##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934705.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	263614
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00224570773935	33.0	31.0	34.0	30.0	34.0
2	32.198115426343065	33.0	31.0	34.0	30.0	34.0
3	32.051344010560896	33.0	31.0	34.0	30.0	34.0
4	35.8278581562436	37.0	35.0	37.0	35.0	37.0
5	32.0833908669494	37.0	35.0	37.0	0.0	37.0
6	33.98126806618768	37.0	35.0	37.0	19.0	37.0
7	20.762341909003315	32.0	0.0	37.0	0.0	37.0
8	28.11824865143733	35.0	17.0	37.0	17.0	37.0
9	34.9206832717534	37.0	32.0	39.0	32.0	39.0
10	36.288478608875096	37.0	35.0	39.0	32.0	39.0
11	36.70780383439423	38.0	35.0	39.0	33.0	39.0
12	37.00706715121352	39.0	37.0	39.0	34.0	39.0
13	36.676648432936034	39.0	35.0	39.0	33.0	39.0
14	37.94366384182934	40.0	37.0	41.0	33.0	41.0
15	38.178416169095726	40.0	37.0	41.0	33.0	41.0
16	38.31266169475066	40.0	37.0	41.0	34.0	41.0
17	38.31293861479284	40.0	37.0	41.0	34.0	41.0
18	38.22226816481674	40.0	37.0	41.0	34.0	41.0
19	38.15901659244198	40.0	37.0	41.0	34.0	41.0
20	38.10954274052213	40.0	37.0	41.0	34.0	41.0
21	38.007215094797694	40.0	36.0	41.0	33.0	41.0
22	38.028723815882316	40.0	36.0	41.0	34.0	41.0
23	37.97819918517226	40.0	36.0	41.0	34.0	41.0
24	37.84093029960472	40.0	36.0	41.0	33.0	41.0
25	37.714260244144846	40.0	35.0	41.0	33.0	41.0
26	37.5943045513516	40.0	35.0	41.0	33.0	41.0
27	37.548332030924	40.0	35.0	41.0	33.0	41.0
28	37.481359108393335	40.0	35.0	41.0	33.0	41.0
29	37.455840736834915	40.0	35.0	41.0	33.0	41.0
30	37.29718072636506	39.0	35.0	41.0	33.0	41.0
31	37.17145144036356	39.0	35.0	41.0	32.0	41.0
32	37.048715166872775	39.0	35.0	41.0	32.0	41.0
33	36.888807119500484	39.0	35.0	41.0	31.0	41.0
34	36.71641870310378	39.0	35.0	41.0	31.0	41.0
35	36.5865318230443	39.0	35.0	41.0	30.0	41.0
36	36.40706866858361	39.0	35.0	41.0	30.0	41.0
37	36.315309505564954	39.0	35.0	41.0	30.0	41.0
38	36.17808234767501	39.0	35.0	41.0	30.0	41.0
39	36.062773600795104	39.0	35.0	40.0	29.0	41.0
40	35.925079851601204	39.0	35.0	40.0	28.0	41.0
41	35.83759967224806	39.0	35.0	40.0	28.0	41.0
42	35.744046219093065	39.0	35.0	40.0	27.0	41.0
43	35.678556525829435	38.0	35.0	40.0	27.0	41.0
44	35.53304073380018	38.0	35.0	40.0	27.0	41.0
45	35.398468973575	38.0	34.0	40.0	27.0	41.0
46	35.32511171637318	38.0	34.0	40.0	26.0	41.0
47	35.275266108780265	38.0	34.0	40.0	26.0	41.0
48	35.15859931566609	38.0	34.0	40.0	26.0	41.0
49	35.05392733314619	38.0	34.0	40.0	26.0	41.0
50	34.886618313139664	38.0	34.0	40.0	25.0	41.0
51	33.06768987990016	36.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	7.0
9	17.0
10	3.0
11	5.0
12	9.0
13	10.0
14	11.0
15	10.0
16	23.0
17	50.0
18	78.0
19	150.0
20	302.0
21	507.0
22	837.0
23	1151.0
24	1870.0
25	2529.0
26	3133.0
27	3657.0
28	3925.0
29	4346.0
30	5402.0
31	7119.0
32	9399.0
33	13832.0
34	18990.0
35	24360.0
36	29575.0
37	48653.0
38	51194.0
39	32454.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.11693612630589	18.3715584149552	23.480922864491262	17.03058259424765
2	31.116329178268227	24.429279173336774	26.456864961648467	17.997526686746532
3	31.57116086399053	23.431987678954837	28.507590643896	16.489260813158634
4	27.367666360663694	25.568824114045537	27.567959213091868	19.495550312198898
5	22.442662377567203	37.61484594900119	23.36560273733565	16.57688893609596
6	25.396223265835655	34.81529812528925	25.87191879035256	13.916559818522536
7	49.36042850531459	42.96623092855463	5.2573080337159634	2.416032532414819
8	85.32361710683044	4.234221247733429	7.680927416601547	2.7612342288345837
9	80.65998012245177	6.029649411639746	9.438421328154044	3.871949137754444
10	42.41694295447131	26.489488418672757	17.13224639055589	13.961322236300045
11	33.491392718140915	24.850728716987717	24.278300848968566	17.379577715902798
12	30.738883367347714	23.222969948485286	28.198426487212362	17.83972019695464
13	24.332926172358068	27.135508736258316	30.034823643660808	18.496741447722805
14	20.1241208737017	28.656672255646516	30.696017662187895	20.52318920846389
15	19.008095169452304	25.18151539751303	35.471940033533876	20.338449399500785
16	22.843627424947083	23.81322691511073	32.89961838142132	20.443527278520868
17	23.014710903062813	24.173602312472024	28.572078872897492	24.23960791156767
18	24.842762523993414	23.97824091284985	30.908828817892832	20.270167745263908
19	25.604482311258124	25.051400911939425	27.682141312676865	21.661975464125575
20	26.40148095321189	25.51495747570311	28.721539827171544	19.36202174391345
21	24.354928038723283	26.615050793963903	29.18092362317631	19.8490975441365
22	24.966807529190408	23.20741690502022	29.08419127967407	22.741584286115305
23	21.981002526421207	26.11849143065239	29.704036963135493	22.196469079790905
24	21.516687277610448	25.383704962558895	33.10332531656134	19.996282443269326
25	21.97948515632705	26.54790716729764	29.856912000121387	21.615695676253914
26	21.33839629154749	27.631309414522747	28.574734270562264	22.4555600233675
27	20.725378773509753	26.66588269211802	30.91034618798698	21.698392346385244
28	18.909086770808834	26.956459065148287	31.010113271677533	23.124340892365353
29	20.480323503304074	26.386307252270363	30.55869566866707	22.574673575758496
30	21.159346620437457	27.41773957377074	31.137572359586365	20.285341446205436
31	24.094699067576077	26.975426191325198	28.407444217681917	20.522430523416816
32	24.282473616727486	26.825206552004065	28.16694105775869	20.725378773509753
33	23.70587298094942	26.766408460855644	28.08272701753321	21.444991540661725
34	20.478047448162844	25.911370412800533	30.75443641081278	22.856145728223844
35	21.536413088834433	28.31336727184444	28.946110601106163	21.204109038214966
36	24.206984454543385	27.80618631787386	27.166994165711987	20.819835061870766
37	21.073235867594285	28.74619709120153	28.767440272519668	21.413126768684513
38	21.839887107664996	28.681708862200033	28.117626529698725	21.360777500436242
39	21.346362484541793	28.007996540396185	28.572458215421033	22.073182759640993
40	24.04614322456319	25.4284673803364	28.50531458875477	22.02007480634564
41	20.862321424507044	24.819243287534047	29.29510572276131	25.0233295651976
42	21.246216058327708	25.4830927037259	29.186613761029385	24.084077476917006
43	21.586486301941473	25.65834894960055	29.198752721782604	23.556412026675368
44	21.531102293504897	26.652985046317724	28.696503220618023	23.119409439559355
45	20.49094509396314	27.695418301000707	27.538370496255887	24.27526610878026
46	21.49203001358046	28.162768289999775	27.46288133407179	22.88232036234798
47	21.018610544204787	27.04370784556207	29.617167525245247	22.3205140849879
48	21.104721297047956	26.58166865189254	29.79356179869051	22.520048252368994
49	21.476476970115396	26.788410327220863	29.908123240799046	21.826989461864695
50	20.714757182850683	27.91164353941748	29.565956284567584	21.807642993164247
51	20.155226960631833	27.083538810533582	28.269363539114007	24.491870689720578
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	159.0
1	485.5
2	812.0
3	1974.5
4	3137.0
5	2145.5
6	1154.0
7	1034.0
8	914.0
9	907.0
10	900.0
11	921.0
12	942.0
13	905.0
14	868.0
15	828.0
16	788.0
17	769.5
18	751.0
19	819.5
20	888.0
21	920.0
22	952.0
23	1220.5
24	1489.0
25	1879.5
26	2589.0
27	2908.0
28	3348.0
29	3788.0
30	4488.0
31	5188.0
32	6060.0
33	6932.0
34	7962.5
35	8993.0
36	9871.0
37	10749.0
38	11502.5
39	12256.0
40	13501.0
41	14746.0
42	15933.0
43	17120.0
44	18020.5
45	18921.0
46	19170.0
47	19419.0
48	19049.5
49	18680.0
50	18034.0
51	17388.0
52	16602.0
53	15816.0
54	15165.0
55	14514.0
56	13820.5
57	13127.0
58	12433.5
59	11740.0
60	10706.0
61	9672.0
62	8803.5
63	7935.0
64	6953.5
65	5972.0
66	5142.5
67	4313.0
68	3554.0
69	2795.0
70	2314.0
71	1833.0
72	1513.0
73	1193.0
74	955.0
75	595.0
76	473.0
77	341.0
78	209.0
79	154.5
80	100.0
81	68.0
82	36.0
83	27.0
84	18.0
85	18.5
86	19.0
87	14.0
88	9.0
89	8.0
90	7.0
91	5.0
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	263614.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.87495131178367
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.97149314572674	26.03513535317581
2	9.500221318135422	7.386412823873675
3	4.984360057512256	5.8130026366856455
4	3.5809673147307173	5.568397200369814
5	2.6793871893339998	5.208052326538807
6	2.2106634097243907	5.156365945186642
7	1.8268950581464045	4.971431950602576
8	1.5460648844915414	4.808255768757369
9	1.2692323211401562	4.4407210218899165
>10	5.409727994963022	29.338285845255108
>50	0.013991537396886744	0.32518665747341
>100	0.004996977641745265	0.3057609312205181
>500	9.99395528349053E-4	0.2276695116838928
>1k	9.99395528349053E-4	0.4153220272868284
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1069	0.40551715766233964	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	586	0.22229471879338733	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02958871683597988	0.0
2	0.0	0.0	0.0	0.1278384304323746	0.0
3	0.0	0.0	0.0	0.1801876986806467	0.0
4	0.0	0.0	0.0	0.2336749944995334	0.0
5	0.0	0.0	0.0	0.37251435811451594	0.0
6	0.0	0.0	0.0	0.50680161144704	0.0
7	0.0	0.0	0.0	0.6399508372089494	0.0
8	0.0	0.0	0.0	0.9775656831579507	0.0
9	0.0	0.0	0.0	1.108438853778631	0.0
10	0.0	0.0	0.0	1.4149476127974994	0.0
11	0.0	0.0	0.0	1.8269135933599885	0.0
12	0.0	0.0	0.0	2.1322843248082424	0.0
13	0.0	0.0	0.0	2.26998566085261	0.0
14	0.0	0.0	0.0	2.3303011220951846	0.0
15	0.0	0.0	0.0	2.442586509062493	0.0
16	0.0	0.0	0.0	2.69029717693294	0.0
17	0.0	0.0	0.0	3.0085655541814926	0.0
18	0.0	0.0	0.0	3.3385935496597297	0.0
19	0.0	0.0	0.0	3.560508925929579	0.0
20	0.0	0.0	0.0	3.776734164346355	0.0
21	0.0	0.0	0.0	4.043411958393712	0.0
22	0.0	0.0	0.0	4.333608988900438	0.0
23	0.0	0.0	0.0	4.645428543248841	0.0
24	0.0	0.0	0.0	4.885552360648524	0.0
25	0.0	0.0	0.0	5.1165719574832895	0.0
26	0.0	0.0	0.0	5.324072317858687	0.0
27	0.0	0.0	0.0	5.579749178723437	0.0
28	0.0	0.0	0.0	5.807354692846359	0.0
29	0.0	0.0	0.0	6.06037615604634	0.0
30	0.0	0.0	0.0	6.3885074389068865	0.0
31	0.0	0.0	0.0	6.639252846965639	0.0
32	0.0	0.0	0.0	6.880514691935937	0.0
33	0.0	0.0	0.0	7.130122072424075	0.0
34	0.0	0.0	0.0	7.4146289650777275	0.0
35	0.0	0.0	0.0	7.74769170074427	0.0
36	0.0	0.0	0.0	8.007920671891478	0.0
37	0.0	0.0	0.0	8.286737426692058	0.0
38	0.0	0.0	0.0	8.607281859081839	0.0
39	0.0	0.0	0.0	9.09701305696966	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAG	20	7.0233876E-4	45.0	2
ATAGGCG	35	1.2071723E-7	45.0	5
CACGGGC	45	3.8198777E-10	45.0	4
CGCGGGT	20	7.0233876E-4	45.0	4
GAGTACG	25	3.8826016E-5	45.0	1
CACGACC	70	0.0	45.0	27
GCGCAAA	20	7.0233876E-4	45.0	1
AATTGGC	20	7.0233876E-4	45.0	37
CACTAGG	50	2.1827873E-11	45.0	3
TACGACG	20	7.0233876E-4	45.0	1
TCTCGAC	20	7.0233876E-4	45.0	21
CAGGCGA	25	3.8826016E-5	45.0	6
CATCGAA	20	7.0233876E-4	45.0	42
CGGCTAG	20	7.0233876E-4	45.0	2
ATTATGC	20	7.0233876E-4	45.0	35
GTAAGCC	20	7.0233876E-4	45.0	9
CGATCTC	20	7.0233876E-4	45.0	13
CGTTTAG	25	3.8826016E-5	45.0	2
GACCTAT	20	7.0233876E-4	45.0	33
GTTATAG	20	7.0233876E-4	45.0	2
>>END_MODULE