.0
1	546.5
2	896.0
3	2577.0
4	4258.0
5	2948.0
6	1638.0
7	1439.0
8	1240.0
9	1192.0
10	1144.0
11	1147.5
12	1151.0
13	1168.0
14	1185.0
15	1147.0
16	1109.0
17	1089.0
18	1069.0
19	1147.5
20	1226.0
21	1426.5
22	1627.0
23	1730.0
24	1833.0
25	2027.0
26	2793.5
27	3366.0
28	4142.0
29	4918.0
30	5770.5
31	6623.0
32	7688.0
33	8753.0
34	9632.5
35	10512.0
36	11162.0
37	11812.0
38	13024.0
39	14236.0
40	15399.5
41	16563.0
42	17908.0
43	19253.0
44	20380.0
45	21507.0
46	22120.5
47	22734.0
48	22303.5
49	21873.0
50	20852.0
51	19831.0
52	18906.5
53	17982.0
54	17461.5
55	16941.0
56	16365.0
57	15789.0
58	14872.5
59	13956.0
60	12740.0
61	11524.0
62	10484.5
63	9445.0
64	8303.5
65	7162.0
66	6166.0
67	5170.0
68	4349.5
69	3529.0
70	2935.0
71	2341.0
72	1924.0
73	1507.0
74	1208.0
75	699.5
76	490.0
77	375.5
78	261.0
79	194.0
80	127.0
81	106.0
82	85.0
83	57.0
84	29.0
85	27.5
86	26.0
87	18.5
88	11.0
89	6.5
90	2.0
91	3.5
92	5.0
93	3.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	310067.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.550203590530145
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.14168439656801	30.357259128687563
2	9.93896465545939	8.855657977595985
3	5.426455002973056	7.252490254719017
4	3.9549112717625907	7.047684093580236
5	2.9532925444549125	6.578489205893058
6	2.323308705028091	6.210232548759144
7	1.7955374641544466	5.5994091707753375
8	1.3602919828102256	4.848102782140919
9	1.0450445403357425	4.1901252329816385
>10	3.044320384946243	17.72693801436246
>50	0.011037989664064607	0.33078025929674926
>100	0.0036793298880215355	0.28586757790561484
>500	7.358659776043072E-4	0.2530845987879985
>1k	7.358659776043072E-4	0.4638791545142718
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1415	0.45635298177490674	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	772	0.2489784465938007	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0190281455298371	0.0
2	0.0	0.0	0.0	0.0728874727075116	0.0
3	0.0	0.0	0.0	0.09901085894338965	0.0
4	0.0	0.0	0.0	0.1312619530617576	0.0
5	0.0	0.0	0.0	0.2376905636523719	0.0
6	0.0	0.0	0.0	0.34637675083127195	0.0
7	0.0	0.0	0.0	0.45699800365727405	0.0
8	0.0	0.0	0.0	0.7540305804874431	0.0
9	0.0	0.0	0.0	0.8959353946082621	0.0
10	0.0	0.0	0.0	1.1626519429671651	0.0
11	0.0	0.0	0.0	1.479035176268355	0.0
12	0.0	0.0	0.0	1.7293036666268904	0.0
13	0.0	0.0	0.0	1.8550829336885253	0.0
14	0.0	0.0	0.0	1.921842698513547	0.0
15	0.0	0.0	0.0	2.0050505213389362	0.0
16	0.0	0.0	0.0	2.1701761232249805	0.0
17	0.0	0.0	0.0	2.3649727316999227	0.0
18	0.0	0.0	0.0	2.582345106057723	0.0
19	0.0	0.0	0.0	2.7116719934723785	0.0
20	0.0	0.0	0.0	2.8622846030051567	0.0
21	0.0	0.0	0.0	3.064498963127324	0.0
22	0.0	0.0	0.0	3.290901643838267	0.0
23	0.0	0.0	0.0	3.5208519449022306	0.0
24	0.0	0.0	0.0	3.701780582906275	0.0
25	0.0	0.0	0.0	3.876904023969013	0.0
26	0.0	0.0	0.0	4.040417071149139	0.0
27	0.0	0.0	0.0	4.2210231982119994	0.0
28	0.0	0.0	0.0	4.406789500333799	0.0
29	0.0	0.0	0.0	4.6077138166912315	0.0
30	0.0	0.0	0.0	4.886685780815114	0.0
31	0.0	0.0	0.0	5.129859030467609	0.0
32	0.0	0.0	0.0	5.350456514237245	0.0
33	0.0	0.0	0.0	5.549445764947576	0.0
34	0.0	0.0	0.0	5.767463161187743	0.0
35	0.0	0.0	0.0	6.023536848487585	0.0
36	0.0	0.0	0.0	6.251229572963263	0.0
37	0.0	0.0	0.0	6.488597625674451	0.0
38	0.0	0.0	0.0	6.763054436621762	0.0
39	0.0	0.0	0.0	7.200379272866832	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGGA	20	7.025309E-4	45.000004	1
ATTCGCG	20	7.025309E-4	45.000004	39
GAATACG	20	7.025309E-4	45.000004	22
ACGGGTA	20	7.025309E-4	45.000004	5
GGCTTAT	30	2.1603573E-6	45.000004	8
CGGATGT	20	7.025309E-4	45.000004	45
ACGTAAG	20	7.025309E-4	45.000004	2
ATACGCA	20	7.025309E-4	45.000004	24
AATACGC	20	7.025309E-4	45.000004	23
CCATTCG	20	7.025309E-4	45.000004	37
ATACTCG	25	3.8841954E-5	45.0	44
GTGCGTA	25	3.8841954E-5	45.0	1
CACGACC	65	0.0	44.999996	27
AGGCGAT	150	0.0	42.0	7
TCAAGCG	75	0.0	42.0	17
AACACGT	65	0.0	41.53846	41
CGTTTTA	270	0.0	40.833332	1
ACGGGCT	45	1.920489E-8	40.000004	5
ATAGGAT	80	0.0	39.375004	5
GGACCGA	155	0.0	39.193546	8
>>END_MODULE